Gut Microbiota Alteration Influences Colorectal Cancer Metastasis to the Liver by Remodeling the Liver Immune Microenvironment

Yuan Na,Li Xiaoyan,Wang Meng,Zhang Zhilin,Qiao Lu,Gao Yamei,Xu Xinjian,Zhi Jie,Li Yang,Li Zhongxin,Jia Yitao 거트앤리버 소화기연관학회협의회 2022 Gut and Liver Vol.16 No.4

Background/Aims:This study aimed to explore the effect of gut microbiota-regulated Kupffer cells (KCs) on colorectal cancer (CRC) liver metastasis. Methods: A series of in vivo and in vitro researches were showed to demonstrate the gut microbiota and its possible mechanism in CRC liver metastasis. Results: Fewer liver metastases were identified in the ampicillin-streptomycin-colistin and colistin groups. Increased proportions of Parabacteroides goldsteinii, Bacteroides vulgatus, Bacteroides thetaiotaomicron, and Bacteroides uniformis were observed in the colistin group. The significant expansion of KCs was identified in the ampicillin-streptomycin-colistin and colistin groups. B. vulgatus levels were positively correlated with KC levels. More liver metastases were observed in the vancomycin group. An increased abundance of Parabacteroides distasonis and Proteus mirabilis and an obvious reduction of KCs were noted in the vancomycin group. P. mirabilis levels were negatively related to KC levels. The number of liver metastatic nodules was increased in the P. mirabilis group and decreased in the B. vulgatus group. The number of KCs decreased in the P. mirabilis group and increased in the B. vulgatus group. In vitro, as P. mirabilis or B. vulgatus doses increased, there was an opposite effect on KC proliferation in dose- and time-dependent manners. P. mirabilis induced CT26 cell migration by controlling KC proliferation, whereas B. vulgatus prevented this migration. Conclusions: An increased abundance of P. mirabilis and decreased amount of B. vulgatus play key roles in CRC liver metastasis, which might be related to KC reductions in the liver.

Finite-time Fault Detection Filtering for Switched Singular Systems with All Modes Unstable: an ADT Approach

Qingyu Su,Zhongxin Fan,Dezhen Zhang,Jian Li 제어·로봇·시스템학회 2019 International Journal of Control, Automation, and Vol.17 No.8

This paper finishes the fault detection (FD) problem for switched singular systems with all subsystems unstable. Considering the particularity of singular systems, the regularity and impulse-free property analysis on it are carried out. Utilizing a time-driven switching strategy, a switching signal is proposed and the finite-time stability is guaranteed firstly. Then, with the help of discretized Lyapunov function, a piecewise Lyapunov function is employed to accomplish the finite-time boundedness with L2-gain analysis. Through transferring the fault detection filters design into the L2-gain analysis, we can obtain the existence conditions of the FD detector via linear matrices inequalities (LMIs). Finally, the effectiveness and validity are illustrated by two examples.

<i>In Situ</i> Observation and Electrochemical Study of Encapsulated Sulfur Nanoparticles by MoS₂ Flakes

Tang, Wei,Chen, Zhongxin,Tian, Bingbing,Lee, Hyun-Wook,Zhao, Xiaoxu,Fan, Xiaofeng,Fan, Yanchen,Leng, Kai,Peng, Chengxin,Kim, Min-Ho,Li, Meng,Lin, Ming,Su, Jie,Chen, Jianyi,Jeong, Hu Young,Yin, Xuesong American Chemical Society 2017 JOURNAL OF THE AMERICAN CHEMICAL SOCIETY - Vol.139 No.29

<P>Sulfur is an attractive cathode material for next-generation lithium batteries due to its high theoretical capacity and low cost. However, dissolution of its lithiated product (lithium polysulfides) into the electrolyte limits the practical application of lithium sulfur batteries. Here we demonstrate that sulfur particles can be hermetically encapsulated by leveraging on the unique properties of two-dimensional materials such as molybdenum disulfide (MoS<SUB>2</SUB>). The high flexibility and strong van der Waals force in MoS<SUB>2</SUB> nanoflakes allows effective encapsulation of the sulfur particles and prevent its sublimation during <I>in situ</I> TEM studies. We observe that the lithium diffusivities in the encapsulated sulfur particles are in the order of 10<SUP>–17</SUP> m<SUP>2</SUP> s<SUP>–1</SUP>. Composite electrodes made from the MoS<SUB>2</SUB>-encapsulated sulfur spheres show outstanding electrochemical performance, with an initial capacity of 1660 mAh g<SUP>–1</SUP> and long cycle life of more than 1000 cycles.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jacsat/2017/jacsat.2017.139.issue-29/jacs.7b05371/production/images/medium/ja-2017-05371c_0006.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/ja7b05371'>ACS Electronic Supporting Info</A></P>

Underwater superoleophobic copper mesh coated with block nano protrusion hierarchical structure for efficient oil/water separation

Xinyu Qi,Zhuwei Gao,Chengxin Li,Shihao Wang,Xin Zou,Lan He,Zhongxin Liu 한국공업화학회 2023 Journal of Industrial and Engineering Chemistry Vol.119 No.-

Compared with monolayer nanosheet membranes, multilayer nanosheet membranes have low energyconsumption and can be mass-produced for industrial applications. Herein, a multi-layer nanostructuredmembrane, nickel-doped brochantite/Cu(OH)2 nano protrusion structure coated copper meshes(NBCPCMs), were successfully prepared. Firstly, the surface of the copper mesh was etched by chemicaloxidation to form a disordered copper hydroxide nanosheet layer. Secondly, the etched copper mesh surfacewas coated with nickel-controlled brochantite block-like protrusions by hydrothermal synthesismethod, and nickel-doped brochantite/Cu(OH)2 nanosheets were generated by high pressure. The resultsshowed that NBCPCM-4 had excellent hydrophilic/underwater superoleophobicity, the oil–water separationefficiency of immiscible cyclohexane/water mixture was the highest, about 98.54%, and still had avery high flux (>60 (kL/(hm2))) after multiple cycles of separation. In addition to ultra-high separationefficiency, NBCPCM-4 also had super durability. The copper mesh maintained good underwater superoleophobicityin different aqueous solutions. Therefore, NBCPCM-4 is a potential high-efficiency and sustainableoil–water separation membrane material. It is expected that the developed technology is usefulfor sea transportation and marine environment management, along with other industries.

Cisplatin-induced PANDAR-Chemo-EVs contribute to a more aggressive and chemoresistant ovarian cancer phenotype through the SRSF9-SIRT4/ SIRT6 axis

Hao Wang,Yinuo Liu,Yanan Wang,Xiumin Shang,Zhongxin Yan,Shengli Li,Wei Bao 대한부인종양학회 2024 Journal of Gynecologic Oncology Vol.35 No.2

Objective: We previously elucidated that long non-coding RNA Promoter of CDKN1A AntisenseDNA damage Activated RNA (PANDAR) as a p53-dependent oncogene to promote cisplatinresistance in ovarian cancer (OC). Intriguingly, high level of p53-independent PANDAR wasfound in cisplatin-resistant patients with p53 mutation. Here, our study probed the new rolesand the underlying mechanisms of PANDAR in p53-mutant OC cisplatin-resistance. Methods: A2780 and A2780-DDP cells were ser ved as OC cisplatin-sensitive and cisplatin-resistant cells. HO-8910PM cells were subjected to construct chemotherapy-inducedextracellular vesicles (Chemo-EVs). Transmission electron microscopy (TEM) and nanoparticletracking analysis were employed to evaluate Chemo-EVs. Cell viability was assessed using cellcounting kit-8 and colony formation assays. Cell apoptosis was assessed using Annexin V andpropidium iodide staining. The relationships between PANDAR, serine and arginine-rich pre-mRNA splicing factor 9 (SRSF9) were verified by RNA immunoprecipitation and fluorescencein situ hybridization. Tumor xenograft experiment was employed to evaluate the effects ofPANDAR-Chemo-EVs on OC cisplatin-resistance in vivo. Immunofluorescent staining andimmunohistochemistr y were performed in tumor tissue. Results: PANDAR level increased in OC patients with p53-mutation. PANDAR efflux enactedvia exosomes under cisplatin conditions. Additionally, exosomes from OC cell lines carriedPANDAR, which significantly increased cell sur vival and chemoresistance in vitro and tumorprogression and metastasis in vivo. During cisplatin-induced stress, SRSF9 was recruited tonuclear bodies by increased PANDAR and muted apoptosis in response to cisplatin. Besides,SRSF9 significantly increased the ratio of SIRT4/SIRT6 mRNA in OC. Conclusion: Cisplatin-induced exosomes transfer PANDAR and lead to a rapid adaptation ofOC cell sur vival through accumulating SRSF9 following cisplatin stress exposure.