膀胱의 單臟器成 Shwartzman反應에 관한 超微形態學的 硏究

권민아,장세국,서인수,손태중 慶北大學校 醫科大學 1990 慶北醫大誌 Vol.31 No.4

본 연구는 감염성 방광염의 성립의 조건으로 Shwartzman반응의 기전이 참여할 것인가를 검토하고, 방광의 단장기성 Shwartzman반응에서 초기의 형태적 변화를 알아보기 위하여 시도하였다. 실험은 백색 암컷 가토를 실험동물로 하여 방광내에 내독소를 주입하고 24시간후에 정맥내로 같은 내독소를 주사하여 야기조작 15및 30분, 1, 2 및 4시간후에 방광을 적출하여 육안, 광학, 주사전자 및 투과 전자현미경으로 관찰하였던 바 그 성적을 요약하면 다음과 같다. 광학 현미경으로는 반응의 초기부터 표재세포가 호산성 덩어리로의 변성 및 괴사 탈락이 나타나고, 중간세포 및 기저세포는 야기조작 수시간 후부터 공포성 변성을 보이고, 같은 시기에 상피하 미소혈관은 울혈 및 주변 조직의 부종을 보이고, 호중구의 침윤도 관찰되었다. 주사 전자현미경으로는 표재세포 표면의 microridge의 blurring과 표면구조의 편평화 및 세포간 결합장치의 이완등을 초기에서 부터 볼 수 있었고, 수시간이 지난 후에는 군데군데에서 상피의 괴사 탈락이 관찰되었다. 투과 전자현미경으로는 포재세포의 apoptosis를 초기에서부터 볼 수 있었고, 수시간 후에는 상피층 전층의 괴사까지 관찰되었고, 기저세포에는 autophage가 증가된 것이 관찰되었다. 가장 특징적 변화는 상피직하 점막하층 미소혈관계의 변화로 반응의 초기에서부터 내피세포의 종창등의 상해성 변화를 보였고, 수시간후에는 혈소판의 응집과 호중구의 변연화 및 유주등을 관찰할 수 있었다. 이상의 성적으로 보아 방광염의 성립에 Shwartzman반응의 기전이 참여할 수 있는 것으로 생각되며, 방광의 단장기성 Shwartzman반응의 주된 반응을 하는 장소는 상피직하부의 미소혈관계인 것으로 사료된다. This study was carried out to investigate the possible role of Shwartzman reaction in the development of the cystitis and the morphologic changes in early stage of the reaction. Endotoxin extracted from E. coli was injecting same endotoxin via ear vein, 24 hours after first infection. Animals were sacrificed 15 and 30 minutes, and 1, 2 and 4 hours after the last injection. The Urinary bladders were extracted out and examined with the light, transmision electron, and scanning electron microscopes. The results obtained were summarized as follows: Ligth microscopy showed eosinophilic degradation, necrosis and detachment of the superficial cells within 1 hour after provocation treatment. Intermediate and basal cells also showed swelling after few hours. Subepithelial microvasculatures showed congestion with perivascular edema and neutrophilic infiltration from the early stages. Scanning electron microscopy dischlosed blurring of microridge structures on superficial cells with loosening of the intercellular junction from early stage. Necrosis and detachment of epithelial cells were noted in several areas after few hours, only remaining basement membranes. Transmission electron microscopy showed apoptosis of the superficial cells, with partial necrosis through total thickness of the epithelial layer after several hours. Many autophagic vacuoles were found in basal cells. Characteristic vascular change was noted in the microvasculatures on the submucosa, consisting of swelling and archade formation of the endothelial cells with platelet aggregation, and margination and emigration of neutrophils. The results suggest that Shwartzman reaction could be participated on the development of cystitis, and subepithelial small vessels were the main focus of the reaction.

전분으로부터 Lactobacillus manihotivorans에 의한 젖산생산시 최적발효조건

손민식,권윤중 경기대학교 기초과학연구소 2008 기초과학논문집 Vol.21 No.-

L(+)-lactic acid is a raw material for synthesis of polylactic acid (PLA) which is an essential biodegradable polymer, In the polymerization process, the stereospecificity of lactic acid is very important, and selective production of stereospecific lactic acid has been carried out by lactic acid bacteria (LAB). Refined glucose and sucrose are the most commonly used substrate for producing lactic acid in fermentation process. However, production cost can be reduced if starch is used, as the saccharification process can be eliminated. Amylolytic lactic acid bacteria (ALAB) are able to simultaneously hydrolyze and ferment starch to lactic acid. Among several ALAB, Lactobacillus manihotivorans LMG 18010 was reported as homofementor that produces only L(+)-lactic acid. In this study, the influence of the most important operational variables on lactic acid production were examined with flask culture. The optimum initial pH for direct fermentation of starch to L(+)-lactic acid by Lb. manihotivorans was 6.5. With 10%(v/v) of inoculum of 12 hr-old cells which was grown in starch containing medium, the best results in lactic acid production were obtained in shake-flask fermentations.

삼일열 말라리아에 합병된 비장 파열 1예

홍경욱,이정아,김원진,박철민,권혜리,박혜원,엄중식 대한감염학회 2007 감염과 화학요법 Vol.39 No.5

국내의 토착형 말라리아는 삼일열 말라리아로, 1993년부터 재유행이 시작되었다. 말라리아의 중요한 합병증으로 드물지만 비장 파열이 있는데, 이는 주로 삼일열 말라리아에 의해서 발생되며, 국내에서는 1999년 말라리아에 의한 비장 파열로 비장 절제술을 시행한 예가 보고된 바 있다. 현재까지는 비장 파열이 있을 때 비장 절제술이 일차적인 치료로 되어 있으나, 비장 절제술 후의 감염 위험성이 크므로, 활력 징후가 불안정하거나, 출혈이 지속되는 소견이 보이지 않으면 가급적 보존적 치료를 하는 것이 추천된다. 저자들은 비장 파열이 동반된 말라리아 환자에서 비장 절제술을 시행하지 않고 보존적 치료로 호전된 예를 경험하여 보고하는 바이다. Plasmodium vivax has been the predominant Plasmodium species in the Republic of Korea and reemerged in 1993. Spontaneous rupture of malarial spleen is a rare but important complication associated with P. vivax infection. Only one case of spontaneous rupture of malarial spleen was reported in Korea in 1999. Splenectomy is still accepted as the treatment of choice in cases of spontaneous rupture of the spleen. But, considering increased risk of postsplenectomy infecion, splenectomy should be reserved for those patients with severe rupture or those with continued or recurrent bleeding. And conservative treatment should be considered in selected, closely monitored patients. We report a patient with P. vivax malaria who developed a spontaneous splenic rupture and was improved by conservative treatment.

Behavior Analysis of Discharged DIC Concentrated Seawater through Towed Pipe Injection from Ship

Kwon, Seong-Min,Kim, Kang-Min,Song, Hyeon-Woo,Lee, Joong-Woo Korean Institute of Navigation and Port Research 2019 한국항해항만학회지 Vol.43 No.2

Climate change is a very vital issue that can be no longer avoided. Korea has been a top-level country Iin dealing with carbon dioxide emissions since 1960. Many studies have been conducted to suppress or eliminate carbon dioxide emissions, which account for a large portion of greenhouse gases. Carbon Capture and Storage (CCS), the most practical method of them, plays a significant role. However, these methods have the disadvantage of the limits of geographical distribution and high possibility of re-emission into the atmosphere. Recently, ocean storage has been studied using Accelerated Weathering of Limestone (AWL), a technique for storing carbon dioxide in the ocean as an alternative to CCS, an underground storage. AWL is a method of converting carbon dioxide into concentrated water in the form of bicarbonate ion and discharging it to the ocean to dilute and store it. It does not cause re-emission to the atmosphere, and the discharged concentrated water increases the alkalinity of the ocean to prevent marine acidification. The objective of this study was to understand the behavior of DIC (Dissolved Inorganic Carbon) including carbon dioxide during the ocean discharge of bicarbonate ion concentrated water in AWL method. This study area was set near Ulleung-do where sufficient water depth and operational efficiency were secured. CORMIX model was used to calculate the material diffusion by submerged discharge using ship.

Rapid and simple method for DNA extraction from plant and algal species suitable for PCR amplification using a chelating resin Chelex 100

HwangBo, Kwon,Son, Su-Hyun,Lee, Jong-Suk,Min, Sung-Ran,Ko, Suk-Min,Liu, Jang-R.,Choi, Dong-Su,Jeong, Won-Joong The Korean Society of Plant Biotechnology 2010 Plant biotechnology reports Vol.4 No.1

A DNA extraction method using Chelex 100 is widely used for bacteria, Chlamydomonas, and animal cell lines, but only rarely for plant materials due to the need for additional time-consuming and tedious steps. We have modified the Chelex 100 protocol and successfully developed a rapid and simple method of DNA extraction for efficient PCR-based detection of transgenes from a variety of transgenic plant and algal species. Our protocol consists of homogenizing plant tissue with a pestle, boiling the homogenized tissue in a microfuge tube with 5% Chelex 100 for 5 min, and centrifuging the boiled mixture. The supernatant, which is used for PCR analysis, was able to successfully amplify transgenes in transgenic tobacco, tomato, potato, Arabidopsis, rice, strawberry, Spirodela polyrhiza, Chlamydomonas, and Porphyra tenera. The entire DNA extraction procedure requires <15 min and is therefore comparable to that used for bacteria, Chlamydomonas, and animal cell lines.

Behavior Analysis of Discharged DIC Concentrated Seawater through Towed Pipe Injection from Ship

Seong-Min Kwon,Kang-Min Kim,Hyeon-Woo Song,Joong-Woo Lee 한국항해항만학회 2019 한국항해항만학회지 Vol.43 No.2

Climate change is a very vital issue that can be no longer avoided. Korea has been a top-level country Iin dealing with carbon dioxide emissions since 1960. Many studies have been conducted to suppress or eliminate carbon dioxide emissions, which account for a large portion of greenhouse gases. Carbon Capture and Storage (CCS), the most practical method of them, plays a significant role. However, these methods have the disadvantage of the limits of geographical distribution and high possibility of re-emission into the atmosphere. Recently, ocean storage has been studied using Accelerated Weathering of Limestone (AWL), a technique for storing carbon dioxide in the ocean as an alternative to CCS, an underground storage. AWL is a method of converting carbon dioxide into concentrated water in the form of bicarbonate ion and discharging it to the ocean to dilute and store it. It does not cause re-emission to the atmosphere, and the discharged concentrated water increases the alkalinity of the ocean to prevent marine acidification. The objective of this study was to understand the behavior of DIC (Dissolved Inorganic Carbon) including carbon dioxide during the ocean discharge of bicarbonate ion concentrated water in AWL method. This study area was set near Ulleung-do where sufficient water depth and operational efficiency were secured. CORMIX model was used to calculate the material diffusion by submerged discharge using ship.

Hierarchical Chitin Fibers with Aligned Nanofibrillar Architectures: A Nonwoven-Mat Separator for Lithium Metal Batteries

Kim, Joong-Kwon,Kim, Do Hyeong,Joo, Se Hun,Choi, Byeongwook,Cha, Aming,Kim, Kwang Min,Kwon, Tae-Hyuk,Kwak, Sang Kyu,Kang, Seok Ju,Jin, Jungho American Chemical Society 2017 ACS NANO Vol.11 No.6

<P>Here, we introduce regenerated fibers of chitin (Chiber), the second most abundant biopolymer after cellulose, and propose its utility as a nonwoven fiber separator for lithium metal batteries (LMBs) that exhibits an excellent electrolyte-uptaking capability and Li-dendrite mitigating performance. Chiber is produced by a centrifugal jet-spinning technique, which allows a simple and fast production of Chibers consisting of hierarchically aligned self-assembled chitin nanofibers. Following the scrutinization on the Chiber-Li-ion interaction via computational methods, we demonstrate the potential of Chiber as a nonwoven mat-type separator by monitoring it in Li-O-2 and Na-O-2 cells.</P>

갑상선 미세 유두암의 수술 전 초음파 검사와 세침흡입검사의 결과에 따른 수술범위 선택의 타당성 검토 : 예비 보고

권중근(Joong Keun Kwon),이상민(Sang Min Lee),이호민(Ho Min Lee),남정권(Jung Gwon Nam),이태훈(Tae-Hoon Lee),이종철(Jong Cheol Lee) 대한두경부종양학회 2011 대한두경부 종양학회지 Vol.27 No.1

Background and Objectives:Although it is well established that patients with papillary thyroid microcarcinoma (PTMC) have a highly favorable prognosis, the extent of thyroid surgery for PTMC remains unclear. According to the 2011 revised Korean Thyroid Association guideline, the choice of surgical strategy(total thyroidectomy versus lobectomy) for PTMC depends on solely preoperative diagnostic scrutinies-ultrasonography and fine needle aspiration cytology. We want to know how accurately these preoperative diagnostic scrutinies define the choice of surgical strategy for PTMC. Materials and Methods:For 119 patients who underwent total thyroid-ectomy with central neck dissection for PTMC, retrospectively, we compared the choice of surgery according to preoperative work up and postoperative pathologic findings. Results:Overall accuracy of the choice of sur-gery by preoperative work up was 61%. Among patients recommended lobectomy on preoperative work up, completion thyroidectomy on postoperative pathology might be necessary for 60% of patients and hidden central node metastasis was revealed in 31% of patients. Conclusions:The results of this study compel us to reinvestigate the current treatment guideline for PTMC. On current guideline according to the sonography and fine needle aspiration cytology, it might be thought to be better to choose more aggressive surgical strategy.

Carbon Nanotube/Magnesium Composite as a Hydrogen Source

Yu, Min Kyu,Oh, Se Kwon,Kim, Min Joong,Hwang, Jae Won,Yoon, Byoung Young,Kwon, Hyuk Sang American Scientific Publishers 2015 Journal of Nanoscience and Nanotechnology Vol.15 No.11

산불피해 소나무재의 목질펠릿으로의 이용가능성 평가

권성민 ( Sung Min Kwon ),조재현 ( Jae Hyun Cho ),이성재 ( Sung Jae Lee ),권구중 ( Gu Joong Kwon ),황병호 ( Byung Ho Hwang ),이귀현 ( Gwi Hyun Lee ),한규성 ( Gyu Seong Han ),차두송 ( Du Song Cha ),김남훈 ( Nam Hun Kim ) 한국목재공학회 2007 목재공학 Vol.35 No.4