화학적 발암화에 따른 Protein Kinase C의 발현 변화

변기정,홍락원,김진수 대한악안면성형재건외과학회 2001 Maxillofacial Plastic Reconstructive Surgery Vol.23 No.4

Protein kinase C (PKC) is known to play a pivotal role in neoplastic transformation cells and its high expression is often found in a variety of types of tumors including oral cancer. While PKC is associated with the altered signal transduction pathway of the tumor cells, it is still unclear which isoform is involved in the carcinogenesis process. Since the cellular distributions and the roles of PKC are isoform-specific, it is very important to identify the specific target molecules to improve our understanding of the carcinogenesis processes. Thus, the present study attempted to perform chemical carcinogen-induced neoplastic transformation of human epithelial cells and analyze the specific isoform of PKCs involved in the cellular transformation. The study analyzed overall PKC responses upon MNNG(N-Methyl-N'-nitro-N-nitroso guanidine) exposure with [3H] PDBu binding assay. PKC translocation was observed at high doses of MNNG treatment in the presence of extracellular calcium. Such effects were not observed in the absence of extracellular calcium. Translocational effects with exposure of MNNG was further enhanced in the presence of hydrocortisone. The result suggests that the type of PKC involved may be Ca2+-dependent classical isoform and steroid hormone enhances PKC activation. Among cPKC isoforms examined, only PKC-α and r showed significant translocation of protein levels from cytosolic fraction to membrane fraction, as analyzed by immunoblot. PKC-ε in nPKC class showed an inch·eased translocation, but other forms in this class did not show the effect. None of isoforms in aPKC class was affected by MNNG treat-ment. The study demonstrated that there was a certain specificity in the patterns of isoform induc-tion follwong chemical carcinogen exposure and helped identify all the types of PKC isoforms expressed in human epithelial cells. It was revealed that PKC isoforms were activated in an early resonse to chemical carcinogen, suggesting that PKC be associated with carcinogenesis process from an early stage in this particular cell system. The study will contribute to improving our understanding of chemical-induced carcinogenesis in human cells and may provide a scientific basis to introduce the specific PKC inhibitors as an anticancer drug of epithelial cell-origin cances including oral cancer.

구강 편평상피세포암 환자에서 치료전 혈청 CEA와 SCCA의 측정

장신남,김상규,김진수,변기정 大韓顎顔面成形再建外科學會 2003 Maxillofacial Plastic Reconstructive Surgery Vol.25 No.3

본 연구에서는 구강 편평상피세포암에서의 종양표지자의유용성을 평가하기위해 21명의 구강 편평상퍼세포암 환자들과 대조군인 22명의 양성질환자들에서 방사면역검사를 통해 치료전 혈청 CEA와 SCCA를 측정한 결과 다음과 같은 결론을 얻었다. 1. 양성질환자들의 혈청 CEA 평균치는 2.25±1.39ng/ml (최소 0.4 ng/ml), 최대 5.6 ng/ml)인 반면, 구강 편평상피세포암 환자들에서는 2.77±2.03 ng/ml (최소 0.3 ng/ml, 최대 6.7 ng/ml)로 두 군 사이에 통계적으로 유의한 차이를 나타내지 않았다(P= 0.3380) 2. 구강 편평상피세포암 환자들의 혈청 CEA의 양성률은 19.05%로 대조군인 양성질환자들의 양성률 4.55%와 통계적으로 유의한 차이를 보이지 않았다(P= 0.1853). 3. 양성질환자들의 혈청 SCCA 평균치는 1.21±1.74 ng/ml (최소 0.1 ng/ml, 최대 6.9 ng/ml)인 반면, 구강 편평상피세포암 환자들에서는 2.11±1.78 ng/ml (최소 0.1 ng/ml, 최대 6.1 ng/ml)로 두 군 사이에 통계적으로 유의한 차이를 나타내지 않았다(P= 0.1011) 4. 구강 편평상피세포암 환자들의 혈청 SCCA의 양성률은 47.62%로 대조군인 양성질환자들의 양성률 13.64%와 통계적으로 유의 한 차이를 나타내었다(P= 0.0153). 5. CEA와 SCCA 둘 중 하나라도 양성인 경우를 포함하는 조합 검사에서 민감도는 52.38%로 CEA의 민감도19.05% 보다는 크게 증가하였으나 SCCA의 민감도47.62%와 비교해서는 약간의 증가를 나타내었고, 특이도는 감소하였다 The study evaluated the usefulness of tumor markers in the diagnosis of oral sequamous cell carcinoma. Serum levels of two tumor markers(carcinoembryonic antigen〔CEA〕and squamous cell carcinoma antigen〔SCCA〕)were simultaneously measured by radiommunoassay in 21 patients with oral sequamous cell carcinoma and in 22 patients with oral benign diseases. The positive rates were 19.05% for CEA and 47.62% for SCCA in patients with oral sequamous cell carcinoma. The sensitivity of the combination assay(52.38%) was higher than that obstained with individual markers, but specificity was low in combination assay. Serum CEA seema to be inadequate for diagnostic purpose, but serum SCCA may be useful for diagnostic purpose in patients with oral sequamous cell carcinoma. A combination assay may be useful in the diagnosis of oral sequamous cell carcinoma.

Parapharyngeal Space에 발생한 Pleomorphic Adenoma의 치험례

장신남,백민주,김진수,변기정 大韓顎顔面成形再建外科學會 2003 Maxillofacial Plastic Reconstructive Surgery Vol.25 No.2

본 교실에서는 발생이 드문 부인두간극에 발생한 다형성 선종을 악하절개와 하악지 부위의 하악골 절단을 행한 이후에 둔적박리(blunt dissection)를 이용하여 종물과 주위의 구조물을 외과적으로 절제하였으며, 3년간의 추적 조사 결과 재발의 양상 없이 양호한 결과를 얻었기에 문헌고찰과 함께 보고하는 바이다. Pleomorphic adenoma is the most common of the benign salivary gland tumors. Pleomorphic adenoma accounts for 60% of all parotid gland tumors, 50% of submandibular gland tumors. and only 25% of sublingual gland neoplasms. Fifty percent of all oral minor gland tumors are pleomorphic adenomas of which 55% arise in the palate, 25% in the lip, 10% in the buccal mucosa, and 10% from all other oral and oropharyhgeal sites. The presence of tumors within the parapharyhgeal space is rare. Some investigators have reported pleomorphic adenomas originating in the deep lobe of the parotid gland advancing to involve this region, as well as primary pleomorphic adenomas of nonparotid origin. We present a case report of pleomorphic adenomas in the parapharyhgeal space with literature review. The tumor was completely removed under general anesthesia via submandibular incision with the division of mandible.

양측성 Warthin씨 종양 치험례

김상규,우길상,김진수,변기정 대한악안면성형재건외과학회 2002 Maxillofacial Plastic Reconstructive Surgery Vol.24 No.3

Warthin's tumor is a bunign neoplasm that occurs almost exclusively in the parotid gland. The tumor most frequently occurs in the tail of the parotid near the angle of the mandible. One unique feature of Warthin's tumor is the tendency of bilateral occurrence, which has been noted in 4 to 7 percent of cases. Most of these bilateral tumors do not occur simultaneously but are metachronous(occurring at different times). Histologically the tumor is composed of a mixture of ductal epithelium and a lymphoid stroma. Surgical removal is the treatment of choic for patients with Warthin's tumor. Warthin's tumor seldom recurs after removal. In our department, a 50-year old man, diagnosed as Warthin's tumor by fine needle aspiration biopsy, was treated by superficial parotidectomy on right side and subtotal parotidectomy on left side. For the treatment of Warthin's tumor, correct pretreatment diagnosis and the selection of appropriate surgical methods are important. We present a case report of the bilateral Warthin's tumor with the review of literatures.

법랑아세포종의 임상병리학적 연구

성일용,류성호,신상훈,김욱규,김종렬,정인교,변기정 大韓顎顔面成形再建外科學會 2003 Maxillofacial Plastic Reconstructive Surgery Vol.25 No.1

The authors analyzed retrospectively the 60 patients with ameloblastoma who were treated at Department of Oral and Maxillofacial Surgery, Pusan National University Hospital from MArch, 1990 to December, 1995. The results obtained were as follows: There were 26 males (43.3%) and 34 females (56.7%). The peak age of patients was the third decade (41.7%). The majority of cases occurred in the mandible 93.3%, especially in the angle region (91.1%). Eighteen of 60 patients (30.0%) were associated with an impacted tooth, by impacted 3rd molar teeth (16.6%), molar (10.0%), and premolar and canine (3.4%) in order., Fifteen of 60 patients (25%) were associated with an odontogenic cyst, by dentigenous cyst(13.3%), odontogenic keratocyst(8.4%), and radicular cyst(3.3%) in order. Radiographically, there were 32 unilocular types(54.2%) with average age of 24.9±10.8 years, 14 multilocular types(23.7%) with average age of 35.7±17.7 and 13 mixed types(22.0%) with average age of 41.4±15.8. Histopathologically, there were 42 follicular types(70.0%) with average age of 33.7±15.1 years and 18 plexiform types(30.0%) with average age of 26±14.6. Conservertive treatment was performed in the 33 patients(55.0%) and their average age was 25.2±13.9 years and radical treatment in the 27 patients(45.0%) and their average age was 39.3±41.8. Overall recurrence rate was 10%. A consistent correlation between the age of the patient and the radiographic or histologic type of mandibular ameloblastoma was observed. There was a tendency for ameloblastomas of the follicular type to show a multilocular or mixed appearance and for those of the plexiform type to show a unilocular appearance.

좌측 상하악 구치 후방부에 발생한 다형성 선종의 치험례

우길상,이은진,김상규,김진수,변기정 대한악안면성형재건외과학회 2002 Maxillofacial Plastic Reconstructive Surgery Vol.24 No.2

Pleomorphic adenoma is a circumscribed tumor characterized microscopically by its pleomorphic or mixed appearance, and its clearly recognizable epithelial tissue intermingled with areas of mucoid, myxoid, or chondroid appearance. Plemorphic adenoma, the most common tumor of the salivary glands, is now generally accepted as epithelial and benign adenoma. The palate is the most common site for minor salivary gland tumor. We experienced a case of pleomorphic adenoma occuring in the left retromolar pad area of unusual location. It is unusually large size so that the patient cannot close his mouth. The tumor occupied his mouth fully so that he has much difficulty in closing his mouth and breathing his breath. The lesion was treated with complete excision and reconstruced with buccal fat pedicle flap immediately on the operation under general anesthesia. Histopathological finding indicated pleomorphic adenoma on the left retromolar pad area.

하순 및 하악골 정중열의 치험례

차두원,김현수,백상흠,김진수,변기정 대한악안면성형재건외과학회 2001 Maxillofacial Plastic Reconstructive Surgery Vol.23 No.3

Median cleft of the lower lip and/or mandible is a rare congenital anomaly, first mentioned by Couronne in 1819. Monroe(1966), Fujino(1970), Ranta(1984) and Oostrom(1996) conducted comprehensive reviews and list cases in literature. Median cleft varies greatly, from a simple vermilion notch to a complete cleft of the lip involving the tongue, the chin, the mandible, the supporting structures of the median of the neck, and the manubrium sterni. The associated anomalies include ankyloglossia, cleft tongue, neck contraction, heart lesion, absence of hyoid bone, and so on. The etiology of median cleft is unknown. Various possibilities, such as failure of mesodermal penetration into the midline, failure of fusion of mandibular processes, external factors apart from the embryogenic pattern such as prensure, position in utero, circulatory failure caused placental adhesion, diseases in pregnancy, and so on, have been discussed. A 8-year-old girl was referred to the Dept. of Oral & Maxillofacial Surgery, Kyungpook National University Hospital and had been aware of the fact that at birth "she had something wrong with her mouth." Shortly after birth she had been examined by a plastic surgeon and at that time surgical procedure had been performed to release the tongue from the lower jaw and lip at local hospital. On admission, she had a slight notching of lower lip and two fibrous frenum ran from the lip along the ventral surface of the tongue, diastema between her mandibular central incisors, and slightly constricted bifid mandible associated independent movement of the two halves of mandible. The patient had autogenous iliac bone graft to reconstruct the mandibular midline defect. The postoperative result was uneventful. In future, the correction of the soft tissue deformities such as notching of the lower lip and partial ankyloglossia will be required for the esthetic and functional improvement.

Tensile Property Analysis of NCF Composite Laminated Structure for HP-CRTM Forming Process

Ki-Seok Byeon(변기석),Yu-Jeong Shin(신유정),Han-Kyu Jeung(정한규),Si-Woo Park(박시우),Chun-Su Roh(노춘수),Jin-Soo Je(제진수),Ki-Chul Kwon(권기철) 한국기계가공학회 2019 한국기계가공학회지 Vol.18 No.1

In recent years, the HP-CRTM method, which has the ability to produce carbon fiber-reinforce plastic composites at high speeds, has come into the spotlight in the automotive parts industry, which demands high productivity. Multi-axial carbon fabric, an intermediate material used in this HP-CRTM molding process, consists of layered fibers without crimp, which makes it better in terms of tensile and shear strength than the original woven fabrics. The NCF (non-crimp fabric) can form the layers of the carbon fiber, which have different longitudinal and lateral directions, and ±θ degrees, depending on the product’s properties. In this research, preforms were made with carbon fibers of ±45° and 0/90°, which were lamination structures under seven different conditions, in order to create the optimal laminated structure for automobile reinforcement center floor tunnels. Carbon fiber composites were created using each of the seven differently laminated preforms, and polyurethane was used as the base material. The specimens were manufactured in accordance with the ASTM D3039 standards, and the effect of the NCF lamination structure on the mechanical properties was confirmed by a tensile test.

흰쥐의 발치와 재상피화에 따른 상피내 CGRP 면역양성 신경섬유의 분포변화

변기정(Ki Jeong Byeon),김진수(Chin Soo Kim) 대한구강악안면외과학회 2000 대한구강악안면외과학회지 Vol.26 No.4

The purpose of this study was to investigate the distribution pattern of CGRP immunoreactive nerve fibers in the healing mucosa of extracion wound. Maxillary 1st molars of rats were extracted. All extraction sites and adjacent tissues of 3 groups of rats(1-week, 2-week and 4-week groups) were removed en bloc and processed for immunostaining and were subjected to light microscopic examination. The results obtained were as follows; In 1-week group, there was no difference in the distribution pattern of CGRP immunoreactive nerve fiber between epithelial margin adjacent to extraction socket and normal gingival epithelium. In 2-week group, some CGRP-immunoreactive nerve fibers were seen in epithelial layer. In 4-week group, many intercellular CGRP immunoreactive nerve fibers were abundant in all layers of immature epithelium characterized by scab on the mucosa and thick keratinized cell layer with irregular surface. Intraepithelial CGRP immunoreactive nerve fibers were reduced to normal level in adjacent mature epithelium. These results suggest that density of CGRP immunoreactive nerve fibers are increased transiently in epithelium during reepithelialization process and CGRP released from these nerve fibers may play an important role in the reepithelialization in the wound healing.

구강저에 발생한 유피낭종

변기정(Ki Jeong Byeon),이기호(Ki Ho Lee),김진수(Chin Soo Kim) 대한구강악안면외과학회 1999 대한구강악안면외과학회지 Vol.25 No.1

Dermoid cyst has evolved to represent any cyst filled with sebumlike material and with evidence of specialized skin derivatives from defective embryonic development. Approximately 7% of all dermoid cyst arise in the head and neck region and 0.01% of all oral cysts. The anterior region of the floor of the mouth is the most common site of occurrence in the oral cavity. There is no sex predilection and the age at presentation is usually in the second or third decades. Clinically, the lesions present as a rubbery or doughy mass that may vary in size from a few millimeters to 12 ㎝. An intraoral dermoid cyst usually presents as a slowly growing, painless swelling in the anterior floor of the mouth which may eventually cause elevation and displacement of the tongue resulting in dysphonia, dysphonia and dyspnea. Treatment involves surgical removal and recurrences have not been reported. We report three cases of dermoid cyst that were cared successfully in the department of dentistry, Kyung-pook National University. One case is a sublingual type, another case is a geniohyoid type, and the other is a lateral type. 저자등은 구강저에 발생한 3 증례의 유피낭종 및 유표피낭종을 악설골근을 기준으로 존재하는 위치에 따라 구내 및 구외접근법을 통한 외과적 적출을 시행하여 다른 합병증없이 양호한 결과를 얻었기에 보고하는 바이다.