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Park, Ji-Yeon,Yang, Angel,Park, Jong-Hyouk,Abd El-Aty, A.M.,Oh, Jae-Ho,Do, Jung-Ah,Kwon, Kisung,Shim, Ki-Hoon,Choi, Ok-Ja,Shim, Jae-Han The Korean Society for Applied Biological Chemistr 2012 Applied Biological Chemistry (Appl Biol Chem) Vol.55 No.4
Separation of pesticides and other chemical contaminants from fatty food matrices prior to subsequent steps in the analytical process remains a challenging issue, and much effort has been invested to further enhance this method. The aim of the present study was to develop a simple multi-residue method involving a quick, easy, cheap, effective, rugged, and safe (QuEChERS) extraction method for the identification and quantification of 41 pesticide residues in cooked fatty food matrices, including heated soybean oil, roasted sesame, and boiled soybean using gas chromatography-micro/electron capture detector (GC-${\mu}ECD$). The analytes were subsequently confirmed via GC-mass spectrometry (MS). The responses of analytes were linear with excellent correlation coefficients ($r^2$) ranging from 0.993 to 1.000 (calculated from absolute peak areas). For the majority of the tested pesticides, the mean recoveries ranged from 68.5 and 121.4% with relative standard deviations ranging from 0.4 to 18.7%. Instrument limits of detection and quantification ranged from 0.004 to $0.30{\mu}g/kg$ and 0.0125 to $1.00{\mu}g/kg$, respectively. The developed method presented in this study was applied successfully to determine pesticide residue levels in cooked fatty food matrices. None of the samples contained detectable amounts of pesticide residues.
Park Se Ra,Ko Kisung,Lim Sohee,Cha So Yeon,정현주,Park Soon Ju,Myung Soon‐Chul,Kim Mi Kyung 한국곤충학회 2020 Entomological Research Vol.50 No.4
The monoclonal antibody (mAb) CO17-1A specifically binds to the tumor associated cell surface glycoprotein GA733 in colorectal cancer cells. Thus, mAb CO17-1A has the potential to act as an immune therapeutic protein against colorectal cancer. Recently, it was shown that the baculovirus insect cell expression system produces anti-colorectal cancer mAb CO17-1A. In this study, the colorectal cancer antibody mAb CO17-1A fused to the endoplasmic reticulum (ER) retention signal sequence (KDEL), and the (mAb CO17-1AK) was expressed in Spodoptera frugiperda Sf9 insect cells. The yield, cell cytotoxicity, and in vitro anti-tumor activity of mAb CO17-1AK were verified. Western blotting was performed to confirm that both heavy and light chains of mAb CO17-1A were expressed in Sf9 insect cells. The insect-derived mAb (mAbI ) CO17-1A was purified using a protein G affinity column. An in vitro wound healing assay was conducted to determine the inhibition activity of mAb CO17-1A during tumor cell migration, showing that mAbI CO17-1AK was effective as mammalian-derived mAb CO17-1A (mAbM CO17-1A). These results suggest that the insect cell expression system can produce and properly assemble mAbs that inhibit tumor cell migration.
Park, Da-Young,Lee, Jung-Hwan,So, Yang-Kang,Kim, Young-Kwan,Ko, Kinarm,Park, Sang-Won,Lee, Yong Seok,Han, Yeon Soo,Ko, Kisung Mary Ann Liebert 2011 Hybridoma Vol.30 No.5
<P>The baculovirus-insect cell system is considered a feasible expression system for recombinant glycoprotein production due to its several advantages, including high capacity, flexibility, and glycosylation capability. However, accurate titering of the recombinant baculovirus is required to ensure high expression in insect cells using a commercial and expensive immunoassay titer kit in which the envelope glycoprotein of the Autographa californica multiple nucleopolyhedrovirus (AcMNPV)-type baculovirus is detected by anti-envelope glycoprotein antibody and a secondary antibody conjugated to horseradish peroxidase (HRP). In this study, conditions for the expression of the CO17-1A immunotherapeutic monoclonal antibody (MAb) against colorectal cancer cells in a baculovirus system were optimized without using a commercial titering kit. Several variables were investigated to optimize antibody expression in a baculovirus-insect cell system, including baculovirus passage, volume of the infecting baculovirus inoculum (100, 200, 400, and 800?μL), and the harvest time of insect cells or cell supernatants after virus infection (24, 48, and 72?h). Two different pFastBac vectors carrying the CO17-1A MAb genes with or without the KDEL endoplasmic reticulum (ER) retention motif (Lys-Asp-Glu-Leu) fused to the HC (MAb CO17-1A K and MAb CO17-1A, respectively) were constructed and used to generate baculoviruses. Immunoblot analysis was conducted to confirm expression of MAb CO17-1A K and MAb CO17-1A in baculovirus-infected insect cells. Densitometry analysis of the protein bands was used to quantify the relative expression under different conditions. The highest expression was observed in lysed cells infected with 400?μL of passage 3 baculovirus (P(3) BV) carrying the gene encoding the CO17-1A MAb without KDEL at 72?h after virus infection. These results suggest that the infection conditions, the number of virus passages, baculovirus inoculum volume, and the harvest time can be modified to optimize MAb expression without using a BaculoELISA titer kit in a baculovirus-insect cell system.</P>
그래프 구조 정보를 이용한 효율적인 그래프 메디안 탐색 기법
박기성 ( Kisung Park ),윤영선 ( Youngsun Yun ),김태연 ( Taeyeon Kim ),이영구 ( Young-koo Lee ) 한국정보처리학회 2013 한국정보처리학회 학술대회논문집 Vol.20 No.2
최근 정보 기술의 발달로 XML, 화학 복합물, 소셜 네트워크 등과 같은 구조적 정보를 갖는 빅 데이터들이 대량으로 축적되고 있다. 이러한 구조적 정보를 갖는 그래프 데이터에서 메디안을 찾기 위한 연구가 진행되고 있다. 기존에는 그래프 메디안을 효율적으로 계산하기 위해 하한값을 이용한 그래프 메디안 탐색 기법이 제안되었다. 그러나 탐색을 시작하는 버텍스를 선정하는 데에 따라 가지치기 효과가 다르게 발생하는 문제점이 있다. 본 논문에서는 버텍스의 그래프 구조 정보를 이용한 효율적인 메디안 탐색 기법을 제안한다. 제안하는 탐색 기법은 버텍스의 차수와 에지 가중치를 이용하여 그래프 메디안 예측 값을 정의하고, 그래프 메디안과 유사한 버텍스들부터 우선적으로 탐색한다. 실험을 통하여 제안하는 기법이 기존의 방법보다 최대 10%까지 수행시간을 단축함을 보인다.