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      • Rotavirus 전기영동형별을 위한 RNA 추출과정 및 조건의 비교연구

        조은경,김은순,양재명,김경희,조양자 한양대학교 의과대학 1992 한양의대 학술지 Vol.12 No.2

        Rotaviruses (Rv) are the major cause of severe dehydrating dearrhea among young children in Korea, where they are frequently responsible for 68% of the cases requiring hospitalization. Laboratory diagnosis of rotaviral infection in Korea depends mainly on enzyme-linked immunosorbent assay (ELISA) that employs expensive commercial diagnostic kits purchased from abroad and that shous the presence or absence of group A Rv only. PACE have however provided epidemiologic evidence to demonstrate the sxistence of different strains (both group A and atypical Rv) during diarrgeal outbreak, the appearance of new strains and desappearance of old ones from a community, shifts in the prenalence of strains, and the persistance of a single strain in newborns. For such reasons, we have felt that it is essential to raise the sensitivity and shorten the processing time for PAGE. We therfore have devised a rapid method for Rv RNA extration by directly extracting the Rv RNA from the specimens (that is, skipping freon extraction and running as ultracectrifuge) and using an urea-containing dissociation mixture to dissolve dried RNA for PAGE. We have also shorten the PAGE diagnostic results by sequential staining of the same gel with ethidium bromide and silver stain. With the use of the rapid method, it was feasible to increase the efficacy of the PAGE with reduced cost and working time. Phenol extration of stools, however, was essential in maintaining the sensitivity of the method.

      • KCI등재
      • 실증연구를 위한 주택용 태양열 온수기 모니터링 시스템 개발

        양동조,김재열,한재호,송경석,김우진 한국공작기계학회 2004 한국공작기계학회 추계학술대회논문집 Vol.2004 No.-

        The application of solar energy, in the field of alternative energy, was on the increase tendency. In the case of advanced nations, through continuous R&D, solar hot water heater with high efficiency has been used for the house and the industrial process on business, advanced nations were reached up the experimental stage of solar generation system. But, the actual circumstance of the domestic has been not accomplished the popularization of solar hot water heater and the settlement of it which is the fundamental stage of the solar energy usage. This trouble, the domestic was flooded with small enterprise for producing solar hot water heater, was caused by the popularization and the production without verification of performance. To supply the monitoring program for evaluating solar hot water heater, this research was purpose to improve the technical development of the enterprise for producing solar-heat hot-water-boiler and served as an aid for the enlargement and the popularization on solar energy.

      • 비타민 A 섭취가 에탄올을 급여한 흰쥐의 지질함량과 항산화 영양소 상태에 미치는 영향

        양경미,서정숙 경산대학교 생명자원개발연구소 2002 생명자원과 산업 Vol.6 No.-

        본 연구는 에탄올의 공급과 함께 비타민 A 활성물질을 식이 중에 보충하여 흰쥐에게 공급한 후 체내 지질 함량과 항산화영양소의 변화를 관찰하고자 시도되었다. 각 실험군은 에탄올을 rq여하고 동시에 비타민 A를 결핍시킨 실험군(FE), 에탄올과 함께 β-carotene 급여군(BE), 에탄올과 retinyl acetate 급여군(RE), 13-cis-retinoic acid 급여군(RAE) 그리고 에탄올 대신에 동일한 열량을 설탕으로 공급시킨 각각의 pair-fed 군(FP, BP, RP, RAP)으로 구분하여 각각 8마리씩 임의배치하여 7주간 액체식이 형태로 사육하였다. 혈장 내 총 지질 함량은 비타민 결핍군에서 가장 높았으며 에탄올 급여군 중 pair-fed 군에 비해서는 RAE군 만이 유의적인 증가를 보였다. 중성지질 함량은 RAE와 RAP군에서 가장 높았으며 에탄올 급여군 중 pair-fed 군에 비해 RE군만이 유의적인 증가를 보였고 인지질은 에탄올과 비타민 A 급여에 의한 영향은 없었다. 총 콜레스테롤 함량은 에탄올 급여군 중 pair-fed 군에 비해 RE군 만이 유의적인 증가를 보였다. HDL-콜레스테롤은 에탄올 급여나 비타민 A의 섭취형태에 의한 차이가 크게 나타나지 않았으며, HDL-콜레스테롤/총 콜레스테롤은 에탄올 급여군 중 BE군 만이 pair-fed 군에 비해 유의적으로 낮았다. 이와같이 혈장과 비타민 A 결핍에 의해서 가장 많은 영향을 받았으며, 이 영향은 비타민 A의 보충에 의해서도 조절될 수 없었다. 혈장내 α-tocopherol 함량은 에탄올 급여로 감소되었으나 간 조직내에서는 β-carotene 섭취군만이 낮은 함량을 보였다. 그러나 간 조직내 tocopheryl acetate 함량은 pair-fed 군에 비해서 에탄올 급여로 현저히 저하되었다. 혈장과 간 조직내에서 에탄올 급여에 의한 비타민 E 함량 감소는 tetinyl acetate 공급으로 그 감소 정도가 가장 저하되었다. 혈장내 총 비타민 C 함량은 에탄올 급여와 동시에 β-carotene이나 retinyl acetate 섭취군이 pair-fed 군에 비해 낮았으며 환원형과 산화형 비타민 C 함량비는 에탄올 급여로 낮았으나 β-carotene을 섭취시킨 군만이 높게 나타났다. 간 조직내 총 비타민 C 함량은 13-cis-retinoic acid 섭취군을 제외한 전군에서 에탄올 급여로 증가되었으며 환원형과 산화형 비타민 C비는 에탄올 급여군 중 β-carotene을 섭취시켰을 때 가장 높았다. 이러한 결과들로 보아 에탄올은 체내 항산화 영양소 상태의 변화를 초래하며 비타민 A의 공급여부 뿐만 아니라 형태별 섭취에 의해서 항산화 영양소 상태가 변화될 수 있는 것으로 나타났다. 그러나 생체내 항산화 영양소들은 서로 상쇄, 상승 혹은 길항작용을 통해서 상호영향을 미치므로 각각의 항산화 영양소 상태 변화에 대한 정확한 기전규명을 위해서는 다각적인 연구가 요구된다고 볼 수 있다. 그러나 지질과산화물의 함량과 체내 항산화 영양소 상태는 상호관련성이 있었으며 본 실험에서 에탄올 공급에 의한 항산화 영양소 함량의 감소는 대체로 비타민 A를 retinyl acetate 형태로 섭취시켰을 때 다소 경감되는 것으로 나타났다. The present study was conducted to investigate the effect of dietary vitamin A on the status of lipid content and antioxidants in ethanol-treated rats. Weaning rats were fed a basal diet until they reached about 160-180g body weight. Thereafter, four experimental groups were fed a liquid diet containing 36% ethanol of tatal calorie and four pair-fed groups were fed isocaloric sucrose instead of ethanol. Additionally, the liquid diet contained adequate amount of β-carotene, retinyl acetate, or 13-cis-retinoic acid except vitamin A deficient diet. The rats were sacrificed after 7 weeks of feeding periods. Plasma contents of total lipid triglyceride were increased in vitamin A-deficient and 13-cis-retinoic acid groups. Plasma concentration of total cholesterol in retinyl acetate group was higher than that of pair-fed controls. HDL-cholesterol/total cholesterol was increased in β-carotene group. The hepatic levels of total lipid and triglyceride in β-carotene. The hepatic levels of total lipid and triglyceride in β-carotene and retinyl acetate groups were significantly increased compaired with the pair-fed control groups. Total cholesterol content was significantly increased in vitamin A-deficient group with ethanol. In the changes of antioxidants status, significant decrease in hepatic vitamin E content was found in rats treated with chronic ethanol. However, dietary supplementation of retinyl acetate modified the change to some extent. Total vitamin C content of liver increased in vitamin A-deficient or β-carotene groups with ethanol feeding.

      • 영아 천식의 임상적 고찰 : Asthma prospective score에 의한 Based on Asthma Prospective Score

        양은석,노영일,문경래,박영봉,박상기,변주남 조선대학교 부설 의학연구소 1999 The Medical Journal of Chosun University Vol.24 No.1

        A Clinical study was made on 40 patients for infantile asthma less than 2 years old who had been admitted to the Department of Pediatrics of Chosun University Hospital from January 1997 to December 1998. We evaluated family history of allergic diseases, clinical features, serum IgE level, eosinophilia, chest radiologic findings and response to bronchodilator. The results obtained were as follows ; 1) The male to female ratio of patients studied was 4 : 1. 2) Eighteen of the 40 patients (45.0%) had a family history of allergic diseases, of which 10 patients had maternal allergic diseases. 3) Atopic dermatitis was the most common disease (37.5%) among the associated allergic diseases. 4) Thirty two of the 40 patients (80.0%) showed high serum total 19E levels. 5) Only 5 of the 20 patients who were examined by MAST showed positive reaction. 6) The frequency of chest radiologic findings observed in these patients was as follows ; hyperinflation (52.5%), increased bronchovascular marking(30.0%), no pathologic findings(25.0%), and pneumonic infiltration(25.0%). 7) In response to bronchodilator 28 cases(70.0%) were effective. 8) When we divided the 40 patients into two groups by the diagnostic criteria of Asthma prospective score (AP score), 30 patients(75.0%) were included in a compatible group. On the basis of AP score, family history of major or minor allergic dieases and the responsiveness to bronchodilator were important factors for the diagnosis of asthma.

      • 경로 트리밍을 이용한 항공 라우팅용 공구경로 생성

        양경부,조경호 제주대학교 공과대학 첨단기술연구소 2007 尖端技術硏究所論文集 Vol.18 No.-

        An offset loop of profiles in sheet metal part is a tool paths for profile-machining, Offsetting is one of the most geometric problem in case of complex shape. In this paper we describe a method for generating NC tool paths for aerospace routing process using path trimming based on pair-wise intersection.

      • 신도시 아파트 거주가구의 근린환경 만족도 고찰

        양현정;신경주 한양대학교 2007 韓國 生活 科學 硏究 Vol.27 No.1

        The purpose of this study is to analyze differences in between neighborhood satisfaction of apartment residents in five Newtowns. To accomplish this aims, the 361 samples of the apartment residents were partially used from the que.stionnaire data surveyed for a study on the housing consumption behavior of the Metropolitan area by KRIHS (Korea Research Institute for Human Settlements) in 1999. To data analysis, descriptive statistics, t-test, one-way ANOVA, Duncan test, factor analysis, crosstabulation analysis, correlation and multiple regression analysis were performed with the SPSS Win program (Ver. 10. 0). Conclusions are as follows : 1. The apartment residents in five Newtowns were mainly in thirties and forties and in the level of a white-collar worker (office worker). The greater part of this sample possessed their own house and its size was large-sized with a room of four. 2. The present neighborhood satisfaction of the apartment residents in Newtowns was 3.56 points. The eight factors (Traffic facilities, Green space, Educational facilities, Neighborhood facilities, Cultural facilities, Participation condition, Financial condition and Public services) of neighborhood satisfaction were between 3 and 4 points. Household characteristics and housing characteristics did not have an significant effect on the neighborhood satisfaction. But the residential town had an effect on it. Residents in Bundang were satisfied much more than other towns (Chungdong, Ilsan, Pyongchon, Sanbon) with Traffic facilities, Green space, Neighborhood facilities, Cultural facilities, Participation condition and Financial condition. Bundang considered residents more than other Newtowns in six aspects. Residents in Chungdong were satisfied with Cultural facilities. Residents in Sanbon were satisfied with Traffic facilities, Educational facilities and Financial condition.

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