PCR-Restricition Fragment Length Polymorphism 방법에 의한 Borrelia burgdorferi Sensu Lato의 분류

송혜원,김홍,박상욱,엄용빈,김종배,박성언,김근희 THE KOREAN SOCIETY FOR BIOMEDICAL LABORATORY SCINE 1999 Journal of biomedical laboratory sciences Vol.5 No.2

라임병의 원인균인 Borrelia burgdorferi에 대하여 각 균종의 표준균주와 진드기에서 추출한 DNA를 template로 PCR을 실시한 후 그 증폭산물을 Alu I으로 처리한 restriction fragment length polymorphism (RFLP) 방법으로 각 균종의 연관성을 조사하고자 하였다. 표준균주로 RFLP를 실시한 결과 B. burgdorferi sensu stricto와 B. garinii의 RFLP 형태 (50 bp, 70 bp, 150 bp)가 유사하였으며 B.afzelii에서는 다른 RFLP형태 (50bp, 110bp, 150 bp)를 관찰하였다. 그 중 B. afzelii KK-1과 B. garinii HP1은 새로운 RFLP 형태를 보여 B. afzelii와 B. garinii는 각각 2 types의 subgroup으로 분류할 수 있었다. 진드기 DNA에서 는 B. afzelii를 포함한 각 균종에 대하여 모두 유사한 RFLP 형태를 보였는데, 진드기 DNA에서 확인된 B. afzelii는 KK-1과 같은 군에 속하는 것으로 사료되었다. For the classification of B. burgdorferi sensu lato strains, PCR-restriction fragment length polymorphism (RFLP) analysis was performed. PCR was carried out with B. burgdorferi sensu lato specific primer set (BB uni set), and amplicons of 470-bp DNA were digested with Alu I. The Alu I restriction polymorphism of the amplicons provided a useful tool for identifying B. burgdorferi sensu lato strains. Both amplicons from B. burgdorferi sensu stricto and B. garinii except HP1 strain showed identical RFLP pattern (50 bp, 70 bp, and 150 bp), but amplicons from B. afzelii and B. garinii showed two types of subgroups, respectively. The result of PCR-RFLP using extracted DNAs from ticks was similar to those patterns of B. burgdorferi species including B. afzelii.

Hypofractionated radiation therapy for head and neck cancers in the era of intensity-modulated radiation therapy

Keun-Yong Eom(Keun-Yong Eom) 대한방사선종양학회 2024 Radiation Oncology Journal Vol.42 No.1

Is neoadjuvant androgen deprivation therapy beneficial in prostate cancer treated with definitive radiotherapy?

Keun-Yong Eom,MD,Sung W Ha,MD,Eunsik Lee,MD,Cheol Kwak,MD,Sang Eun Lee,MD 대한방사선종양학회 2014 Radiation Oncology Journal Vol.32 No.4

Purpose: To determine whether neoadjuvant androgen deprivation therapy (NADT) improves clinical outcomes in patients with prostate cancer treated with definitive radiotherapy. Materials and Methods: We retrospectively reviewed medical records of 201 patients with prostate cancer treated with radiotherapy between January 1991 and December 2008. Of these, 156 patients with more than 3 years of follow-up were the subjects of this study. The median duration of follow-up was 91.2 months. NADT was given in 103 patients (66%) with median duration of 3.3 months (range, 1.0 to 7.7 months). Radiation dose was escalated gradually from 64 Gy to 81 Gy using intensity-modulated radiotherapy technique. Results: Biochemical relapse-free survival (BCRFS) and overall survival (OS) of all patients were 72.6% and 90.7% at 5 years, respectively. BCRFS and OS of NADT group were 79.5% and 89.8% at 5 years and those of radiotherapy alone group were 58.8% and 92.3% at 5 years, respectively. Risk group (p = 0.010) and radiation dose ≥70 Gy (p = 0.017) affected BCRFS independently. NADT was a significant prognostic factor in univariate analysis, but not in multivariate analysis (p = 0.073). Radiation dose ≥70 Gy was only an independent factor for OS (p = 0.007; hazard ratio, 0.261; 95% confidence interval, 0.071–0.963). Conclusion: NADT prior to definitive radiotherapy did not result in significant benefit in terms of BCRFS and OS. NADT should not be performed routinely in the era of dose-escalated radiotherapy.

Is neoadjuvant androgen deprivation therapy beneficial in prostate cancer treated with definitive radiotherapy?

Eom, Keun-Yong,Ha, Sung W.,Lee, Eunsik,Kwak, Cheol,Lee, Sang Eun The Korean Society for Radiation Oncology 2014 Radiation Oncology Journal Vol.32 No.4

Purpose: To determine whether neoadjuvant androgen deprivation therapy (NADT) improves clinical outcomes in patients with prostate cancer treated with definitive radiotherapy. Materials and Methods: We retrospectively reviewed medical records of 201 patients with prostate cancer treated with radiotherapy between January 1991 and December 2008. Of these, 156 patients with more than 3 years of follow-up were the subjects of this study. The median duration of follow-up was 91.2 months. NADT was given in 103 patients (66%) with median duration of 3.3 months (range, 1.0 to 7.7 months). Radiation dose was escalated gradually from 64 Gy to 81 Gy using intensity-modulated radiotherapy technique. Results: Biochemical relapse-free survival (BCRFS) and overall survival (OS) of all patients were 72.6% and 90.7% at 5 years, respectively. BCRFS and OS of NADT group were 79.5% and 89.8% at 5 years and those of radiotherapy alone group were 58.8% and 92.3% at 5 years, respectively. Risk group (p = 0.010) and radiation dose ${\geq}70Gy$ (p = 0.017) affected BCRFS independently. NADT was a significant prognostic factor in univariate analysis, but not in multivariate analysis (p = 0.073). Radiation dose ${\geq}70Gy$ was only an independent factor for OS (p = 0.007; hazard ratio, 0.261; 95% confidence interval, 0.071-0.963). Conclusion: NADT prior to definitive radiotherapy did not result in significant benefit in terms of BCRFS and OS. NADT should not be performed routinely in the era of dose-escalated radiotherapy.

Invasion of the great vessels or atrium predicts worse prognosis in thymic carcinoma

Keun-Yong Eom,Hak Jae Kim,Hong-Gyun Wu,Young Tae Kim,Dae Seog Heo,Young Whan Kim 대한방사선종양학회 2013 Radiation Oncology Journal Vol.31 No.3

Purpose: We evaluated treatment outcomes of thymic carcinomas to determine prognostic factors for survival. Materials and Methods: Between May 1988 and May 2009, 41 patients had pathologic diagnosis of thymic carcinoma in Seoul National University Hospital, Seoul, Korea. Of these, 40 patients were followed up to 188 months after treatment. The mean age of all patients was 58.3 years and male to female ratio was 23 to 17. Results: Among 30 patients who underwent surgical resection, 26 achieved R0 resection and postoperative radiotherapy (PORT) was performed in 22 patients (73%). Various chemotherapeutic regimens were given with local treatment modalities, surgery and/or radiotherapy, in 12 patients. The 5-year locoregional control (LRC), distant metastasis-free survival, progression-free survival (PFS), and overall survival were 79.4%, 53.0%, 42.6%, and 63.6%, respectively. Patients with Masaoka stage I or II showed excellent prognosis of 5-year PFS around 90%. In advanced stages, invasion of the great vessels or atrium by thymic carcinomas was negative prognostic factor for PFS in univariate analysis. Lymph node involvement was statistically significant factor for LRC and PFS. Local or regional recurrence was infrequent after surgical resection followed by PORT, while distant metastasis was the major component of treatment failure. Conclusion: Complete resection followed by PORT provided remarkable local control without severe acute toxicities in patients with stage II and favorable stage III thymic carcinoma. Invasion of the great vessels or atrium was statistically significant prognostic factor for PFS.

Postoperative Radiotherapy for Parotid Gland Malignancy

Keun-Yong Eom(엄근용),Hong-Gyun Wu(우홍균),Jae-Sung Kim(김재성),Charn Il Park(박찬일),In-Ah Kim(김인아),Kwang Hyun Kim(김광현),Chae Seo Lee(이재서) 대한방사선종양학회 2005 Radiation Oncology Journal Vol.23 No.3

목 적: 귀밑샘 악성종양의 수술 후 방사선 치료 성적 및 예후인자를 분석하였다. 대상 및 방법: 1980년부터 2002년까지 130명의 귀밑샘 악성 종양 환자가 서울대병원 방사선 종양학과 데이터베이스에 등록되었고 이 중 수술 후 방사선 치료를 받은 72명의 환자에 대하여 후향적 분석을 실시하였다. 남자는 42명, 여자는 30명이었고, 나이의 중앙값은 46.5세였다. 점액표피양암종이 가장 흔한 조직학적 유형이었다. 병기에 따라서는 I, I, III, IV 병기에 각각 6, 23, 23, 20명이 해당되었다. 수술 부위의 방사선 조사량의 중앙값은 60 Gy였고, 일회 조사량의 중앙값은 1.8 Gy였다. 결 과: 5년 전체 생존율 및 10년 전체 생존율은 각각 85%와 76%였다. 5년 국소제어율은 85%였고 6년이 지나면서 변화가 없었다. 다변량분석을 하였을 때, 성별, 조직학적 유형이 생존율과 통계적 유의성이 있었다. 나이, 안면신경마비증상, 병기(T 병기, N병기, 병기)는 생존율과 관련된 통계적 유의성이 없었다. 국소제어율의 경우, 림프절 전이 및 절제연 침범여부가 의미있는 예후인자였다. 조직학적 유형, 종양의 크기, 신경침윤여부 및 수술의 종류는 국소제어율의 예후인자가 아니었다. 결 론: 귀밑샘 악성종양에서 수술 후 방사선치료의 우수한 성적을 확인하였다. 성별 및 조직학적 유형이 생존율에 대한 의미있는 예후인자였고, 림프절 전이 및 절제연의 침윤이 있는 경우 국소제어율이 불량했다. Purpose: The aim of this study was to evaluate the clinical results of postoperative radiotherapy for parotid gland malignancy, and determine prognostic factors for locoregional control and survival. Materials and Methods: Between 1980 and 2002, 130 patients with parotid malignancy were registered in the database of the Department of Radiation Oncology, Seoul National University Hospital. The subjects of this analysis were the 72 of these 130 patients who underwent postoperative irradiation. There were 42 males and 30 females, with a median age of 46.5 years. The most common histological type was a mucoepidermoid carcinoma. There were 6, 23, 23 and 20 patients in Stages I, II, III and IV, respectively. The median dose to the tumor bed was 60 Gy, with a median fraction size of 1.8 Gy. Results: The overall 5 and 10 year survival rates were 85 and 76%, respectively. The five-year locoregional control rate was 85%, which reached a plateau phase after 6 years. Sex and histological type were found to be statistically significant for overall survival from a multivariate analysis. No other factors, including age, facial nerve palsy and stage, were related to overall survival. For locoregional control, nodal involvement and positive resection margin were associated with poor local control. Histological type, tumor size, perineural invasion and type of surgery were not significant for locoregional control. Conclusion: A high survival rate of parotid gland malignancies, with surgery and postoperative radiotherapy, was confirmed. Sex and histological type were significant prognostic factors for overall survival. Nodal involvement and a positive resection margin were associated with poor locoregional control.

Invasion of the great vessels or atrium predicts worse prognosis in thymic carcinoma

Eom, Keun-Yong,Kim, Hak Jae,Wu, Hong-Gyun,Kim, Young Tae,Heo, Dae Seog,Kim, Young Whan The Korean Society for Radiation Oncology 2013 Radiation Oncology Journal Vol.31 No.3

Purpose: We evaluated treatment outcomes of thymic carcinomas to determine prognostic factors for survival. Materials and Methods: Between May 1988 and May 2009, 41 patients had pathologic diagnosis of thymic carcinoma in Seoul National University Hospital, Seoul, Korea. Of these, 40 patients were followed up to 188 months after treatment. The mean age of all patients was 58.3 years and male to female ratio was 23 to 17. Results: Among 30 patients who underwent surgical resection, 26 achieved R0 resection and postoperative radiotherapy (PORT) was performed in 22 patients (73%). Various chemotherapeutic regimens were given with local treatment modalities, surgery and/or radiotherapy, in 12 patients. The 5-year locoregional control (LRC), distant metastasis-free survival, progression-free survival (PFS), and overall survival were 79.4%, 53.0%, 42.6%, and 63.6%, respectively. Patients with Masaoka stage I or II showed excellent prognosis of 5-year PFS around 90%. In advanced stages, invasion of the great vessels or atrium by thymic carcinomas was negative prognostic factor for PFS in univariate analysis. Lymph node involvement was statistically significant factor for LRC and PFS. Local or regional recurrence was infrequent after surgical resection followed by PORT, while distant metastasis was the major component of treatment failure. Conclusion: Complete resection followed by PORT provided remarkable local control without severe acute toxicities in patients with stage II and favorable stage III thymic carcinoma. Invasion of the great vessels or atrium was statistically significant prognostic factor for PFS.

교육환경이 아파트 가격에 미치는 영향

엄근용 ( Keun Yong Eom ),윤충한 ( Choong Han Yoon ),임덕호 ( Duk Ho Lim ) 한국경제통상학회 (구 한국경상학회, 한국국민경제학회) 2006 경제연구 Vol.24 No.4

MC50 싸이클로트론에서 생성되는 중성자선의 생물학적 특성의 평가

엄근용(Keun-Yong Eom),우홍균(Hong-Gyun Wu),박혜진(Hye Jin Park),허순녕(Soon Nyung Huh),예성준(Sung-Joan Ye),이동한(Dong Han Lee),박석원(Suk Won Park) 대한방사선종양학회 2006 Radiation Oncology Journal Vol.24 No.4

목 적: 원자력의학원에 설치되어 있는 MC밍 사이클로트론에서 생성되는 중성자선의 의학적 이용을 위하여 생물학적 특성을 평가하고자 하였다. 대상 및 방법: 35 MeV 양성자를 15 mm 베릴륨 표적에 부딪혀서 생성된 줄성자선에 대하여 물리적 방사선선량을 측정한 후 체외실힘 (in-vitro) 을 하였다 EMT-6 세포주 (cell line) 를 이용하여 0-5 Gy의 중성자선을 조사 후 생존분획 (surviving fraction)을 구하였다 또한 감마선의 효과를 피하기 위하여 납차페를 한 후에 동일 조건에서 생존분획을 구하였다. 엑스선 실험에서는 0, 2, 5, 10, 15 Gy를 조사 후 생존분획을 측정하였다. 결 과: MC5O 의 중성자선은 조사야 26x26 cm2, 전류 10μA, 갚이 2 cm에서 84%의 중성자와 16%의 감마선으로 구성되어 있었고, 총선량률은 9.25 cGy/minOI 었다 엑스선을 이용하여 측정한 생존분획곡선은 선형이차함수모델(linear quadratic mode l)을 적용하였을 때 a/ /3 비는 0.611 (a=O.0204, /3=0.0334, R2~0.999) 이었다 중성자선에 있어서 생존분획곡선은 저선량 영역에서 어깨영Qoj (shoulder area) 을 가지고 있었고, 모든 실험에서 선형이차함수모델에 잘맞았다 Q 으| 평균값은 -0.315 (범우I , -0.254- -0.360)였고,/3값은 0.247 (범우 I , 0.220-0 .262) 이었다. 납차폐를 하였을 때에도 생존분획곧선에서 어깨영역은 없어지지 않았다 중성자선으 1 RBE (relative biological effectiveness) 값은 생존분획이 0.1 일 때 2.07-2.19 빔위였고, 0.01 일 때 2.2 1-2.35였다. 결 론: MC50에서 생성된 중성자선은 상당량의 감마선을 내포하고 있으며 이것이 생존분획곡선에서 어깨영역이 나타나는 데에 기여하였을 것이다. MC50의 중성자선의 RBE 값은 약 2.2였다. Purpose: To evaluate biological characteristics of neutron beam generated by MC50 cyclotron located in the Korea lnstilule of Radiological and Medical Sciences (KIRAMS) Materials and Methods: The neutron beams generated with 15 mm Berylium target hit by 35 MeV proton beam was used and dosimetry data was measured before in-vitro study. We irradiated 0, 1, 2, 3, 4 and 5 Gy of neutron beam to EMT-6 cell line and surving fraction (SF) was measured. The SF CUNe was also examined at the same dose when applying lead shielding to avoid gamma ray component. In the X-ray experiment, SF CUNe was obtained after irradiation at 0, 2, 5, tO, and 15 Gy Resuns: The neutron beams have 84% of neutron and 16% of gamma cαnponent at the depth of 2 cm with the field size of 26 x 26 em', beam current 20 μA , and dose rate of 9.25 cGy/min. The SF CUNe from X-ray, when fitted to linear-quadratic (LQ) model, had 0.61 t as al l3 ratio (a o O.0204, 130 0.0334, R'oO.999, respectlveψ). The SF CUNe from neutron beam had shoulders at low dose area and fitted well to LQ model with the value of R' exceeding 0.99 In all experiments. The mean value of alpha and beta were -0.315 (range, -0.25 4- -0.3601 and 0.247 (0.220-0.262), respectively. The addition of lead shielding resulted in no straightening of SF CUNe and shoulders in low dose area still existed. The RBE of neutron beam was in range 이 2.07-2.19 with SFoO.l and 2.21-2.35 with SFoO.Ol , respectiveψ conclusion: The neutron beam from MC50 cyclotron has significant amount of gamma component and this may have contributed to form the shoulder of survival CUNeo The RBE of neutron beam generated by MC50 was about 2.2

지역별 주택경기지수 작성에 관한 연구

엄근용(Eom, Keun-Yong),진창하(Jin, Chang-Ha) 대한국토·도시계획학회 2018 國土計劃 Vol.53 No.3