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Lee, Jeong-Ha,Eom, Sung-Hwan,Lee, Eun-Hye,Jung, Yeoun-Joong,Kim, Hyo-Jung,Jo, Mi-Ra,Son, Kwang-Tae,Lee, Hee-Jung,Kim, Ji Hoe,Lee, Myung-Suk,Kim, Young-Mog The Korean Society of Phycology 2014 ALGAE Vol.29 No.1
To develop effective and safe acne vulgaris therapies with a continuing demand for new solutions, we investigated unique efficacy of an antibacterial agent from marine brown alga Eisenia bicyclis in treating acne vulgaris. The methanolic extract of E. bicyclis exhibited potential antibacterial activity against acne-related bacteria. The ethyl acetate fraction showed the strongest antibacterial activity against the bacteria among solvent fractions. Six compounds (1-6), previously isolated from the ethyl acetate fraction of E. bicyclis, were evaluated for antibacterial activity against acne-related bacteria. Among them, compound 2 (fucofuroeckol-A [FF]) exhibited the highest antibacterial activity against acne-related bacteria with a minimum inhibitory concentration (MIC) ranging from 32 to $128{\mu}g\;mL^{-1}$. Furthermore, FF clearly reversed the high-level erythromycin and lincomycin resistance of Propionibacterium acnes. The MIC values of erythromycin against P. acnes were dramatically reduced from 2,048 to $1.0{\mu}g\;mL^{-1}$ in combination with MIC of FF ($64{\mu}g\;mL^{-1}$). The fractional inhibitory concentration indices of erythromycin and lincomycin were measured from 0.500 to 0.751 in combination with 32 or $64{\mu}g\;mL^{-1}$ of FF against all tested P. acnes strains, suggesting that FF-erythromycin and FF-lincomycin combinations exert a weak synergistic effect against P. acnes. The results of this study suggest that the compounds derived from E. bicyclis can be a potential source of natural antibacterial agents and a pharmaceutical component against acnerelated bacteria.
( Sung-hwan Eom ),( Shin-kook Kang ),( Dae-sung Lee ),( Jeong-in Myeong ),( Jinhwan Lee ),( Hyun-woo Kim ),( Kyoung-ho Kim ),( Jae-young Je ),( Won-kyo Jung ),( Young-mog Kim ) 한국미생물 · 생명공학회 2016 Journal of microbiology and biotechnology Vol.26 No.4
We evaluated the synergistic antibacterial effect in combination with the chitosan-ferulic acid conjugate (CFA) and β-lactam antibiotics, such as ampicillin, penicillin, and oxacillin, against methicillin-resistant Staphylococcus aureus (MRSA) using fractional inhibitory concentration (FIC) indices. CFA clearly reversed the antibacterial activity of ampicillin, penicillin, and oxacillin against MRSA in the combination mode. Among these antibiotics, the combination of oxacillin-CFA resulted in a ΣFICmin range of 0.250 and ΣFICmax of 0.563, suggesting that the oxacillin-CFA combination resulted in an antibacterial synergy effect against MRSA. In addition, we determined that CFA inhibited the mRNA expression of gene mecA and the production of PBP2a, which is a key determinant for β-lactam antibiotic resistance, in a dosedependent manner. Thus, the results obtained in this study supported the idea on the antibacterial action mechanism that oxacillin will restore the antibacterial activity against MRSA through the suppression of PBP2a production by CFA.
( Sung Hwan Eom ),( Soo Jin Heo ),( Dae Sung Lee ),( Myung Suk Lee ),( Young Man Kim ),( Won Kyo Jung ),( Young Mog Kim ) 한국응용생명화학회(구 한국농화학회) 2011 Applied Biological Chemistry (Appl Biol Chem) Vol.54 No.3
Poncirus trifoliata was evaluated for antioxidant potential by several radical-scavenging assays, including 1,1-diphenyl-2-picrylhydrazyl, superoxide, hydroxyl, and alkyl radicals, using electron spin resonance technique. P. trifoliata extracts were prepared by using water and methanol, partitioning with different organic solvents. Among the tested samples, water extract and dichloromethane fraction evidenced relatively higher scavenging activities than those of other samples on reactive radicals and have high amount of antioxidant component such as flavonoids (8.5±0.3 and 8.3±0.2 mg rutin equivalents/g dry weight basis) and proanthocyanidin (2.6±0.3 and 2.8±0.5 mg catechin equivalents/g dry weight basis), respectively. Moreover, the water extract showed significantly high enhancement of cell viability against hydrogen peroxide-induced oxidative damage as well as reduction in the hydrogen peroxide-induced intracellular reactive oxygen species on CCL 13 cell line. These results indicate that P. trifoliate exerts various antioxidant effects against several reactive radicals and hydrogen peroxide-mediated cell damage. Thus, P. trifoliate could be a potential therapeutic modality for the treatment or prevention of several diseases associated with oxidative stress.
The Representation of the Neolithic Rock Art of Bangu-dae, South Korea
Lee, Sang-Mog Korea Association For Quaternary Research 2004 제사기학회지 Vol.18 No.2
The rock engraving of the Korea are dated by a very few direct archaeological contexts by linking their images to objects of known prehistoric date-although many of the figures are 'abstract'. This paper focuses on the rock art of Bangu-dae, located in the south-east of Korea. I try to the date of engravings from their contexts and the ways in which they can be studied.
( Sung Hwan Eom ),( Young Mi Kang ),( Jae Hong Park ),( Dae Ung Yu ),( Eun Tak Jeong ),( Myung Suk Lee ),( Young Mog Kim ) 한국수산과학회(구 한국수산학회) 2011 Fisheries and Aquatic Sciences Vol.14 No.3
The objective of this study was to select an effective microbial strain to improve the functional qualities of Eisenia bicyclis water extract by fermentation. For this purpose, several microorganisms isolated from traditional Korean fermented foods were inoculated and cultivated in E. bicyclis water extract. Ultimately, yeast strain YM-1 was selected for further study based on its total phenolic compound (TP) content and antioxidant activity, which were enhanced by microbial fermentation. The extract fermented by YM-1 exhibited a superior TP content and 2,2′-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity compared to extracts fermented by other microbes. The highest TP content and DPPH radical scavenging activity were observed after one day of YM-1 fermentation. Yeast strain YM-1 was identified as Candida utilis based on an analysis of its physiological characteristics. During fermentation of the extract by C. utilis YM-1, no significant difference was observed in the proximate composition, including moisture, crude lipid, crude protein, and crude ash. Fermentation by C. utilis YM-1 resulted in enhanced biological activity, including increases in the TP content and antioxidant activity. Thus, fermentation by C. utilis YM-1 is an attractive strategy for developing value-added food ingredients.
Lee, Su-Kyung,Kim, Jung Min,Lee, Mi-Young,Son, Kwang-Hee,Yeom, Young Il,Kim, Cheol-Hee,Shin, Youseung,Koh, Jong Sung,Han, Dong Cho,Kwon, Byoung-Mog Wiley Subscription Services, Inc., A Wiley Company 2006 International journal of cancer: Journal internati Vol.118 No.9
<P>As farnesylation of the Ras protein by farnesyl transferase is a critical step for the Ras functional activity, the farnesyl transferase inhibitor could affect H-Ras functions and the inhibitors such as arteminolide, SCH66336 and LB42908 completely inhibited Ras-farnesylation. However, they did not induce apoptosis of H-Ras-transformed cells with concentration for blocking H-Ras farnesylation. To determine the antitumor effects of the inhibitors, it was analyzed through the expression profile of genes, regulated by activated H-Ras or FTIs by using cDNA microarray. On the basis of the results, the relationship between H-Ras and MMPs expression was confirmed by RT-PCR, Western bolt, zymographic analysis and angiogenesis assay. Our results suggested that activation of MMP-13 as well as MMP-9 induced by H-Ras is involved in angiogenesis and with farnesyl transferase inhibitors, in part, exerts their anticancer effects. We confirmed that MMP-13 is a critical H-Ras target gene through chemical genomic approaches with farnesyl transferase inhibitors. © 2005 Wiley-Liss, Inc.</P>
Lee, Yun-Kyoung,Eom, Sung-Hwan,Hwang, Hye-Jin,Lim, Keun-Sik,Yang, Ji-Young,Chung, Yong-Hyun,Kim, Dong-Myung,Lee, Myung-Suk,Rhee, In-Koo,Kim, Young-Mog The Korean Society for Applied Biological Chemistr 2009 Applied Biological Chemistry (Appl Biol Chem) Vol.52 No.3
A gene encoding Catechol 2,3-dioxygenase (CD-2,3) was cloned from 3,4-dichloroaniline degrading bacterium Pseudomonas sp. KB35B. Comparison of the deduced amino acids of the CD-2,3 with other CD-2,3 proteins revealed that only three amino acids (Q116, N142 and V215) were particularly different in the CD-2,3 of KB35B. To elucidate how these differences are related with the biochemical properties of the protein, these amino acids were converted into the corresponding residue of other CD-2,3 proteins or Gly, respectively. The effects of amino acid conversion on the catalytic properties of the altered enzymes were then determined, and the results showed that Q116 is a critical amino acid involved in both substrate affinity and catalysis since the conversion of Q116 into other amino acids resulted in the change of both $K_m$ and $V_{max}$. However, the substitution of N142 showed only change of $V_{max}$, suggesting that this domain may involve in substrate catalysis. The mutation of V215 domain led to the deficiency of expression, implying that this amino acid is essential for the expression of the CD-2,3.
Lee, Sang-Seop,Lee, Kyung-Im,Yoo, Ji-Yun,Jeong, Moon-Jin,Park, Young-Mee,Kwon, Byoung-Mog,Bae, Yun-Soo,Han, Mi-Young Korean Society for Biochemistry and Molecular Biol 2001 Journal of biochemistry and molecular biology Vol.34 No.6
In the present study, an in vitro ELISA system to assess the interaction between Src homology (SH)2 domains and phosphotyrosine that contain peptides was established using purified GST-conjugated SH2 proteins and synthetic biotinylated phosphotyrosine that contain oligopeptides. The SH2 domains bound the relevant phosphopeptides that were immobilized in the streptavidin-coated microtiter plate in a highly specific and dose-dependent manner. The epidermal growth factor receptor (EGFR)-, T antigen (T Ag)-, and platelet-derived growth factor receptor (PDGFR)-derived phosphopeptides interacted with the growth factor receptor binding protein (Grb)2/SH2, Lck/SH2, and phosphatidyl inositol 3-kinase (PI3K) p85/SH2, respectively. No cross-reactions were observed. Competitive inhibition experiments showed that a short phosphopeptide of only four amino acids was long enough to determine the binding specificity. Optimal concentrations of the GST-SH2 fusion protein and phosphopeptide in this new ELISA system for screening the binding blockers were chosen at 2nM and 500nM, respectively. When two candidate compounds were tested in our ELISA system, they specifically inhibited the Lck/SH2 and/or p85/SH2 binding to the relevant phosphopeptides. Our results indicate that this ELISA system could be used as an easy screening method for the discovery of specific binding blockers of protein-protein interactions via SH2 domains.