<i>Asparagus densiflorus</i> in a vertical subsurface flow phytoreactor for treatment of real textile effluent: A lab to land approach for <i>in situ</i> soil remediation

Watharkar, Anuprita D.,Kadam, Suhas K.,Khandare, Rahul V.,Kolekar, Parag D.,Jeon, Byong-Hun,Jadhav, Jyoti P.,Govindwar, Sanjay P. Elsevier 2018 Ecotoxicology and environmental safety Vol.161 No.-

<P><B>Abstract</B></P> <P>This study explores the potential of <I>Asparagus densiflorus</I> to treat disperse Rubin GFL (RGFL) dye and a real textile effluent in constructed vertical subsurface flow (VSbF) phytoreactor; its field cultivation for soil remediation offers a real green and economic way of environmental management. <I>A. densiflorus</I> decolorized RGFL (40 gm L<SUP>−1</SUP>) up to 91% within 48 h. VSbF phytoreactor successfully reduced American dye manufacture institute (ADMI), BOD, COD, Total Dissolved Solids (TDS) and Total Suspended Solids (TSS) of real textile effluent by 65%, 61%, 66%, 48% and 66%, respectively within 6 d. Oxidoreductive enzymes such as laccase (138%), lignin peroxidase (129%), riboflavin reductase (111%) were significantly expressed during RGFL degradation in <I>A. densiflorus</I> roots, while effluent transformation caused noteworthy induction of enzymes like, tyrosinase (205%), laccase (178%), veratryl oxidase (52%). Based on enzyme activities, UV–vis spectroscopy, FTIR and GC-MS results; RGFL was proposed to be transformed to 4-amino-3- methylphenyl (hydroxy) oxoammonium and N, N-diethyl aniline. Anatomical study of the advanced root tissue of <I>A. densiflorus</I> exhibited the progressive dye accumulation and removal during phytoremediation. HepG2 cell line and phytotoxicity study demonstrated reduced toxicity of biotransformed RGFL and treated effluent by <I>A. densiflorus,</I> respectively. On field remediation study revealed a noteworthy removal (67%) from polluted soil within 30 d.</P> <P><B>Highlights</B></P> <P> <UL> <LI> <I>Asparagus densiflorus</I> showed potential to transform disperse dye Rubin GFL. </LI> <LI> Vertical subsurface flow phytoreactor efficiently decolorized real textile effluent. </LI> <LI> Toxicity study confirmed the reduced toxicity of biotransformed dye and effluent. </LI> <LI> <I>In situ</I> soil remediation studies revealed a noteworthy removal of soil ADMI. </LI> <LI> Lab to land transfer of phytoremediation technology was successfully achieved. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

Experimental investigation for the optimization of heat pipe performance in latent heat thermal storage

Chandrakishor Ladekar,S. K. Choudhary,S. S. Khandare 대한기계학회 2017 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.31 No.6

We investigated the optimum performance of heat pipe in Latent heat thermal energy storage (LHTES), and compared it with copper pipe. Classical plan of experimentation was used to optimize the parameters of heat pipe. Heat pipe fill ratio, evaporator section length to condenser section length ratio i.e., Heat pipe length ratio (HPLR) and heat pipe diameter, was the parameter used for optimization, as result of parametric analysis. Experiment with flow rate of 10 lit./min. was conducted for different fill ratio, HPLR and different diameter. Fill ratio of 80 %, HPLR of 0.9 and heat pipe with diameter of 18 mm showed better trend in charging and discharging. Comparison between the storage tank with optimized heat pipe and copper pipe showed almost 186 % improvement in charging and discharging time compared with the copper pipe embedded thermal storage. Heat transfer between Heat transferring fluid (HTF) and Phase change material (PCM) increased with increase in area of heat transferring media, but storage density of storage tank decreased. Storage tank with heat pipe embedded in place of copper pipe is a better option in terms of charging and discharging time as well heat storage capacity due to less heat lost. This justifies the better efficiency and effectiveness of storage tank with embedded optimized heat pipe.

<i>In situ</i> phytoremediation of dyes from textile wastewater using garden ornamental plants, effect on soil quality and plant growth

Chandanshive, Vishal V.,Kadam, Suhas K.,Khandare, Rahul V.,Kurade, Mayur B.,Jeon, Byong-Hun,Jadhav, Jyoti P.,Govindwar, Sanjay P. Elsevier 2018 CHEMOSPHERE - Vol.210 No.-

<P><B>Abstract</B></P> <P> <I>In situ</I> phytoremediation of dyes from textile wastewater was carried out in a high rate transpiration system ridges (91.4 m × 1.0 m) cultivated independently with <I>Tagetes patula, Aster amellus, Portulaca grandiflora and Gaillardia grandiflora</I> which reduced American Dye Manufacturers Institute color value by 59, 50, 46 and 73%, respectively within 30 d compared to dye accumulated in unplanted ridges. Significant increase in microbial count and electric conductivity of soil was observed during phytoremediation. Reduction in the contents of macro (N, P, K and C), micro (B, Cu, Fe and Mn) elements and heavy metals (Cd, As, Pb and Cr) was observed in the soil from planted ridges due to phyto-treatment. Root tissues of these plants showed significant increase in the specific activities of oxido-reductive enzymes such as lignin peroxidase, laccase, veratryl alcohol oxidase, tyrosinase and azo reductase during decolorization of textile dyes from soil. Anatomical studies of plants roots revealed the occurrence of textile dyes in tissues and subsequent degradation. A minor decrease in plant growth was also observed. Overall surveillance suggests that the use of garden ornamental plants on the ridges of constructed wetland for the treatment of dyes from wastewater along with the consortia of soil microbial flora is a wise and aesthetically pleasant strategy.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Ornamental plants at wetland ridges accumulated and degraded dyes from soil. </LI> <LI> Synergism between plants and microbes was involved in effective dye removal. </LI> <LI> Study revealed the entry and degradation of textile dyes in roots. </LI> <LI> Phytoremediation did not cause any severe toxicity on studied plants. </LI> <LI> The proposed treatment method was found to be aesthetically pleasant. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

HIV/AIDS-related lymphoma: perspective from a regional cancer center in India

A.H. Rudresha,Pravin Ashok Khandare,D. Lokanatha,Abraham Jacob Linu,M.C. Suresh Babu,K.N. Lokesh,L.K. Rajeev,Carol Saldanha Smitha,Vaibhav Baburao Amale,C.S. Premalata,Mulchandani Nikita 대한혈액학회 2019 Blood Research Vol.54 No.3

BackgroundIndia has the third largest population of people living with HIV/AIDS (PLHA). Lymphoma is the second most common malignancy among PLHA. However, data are lacking regard-ing HIV/AIDS-related lymphoma (ARL) in India. This study evaluated the epidemiology and clinical outcomes of ARL from a regional cancer center in India.MethodsThis retrospective analysis included cases of ARL between March 2011 and September 2017. Data were obtained from patient record files for the assessment of epidemiology and clinical outcomes. Statistical analysis was performed using GraphPad Prism 6. Comparisons of subtype-specific survivals were performed using log-rank tests.ResultsOf 1,226 lymphoma cases, 80 (6.5%) were ARL. Details were available for 70 patients. The median age at diagnosis was 40.5 (9‒74) years with a male:female ratio of 2:1. AIDS-defining lymphomas (ADL) constituted 78.6% of cases, while 21.4% had non-AIDS defining lymphoma (NADL). The mean CD4 counts were 193.15±92.85 and 301.93±107.95 cells/L, respectively (t-test; P=0.0002). Extranodal involvement was present in 55.7%, B symptoms were reported in 60%, and lactate dehydrogenase (LDH) was elevated in 64.3% of patients. The median overall survival times were 6 months for plasmablastic lymphoma (PBL), 23 months for diffuse large B-cell lymphoma (DLBCL), and was not reached for Hodgkin’s lymphoma (log-rank test; P=0.0011). Other histo-logical subtype cases were too few to draw meaningful survival outcomes.ConclusionARL is a heterogeneous disease. Histologic subtype is a major determinant of the clinical outcome. ADL has significantly lower CD4 counts than those of NADL. There is an urgent and unmet need for uniform management guidelines for improving outcomes in this un-der-represented patient population.

Densitometric quantification for the validation of decolorization of Disperse Orange ERL by lichen <i>Parmelia</i> sp.

Kulkarni, Ashwini N.,Bhalkar, Bhumika N.,Khandare, Rahul V.,Kurade, Mayur B.,Jeon, Byong-Hun,Govindwar, Sanjay P. Society for Bioscience and Bioengineering, Japan 2019 Journal of bioscience and bioengineering Vol. No.

<P>Densitometric high performance thin layer chromatography (HPTLC) quantification method was developed to validate the decolorization/biotransformation of Disperse Orange ERL and dye mixture by lichen <I>Parmelia</I> sp. which release several colored compounds during decolorization process, hence unable to use colorimetric estimation. Percent decolorization of Disperse Orange ERL and dye mixture by lichen <I>Parmelia</I> sp. was observed when estimated using developed HPTLC method. Limit of detection and limit of quantification for both dyes in mixture were obtained as 0.3 and 1 μg/μl, respectively. Area of peak of control Disperse Orange ERL was reduced by 43% after 12 h, 71% after 48 h and upto 82% after 72 h of incubation. Precision and repeatability of data elucidated the % relative standard deviation less than 3 for all the values thus indicating statistically acceptable. Biodegradation of dye and mixture was confirmed with Fourier transform infrared spectroscopy analysis, i.e., altered fingerprinting spectral pattern.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Densitometric quantification of textile dyes biotransformation products. </LI> <LI> Quantification of dye in presence of released colored compounds. </LI> <LI> Reported for the first time in dye decolorization studies. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

Co-planted floating phyto-bed along with microbial fuel cell for enhanced textile effluent treatment

Kadam, Suhas K.,Watharkar, Anuprita D.,Chandanshive, Vishal V.,Khandare, Rahul V.,Jeon, Byong-Hun,Jadhav, Jyoti P.,Govindwar, Sanjay P. Elsevier 2018 JOURNAL OF CLEANER PRODUCTION Vol.203 No.-

<P><B>Abstract</B></P> <P>A floating phytobed system based on plants of <I>Chrysopogon zizanioides</I> and <I>Typha angustifolia</I> (Consortium CT) was effective in the removal of Scarlet RR Dye (150 mg/L) and a textile effluent, with rates of 89 and 87%, respectively, within a 60-h period, which demonstrates a higher elimination rate than an individual plantation. In addition, the treatment of textile effluents with the floating phytobed linked to microbial fuel cells was enhanced in terms of color reduction, chemical oxygen demand, biological oxygen demand, total dissolved solids and total suspended solids up to 82, 75, 75, 67 and 70%, respectively. Moreover, it produced a power of 0.0769 W/m<SUP>2</SUP> at current density of 0.3846 A/m<SUP>2</SUP>. Terminal restriction length polymorphism community analysis documented 37 new genera which have a probable role in efficient treatment as well as power generation. Induction in the activities of oxidoreductase, high performance thin layer chromatography and gas chromatography-mass spectroscopy analyses of treated Scarlet RR dye confirmed the biotransformation. Toxicity evaluated on gill histology of <I>Lamellidens marginalis</I> and inter simple sequence repeat marker assessment confirmed the decreased toxicity of Scarlet RR after phyto-transformation.</P> <P><B>Highlights</B></P> <P> <UL> <LI> <I>Chrysopogon zizanioides</I> and <I>Typha angustifolia</I> treated Scarlet RR and real effluent. </LI> <LI> Co-planted phyto-bed (FPb) gave efficient dye removal with energy generation. </LI> <LI> Removal of dyes improved note-worthily by FPb-Microbial Fuel Cells system. </LI> <LI> Degradation pathway of Scarlet RR by co-plantation system was proposed. </LI> <LI> Toxicity study on bivalve revealed less toxic nature of dye products. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

Phytobeds with <i>Fimbristylis dichotoma</i> and <i>Ammannia baccifera</i> for treatment of real textile effluent: An <i>in situ</i> treatment, anatomical studies and toxicity evaluation

Kadam, Suhas K.,Chandanshive, Vishal V.,Rane, Niraj R.,Patil, Swapnil M.,Gholave, Avinash R.,Khandare, Rahul V.,Bhosale, Amrut R.,Jeon, Byong-Hun,Govindwar, Sanjay P. Elsevier 2018 Environmental research Vol.160 No.-

<P><B>Abstract</B></P> <P> <I>Fimbristylis dichotoma, Ammannia baccifera</I> and their co-plantation consortium FA independently degraded Methyl Orange, simulated dye mixture and real textile effluent. Wild plants of <I>F. dichotoma</I> and <I>A. baccifera</I> with equal biomass showed 91% and 89% decolorization of Methyl Orange within 60h at a concentration of 50ppm, while 95% dye removal was achieved by consortium FA within 48h. Floating phyto-beds with co-plantation (<I>F. dichotoma</I> and <I>A. baccifera</I>) for the treatment of real textile effluent in a constructed wetland was observed to be more efficient and achieved 79%, 72%, 77%, 66% and 56% reductions in ADMI color value, COD, BOD, TDS and TSS of textile effluent, respectively. HPTLC, GC-MS, FTIR, UV–vis spectroscopy and activated oxido-reductive enzyme activities confirmed the phytotrasformation of parent dye in to new metabolites. T-RFLP analysis of rhizospheric bacteria of <I>F. dichotoma</I>, <I>A. baccifera</I> and consortium FA revealed the presence of 88, 98 and 223 genera which could have been involved in dye removal. Toxicity evaluation of products formed after phytotransformation of Methyl Orange by consortium FA on bivalves <I>Lamellidens marginalis</I> revealed less damage of the gills architecture when analyzed histologically. Toxicity measurement by Random Amplification of Polymorphic DNA (RAPD) technique revealed bivalve DNA banding pattern in treated Methyl Orange sample suggesting less toxic nature of phytotransformed dye products.</P> <P><B>Highlights</B></P> <P> <UL> <LI> <I>F. dichotoma</I> L. and <I>A. baccifera</I> L. decolorized Methyl Orange and real textile dye effluent. </LI> <LI> Co-plantation of <I>F. dichotoma</I> L. and <I>A. baccifera</I> L. gave more efficient dye removal. </LI> <LI> Possible degradation pathways of Methyl Orange by all three systems are proposed. </LI> <LI> Effluents were treated note-worthily in floating phyto-beds by plants. </LI> <LI> Toxicity study on bivalve revealed less toxic nature of dye products. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

Synthesis and characterization of indium-borate glassceramic containing Eu0.01Ce0.74 Zr0.25O1.995 via an incorporation method

Abdolali Alemi,Leila Kafi-Ahmadi,Ali Akbar Khandar,Amin Salem 한양대학교 세라믹연구소 2011 Journal of Ceramic Processing Research Vol.12 No.6

Glass in the system 5In2O3.94Na2B4O7 was fabricated via a melt quenching technique. The amorphous nature of the quenched glass was confirmed by X-ray powder diffraction. The infrared spectrum shows no boroxol ring formation in the structure of this glass. Differential thermal analysis shows a glass transition temperature of 696 oC and a crystallization temperature of 1151 oC. A cerium–zirconium mixed oxide Ce0.75Zr0.25O2 and Eu-doped cerium-zirconium mixed oxide were obtained by a solid-state method. Then the glass powder and Eu-doped cerium-zirconium mixed oxide were mixed. The mixture was heated in a crucible. The glass-ceramic sample was obtained by pouring the melts onto stainless steel plates. The samples obtained were annealed at 450 oC for 1 h to remove the thermal strain. Gas phase catalytic oxidation of toluene was studied over these nanostructures. The results showed that the catalytic activity of Eu0.01Ce0.74Zr0.25O1.995 was higher than Ce0.75Zr0.25O2 for conversion of toluene. Differential thermal analysis for a glass-ceramic sample shows a glass transition temperature of 648 oC and a crystallization temperature of 1134 oC. A scanning electron microscopy study of the glass-ceramic indicates that the crystallized glass consists of crystals with an average diameter of about 86 nm dispersed in glassy regions.