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      • KCI등재

        Genome-wide identification and expression analysis of YTH domaincontaining RNA-binding protein family in cucumber (Cucumis sativus)

        Yong Zhou,Lifang Hu,Lunwei Jiang,Shiqiang Liu 한국유전학회 2018 Genes & Genomics Vol.40 No.6

        YTH domain-containing RNA-binding proteins are involved in post-transcriptional regulation and play important roles in the growth and development as well as abiotic stress responses of plants. However, YTH genes have not been previously studied in cucumber (Cucumis sativus). In this study, a total of five YTH genes (CsYTH1–CsYTH5) were identified in cucumber, which could be mapped on three out of the seven cucumber chromosomes. All CsYTH proteins had highly conserved C-terminal YTH domains, and two of them (CsYTH1 and CsYTH4) harbored extra CCCH and P/Q/N-rich domains. The phylogenesis, conserved motifs and exon–intron structure of YTH genes from cucumber, Arabidopsis and rice were also analyzed. The phylogenetically closely clustered YTHs shared similar gene structures and conserved motifs. An analysis of the cis-acting regulatory elements in the upstream region of these genes resulted in the identification of many cis-elements related to stress, hormone and development. Expression analysis based on the transcriptome data showed that some CsYTHs had development- or tissue-specific expression. In addition, their expression levels were altered under various stresses such as salt, drought, cold, and abscisic acid (ABA) treatments. These findings lay the foundation for the functional analysis of CsYTHs in the future.

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        In silico identification of potential drug targets in swine pathogen Haemophilus parasuis

        Zhuofei Xu,Lifang Ma,Huanchun Chen,Rui Zhou 한국유전학회 2012 Genes & Genomics Vol.34 No.2

        Gram-negative bacterium Haemophilus parasuis has recently become one of the most important etiological agents causing serious systemic disease (Glässer’s disease) in pigs. Antibiotic therapy has played a crucial role in the treatment of this disease. Antibiotic resistance observed from the clinical isolates of this pathogen urges us to discover novel drug targets for antimicrobial agents. In this study, we used a combined strategy including exploration of the gene essentiality and comparison of metabolic pathways to infer drug targets of H. parasuis. We identified 931 gene products essential for bacterial growth according to the DEG database. One hundred and ninety-nine enzyme-coding genes were found in the genome of H. parasuis but were absent in that of the swine host. Lastly,we determined 117 enzymes exhibiting essentiality and specificity to H. parasuis as a candidate set of drug targets. Comparison of metabolic pathways between the pathogen and host showed that 25 targeting enzymes belonged to nine unique pathways of the pathogen. The profile of promising targets identified in our study will provide a useful basis for developing more effective antibiotics against the severe swine disease caused by H. parasuis.

      • KCI등재

        Robust Three-step facial landmark localization under the complicated condition via ASM and POEM

        ( Weisheng Li ),( Lai Peng ),( Lifang Zhou ) 한국인터넷정보학회 2015 KSII Transactions on Internet and Information Syst Vol.9 No.9

        To avoid influences caused by pose, illumination and facial expression variations, we propose a robust three-step algorithm based on ASM and POEM for facial landmark localization. Firstly, Model Selection Factor is utilized to achieve a pose-free initialized shape. Then, we use the global shape model of ASM to describe the whole face and the texture model POEM to adjust the position of each landmark. Thirdly, a second localization is presented to discriminatively refine the subtle shape variation for some organs and contours. Experiments are conducted in four main face datasets, and the results demonstrate that the proposed method accurately localizes facial landmarks and outperforms other state-of-the-art methods.

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        Robust human tracking via key face information

        ( Weisheng Li ),( Xinyi Li ),( Lifang Zhou ) 한국인터넷정보학회 2016 KSII Transactions on Internet and Information Syst Vol.10 No.10

        Tracking human body is an important problem in computer vision field. Tracking failures caused by occlusion can lead to wrong rectification of the target position. In this paper, a robust human tracking algorithm is proposed to address the problem of occlusion, rotation and improve the tracking accuracy. It is based on Tracking-Learning-Detection framework. The key auxiliary information is used in the framework which motivated by the fact that a tracking target is usually embedded in the context that provides useful information. First, face localization method is utilized to find key face location information. Second, the relative position relationship is established between the auxiliary information and the target location. With the relevant model, the key face information will get the current target position when a target has disappeared. Thus, the target can be stably tracked even when it is partially or fully occluded. Experiments are conducted in various challenging videos. In conjunction with online update, the results demonstrate that the proposed method outperforms the traditional TLD algorithm, and it has a relatively better tracking performance than other state-of-the-art methods.

      • KCI등재

        Simultaneous regulation of photoabsorption and ferromagnetism of NaTaO3 by Fe doping

        Huan Yang,Liguo Zhang,Lifang Yu,Fang Wang,Zhenzhen Ma,Jie Zhou,Xiaohong Xu 한국물리학회 2018 Current Applied Physics Vol.18 No.11

        NaTa1-xFexO3 (0≤x≤0.40) nanocubes were synthesized by a relatively low temperature hydrothermal method, using Ta2O5, FeCl3 and NaOH as the precursors. The UV–vis diffuse reflectance spectra showed that NaTa1-xFexO3 had significant visible-light-absorbing capability, and the absorption edge of NaTaO3 shifted to longer wavelength with the increase of Fe dopants. Moreover, NaTa1-xFexO3 exhibited room-temperature ferromagnetism when Fe3+ occupied Ta5+ sites in NaTaO3 crystal lattice. The ferromagnetism is mainly attributed to the superexchange interactions between doped Fe3+, rather than the contribution of oxygen vacancies caused by Fe doping. Therefore, Fe doping can simultaneously regulate the optical and magnetic properties of NaTaO3 semiconductor, which will enable its potential applications in multifunctional optical-electronics and opticalspintronics devices.

      • KCI등재

        Vitamin D Supplementation is Beneficial for Children with Autism Spectrum Disorder: A Meta-analysis

        Liyao Song,Xiaomei Luo,Qing Jiang,Zhi Chen,Lifang Zhou,Dan Wang,Ai Chen 대한정신약물학회 2020 CLINICAL PSYCHOPHARMACOLOGY AND NEUROSCIENCE Vol.18 No.2

        Objective: We conducted a meta-analysis of randomized controlled trials to explore whether vitamin D supplementation is beneficial for symptom improvement in children with autism spectrum disorder. Methods: We systematically searched the PubMed database, EMBASE, Cochrane Library, Web of Science, Sino-Med, Wanfang Data, and China National Knowledge Infrastructure mainly up to September 2019. Using a fixed effects model, we calculated the standard mean difference with 95% confidence interval. Furthermore, we analyzed baseline serum 25-hydroxyvitamin D levels and outcome scores including the Social Responsiveness Scale and Child Autism Rating Scale scores after vitamin D supplementation. Results: There was no significant difference in baseline serum 25-hydroxyvitamin D levels among 203 children included from three studies in the meta-analysis. After vitamin D supplementation, the outcome scores in the experimental group were dramatically elevated compared with those in the control group (p = 0.03). Conclusion: Vitamin D supplementation improves the typical symptoms of autism spectrum disorder, as indicated by reduced Social Responsiveness Scale and Child Autism Rating Scale scores; thus, it is beneficial for children with autism spectrum disorder.

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