The Study of Seunggal-tang Gamibang on the Anti-allergy Effects

Ju, Bong-Hyun,Lyu, Sun-Ae,Ko, Woo-Shin,Kim, Own-Il,Lee, Seung-Yeon The Association of Korean Oriental Pediatrics 2007 대한한방소아과학회지 Vol.21 No.2

Objectives The purpose of this study is to investigate effects of Seunggal-tang gamibang (SGT) on the PMA plus A23187-induced RBL-2H3 cells. Methods The effect of SGT on ${\beta}$ -hexosamonidase release, passive cutaneous anaphylaxis, $TNF-{\alpha}$ production and IL-4 secretion was studied. Results SGT inhibited the release of ${\beta}$ -hexosamonidase in RBL-2H3, without affecting cell viability. The passive cutaneous anaphylaxis (PCA) was inhibited by the SGT. Moreover SGT also reduced production of $TNF-{\alpha}$ and secretion of IL-4 in a dose-dependent manner. Conclusions SGT has anti-allergy effects on the RBL-2H3 cells.

The Role of Cyclosporine and Mycophenolate in an Orthotopic Porcine-to-Rat Corneal Xenotransplantation

Lee, Hyeon Il,Kim, Mee Kum,Oh, Joo Youn,Ko, Jung Hwa,Lee, Hyun Ju,Wee, Won Ryang,Lee, Jin Hak The Korean Academy of Medical Sciences 2008 JOURNAL OF KOREAN MEDICAL SCIENCE Vol.23 No.3

<P>We performed this study to investigate the feature of rejection in porcine-to-rat corneal orthotopic transplantation and to evaluate the effect of cyclosporine and mycophenolate on the xeno-rejection. Orthotopic corneal transplantation was done at 91 Sprague-Dawley rats, and they were divided into 10 groups based on the combination of immunosuppressants including dexamethasone, cyclosporine, and mycophenolate mofetil. Graft survival was analyzed and grafted eyes were examined with Hematoxylin & Eosin and CD4 or CD8 staining. Enzyme-linked immunosorbent assays were done for interleukin-2 (IL-2), IL-4, IL-5, IL-10, and interferon (IFN)-γ in cornea, lacrimal gland, and cervical lymph nodes. The longest median survival of the immune suppressant group was 11.00±1.96 days, which showed no statistical differences compared with that of control (8.00±1.52 days). The neutrophils were prominent in the early phase but soon gave way to the monocytes. The number of CD8+ cells was higher than that of CD4+ cells. IL-2 and IFN-γ markedly increased at 10 to13 days in cornea, lacrimal glands, and cervical lymph nodes, which showed a decrease with immunosuppressants except in the cornea. In conclusion, cyclosporine and mycophenolate could not prevent the rejection in porcine to rat orthotopic corneal xenograft associated with infiltraton of CD8+ and innate immune cells.</P>

Erythropoietin priming improves the vasculogenic potential of G-CSF mobilized human peripheral blood mononuclear cells

Kang, Jeehoon,Yun, Ji-Yeon,Hur, Jin,Kang, Jin-A,Choi, Jae-Il,Ko, Seung Bum,Lee, Jaewon,Kim, Ju-Young,Hwang, In-Chang,Park, Young-Bae,Kim, Hyo-Soo Oxford University Press 2014 Cardiovascular research Vol.104 No.1

<P><B>Aims</B></P><P>From our previous clinical trials, intracoronary infusion of granulocyte-colony stimulating factor (G-CSF)-mobilized peripheral blood mononuclear cells (<SUP>mob</SUP>PBMCs) proved to be effective in improving myocardial contractility and reducing infarct volume in acute myocardial infarction. We tested the effect of priming <SUP>mob</SUP>PBMCs with erythropoietin (EPO) to augment its therapeutic efficacy.</P><P><B>Methods and results</B></P><P><SUP>mob</SUP>PBMCs were obtained from healthy volunteers after a 3-day subcutaneous injection of G-CSF (10 μg/kg). About 40% of <SUP>mob</SUP>PBMCs were EPO receptor (EPOR) (+) and responded to 6 h EPO-priming (10 IU/mL) by increasing the expression of vasculogenic factors (i.e. IL8, IL10, bFGF, PDGF, MMP9) and adhesion molecules (i.e. integrin αV, β1, β2, β8) through the JAK2 and Akt pathway. These responses were also observed in PBMCs from elderly patients with coronary disease. The conditioned media from EPO-primed <SUP>mob</SUP>PBMCs contained various cytokines such as IL8, IL10, TNFα, and PDGF, which enhanced the migration and tube formation capability of endothelial cells. EPO-primed <SUP>mob</SUP>PBMCs also showed increased adhesion on endothelial cells or fibronectin. Augmented vasculogenic potential of EPO-primed <SUP>mob</SUP>PBMCs was confirmed in a Matrigel plug assay, ischaemic hindlimb, and myocardial infarction models of athymic nude mice. There were two action mechanisms: (i) cellular effects confirmed by direct incorporation of human <SUP>mob</SUP>PBSCs into mouse vasculature and (ii) indirect humoral effects confirmed by the therapeutic effect of the supernatant of EPO-primed <SUP>mob</SUP>PBMCs.</P><P><B>Conclusion</B></P><P>Brief <I>ex vivo</I> EPO-priming is a novel method to augment the vasculogenic potential of human <SUP>mob</SUP>PBMCs, which would help to achieve better results after intracoronary infusion in myocardial infarction patients.</P>

동영상 인식을 위한 프레임 지식의 제어

고일주,최형일 崇實大學校 生産技術硏究所 1995 論文集 Vol.25 No.2

In this paper, we suggest the frame knowledge representation and the control flow for a moving image analysis. We reconstruct the frame knowledge into the object frame, the behavior frame and the schema. The object frame represents characteristics of target object, the behavior frame represents a behavior of the object frame. And the schema represents a meaning about sequenced moving objects. The schema has the static schema and the dynamic schema. The static schema is a built-in knowledge and the dynamic schema is constructed in recognition process. The control flow recognized the object frame and the behavior frame in each time, constructs the dynamic schema. Then, it matches the dynamic schema with the static schema, and selects a most similar static schema. Finally, the control flow performs recognition of moving images as analyzing the semantic of the selected static schema.

일 도농복합지역 저소득층 노인의 건강문제 분석 : 보건소 방문간호사업 대상자 중심으로

고일선,,이태화,이경자,이정렬,임미혜,천의영,주윤미,이계철 노인간호학회 2005 노인간호학회지 Vol.7 No.2

Purpose: The purpose of this study was to analyze the health problems of low-income aged with chronic illness living in urban-rural composite area. Method: The sample consisted of 440 aged who were receiving home care services from a public health center. MDS-HC was applied to analyze the health problems of the aged. Data were collected through a face- to-face interview by six trained interviewers from June 28 to July 15, 2004. Result: Subjects had average number of 8 health problems in both urban and rural area. Lack of preventive health care measure, pain, and visual function were the most frequent health problem. The rural aged had more pain, bowel management problems, compared to the urban aged having more urinary incontinence and indwelling catheter. There were many health problems related to falls and pressure ulcers with middle-old aged in urban, and old-old aged in rural area. Conclusion: The results of this study showed strategies for care intervention of low-income elderly to put in practice. Therefore, tailored-service for each subject should be provided.

벼꽃으로부터 두 개의 calmodulin cDNA 유전자의 분리 및 특성 연구

고보경,고명수,김유정,정일선,최영주 신라대학교 자연과학연구소 2005 自然科學論文集 Vol.14 No.-

Ca^(2+)은 신호전달과정에서 중요한 작용을 하는데 CaM은 이러한 Ca^(2+)이은의 modulator로서 중요한 작용을 하는 것으로 알러져 있다. 벼꽃의 cDNA library로부터 rice genomic DNA(RCaM-2)를 probe로 사용하여 30여개의 Positive clones을 얻어 이 중 두 개의 벼꽃 calmodulin CDNA clone을 분리하였다. RFCaMs CDNA clone의 총염기수는 RFCaM-3은 831p, RFCaM-4는 751bp이고 open reading frame은 각각 149개의 아미노산으로 구성되어 있다. RFCaM-3 과 RFCaM-4의아미노산은 동일하지만 nucleotide 순서는 상당한 차이를 나타내고 있다. RFCaMs CDNA clone의 open reading flame은 protein serine/threonine kinase에 보존되어 있는 catalytic domain을 가지고 있다 RfCaM-3은 5'-untranslated region은 105bp, 3'-untranslated region은 270bp이고 RfCaM-4는 5'-untranslated region은 78bp, 3'-untranslatedregion은 283bp이다. RFCaMs는 Arabidopsis와 70%, potato와는 92%, carrot과는 98%의 유사성을 보였다. Genomic southern blot분석에 의하면 벼의 clamodulin 유전자들이 7-8개의 multigene family로 존재하는 것으로 나타났다. Two different calmodulin (CaM) cDNAs (RFCaM-3 and RFCaM-4) were isolated from a rice flower cDNA library by screening with a rice genomic probe (RCaM-2). Both cDNAs are 90% sequence homologous inside the coding region but are highly divergent outside this region. The polypeptides encoded by RFCaM-3 and RFCaM-4 show identical sequence homology. Comparison of the deduced amino acid sequence of the RFCaMs cDNA clone have identity to Arabidopsis (70%), potato (92%), carrot (98%), and barley and soybean (98%). Southern analysis revealed that both cDNAs are encoded by different genes. Four intense and a few weaker bands were observed in the restriction fragments. Most restriction fragments showed high intensity hybridization signals under stringent conditions, suggesting the presence of a small multigene family for CaM in the rice genome as in other plants.

문자의 외곽 정보를 이용한 효율적인 특징 추출

고일주,최형일 崇實大學校 生産技術硏究所 1994 論文集 Vol.24 No.-

The role of character recognition is becoming essential, as the amount of document information to be processed is increasing. In the past, the major target of research in character recognition was alphanumeric characters, but recent research is challenging a system that can recognize the Korean and the Chineese Characters as well as the alpanumerics. However, the problem of recognizing oriental characters is much more difficult than that of western characters, since oriental languages have much larger character sets compared to western languages. In order to solve such a problem, a very special care has to be paid in extracting features, since features are main key to recognize characters. That is, it is essential to extract features whose discriminating power is very high. This paper proposed a feature extraction method that uses the contour information of a character. The contour information has known to be very useful in recognizing alphanumeric characters. However, extracting the contour information of oriental characters is not a simple problem, since a oriental character may be splited into several connected components. To solve such a problem, this paper first extracts a minimum enclosing polygon of a character by combining the results of four directional projections. It next applies a background thinning approach to the extracted polygon, and finally it obtains the contour information based on the thinning results.

전자카달로그 특성요인과 고객의 관계품질에 관한 연구

고일상,강주선 한국정보시스템학회 2006 情報시스템硏究 Vol.15 No.1

직무몰입, 조직시민행동에 대한 성과지향적 보상체계의 효과 : 영업직을 중심으로 the Study of Stock Brokers

고수일,한주희 한국생산성학회 2001 生産性論集 Vol.15 No.3

본 연구는 성과-보상의 연계성으로 유발된 직무동기가 직무몰입 및 조직시민행동에 미치는 영향에 대하여 증권회사의 영업직을 대상으로 실증 분석하였다. 성과-보상의 연계성은 성과-승진의 연계성, 성과-급여의 연계성 그리고 성과-평가의 연계성의 하위개념으로 구분하여 고찰하였다. 연구 결과를 요약하면 다음과 같다. 첫째, 연구변수들에 대한 상관관계 분석결과, 성과-급여의 연계성과 성과-평가의 연계성은 정의 상관성을 나타내고 있다. 또한 성과-급여의 연계성은 직무동기, 직무몰입, 의사소통 및 이타성 변수 등과 모두 정의 상관성을 보이고 있었다. 한편 성과-평가의 연계성은 직무동기 및 의사소통과 정의 상관성을 나타내고 있음을 확인할 수 있다. 직무동기는 직무몰입, 의사소통 및 이타성과 모두 정의 상관성을 나타내고 있다. 둘째, 자신의 업무영역이 분명하고 업무 성과에 대한 측정이 가능한 영업직 구성원의 경우에는 성과-급여 및 성과-평가의 연계성을 높이는 제도를 도입하는 경우 직무동기가 높아진다는 것이다. 영업직 구성원은 성과-승진의 연계성을 통하여 동기부여되지 않으며, 또한 승진과 급여를 별개로 인식하고 있음을 알 수 있다. 셋째, 성과지향적 보상체계를 설계하는 인사관리자들은 성과-평가의 연계성을 제고할 수 있는 방안을 찾아야 할 것이다. 본 연구 결과는 성과와 급여의 연계에 집중되어 왔던 기존의 관점에서 성과와 평가의 연계성에 대한 관점으로 확대 또는 전환되어야 할 필요성을 시사하고 있다. This research investigated the relationship of reward for performance, work motivation, job involvement, and organizational citizenship behaviors. With 251 employees in stock brokerage, it was found that (1) pay for performance and evaluation for performance were related positively to employees' work motivation while promotion for performance was not, (2) work motivation showed positive relationship with job involvement and organizational citizenship behaviors such as communication and altruism. thus, (3) reward for performance encouraged in-role and extra-role behaviors at the same time. Some implications of these results were discussed.

쌍직교 웨이블릿 변환을 이용한 계층적 워터마킹

이나영,고일주,김원,김계영 崇實大學校 生産技術硏究所 2002 論文集 Vol.32 No.-

In this paper, we propose a watermarking technique using biorthogonal wavelet transform and hierarchical correlation. The proposed technique inserts a watermark at high frequency domain and improves the computational time for extracting the watermark using hierarchical correlation. In the experiment, we show the performance of the proposed technique and use bar code as watermark to automatically manage the watermarked images.