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Lim, Jung-Gu,Lee, Joong-Gi,Kim, Jung-Mi,Park, Jin-Ah,Park, Seung-Moon,Yang, Moon-Sik,Kim, Dae-Hyuk Korean Society for Molecular and Cellular Biology 2010 Molecules and cells Vol.30 No.3
A DnaJ-like gene, Cpdj1, a molecular chaperone and regulator of Hsp70 in Cryphonectria parasitica, was characterized. The protein product of Cpdj1 gene consists of 379 amino acids with a predicted molecular mass of 40.6 kDa and a pI of 7.79. The deduced protein sequence revealed preservation of the conserved hall-mark J-region and exhibited high homology to all known DnaJ-like proteins. Disruption of the Cpdj1 gene resulted in slow growth and produced colonies characterized by retarded growth and a deep orange color. Accordingly, reduced virulence of the Cpdj1- mutant was observed. This reduced growth rate was magnified when the Cpdj1- mutant was cultured under heat-stress conditions. Reduced conidiation was also observed in the Cpdj1- mutant, indicating that Cpdj1 gene, although not essential for cell viability, is required for appropriate cellular processes including growth and sporulation. Northern analysis showed that Cpdj1 was constitutively expressed, and when the culture was subject to high temperature, a strong induction of the transcript was observed. No significant difference in the expression and induction pattern of Cpdj1 was observed between virus-free EP155/2 and virus-infected hypovirulent UEP1 strains. However, further severe defects in mycelia growth and conidiation were observed in the hypovirus-infected Cpdj1- mutant suggesting that the presence of Cpdj1 is required for mycelia growth and sporulation of the hypovirus-infected strain.
Jung-Gu Lim,Joong-Gi Lee,김정미,Jin-Ah Park,박승문,양문식,김대혁 한국분자세포생물학회 2010 Molecules and cells Vol.30 No.3
A DnaJ-like gene, Cpdj1, a molecular chaperone and regulator of Hsp70 in Cryphonectria parasitica, was characterized. The protein product of Cpdj1 gene consists of 379amino acids with a predicted molecular mass of 40.6 kDa and a pI of 7.79. The deduced protein sequence revealed preservation of the conserved hall-mark J-region and exhibited high homology to all known DnaJ-like proteins. Disruption of the Cpdj1 gene resulted in slow growth and produced colonies characterized by retarded growth and a deep orange color. Accordingly, reduced virulence of the Cpdj1-null mutant was observed. This reduced growth rate was magnified when the Cpdj1-null mutant was cultured under heat-stress conditions. Reduced conidiation was also observed in the Cpdj1-null mutant, indicating that Cpdj1 gene, although not essential for cell viability, is required for appropriate cellular processes including growth and sporulation. Northern analysis showed that Cpdj1 was constitutively expressed, and when the culture was subject to high temperature, a strong induction of the transcript was observed. No significant difference in the expression and induction pattern of Cpdj1 was observed between virus-free EP155/2 and virus-infected hypovirulent UEP1 strains. However, further severe defects in mycelia growth and conidiation were observed in the hypovirus-infected Cpdj1-null mutant suggesting that the presence of Cpdj1 is required for mycelia growth and sporulation of the hypovirus-infected strain
임경재 ( Lim Kyoung-jae ),최예환 ( Engel Bernard A ),최중대 ( Choi Ye-hwan ),김기성 ( Choi Joong-dae ),신용철 ( Kim Ki-sung ),허성구 ( Shin Yong-cheol ),류창원 ( Heo Sung-gu ),( Lyou Chang Won ) 한국농공학회 2005 한국농공학회 학술대회초록집 Vol.2005 No.-
Accelerated soil erosion is a worldwide problem because of its economic and environmental impacts. To effectively estimate soil erosion and to establish soil erosion management plans, many computer models have been developed and used. The Revised Universal Soil Loss Equation (RUSLE) has been used in many countries, and input parameter data for RUSLE have been well established over the years. However, the RUSLE cannot be used to estimate the sediment yield for a watershed. Thus, the GIS-based Sediment Assessment Tool for Effective Erosion Control (SATEEC) was developed to estimate soil loss and sediment yield for any location within a watershed using the RUSLE and a spatially distributed sediment delivery ratio. SATEEC was enhanced in this study by developing new modules to:1) simulate the effects of sediment retention basins on the receiving water bodies, 2) prepare input parameters for the Web-based sediment decision support system using a GIS interface. This easy-to-operate SATEEC system can be used to identify areas vulnerable to soil loss and to develop efficient soil erosion management plans.
임중수(Lim Joong-Soo),정철구(Jung Chul-Gu),채규수(Chae Gyoo-Soo),박영철(Park Young Chul) 한국산학기술학회 2005 한국산학기술학회 학술대회 Vol.- No.-
본 논문에서는 고각이 변경되는 레이다의 탐지거리에 대해서 연구하였다. 일반적으로 레이다의 탐지거리는 지구의 곡율에 의한 지형적 영향과 전파의 감쇄로 인한 전력밀도에 의해서 결정되는 요소가 있다. 레이다의 탐지거리의 앞의 두 가지가 모두 만족되어야하며, 레이다의 고각이 변경되면 지형에 의한 탐지거리가 변경되므로 전체적인 탐지 거리에 변화가 생긴다. 본 논문에서는 레이다의 고각의 변경에 따른 가시거리 변경을 중점적으로 기술하였다.
임중수(Lim Joong-Soo),채규수(Chae Gyoo-Soo),정철구(Jung Chul-Gu),최창민(Choi Chang-Min) 한국산학기술학회 2005 한국산학기술학회 학술대회 Vol.- No.-
반도체 기술의 발달과 더불어 디지털 신호 기억회로는 매우 빠른 속도로 발달되었다. 그러나 수 GHz 이상의 높은 고주파 신호를 저장하였다가 복재하는 것은 매우 어려운 기술이다. 고주파 신호의 복재를 위해서 과거에는 신호 감쇄가 적은 고주파 케이블을 일정한 길이로 감아서 시간지연을 시키는 아날로그 방식의 고주파 신호 저장회로가 사용되었으나, 신호 샘플링 및 광대역 신호 변환기 등이 발전 되면서 고주파 신호를 고속으로 샘플링 한 뒤 디지털 회로에 저장 한 후에 필요시 고주파 신호를 복재하는 기술이 가능해 졌다. 본 논문에서는 주파수 대역이 3 옥타브 이상 되는 광대역 고주파 신호를 저장하고 복재할 때 샘플링 주파수에 따른 특성을 분석하였다.