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Hyunah Kim,Hyun Kyung Kim,Hyun-Na Koo,Gil-Hah Kim 한국응용곤충학회 2014 한국응용곤충학회 학술대회논문집 Vol.2014 No.10
The two spotted mite, Tetranychus urticae Koch, is a global pest, and has developed severe resistance to several types of acaricides. Acequinocyl-resistant strain and bifenazate-resistant strain of T. urticae showed higher relative activity level of glutathion-S-transferases (GSTs) than susceptible strain. Studies of resistance in T. urticae have focused on classical detoxifying enzyme system, such as GSTs. We examined the expression of six GSTs genes in T. urticae belong to five different cytosolic classes, including two in delta, one in omega, one in mu, one in zeta, and one mitochondrial GSTs class, kappa. RT-qPCR results showed that, among these six GSTs genes, the relative expression level of delta 1 was significantly higher in bifenazate-resistant strain than in other strains (susceptible, abamectin, acequinocyl, etoxazole and pyridaben strain). Expression level of the delta 2 was also higher in acequinocyl-resistant strain than in other strains. The elevated GSTs gene transcripts following exposure to acequinocyl, bifenazate might be one of the mechanisms involved in detoxification of these acaricide. These results may be caused by different mechanisms in the resistance development of T. urticae to these two different types of acaricides.
Future Prospects of Health Management Systems Using Cellular Phones
Kim, Hun-Sung,Hwang, Yunji,Lee, Jae-Ho,Oh, Hye Young,Kim, Yi-Jun,Kwon, Hyeon Yoon,Kang, Hyoseung,Kim, Hyunah,Park, Rae Woong,Kim, Ju Han Mary Ann Liebert 2014 TELEMEDICINE JOURNAL AND E HEALTH Vol.20 No.6
<P>Cellular phones enable communication between healthcare providers and patients for prevention, diagnosis, and treatment of diseases. However, few studies have examined the user-friendliness or effectiveness of cellular phone-based medical informatics (CPBMI) for healthcare.</P>
Sung Il Kim,Won Jin Kang,Yeseul Choi,Hyunah Kim,Bueyong Park,Hyun Kyung Kim,Hyun-Ha Koo,Gil-Hah Kim 한국응용곤충학회 2018 한국응용곤충학회 학술대회논문집 Vol.2018 No.04
The two-spotted spider mite, Tetranychus urticae Koch (Acari: Tetranychidae), is one of the most important pest species devastating many horticultural and ornamental crops and fruit trees. In the present study, a field strain was collected in January 2001 and has been selected for sixteen years with acequinocyl. The resistance ratio calculated from the LC50 of eggs and adults was 191- and 4,237-fold, respectively. The laboratory-selected acequinocyl resistance (LSAR16) strain was screened with 11 acaricides for cross-resistance. The detoxifying enzyme activities and quantitative real-time PCR analysis were performed in the LSAR16 strain. Crossing experiments revealed that LSAR16 strain was inherited maternally, incompletely dominant and monogenic. Most importantly, we identified two new point-mutations at mitochondrial cytb from acequinocyl resistant T. urticae.
Sung-chan Yang,Byung-Eon Noh,Eun Jung Lee,Bong Gu song,Hyunwoo Kim,Tea-Kyu Kim,Seong Yoon Kim,Won Il Park,Hyung-Woo Lim,Hak Seon Lee,Gi-Hun Kim,Chang won Jang,Jin Hwan Jeon,Se Jin Jeong,Hyunah Kim,Kyu 한국응용곤충학회 2017 한국응용곤충학회 학술대회논문집 Vol.2017 No.04
We performed a survey for flavivirus infection and distribution of Aedes albopictus that known as Zika and Dengue virus vector using black–light trap and BG-sentinel trap around urban area in Korea. Mosquitoes were collected in 27 cities during March to November (twice a month) year 2016. Total numbers of mosquitoes collected 102,102 including 19 species 8 genera during collecting period. Total 21,467 Ae. albopictus was collected that 20,961(24.3%) by BG-sentinel trap and 506 (3.2%) by Black-light trap in urban area. Trap index(trap/night) of Ae. albopictus was showed highest in Hamyang (TI:992.3) and lowest in Taebaek (TI:0.3) there was only collected by Black-light trap. A total of 894 pools from all collecting Ae. albopictus were performed a Flavivirus detection. Flavivirus was not detected during study period. This study may provide basic information for surveillance of imported diseases (include Zika virus) and vectors in Korea.
Hyunah Kim,Dong-jik Lee,Doo-Byoung Oh,Ohsuk Kwon,HyunAh Kang 한국당과학회 2011 한국당과학회 학술대회 Vol.2011 No.1
Protein O-mannosylation is evolutionarily conserved essential protein modification from bacteria to humans, which is initiated in the ER by protein O-mannosyltransferases (PMTs) that catalyze the transfer of mannose from Dol-P-Man to Ser/Thr residues of secretory proteins. We identified and characterized five PMT subfamily genes, PMT1.1, PMT1.2, PMT2, PMT4 and PMT6 in the thermotolerant methylotrophic yeast Hansenula polymorpha. The hydropathy profile analysis predicts all H. polymorpha Pmt proteins to be integral membrane proteins with multiple transmembrane domains. For confirmation of subcellular localization of HpPmt proteins and characterization of PMT complexes, we constructed a set of HA or FLAG epitope-tagged versions of H. polymorpha Pmt proteins. All the C-terminal epitope-tagged HpPmt proteins were shown to be fully functional in vivo and to localize at the ER/Golgi membrane, except for Pmt6p. The significant sensitivity of Hppmt1.2 strain to the PMT1 inhibitor R3A-1c suggested that HpPMT1.2 might have a minor function redundant with HpPMT1.1. However, co-immunoprecipitation experiments using monoclonal anti-HA or anti-Flag bead revealed the complex formation between HpPmt1.1p and HpPmt2p, but no interaction between HpPmt1.2p and HpPmt2p even in the absence of HpPmt1.1p. The results strongly support the notion that interaction with HpPmt2p is required for the full mannosyltransferase activity of HpPmt1.1p, which plays a major role in O-mannosylation essential for cell wall integrity in H. polymorpha.
Kim, Jongsoon,Lee, Byungju,Kim, Hyungsub,Kim, Hyunah,Kang, Kisuk American Chemical Society 2016 Chemistry of materials Vol.28 No.19
<P>Defects in crystals such as antisites generally lead-to the deterioration of the ionic conductivity of solid-state ionic conductors. Herein, using first principles calculations, we demonstrate that the Li diffusion in Li2MP2O7 (M = Fe or Mn), a promising battery material, is sensitively affected by the presence of Li/M antisites; however, unexpectedly, the antisites significantly promote Li diffusion. The calculations reveal that the presence of antisites reduces the barrier of Li hopping and opens new paths for Li diffusion in the Li2MP2O7 crystal. In our experimental verification, we succeeded in synthesizing crystalline Li2MnP2O7 with varying Li/Mn antisite contents and demonstrated that the inclusion of antisites results in improved power capability with faster Li diffusion for Li-ion battery electrodes. We believe that this unexpected finding of increasing the ionic conductivity by introducing antisite defects broadens our understanding of solid-state ionic conductors and provides a new strategy for improving Li diffusion in conventional electrode materials for Li rechargeable batteries.</P>
Hyunah Kim,Hye Yun Moon,Dong-jik Lee,Seon Ah Cheon,Jeong-Nam Park,Hyun Ah Kang 한국당과학회 2013 한국당과학회 학술대회 Vol.2013 No.1
Protein O-mannosylation is evolutionarily conserved protein modification of fundamental importance, which is mediated by protein O-mannosyltransferases(Pmtproteins). The whole genome information of the thermotolerant methylotrophic yeast Hansenula polymorpha reveals the presence of five PMT homologues (HpPMT1, HpPMT2, HpPMT4, HpPMT5, and HpPMT6) encoding Pmt proteins, In this study, we carried out molecular characterization of HpPMT5 and HpPMT6, which show homologies to Saccharomyces cerevisiae PMT1 and PMT2 subfamily members, respectively. Although any detectable growth defects were not detected in the Hppmt5 and Hppmt6 single deletion mutants, they showed significantly increased sensitivity to the PMT1 inhibitor R3A-1c. As expected, the Hppmt1pmt5 and Hppmt1pmt6 double mutants became more susceptible to cell wall disturbing reagents, compared to the single Hppmt1 mutant. Furthermore, O-mannosylation of HpWsc1p and HpMid2p, the cell surface sensors of cell wall integrity-signaling, was significantly defected in the both Hppmt1pmt5 and Hppmt1pmt6 mutants. Interestingly, phosphorylaiton of Mpk1 protein, which results in the stimulation of the cell wall integrity pathway, was more markedly induced in the Hppmt1pmt5 than in Hppmt1 even under normal condition. The membrane fractionation experiment showed that all the HpPmt proteins are localized at the ER/Golgi membrane except HpPmt6p, which was mostly detected as soluble protein. By co-immunoprecipitation experiments, we confirmed the complex formation between HpPmt1p and HpPmt2p, but no interaction between HpPmt5p and HpPmt2p. Altogether, these results support that HpPmt5p and HpPmt6p have redundant functions to significantly compensate the loss of HpPmt1p in protein O-mannosylation that are essential for cell growth, cell wall integrity, and stress resistance of H. polymorpha.