식물성 세포벽에 함유된 Ca과 P의 Rooster에 있어서 소화율에 관한 연구

김종쾌,김영규,김대진 東亞大學校 1992 東亞論叢 Vol.29 No.1

본 연구는 綠葉중에서 ladino clover 잎, alfalfa 잎, 칡잎, 아카시아잎, 싸리잎 등을 5월과 6월의 生育시기를 두 시기로 하여 동물 체내에서 Ca과 P 그리고 NDF에 결합된 Ca과 P의 이용성을 조사하였다. 실험에 사용된 동물은 10개월된 재래 숫탉(rooster)으로서 이들 綠葉은 체중 kg당 약 1% 강제 급여(forced feeding) 한 후 36시간 동안의 배설물을 채취하였으며, 이와 동시에 대사성 糞과 내인성 뇨로 분비되는 Ca과 P을 보정하기 위하여 36시간 절식시켜서 배설물을 채취하였다. 생체내 Ca과 P의 이용성 실험은 세번 반복을 하였으며, 대사성 분과 내인성 뇨 실험도 다섯번 반복을 실시하였는데 그 결과를 요약하면 다음과 같다. 1. NDF 含量에 있어서 綠葉(ladino clover잎, alfalfa 잎, 칡잎, 아카시아잎, 싸리잎)은 5월 예취가 30.42%-37.28% 범위였으며, 6월 예취는 45.43-59.36% 점위로 生育이 進行됨에 따라 增加하였다. (p<0.05). 2. 眞正 DM 消化率(TDMD)에 있어서 綠葉은 5월 예취가 24.75-47.24%의 범위였으며, 6월 예취는 23.31-42.20% 범위로 生育이 進行됨에 따라 減少하였다(p<0.01). 3. 眞正 이용 Ca(TACa)에 있어서 綠葉은 5월 예취가 7.94-56.65%의 범위였으며, 6월 예취는 4.02-51.09% 범위로 生育이 進行됨에 따라 alfalfa 잎은 增加하였으나, ladino clover 잎은 減少하였고(p<0.05), 칡잎, 아카시아잎은 뚜렷한 변화가 없었다. 4. 眞正 이용 P(TAP)에 있어서 綠葉은 5월 예취가 8.52-4.89%의 범위였으며, 6월 예취는 24.86-42.6%로 生育이 進行됨에 따라 增加하였으나 ladino clover 잎에서는 減少하였다(p<0.05). 5. NDF에 결합된 Ca의 이용율에 있어서 綠葉은 5월 예취가 4.04-15.80%의 범위였으며, 6월 예취는 3.23-8.11%로 生育이 進行됨에 따라 減少하였으나 (p<0.05), ladino clover 잎, 싸리잎은 뜨렷한 변화가 없었다. 6. NDF에 결합된 P의 이용율에 있어서 綠葉은 5월 예취가 3.97-17.01% 범위였으며, 6월 예취는 4.06-14.93%로 生育이 進行됨에 따라 ladino clover잎, 칡잎, 아카시아잎은 減少하였으나(p<0.05), la-dinl clover 잎과 싸리잎은 뚜렷한 변화가 없었다. 7. 綠葉에 있어서 TDMD(Y) 含量과 NDF 含量(X) 사이에는 Y=53.5-0.42X의 回歸式과 r=-0.50의 相關關係를 보였다(p<0.01). 8. Ladinl clover 잎의 P-D(Y)와 P 含量(X) 사이에는 Y=84.5-85.1X의 回歸式과 r=-0.91의 相關關係를 보였다(p<0.01). 9. Alfalfa 잎의 Ca-D(Y)와 Ca含量(x) 사이에는 Y=18.9+13.0X의 回歸式과 r=0.99의 相關關係를 보였다(p<0.01). 10. 칡잎의 P-D(Y)와 P含量(X) 사이에는 Y=139-368X의 回歸式과 r=-0.95의 相關關係를 보였다(p<0.01). 11. 아카시아잎의 P-D(Y)와 P含量(X) 사이에는 Y=87.8-229X의 回歸式과 r=-0.98의 相關關係를 보였다(p<0.01). 12. 싸리잎의 Ca-D含量(Y)과 Ca含量(X) 사이에는 Y=17.9-12.8X의 回歸式과 r=-0.83의 相關關係를 보였다(p<0.01). This experiment was conducted to evaluate the Ca and P bioavailabilities of green leaf meal by roos-ter. The cell wall binding Ca and P of green leaf meal such as ladino clover, alfalfa, kudzu, acacia and bush clover were collected by two stage of cutting for May and June 1990. Rooster employed in the present experiment were fed about one percentage of body weight by forced feeding of ingredient. Collection of excreta as fed after 36 hous and did not given ingredient during the determinate of urinary endogenous and metabolic fecal Ca, P and excreta for 36 hours. The feeding experiment was conducted triplication for bioassay of Ca and P and was give five repli-cation for composition of metabolic and endogenous material. The results obtained are summarized as follows : 1. The contents of neutral detergent fiber(NDF) of several green leaves meal were ranged from 30.42% to 37.28% for cutting of May and were ranged from 45.43% to 59.36% for cutting of June, there-fore NDF were decreased by abvanced stages of maturity(p<0.05). 2. The true dry matter digestibility(TDMD) of several green leaves meal were ranged from 24.57% to 42.24% for cutting of May and were ranged from 23.31% to 42.20% for cutting of June and TDMD was decreased by advanced stages of maturity(p<0.05). 3. The true available clacium(TACa) of several green leaves meal were ranged from 7.94% to 56.65% for cutting of May and were ranged from 4.02% to 51.09% for cutting of June, exception of alfalfa among other did not show increase by advanced stages of maturity(p<0.05). 4. The true avilable phosphorus(TAP) of several green leaves meal were ranged from 8.25% to 46.89% for cutting of May and were ranged from 24.86% to 42.6% for cutting of June and TAP was increased but decreased only ladino clover by advanced stages of maturity(p<0.05). 5. NDF binding of avilable calcium(NDF+Ca) of several green leaves meal were ranged from 4.04% to 15.80% for cutting of May and were ranged from 3.23% to 50 8.11% for cutting of June and they were decreased by advanced stages of maturity(p<0.05) but ladino clover and bush clover did not changed by advanced stage of maturity. 6. NDF binding of avilable phosphorus(NDF+P) of several green leaves meal were ranged from 3.97% to 17.01% for cutting of May and were ranged from 4.06% to 14.93% for cutting of June and they were decreased by advanced stages of maturity(p<0.05) but alfalfa and bush clover did not changed by advanced stage of maturity. 7. Regression equation and correlation coefficients of several green leaves meal between TDMD(Y) and NDF(X) were Y=53.5=0.42X and r=-0.50(p<0.01). 8. Regression equation and correlation coefficients of ladino clover leaf meal between digestible phos-phorus(Y) and phosphorus(X) were Y=84.5-85.1X and r=-0.91(p<0.01). 10. Regression euqation and correlation coefficients of kudzu leaf meal between digestible phosphours(Y) and phosphours(X) were Y=139-368X and r=-0.95(p<0.01). 11. Regression euqation and correlation coefficients of acacia leaf meal between digestible phosphours(Y) and phosphours(X) were Y=87.8-229X and r=-0.98(p<0.01). 12. Regression euqation and correlation coefficients of bush clover leaf meal between digestible calcium(Y) and calcium(X) were Y=17.9-12.8X and r=-0.83(p<0.05).

200 GeV/핵자 유황이온과 핵건판핵의 충돌에 의해 생성된 헬륨 파쇄핵의 극한파쇄 연구

김동철,송진섭,윤천실,정성헌,박인곤,김종오,김철수,김태연,이승희,조재희,천병구,김재률,김준원,김태익,박명렬,장한일,임인택 慶尙大學校 기초과학연구소 1992 基礎科學硏究所報 Vol.8 No.-

고에너지 중이온 원자핵과 핵건판의 충돌에서, 200GeV/핵자 유황이온에 의해 생성된 파쇄 헬륨핵(Z=2)의 실험실계의 방출각 분포는 표적핵에 무관한 회귀공식. dN=exp[a+k exp(η-y_b)]d[exp(η-y_b)]로 잘 표현된다. 여기에서 의사신속도 η=-ln[tan(θ/2)]이고, y_b는 실험실계의 입사입자(^32S)의 신속도이다. 이 공식에 의한 적합에서 k=-0.057±0.008로 얻어진다. 즉, 핵건판과 고에너지 중이온의 충돌에서 파쇄 헬륨핵의 exp(η-y_b)의 분포는 "극한파쇄" 현상을 잘 설명하고 있다. The angular distribution of emission angle θ of helium (Z=2) produced in the collisions of incident particles of 200 GeV/nucleon ^32S in nuclear emulsion is well expressed by dN=exp[a+k exp(η-y_b)]d[exp(η-y_b)] where the pseudorapidity is η=-ln[tan(θ/2)], the laboratory system primary rapidity is y_b, and k=-0.057+0.008. The shape of this frequency of occurrence distributions in terms of exp(η-y_b) attests to the validity of the concept of "limiting fragmentation" for helium projectile fragments produced in the projectile fragmentation regions of heavy ion collisions in nuclear emulsion.

Cyclobuxine D의 토끼 적출 장관에 대한 작용

이종화,박영현,조병헌,김종배,김정목,김유재,김천숙,차영덕,김영석 최신의학사 1988 最新醫學 Vol.31 No.1

Implication of intracellular ROS formation, caspase-3 activation and Egr-1 induction in platycodon D-induced apoptosis of U937 human leukemia cells

Shin, D.Y.,Kim, G.Y.,Li, W.,Choi, B.T.,Kim, N.D.,Kang, H.S.,Choi, Y.H. Masson Pub. USA, Inc 2009 BIOMEDICINE AND PHARMACOTHERAPY Vol.63 No.2

Platycodon D is a major constituent of triterpene saponins found in the root of Platycodon grandiflorum, Platycodi Radix, which is widely used in traditional Oriental medicine for the treatment of many chronic inflammatory diseases. The results of previous studies have shown that this compound has in vitro growth-inhibitory activity in human cancer cells, however, the mechanism by which this action occurs is poorly understood. In this study, we examined the effects of platycodon D on the production of reactive oxygen species (ROS) and evaluated the association of these effects with apoptotic tumor cell death using a human leukemic U937 cell line. The results of this study demonstrate that platycodon D mediates ROS production, and that this mediation is followed by a decrease in mitochondrial membrane potential (MMP, ΔΨ<SUB>m</SUB>), activation of caspase-3, and cleavage of poly (ADP-ribose) polymerase (PARP). Both the cytotoxic effects and apoptotic characteristics induced by platycodon D treatment were significantly inhibited by z-DEVD-fmk, a caspase-3 inhibitor, which demonstrates the important role that caspase-3 plays in the observed cytotoxic effect. Additionally, the transcription factor early growth response-1 (Egr-1) gene was transcriptionally activated and the levels of non-steroidal anti-inflammatory drug (NSAID)-activated gene-1 (NAG-1) protein were elevated in platycodon D-treated U937 cells. However, the quenching of ROS generation in response to treatment with a ROS scavenger, N-acetyl-l-cysteine, reversed the platycodon D-induced apoptosis effects via inhibition of Egr-1 activation, ROS production, MMP collapse, and the subsequent activation of caspase-3. Although further studies are needed to demonstrate that increased expression of Egr-1 by platycodon D leads directly to NAG-1 induction and subsequent apoptosis, our observations clearly indicate that ROS induced through Egr-1 activation are involved in the early molecular events involved in the platycodon D-induced apoptotic pathway.

비타민 D3 의 경구투여 또는 자외선 조사가 브로일러 병아리의 증체 및 비타민 D3 대사에 미치는 영향

장윤환(Y . H . Chiang),황선일(S . I . Hwang),김중달(J . D . Kim),M . F . Holick(M . F . Holick) 한국영양사료학회 1995 韓國營養飼料學會誌 Vol.19 No.6

Pepsin - Cellulase 에 의한 국내산 주요 조사료의 DMD 에 관한 연구 (3) 주요목초의 세포벽 구성물질과 건물소화율 (乾物消化率)

김대진(D . J . Kim),김영길(Y . K . Kim),황태기(T . K . Hwang),맹원재(W . J . Maeng) 한국영양사료학회 1989 韓國營養飼料學會誌 Vol.13 No.4

Effects of calcium doping on the superconducting properties of top-seeded melt growth processed Y_1.5Ba_2-xCa_xCu₃O_y superconductors

Park, S.D.,Kim, H.J.,Park, B.J.,Han, Y.H.,Jun, B.H.,Lee, J.S.,Kim, C.J. North-Holland 2011 Physica. C, Superconductivity Vol.471 No.21

The effect of calcium doping on the superconducting properties of top seeded melt growth (TSMG) processed Y<SUB>1.5</SUB>Ba<SUB>2-x</SUB>Ca<SUB>x</SUB>Cu<SUB>3</SUB>O<SUB>y</SUB> superconductors was studied in terms of calcium content (X<SUB>ca</SUB>). YBa<SUB>2-x</SUB>Ca<SUB>x</SUB>Cu<SUB>3</SUB>O<SUB>7-δ</SUB> (X<SUB>ca</SUB>=0, 0.005, 0.01, 0.02, 0.04, 0.1, 0.3) powders were synthesized by the powder calcination method. YBa<SUB>2-x</SUB>Ca<SUB>x</SUB>Cu<SUB>3</SUB>O<SUB>7-δ</SUB> powders were mixed with 0.25mole Y<SUB>2</SUB>O<SUB>3</SUB> powder and 1wt.% CeO<SUB>2</SUB> as Y<SUB>2</SUB>BaCuO<SUB>5</SUB> (Y211) refiner, and finally made into Y<SUB>1</SUB>.<SUB>5</SUB>Ba<SUB>2-x</SUB>Ca<SUB>x</SUB>Cu<SUB>3</SUB>O<SUB>y</SUB> (Y1.5)+1wt.% CeO<SUB>2</SUB> composition. The single Y123 growth on the top surface was observed up to X<SUB>ca</SUB>=0.1, while the multiple Y123 growth was observed at X<SUB>ca</SUB>≥0.1. The superconducting transition temperature (T<SUB>c</SUB>) and critical current density (J<SUB>c</SUB>) of TSMG processed Y1.5 samples were inversely proportional to X<SUB>ca</SUB>. The Y211 size increased with increasing X<SUB>ca</SUB> due to the enhancement of Y211 coarsening by calcium doping. No Y211 refining effect by CeO<SUB>2</SUB> was observed in the calcium doped samples. The T<SUB>c</SUB> and J<SUB>c</SUB> decrease by calcium doping are likely to be due to the calcium incorporation with the Y123 lattice and formation of coarse Y211 particles.

칡의 대량생산 이용과 생리활성물질에 관한 연구 (2) 당밀 , 칡즙 및 칡뿌리의 첨가가 칡사일리지의 발효품질과 사료적 가치에 미치는 영향

김대진(D . J . Kim),김영규(Y . K . Kim),맹원재(W . J . Maeng) 한국영양사료학회 1994 韓國營養飼料學會誌 Vol.18 No.2

Patterning of ferroelectric poly(vinylidene fluoride-trifluoroethylene) film for nonvolatile memory devices

Kim, W.Y.,Ka, D.Y.,Kwon, I.W.,Kim, D.S.,Lee, Y.S.,Kim, S.Y.,Lee, H.C. Elsevier 2011 CURRENT APPLIED PHYSICS Vol.11 No.3

Ferroelectric polymer, poly(vinylidene fluoride-trifluoroethylene), was spin-coated to be a thin film. Afterward, solubility relations for several solvents were tested for photo-lithographical patterning of ferroelectric polymer film. Based on solubility relations, the conditions for microscale patterning of ferroelectric polymer film were established with commercialized photoresist and developer and stripper. On the finally pattered film, top electrode of Au was deposited through thermal evaporation and electric displacement-electric field (D-E) relation was measured. Compared to the not-patterned film, any chemical degradation was not found from hysteresis loop. Therefore, it is concluded that the ferroelectric polymer film can be patterned by ordinary photo-lithographical method without chemical degradation.

Exendin-4 induction of cyclin D1 expression in INS-1 beta-cells: involvement of cAMP-responsive element.

Kim, M-J,Kang, J-H,Park, Y G,Ryu, G R,Ko, S H,Jeong, I-K,Koh, K-H,Rhie, D-J,Yoon, S H,Hahn, S J,Kim, M-S,Jo, Y-H Journal of Endocrinology, Ltd. [etc.] 2006 The Journal of endocrinology Vol.188 No.3

<P>Glucagon-like peptide-1 (GLP-1) and its analog exendin-4 (EX) have been considered as a growth factor implicated in pancreatic islet mass increase and beta-cell proliferation. This study aimed to investigate the effect of EX on cyclin D1 expression, a key regulator of the cell cycle, in the pancreatic beta-cell line INS-1. We demonstrated that EX significantly increased cyclin D1 mRNA and subsequently its protein levels. Although EX induced phosphorylation of Raf-1 and extracellular-signal-regulated kinase (ERK), both PD98059 and exogenous ERK1 had no effect on the cyclin D1 induction by EX. Instead, the cAMP-elevating agent forskolin induced cyclin D1 expression remarkably and this response was inhibited by pretreatment with H-89, a protein kinase A (PKA) inhibitor. Promoter analyses revealed that the cAMP-responsive element (CRE) site (at position -48; 5'-TAACGTCA-3') of cyclin D1 gene was required for both basal and EX-induced activation of the cyclin D1 promoter, which was confirmed by site-directed mutagenesis study. For EX to activate the cyclin D1 promoter effectively, CRE-binding protein (CREB) should be phosphorylated and bound to the putative CRE site, according to the results of electrophoretic mobility shift and chromatin immunoprecipitation assays. Lastly, a transfection assay employing constitutively active or dominant-negative CREB expression plasmids clearly demonstrated that CREB was largely involved in both basal and EX-induced cyclin D1 promoter activities. Taken together, EX-induced cyclin D1 expression is largely dependent on the cAMP/PKA signaling pathway, and EX increases the level of phosphorylated CREB and more potently trans-activates cyclin D1 gene through binding of the CREB to the putative CRE site, implicating a potential mechanism underlying beta-cell proliferation by EX.</P>