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Kosar, Moradi,Mahmoud, Otroshy 한국작물학회 2012 Journal of crop science and biotechnology Vol.15 No.3
The present study describes the procedure for micropropagation of Dracocephalum kotschyi L. using shoot tips from in vitro-germinated plants. The best response was observed for shoot tips on MS medium containing 5 mg 6-benzylaminopurine $L^{-1}$ and 0.2 mg 1-naphthaleneacetic $L^{-1}$ acid. Regeneration for other types of the explant hypocotyls and cotyledons did not show satisfactory results so that the explants did not develop into normal shoots and in turn developed into the calli after 12 days of culture. Histochemical analysis showed that only the shoot tip revealed a direct induction of more teratological protuberances that arise around the cut end of the explants. Elongation of shoot buds was obtained on MS medium containing 1 mg BAP $L^{-1}$ + 0.5 mg IBA $L^{-1}$. Regenerated shoots rooted best on the same medium of elongation. After hardening, the rooted plants were transferred to the greenhouse where they grew, matured, and flowered normally with a survival rate of 95%. We concluded that the present protocol can be efficiently used for mass propagation of Dracocephalum kotschyi.
( Kosar A. Filiz ),( Dalar Levent ),( Eryuksel Emel ),( Uysal Pelin ),( Akbas Turkay ),( Kekecoglu Aybuke ) 대한결핵 및 호흡기학회 2016 Tuberculosis and Respiratory Diseases Vol.79 No.3
Background: One to three percent of cases of acute tuberculosis (TB) require monitoring in the intensive care unit (ICU). The purpose of this study is to establish and determine the mortality rate and discuss the causes of high mortality in these cases, and to evaluate the clinical and laboratory findings of TB patients admitted to the pulmonary ICU. Methods: The data of patients admitted to the ICU of Yedikule Chest Diseases and Chest Surgery Education and Research Hospital due to active TB were retrospectively evaluated. Demographic characteristics, medical history, and clinical and laboratory findings were evaluated. Results: Thirty-five TB patients (27 males) with a median age of 47 years were included, of whom 20 died within 30 days (57%). The Acute Physiology and Chronic Health Evaluation II (APACHE II) and Sequential Organ Failure Assessment (SOFA) scores were significantly higher, and albumin and PaO2/FIO2 levels were significantly lower, and shock, multiple organ failure, the need for invasive mechanical ventilation and drug resistance were more common in the patients who died. The mortality risk was 7.58 times higher in the patients requiring invasive mechanical ventilation. The SOFA score alone was a significant risk factor affecting survival. Conclusion: The survival rate is low in cases of tuberculosis treated in an ICU. The predictors of mortality include the requirement of invasive mechanical ventilation and multiple organ failure. Another factor specific to TB patients is the presence of drug resistance, which should be taken seriously in countries where there is a high incidence of the disease. Finding new variables that can be established with new prospective studies may help to decrease the high mortality rate.
Filiz, Kosar A.,Levent, Dalar,Emel, Eryuksel,Pelin, Uysal,Turkay, Akbas,Aybuke, Kekecoglu The Korean Academy of Tuberculosis and Respiratory 2016 Tuberculosis and Respiratory Diseases Vol.79 No.3
Background: One to three percent of cases of acute tuberculosis (TB) require monitoring in the intensive care unit (ICU). The purpose of this study is to establish and determine the mortality rate and discuss the causes of high mortality in these cases, and to evaluate the clinical and laboratory findings of TB patients admitted to the pulmonary ICU. Methods: The data of patients admitted to the ICU of Yedikule Chest Diseases and Chest Surgery Education and Research Hospital due to active TB were retrospectively evaluated. Demographic characteristics, medical history, and clinical and laboratory findings were evaluated. Results: Thirty-five TB patients (27 males) with a median age of 47 years were included, of whom 20 died within 30 days (57%). The Acute Physiology and Chronic Health Evaluation II (APACHE II) and Sequential Organ Failure Assessment (SOFA) scores were significantly higher, and albumin and $PaO_2/FIO_2$ levels were significantly lower, and shock, multiple organ failure, the need for invasive mechanical ventilation and drug resistance were more common in the patients who died. The mortality risk was 7.58 times higher in the patients requiring invasive mechanical ventilation. The SOFA score alone was a significant risk factor affecting survival. Conclusion: The survival rate is low in cases of tuberculosis treated in an ICU. The predictors of mortality include the requirement of invasive mechanical ventilation and multiple organ failure. Another factor specific to TB patients is the presence of drug resistance, which should be taken seriously in countries where there is a high incidence of the disease. Finding new variables that can be established with new prospective studies may help to decrease the high mortality rate.
Moradi Kosar,Otroshy Mahmoud 한국작물학회 2012 Journal of crop science and biotechnology Vol.15 No.3
The present study describes the procedure for micropropagation of Dracocephalum kotschyi L. using shoot tips from in vitro-germinated plants. The best response was observed for shoot tips on MS medium containing 5 mg 6- benzylaminopurine L-1 and 0.2 mg 1-naphthaleneacetic L-1 acid. Regeneration for other types of the explant hypocotyls and cotyledons did not show satisfactory results so that the explants did not develop into normal shoots and in turn developed into the calli after 12 days of culture. Histochemical analysis showed that only the shoot tip revealed a direct induction of more teratological protuberances that arise around the cut end of the explants. Elongation of shoot buds was obtained on MS medium containing 1 mg BAP L-1 + 0.5 mg IBA L-1. Regenerated shoots rooted best on the same medium of elongation. After hardening, the rooted plants were transferred to the greenhouse where they grew, matured, and flowered normally with a survival rate of 95%. We concluded that the present protocol can be efficiently used for mass propagation of Dracocephalum kotschyi.
Purunusides A-C, α-Glucosidase Inhibitory Homoisoflavone Glucosides from Prunus domestica
Shaheen Kosar,Itrat Fatima,Azhar Mahmood,Rehana Ahmed,Abdul Malik,Muhammad Iqbal Chouhdary,Sumaira Talib 대한약학회 2009 Archives of Pharmacal Research Vol.32 No.12
Purunusides A-C (1-3), new homoisoflavone glucosides together with the known compounds β-sitosterol (4) and 6,7-methylenedioxy-8-methoxycoumarin (5) have been isolated from nbutanol and ethyl acetate soluble fractions of Prunus domestica. Their structures were assigned on the basis of spectral studies. The compounds 1-3 showed potent inhibitory activity against the enzyme α-glucosidase.
Cavitating nozzle flows in micro- and minichannels under the effect of turbulence
Morteza Ghorbani,Mehmet Yildiz,Devrim Gozuacik,Ali Kosar 대한기계학회 2016 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.30 No.6
The cavitation phenomenon inside micro- and minichannel configurations was numerically investigated. The simulations for each channel were performed at different upstream pressures varying from 1 to 15 MPa. Two microchannel configurations with inner diameters of 152 and 254 μm and two minichannel configurations with inner diameters of 504 and 762 μm were simulated. To validate the numerical approach, micro-jet impingement from a microchannel with an inner diameter of 152 μm was first simulated at different Reynolds numbers. Then, the mixture model was used to model the multiphase flow inside the channels. The results of this study present major differences in the cavitating flows between the micro- and miniscale channels and show that the pressure profile and vapor phase distribution exhibit different features. The static pressure drops to negative values (tensile stress) in microchannels, while the minimum static pressure in minichannels is found to be equal to vapor saturation pressure, and higher velocity magnitudes especially at the outlet are visible in the microchannels. It is shown that for higher upstream pressures, the cavitating flow extends over the length of the micro/minichannel, thereby increasing the possibility of collapse at the outlet. The effect of energy associated with turbulence was investigated at high Reynolds numbers for both micro/minichannels and its impact was analyzed using wall shear stress, turbulence kinetic energy and mean velocity at various locations of the micro/minichannels.