경북 성주지역 장수노인의 영양상태(Ⅰ)

백지원(Baek, Ji-Won),구보경(Koo, Bo-Kyung),김규종(Kim, Kyu-Jong),이연경(Lee, Yeon-Kyung),이성국(Lee, Sung-Kook),이혜성(Lee, Hye-sung) 한국영양학회 2000 Journal of Nutrition and Health Vol.33 No.4

경북 성주지역 장수노인의 계절별 식품섭취 상태

백지원(Ji-Won Baek),구보경(Bo-Kyung Koo),김규종(Kyu-Jong Kim),이연경(Yeon-Kyung Lee),이성국(Sung-Kook Lee),이혜성(Hye-Sung Lee) 한국식품영양과학회 2000 한국식품영양과학회지 Vol.29 No.4

본 연구는 우리나라 장수노인들의 식품섭취상태를 분석 평가하고 건강한 장수를 위한 식생활 지침의 설정을 위한 기초자료를 얻고자 수행되었다. 연구 대상은 경상북도 성주군에 거주하는 85세 이상 고령노인 중에서 일상 생활에 문제가 없는 224명이었으며 이들을 대상으로 반복 24시간 회상법을 이용하여 1년간 4회의 계절별 식품 섭취 조사를 실시하였으며 결과를 요약하면 다음과 같다. 계절별 식품군별 섭취량에 있어서 남자노인은 감자류의 섭취량이 여름철에 유의적으로 높고, 버섯류의 섭취량이 가을철에 유의하게 높은 것을 제외하고는 모든 식품군의 섭취량에 있어 계절별 차이가 나타나지 않았다. 여자노인의 경우 곡류, 당류, 두류, 채소류, 과일류, 조미료류, 음료 및 주류, 육류, 우유류, 어패류의 섭취량이 겨울철에 유의하게 높았으며, 종실류, 해조류, 난류의 섭취량은 계절에 따른 차이가 없었다. 조사 대상자들의 연평균 1일 총 식품 섭취량은 594.4±186.1 g이었으며 이 중에서 513.5±161.2 g(86.4%)는 식물성 식품군으로부터 섭취하였고 79.3±56.7 g(13.3%)는 동물성 식품군으로부터 섭취하였다. 섭취량이 가장 높았던 식품군은 곡류였으며그 다음이 채소류, 과일류, 어패류, 음료 및 주류, 조미료류, 두류, 육류, 우유류, 감자류 등의 순이었다. 장수 노인대상자들의 음식별 섭취빈도는 밥류에 있어서는 쌀밥의 섭취비율이 80.1~89.2%로 가장 높았으며 국과 찌개류에서는 쇠고기국, 된장국, 된장찌개의 섭취 비율이 높았다. 어육류 식품의 경우 조기구이의 섭취비율(9.4~12.7%)이 높았으며, 해조류 식품의 경우 김구이의 섭취비율(7.4~20.5%)이 높았고, 각 계절마다 계절식품의 섭취비율이 높았다. 김치류의 경우 배추김치의 섭취비율이 4계절 모두 70% 이상으로 높게 나타났다. 일품요리류는 손쉽게 조리할 수 있는 라면과 국수의 섭취비율이 높았다.<br/> 본 연구의 결과에서 경북 성주지역 장수 노인들의 계절별 식품 섭취량에 있어 겨울철의 식품 섭취량이 다른계절에 비해 유의하게 높았으며, 남자노인보다는 여자노인이 식품섭취에 계절에 의한 영향을 더욱더 많이 받는 것으로 나타났다. 또한 장수 노인들은 가공식품보다는 계절마다 제철에 생산되는 자연 식품의 섭취비율이 높았다. 전반적으로 장수노인들은 소식의 경향을 보였으며 이와 같은 소식습관과 신선한 식물성 식품들의 일상 섭취가 건강한 장수에 영향을 미쳤을 가능성도 있을 것으로 사료된다. The purpose of this study was to estimate food intakes of the long-lived elderly and to obtain the data for establishing dietary guidelines that may be recommended for the general population for the sake of longevity. The subjects of the study were 224 elderly people of age over 85 years living in Kyungpook Sung-Ju area who have no problem in daily living. The food consumption survey was carried out seasonally by the repeated 24-hr recall method for one year. The subject group for this study was composed of 58 males and 166 females, the average age being 87 years old. Food intakes of the winter were more than any other seasons. The mean daily total food intake per capita was 594.4 g, 513.5 g (86.4%) from plant foods and 79.3 g (13.3%) from animal foods. The sequence of high intakes of food groups were cereals, vegetables, fruits, fishes, legumes and meats. Boiled white rice, beef soup, soybean paste soup, soybean paste stew, broiled yellow croaker, kimchi, ra myon and broiled noodles were consumed most frequently. In conclusion, the subjects consumed much more plants foods than animal foods and consumed more natural and seasonal foods than processed foods and also showed food habits of eating small meals. Dietary habits of eating small meals containing abundant amount of fresh plant foods might partially contribute to the longevity of the subjects.

Antigenieity Study of CFA-001,Cefazolin,a Cephalosperin Derivative Produced by an Enzymatic Semisynthesis

Park, Jong Il,Han, sang seop,Jeong, Tae Cheon,Roh, Jung Koo,Kim, Hyoung Chin,Kim, Jeong Hwan,Jeon, Yeong Joong,Kim, Dal Hyun,Kim,Je Hak,Park, Kwan Ha 한국응용약물학회 1997 Biomolecules & Therapeutics(구 응용약물학회지) Vol.5 No.1

The antigenic potential of CFA-001, cefazolin, a cephalosporin derivative produced by an enzymatic semisynthesis, was determined in Hartley guinea pigs. A battery of tests employed consisted of active systemic anaphylaxis (ASA), passive cutaneous anaphylaxis (PCA), and indirect hemagglutination test (IHA). The results were as follows: 1) In ASA, no signs attributable to anaphylaxis was observed in guinea pigs sensitized with CFA-001, whereas OVA-sensitized animals induced severe anaphylactic symptoms; 2) guinea pigs did not produce antibodies against CFA-001 when sensitized with or without Freund's complete adjuvant (FCA) in homologous PCA tests. Meanwhile, antibodies against ovalbumin (OVA) were clearly detected; 3) No CFA-001-specific hemagglutination was observed in the IHA using sera obtained from CFA-001-sensitized guinea pigs. These results suggest that CFA-001 has no antigenicity potential in guinea pigs.

Nonstop Monomer-to-Aramid Nanofiber Synthesis with Remarkable Reinforcement Ability

Koo, Jun Mo,Kim, Hojun,Lee, Minkyung,Park, Seul-A,Jeon, Hyeonyeol,Shin, Sung-Ho,Kim, Seon-Mi,Cha, Hyun Gil,Jegal, Jonggeon,Kim, Byeong-Su,Choi, Bong Gill,Hwang, Sung Yeon,Oh, Dongyeop X.,Park, Jeyoung American Chemical Society 2019 Macromolecules Vol.52 No.3

<P>Aramid nanofibers (ANFs), typically produced by exfoliating aramid microfibers (Kevlar) in alkaline media, exhibit excellent mechanical properties and have therefore attracted increased attention as nanoscale building blocks. However, the preparation of aramid microfibers involves laborious and hazardous processes, which limits the industrial-scale use of ANFs. This work describes a facile and direct monomer-to-ANF synthesis via an as-synthesized intermediate low-molecular-weight poly(<I>p</I>-phenylene terephthalamide) (PPTA) without requiring the environmentally destructive acids and high-order shearing processes. Under the employed conditions, PPTA immediately dissociates and self-assembles into ANFs within a time period of 15 h, which is much shorter than the time of 180 h (not including the Kevlar preparation time) required for the Kevlar-to-ANF conversion. Interestingly, the fabricated ANFs exhibit nanoscale dimensions and thermoplastic polyurethane (TPU) reinforcing effects similar to those of Kevlar-derived ANFs; i.e., a 1.5-fold TPU toughness improvement and a maximum ultimate tensile strength of 84 MPa are achieved at an ANF content of only 0.04 wt %. Remarkable reinforcement ability investigated by comprehensive analytical data comes from ANFs, which disturb ordered hydrogen bonding in hard segments and induce strain hardening along the elongation pathway. Thus, the developed approach paves the way to industrial-scale production of ANFs and related nanocomposites.</P> [FIG OMISSION]</BR>

Production of polyclonal antibody against recombinant ORF 112 L of rock bream (Oplegnathus fasciatus) iridovirus (RBIV) and in vitro neutralization.

Kim, Yun Im,Ha, Yu Mi,Nam, Yoon Kwon,Kim, Ki Hong,Kim, Sung Koo The Academy 2008 Journal of environmental biology Vol.29 No.4

<P>Rock bream iridovirus (RBIV) is a causative agent of epizootics among cultured rock bream (Oplegnathus fasciatus) in Korea. In this study, the immunogenic property of ankyrin repeats gene (ORF 112L) from RBIV was evaluated to develop vaccines against RBIV. ORF 112L of RBIV was cloned into expression vector of pGEX-4T-1. The recombinant protein was successfully expressed using E. coli BL21 (DE3). The soluble recombinant RBIV protein was applied to affinity column for the purification of the protein. Mice were immunized by the injection of purified recombinant protein to produce polyclonal antibodies. EUSA was carried out to identify the immune reaction abilities of polyclonal antibody to recombinant protein. The antigenic property of this protein was evaluated by using in vitro neutralization with BF-2 cells. In neutralization test, BF-2 cells infected with the mixture of RBIV and antisera containing anti-GST-ORF 112L polyclonal antibody were healthy showing few cytopathic effect (CPE) similar with the negative control (without RBIV). These studies suggest that the protein from the ankyrin repeats gene, ORF 112L of RBIV may play an important role in the mechanism of infection. Also, it can be possible to develop protein or gene vaccines using ORF 112L against RBIV.</P>

Identification and characterization of alternative promoters of the rice MAP kinase gene OsBWMK1

Koo, Sung Cheol,Choi, Man Soo,Chun, Hyun Jin,Park, Hyeong Cheol,Kang, Chang Ho,Shim, Sang In,Chung, Jong Il,Cheong, Yong Hwa,Lee, Sang Yeol,Yun, Dae-Jin,Chung, Woo Sik,Cho, Moo Je,Kim, Min Chul Springer-Verlag 2009 Molecules and cells Vol.27 No.4

<P>Our previous study suggested that OsBWMK1, a gene which encodes a member of the rice MAP kinase family, generates transcript variants which show distinct expression patterns in response to environmental stresses. The transcript variants are generated by alternative splicing and by use of alternative promoters. To test whether the two alternative promoters, pOsBWMK1L (promoter for the OsBWMK1L splice variant) and pOsBWMK1S (promoter for the OsBWMK1S splice variant), are biologically functional, we analyzed transgenic plants expressing GUS fusion constructs for each promoter. Both pOsBWMK1L and pOsBWMK1S are biologically active, although the activity of pOsBWMK1S is lower than that of pOsBWMK1L. Histochemical analysis revealed that pOsBWMK1L is constitutively active in most tissues at various developmental stages in rice and Arabidopsis, whereas pOsBWMK1S activity is spatially and temporally restricted. Furthermore, the expression of pOsBWMK1S::GUS was upregulated in response to hydrogen peroxide, a plant defense signaling molecule, in both plant species. These results suggest that the differential expression of OsBWMK1 splice variants is the result of alternative promoter usage and, moreover, that the mechanisms controlling OsBWMK1 gene expression are conserved in both monocot and dicot plants.</P>

A Fatal Case of Oxycodone Intoxication

Sang-Whan In,Hye-Eun Lee,Ji-Hyun Kim,Won-Im Do,Yu-Jin Park,Young-Ho Moon,Ki-Ser Koo 한국법과학회 2005 한국법과학회 학술대회 Vol.2005 No.1

WHY GAME PLAYERS PURCHASE LUXURY ITEMS IN ONLINE GAME? - FOCUSING UPON LUXURY SKIN IN LEAGUE OF LEGENDS

Sang Jin Kim,Kyong Ryul Koo,Honglei Liu,Kyung Hoon Kim 글로벌지식마케팅경영학회 2015 Global Fashion Management Conference Vol.2015 No.06

The online game market has grown rapidly in worldwide. The world game market earned 111.7 billion US dollars and online game occupies 18.9% (21.1 billion US dollars) in the world game market in 2012. Online game companies have launched a variety of free online games to online game players such as League of Legends (LOL), World of Tank, and Hearthstone. These online games provide online game with free install with online game players. Online game companies, however, sell some of online game items to the game users. For example, LOL sells Skin that is the cloth which only provides fancy effect to online game hero through the online game shop. In case of ‘Hearthstone: Heroes of Warcraft’, game user can purchase game card deck by cash. This study was initiated to answer the following research question called “How these online game companies get profit?” because their online games are free to play. The research upon the question mentioned above leads to the second research question called “how online game users purchase the cash game items?”. To understand purchasing behavior and attitude of online game players about cash game item, this study conducts focus group interview of LOL game player to understand purchase behavior of game players for online games items. The results of focus group interview help us to understand the relationship between attitude toward online game items and consumption values. The purposes of this study are 1) to understand online game players’ purchasing behavior for LOL luxury Skin, 2) to find out relationships among online game experience, design innovative Skin, consumption values, and repurchase intention, and 3) to draw academic and practical implications based upon the result of analysis in this study.

Free-breathing dynamic contrast-enhanced MRI of the abdomen and chest using a radial gradient echo sequence with K-space weighted image contrast (KWIC).

Kim, Kyung Won,Lee, Jeong Min,Jeon, Yong Sik,Kang, Sung Eun,Baek, Jee Hyun,Han, Joon Koo,Choi, Byung Ihn,Bang, Yung-Jue,Kiefer, Berthold,Block, Kai Tobias,Ji, Hyunjun,Bauer, Simon,Kim, Chin Springer International 2013 European radiology Vol.23 No.5

<P>To evaluate the feasibility of free-breathing, dynamic contrast-enhanced (DCE) MRI of the abdomen and thorax using the radial-gradient-echo sequence with k-space weighted image contrast (KWIC) reconstruction.</P>

Rapid Diagnosis of Tick-Borne Illnesses by Use of One-Step Isothermal Nucleic Acid Amplification and Bio-Optical Sensor Detection

Kim, Ji Yeun,Koo, Bonhan,Jin, Choong Eun,Kim, Min Chul,Chong, Yong Pil,Lee, Sang-Oh,Choi, Sang-Ho,Kim, Yang Soo,Woo, Jun Hee,Shin, Yong,Kim, Sung-Han American Association for Clinical Chemistry, Inc. 2018 Clinical chemistry Vol.64 No.3

<P><B>BACKGROUND:</B></P><P>Scrub typhus and severe fever with thrombocytopenia syndrome (SFTS) are the most common tick-borne illnesses in South Korea. Early differentiation of SFTS from scrub typhus in emergency departments is essential but difficult because of their overlapping epidemiology, shared risk factors, and similar clinical manifestations.</P><P><B>METHODS:</B></P><P>We compared the diagnostic performance of one-step isothermal nucleic acid amplification with bio-optical sensor detection (iNAD) under isothermal conditions, which is rapid (20–30 min), with that of real-time PCR, in patients with a confirmed tick-borne illness. Fifteen patients with confirmed SFTS who provided a total of 15 initial blood samples and 5 follow-up blood samples, and 21 patients with confirmed scrub typhus, were evaluated.</P><P><B>RESULTS:</B></P><P>The clinical sensitivity of iNAD (100%; 95% CI, 83–100) for SFTS was significantly higher than that of real-time PCR (75%; 95% CI, 51–91; <I>P</I> = 0.047), while its clinical specificity (86%; 95% CI, 65–97) was similar to that of real-time PCR (95%; 95% CI, 77–99; <I>P</I> = 0.61). The clinical sensitivity of iNAD for scrub typhus (100%; 95% CI, 81–100) was significantly higher than that of real-time PCR for scrub typhus (67%; 95% CI, 43–85; <I>P</I> = 0.009), while its clinical specificity (90%; 95% CI, 67–98) was similar to that of real-time PCR (95%; 95% CI, 73–100; <I>P</I> > 0.99).</P><P><B>CONCLUSIONS:</B></P><P>iNAD is a valuable, rapid method of detecting SFTS virus and <I>Orientia tsutsugamushi</I> with high clinical sensitivity and specificity.</P>