A Synthetic Naringenin Derivative, 5-Hydroxy-7,4′-diacetyloxyflavanone-<i>N</i>-phenyl Hydrazone (N101-43), Induces Apoptosis through Up-regulation of Fas/FasL Expression and Inhibition of PI3K/Akt Signaling Pathways in Non-Small-Cell Lung Cancer Cells

Bak, Yesol,Kim, Heejong,Kang, Jeong-Woo,Lee, Dong Hun,Kim, Man Sub,Park, Yun Sun,Kim, Jung-Hee,Jung, Kang-Yeoun,Lim, Yoongho,Hong, Jintae,Yoon, Do-Young American Chemical Society 2011 Journal of agricultural and food chemistry Vol.59 No.18

<P>Naringenin, a well-known naturally occurring flavonone, demonstrates cytotoxicity in a variety of human cancer cell lines; its inhibitory effects on tumor growth have spurred interest in its therapeutic application. In this study, naringenin was derivatized to produce more effective small-molecule inhibitors of cancer cell proliferation, and the anticancer effects of its derivative, 5-hydroxy-7,4′-diacetyloxyflavanone-<I>N</I>-phenyl hydrazone (N101-43), in non-small-cell lung cancer (NSCLC) cell lines NCI-H460, A549, and NCI-H1299 were investigated. Naringenin itself possesses no cytotoxicity against lung cancer cells. In contrast, N101-43 inhibits proliferation of both NCI-H460 and A549 cell lines; this capacity is lost in p53-lacking NCI-H1299 cells. N101-43 induces apoptosis via sub-G<SUB>1</SUB> cell-cycle arrest in NCI-H460 and via G<SUB>0</SUB>/G<SUB>1</SUB> arrest in A549 cells. Expression of apoptosis and cell-cycle regulatory factors is altered: Cyclins A and D1 and phospho-pRb are down-regulated, but expression of CDK inhibitors such as p21, p27, and p53 is enhanced by N101-43 treatment; N101-43 also increases expression levels of the extrinsic death receptor Fas and its binding partner FasL. Furthermore, N101-43 treatment diminishes levels of cell survival factors such as PI3K and p-Akt dose-dependently, and N101-43 additionally induces cleavage of the pro-apoptotic factors caspase-3, caspase-8, and poly ADP-ribose polymerase (PARP). Cumulatively, these investigations show that the naringenin derivative N101-43 induces apoptosis via up-regulation of Fas/FasL expression, activation of caspase cascades, and inhibition of PI3K/Akt survival signaling pathways in NCI-H460 and A549 cells. In conclusion, these data indicate that N101-43 may have potential as an anticancer agent in NSCLC.</P>

Doppler ultrasound를 이용한 교정적 치아 이동 시 치수 혈류량의 변화의 측정 : 예비실험

임경섭,배영민,차정열,유형석,황충주 대한치과교정학회 2009 대한치과교정학회지 Vol.39 No.6

교정적 치아 이동 시 지속되는 압력에 의해 혈류변화가 발생하게 되며, 이러한 변화양상에 있어서 선학들의 연구 방법 및 그 결과의 다양성이 존재하였다. 본 연구에서는 Doppler ultrasound를 이용하여, 교정치료 전과 교정치료 시작 3주, 6주 후 치수 혈류량의 연속적인 변화를 알아보고자 하였다. 만 15세 이상 환자 18명을 연구대상으로 Doppler ultrasound를 이용하여 상, 하악 6전치 중 경도의 총생(2 mm 미만)을 보이는 치아(총생측정: required space-available space)와 인접치아를 포함한 3개의 치아를 대상으로 치수 혈류량의 변화를 알아보았다. 연구결과 부위별(상, 하악), 치아별, 기간에 따른 치수 혈류량의 변화는 교정 치료 시작 전과 시작 후 3주, 6주 혈류량에 유의한 차이가 없었다. 또한 치수 생활력 상실의 내재적 위험성을 비교하고자 교정 치료 시작 전 치아별로 치수혈류량을 비교하였을 때, 상악에서는 측절치, 하악에서는 견치에서 통계학적으로 유의한 차이는 나타나지 않았지만, 모든 항목에서 적은 값을 나타냈다 (p > 0.05). 본 연구의 결과는 이후에 진행될 Doppler ultrasound의 치아이동유형, 환자의 연령을 고려한 세부적인 실험 시 방법론적인 기초 자료로서 뿐만 아니라, 교정치료 시 치수 생활력의 상실에 대한 참고 자료가 될 수 있을 것이다. Objective: This study was to change of pulp blood flow among maxillary and mandibular anterior tooth with mild crowding and adjacent teeth using Ultrasound Doppler graphy. Methods: The change of pulp blood flow was measured three times using Ultrasound Doppler graphy; before the attachment of brackets, after 3 week, and after 6 week. The sample consists of 15 year old eighteen patients. Results: Before the attachment of brackets, after 3 weeks, and after 6 weeks, there were no significant differences in the change of pulp blood flow in each part (maxilla and mandible) and each tooth according to period. In addition, to compare internal dangerousness of loss of the pulp vitality, when pulp blood flow is compared in each tooth before orthodontic treatment, there were no statistically significant differences in maxillary lateral incisor and mandibular canine but it showed low values in all measurement items (p > 0.05). Conclusions: Results of this study can be not only methodological preliminary data in further study such as tooth movement type of Ultrasound Doppler graphy and particular study considered the patient age, but also reference materials for the loss of pulp vitality in orthodontic treatment.

운동처방실 소비자 만족도에 관한 연구

임정섭,서봉한 한국스포츠리서치 2001 한국 스포츠 리서치 Vol.12 No.4

The purpose of this study is to investigate problems and to find out solutions for efficient management of exercise prescription centers through the comparison of use patterns and satisfaction of using exercise prescription centers by sex and age. A group of 150 persons who are the members of two sports centers and two health centers located in Ulsan was the sample object of this research. In order to analyze use patterns including frequency, purpose, information acquisition, and selection motive by sex and age, a Chi-square test has been performed. Satisfaction to facility, number of members, and excercise program, and post satisfaction was analyzed by one-way ANOVA. Significance test was accepted at α=.05. The analysis of the satisfaction to the use of the exercise prescription centers by sex and age are as follows; First, the satisfaction related to facilities was insignificant between the groups of sex, but it was significant among the groups of age. For instance, under fifties were satisfied with facilities. Second, the satisfaction related to the number of center members was insignificant by sex or age. Third, the satisfaction related to the exercise program was insignificant by sex or age. Fourth, there was no significance for the post satisfaction by sex or age. In conclusion, the results of this study indicate that the centers should devise various exercise programs and improve existing services in order to increase members' satisfaction. In addition, the managers at the exercise prescription centers should be aware of consumer behavior, so that they could operate the centers more effectively with consumer satisfaction.

과두과증식을 동반한 안면비대칭 환자에서 과두절제술의 적용과 효과

임경섭,차정열,황충주 대한치과교정학회 2008 대한치과교정학회지 Vol.38 No.6

과두과증식은 하악의 3차원적인 골격적인 비대가 일어나는 병적인 상태이다. 이러한 과두과증식의 원인은 호르몬의 작용, 외상, 감염, 유전, 태아기 때의 요인, hypervascularity 등의 요인으로 알려져 있다. 과두과증식의 진단 시 가장 중요한 것은 과두과증식 상태가 아직도 활성화 상태인지 판단하는 것이다. 이러한 과두과증식의 상태에 따라서 안면비대칭 환자의 치료는 이환측 과두의 성장 부위를 제거하는 과두절제술을 시행하는 방법, 성장이 완료될 때까지 기다렸다가 통상적인 악교정 수술만을 시행하거나 과두절제술을 병행하는 시술방법이 있다. 과두과증식의 활성화 상태를 판단하는 것은 치료 안정성에 매우 중요한 요인이며, bone scan이나 주기적인 3차원 컴퓨터 단층촬영이나 정모두부방사선사진 등을 통하여 확인할 수 있다. 본 보고에서는 과두과증식을 동반한 안면비대칭 환자를 과두절제술을 이용하여 개선한 증례를 소개하고자 한다. Condylar hyperplasia is a pathologic condition showing 3-dimensional skeletal hyperplasia of the mandible. The reason for condylar hyperplasia is not yet known, but the effects of hormone, trauma, infection, genetics, fetal condition, and hypervascularity are known as possible reasons. When we diagnose a patient as having condylar hyperplasia, it is important to decide if it is in progress or not. Treatment for facial asymmetry due to condylar hyperplasia are decided accordingly, including condylectomy, that is removal of growth site of the affected condyle, and conventional orthognathic surgery only or condylectomy with orthognathic surgery after the completion of growth. Therefore, it is important to determine the growth state of condylar hyperplasia in treatment stability. This is verified through bone scan and regular check-ups with 3D CT or PA cephalogram. This case report introduces an improved case of facial asymmetry with condylectomy together with orthognathic surgery.

染料化學工場廢水의 電解處理에 관한 硏究

박정호,임찬섭,박승조 동아대학교 환경문제연구소 1996 硏究報告 Vol.19 No.2

The objective of this study was to investigate the feasibility for electrolytic treatment of dyes chemical factory wastewater. The expermental results are summarized as follows. 1. In case of the electrolytic treatment for dyes raw wastewater at current density of 1.82A/㎠ and 15minutes electrolysis duration, It was found that the COD removal efficiency and color were 35% and 240, respectively. 2. In case of the electrolytic treatment for dyes 1st treated wastewater at current density of 1.82A/㎠ and 15minutes electrolysis duration, It was found that the COD removal efficiency and color were 40% and 210, respectively. 3. In case of the electrolytic treatment of dyes raw wastewater at current density of l.82A/㎠ and l5minutes electrolysis duration, It was found that the COD removal efficiency and color were 65% and 220, respectively.

中溫 嫌氣性 消化에 의한 屠殺場 廢水處理

박정호,임찬섭,최장승,박승조 동아대학교 환경문제연구소 1996 硏究報告 Vol.19 No.1

Slaughterhouse wastewater containing high ammonium ion concentration and organic matter was treated by upflow anaerobic sludge blanket(UASB) reactor. As organic loading rate(OLR) was increased from 0.25㎏COD/㎥·day to 8.0㎏COD/㎥·day, COD removal efficiency was about 80 percent. But in case of OLR increased up to 8.0 ㎏COD/㎥·day, organic removal efficiency was decreased. In case of OLR was increased from 0.25㎏COD/㎥·day to 8.0㎏COD/ ㎥·day and ammonium loading rate was increased from 0.02 ㎏ NH₄^(+)-N/㎥·day to 0.62 ㎏ NH₄^(+)-N/㎥·day, effluent ammonium ion concentration was decreased from 1800㎎/l to 720㎎/l. Biogas production was increased form 0.248 ㎥/㎏COD_(rem) to 0.280 ㎥/㎏COD_(rem) in case of OLR was increased from 0.25 ㎏COD/㎥·day to 6.0 ㎏COD/㎥·day. Methane, carbon dioxide and ammonia gas content were 49%, 49% and 2.0% respectively in initial OLR was 0.25 ㎏COD/㎥·day but ammonia gas does not produced in case of OLR was increased up to 4.0 ㎏COD/㎥·day. In proportion to decrease hydraulic retention time, MLSS concentration in upflow anaerobic sludge blanket reactor was increased.

UASB에 의한 染料化學工場 廢水處理

김성우,임찬섭,박정호,성낙창,박승조 동아대학교 환경문제연구소 1996 硏究報告 Vol.19 No.1

This study has been carried out to measuring the biodegradability and toxicity of dyes chemnical factory wastwwater for anaerobic treatment. The experiment consisted of batch test pilot plant test. The results are as follows 1. Biodegradability assays were conducted at two assay concentration for 2gCOD and 5gCOD tat BMP are respectively 0.23㎥CH₄/ ㎏.COD, 0.16㎥CH₄/㎏.COD respresents the noninhibited bioconversion efficency also MRR were 1.080 and 0.959 represented. 2. In pilot plant experiment, COD removal efficency are up 65% represented according to changing COD loading rate from 0.15㎏COD/㎥.d to 2.6㎏COD/㎥.d 3. Alkalinity and Volatile, VA/Alkalinity as chemical factor of anaerobic was 1070∼1700㎎/ℓ, 30∼150g/ℓ and 0.02∼0.09 respectively. 4. According to changing COD loading rate from 0.15㎏COD/㎥.d to 2.6㎏/COD/㎥.d the methane gas production was 0.20~0.23]㎥ CH₄/㎏.COD_(rem)

파일럿 플랜트에 의한 음식물 찌꺼기 처리

최성우,류정숙,박정호,임찬섭,박승조 동아대학교 환경문제연구소 1998 硏究報告 Vol.21 No.1

In this study, using the extinctive composting apparatus, it took a day for the degradation of waste food with high temperature and aerobic condition. It was confirmed by the change of organic ratio, condensed water, and weight. Final production included rare organic ratio so that it was easy to degrade microorganism but it was not enough pH and salt for using compost. During reaction, condensed water was produced about 20 ℓ/day, the eighty percent of waste food was degraded by compositing reaction on the basis of weight, therefore, this means that it is go effective on the volume reduction.

EIA-709.1 Control Network Protocol을 이용한 필드버스 시스템 구현

최병욱,김정섭,이창희,김종배,임계영 제어·자동화·시스템공학회 2000 제어·자동화·시스템공학논문지 Vol.6 No.-

EIA-709.1 Control Network Protocol is the basic protocol of LonWorks systems that is emerging as a fieldbus device. In this paper, the protocol is implemented by using VHDL with FPGA and C program on an Intel 8051 processor. The protocol from the physical layer to the network layer of EIA-709.1 is implemented in a hardware leval. So it decreases the load of the CPU for implementing the protocol. We verify the commercial feasibility of the hardware through the communication test with Neuron Chip, based on EIA-709.1 protocol, which is used in industrial fields. The developed protocol based on FPGA becomes one of IP and we are able to implement SOC for the terminal device in the distributed control systems. Also, the result can be applicable to various industrial field because it is implemented by VHDL.

전위된 관절내 원위 요골의 관절경을 이용한 고정술

이원용,박병문,임대의,송경섭,홍정우 대한골절학회 2003 대한골절학회지 Vol.16 No.3

목적: 전위된 관절내 원위 요골 골절에 관절경을 이용한 정복술과 경피적 K 강선 및 외고정기기를 이용한 수술법의 결과를 알아보고자 함이다. 대상 및 방법: 2001년 1월부터 8월까지 수술한 11명 12예를 대상으로 하였으며, 평균 추시 기간은 14개월 이었다. 골절의 형태는 AO 분류에 의하면 1예는 B3형, 4예는 C2형, 7예는 C3형 골절이었다. 전예에서 2.7㎜ 관절경을 이용하였으며 추가적인 관혈적 정복은 시행하지 않았다. 경피적 K 강선 고정술 후 외고정 기기를 사용하였다. 관절내 골연골 골편을 제거하였으며 추가적인 수근 골간 인대와 삼각 섬유 연골 손상의 적극적인 처치는 없었다. 고정물 제거 시기는 경피적 K 강선은 술후 4주, 외고정 기기는 7주에 시행하였다. 결과: 평균 수근 관절의 신전 및 굴곡은 정상의 72%였다. 평균 요측 길이는 12.3 ㎜, 전방경사는 5.7°, 척측 경사는 22.4°였다. 평균 관절면의 불일치는 1㎜였다. 술후 구획 증후군의 발생은 없었으며 치료 중 골절 함몰 소견은 없었다. 임상적으로 Gartland와 Werley의 평가에 의하면 8예에서 우수, 3예에서 양호, 1예에서 보통의 결과를 얻었다. 결론: 관절내 분쇄 골절의 치료에서 관절경을 이용한 관절면 정복술은 수술적 연부 조직을 최소화하면서 관절면을 비교적 정확하게 정복함으로 관절면의 불일치를 감소시켜 손상 후 좋은 결과를 얻을 수 있는 효과적인 방법으로 사료된다. Purpose: To investigate the result of arthroscopically assisted reduction of intraarticular fracture of distal radius with percutaneous K-wires and external fixation. Materials and Methods: We reviewed 12 intraarticular distal radial fractures of 11 patients treated arthroscopically assisted reduction in Orthopedic Department of Sung-Ae hospital between January, 2001 and August, 2001. The mean length of follow-up was 14 months. Analysis of them revealed that B3 was 1 case, C2 4 cases and C3 7 cases according to the AO classification. All cases were treated by 2.7 ㎜ arthroscopic devices and percutaneous K-wires pining with external fixationg, but additionally invasive reductional technique was not used. We removed the osteochondral flap but not treated. The K-wires were removed at 4 weeks, external fixation was 7 weeks, respectivley. Result: The mean active range of movement in the affected wrist was total arc of the flextion-extension 72% of the opposite side. Radiographically mean volar tilt, radial inclination and radial length compartment syndrome and fractures collapse were not occured during follow-up period. According to the Gartland and Werley demerit-point system, eleven cases were a excellent or good one was a fair result. Conclusion: Arthroscopically assisted fixation is a useful method for reducing the soft tissue injuies and preventing the articular surface incogruency by anatomically reduction un cases of intraarticular comminution.