김치유래 젖산균의 균체지방산 분석을 이용한 분류학적 연구

이정숙,정민철,김우식,이근철,김홍중,박찬선,이헌주,주윤정,이근종,안종석,박완,박용하,민태익 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 1996 한국미생물·생명공학회지 Vol.24 No.2

표준균주를 포함한 230여개의 김치유래 젖산균에 대한 균체지방산(FAMEs)을 분석하였다. FAMEs profiles는 Euclidian Distance 17.5에 의해 7개의 Major Cluster와 1개의 Single Cluster로 나뉘어졌다. 이중 A, B, C 및 Cluster는 Leuconostoc속으로 분석되어졌고, F는 Lactobacillus속으로 분석되어졌다. 그리고 E와 G cluster는 두개의 Genus가 혼재되어 나타났으며 보충적인 연구가 필요하다. 앞으로 김치유래 젖산균의 균체지방산 분석결과를 기반으로 한 데이타베이스에 95가지 탄소원을 이용하는 수치분류학적 접근방법 및 Pyrolysis Mass Spectrometry 등의 화학적 분석 방법과 분자친화적 연구를 통한 종합적 분류정보 체계가 갖추어지면 젖산균의 신속, 정확한 동정 및 연구에 활발히 이용되어질 것이다. Two hundreds and thirty lactic acid bacteria, mostly isolated from Kimchi, including type strains were sued for analysis of cellular fatty acids. The 230 test strains were recoverd in 7 major and 1 single clusters defined a Euclidian distance of 17.5. These aggregate taxa were equivalent to the genus Leuconostoc (aggregate group A, B, C and D), and the genera Leuconostoc and Lactobacillus (aggregate group G). It is concluded as evident that FAMEs (Fatty Acid Methyl Esters) profile of cell can be used as a criterion in classification of lactic acid bacterial from kimchi. Additional comparative taxonomic studies need to be carried out on well chosen representative strains to determine the most appropriate methods of value.

의사결정지원시스템에서 직관적이고 사용자 친숙한 모델 해결을 위한 모델과 솔버의 유연한 통합에 대한 연구

이근우,허순영 한국경영과학회 2005 韓國經營科學會誌 Vol.30 No.1

Research in the decision sciences has continued to develop a variety of mathematical models as well as software tools supporting corporate decision-making. Yet, in spite of their potential usefulness, the models are little used in real-world decision making since the model solution processes are too complex for ordinary users to get accustomed. This paper proposes an intelligent and flexible model-solver integration framework that enables the suer to solve decision problems using multiple models and solvers without having precise knowledge of the model-solution processes. Specifically, for intuitive model-solution, the framework enables a decision support system to suggest the compatible solvers of a model autonomously without direct user intervention and to solve the model by matching the model and solver parameters intelligently without any serious conflicts. Thus, the framework would improve the productivity of institutional model solving tasks by relieving the user from the burden of learning model and solver semantics requiring considerable time and efforts.

논에 있어서 포장정보 공간변이의 공간통계학적 해석

이충근,손연규,성제훈,정인규,김상철,박우풍,박원규 한국국제농업개발학회 2002 韓國國際農業開發學會誌 Vol.14 No.2

논에 있어서 정밀농업을 위한 기초자료수집과 분석을 하기 위해서 토양의 특성, 포장면 고저차, 생육상태 그리고 수량에 관한 포장정보를 조사한 결과 아래와 같은 결과를 얻었다. 가. 기본통계값을 살펴보면 유효태 규산의 경우는 한 포장내 14배 이상의 변이차이를 보였으며, 생육정보 중 분얼수는 3배, 수량은 4배 이상의 차이를 보였다. 나. 포장정보의 변이계수를 살펴보면 5.45∼51.3%의 공간변이를 보였다. 토양특성 중에 pH를 제외한 나머지가 10%이상의 공간변이를 보였다. 포장면 고저차는 51.3%, 생육정보는 7.32∼23.2%, 그리고 수량에 있어서도 변이계수가 22.5%를 보였다. 다. 포장정보를 공간통계학을 이용해 해석한 결과, 포장정보의 공간구조 발달여부를 표시하는 Q값이 0.24∼1로서 공간구조가 포장정보에 따라서 발달되어 있었으며, 공간변이의 의존거리를 나타내는 랜지는 8.1∼147.9m를 보였다. 그러나, 실질적인 랜지는 토양특성값이 15∼50m 정도, 생육정보는 15m 전후, 포장면 고저차는 30m 정도, 수량은 8.1m를 보였다. 이것을 기초로 하여 크리깅 방법으로 데이터를 보간하여 지도화 시킨결과, 공간변이를 이해하는데 유용하게 이용될 수 있는 포장정보 지도를 얻을 수 있었다. Soil properties, relief of field surface, growth information, grain yield were investigated in a 1ha paddy field in 2001 to obtain basic field information for precision agriculture. The field information were analyzed to examine their within field variability using descriptive statistical method. Semivariograms and Kriged maps of geostatistical analysis were also adopted to examine their within field spatial dependence. The results obtained were as follows ; 1) Nutrient distribution difference of available SiO_2 was seemed 14 times overs, 3 times overs at tiller number, 4 times overs at grain yield from within a paddy field. 2) Descriptive statistics of field information showed that the coefficient of variation ranged from 5.45∼51.3%. 3) Field information showed a high spatial dependence within a paddy field. The Q values ranged from 0.24∼1, the ranges of spatial dependence were from 8.1∼50m, respectively. 4) Kriged maps enable the visualization and comparison of the spatial variability of field informaton.

Ehrlich 복수암 세포의 지질대사에 관한 연구

이희성,하창우,이근배 중앙대학교 의과대학 의과학연구소 1977 中央醫大誌 Vol.2 No.1

We have studied on lipids of Ehrlich ascites tumor cells (EATC). Lipids were extracted by Kennedy and Kanfer(1964) method and lipoproteins were separated by paper electrophoresis. For the identification and assay of lipid extracts, thin-layer chromatography was employed. The incorporation of ^32P-orthophosphate into phospholipids of EATC was also observed. The results obtained were summarized as follows; 1. The total lipid content of EATC was 47.15 ㎎ per g wet weight; of this 32.2㎎ per g was neutral lipid and 14.95 ㎎ per g was phospholipid. 2. The amounts of neutral lipid fractions of EATC, i.e., free cholesterol, esterified cholesterol, mono-, di-, triglyceride and free fatty acid were 2.06㎎, 1.75㎎, 4.71㎎, 7.15㎎, 10.74㎎ and 2.21㎎, respectively. The amount of triglyceride of EATC was similar to those of normal mouse liver. 3. Phospholipid composition of EATC was phosphatidylcholine, phosphatidylethanolamine, phosphatidylinositol, phosphatidylserine and unidentified phospholipid, and contents of the individual phospholipids showed that the over-all composition were very similar to normal mouse liver. 4. Lipoprotein fractions of EATC were chylomicron, β-lipoprotein, α-lipoprotein, and prealbumin. Chylomicron fraction contained a larger amount of lipid and α-lipoprotein fraction contained a smaller amount of lipid. 5. The incorporation of ^32P into phospholipids reached a maximum 5 minutes after incubation.

부산광역시의 대기질 변화경향 및 특성에 관한 연구

이화운,김유근,원경미,홍정혜,문윤섭,장난심,정우식 부산대학교 환경문제연구소 1997 環境硏究報 Vol.15 No.1

Holstein種의 血液化學値에 關한 硏究 : -Ⅰ. 成牝牛의 血液化學値- -Ⅰ. Blood Chemical Values of Adult Cows -

李載洪,林貞澤,韓邦根,金宇權 全南大學校 農漁村開發硏究所 1981 農業科學技術硏究 Vol.16 No.1

國內에서 飼育되고 있는 Holstein種成牝牛의 血液化學値를 얻고자 全南地域에서 飼育되고 있는 外觀上 健康하다고 認定된 經産牛 385頭를 對象으로 血液化學値를 調査檢討하여 다음과 같은 結論을 얻었다. 1. 平均血淸TP量은 7.35(5.10∼10.20)g/100㎖이었으며 年令의 增加에 따라서 漸漸 증가하는 傾向이 있었다. 2. 平均血淸Alb量은 3.51(2.50∼4.40)g/100㎖이었으며 年令別 差異는 認定할수 없었다. 3. 平均血淸Glb量은 3.84(1.40∼7.20)g/100㎖이었으며 年令의 增加에 따라서 漸漸 증가하는 傾向이 있었다. 4. 平均血淸 A/G比는 0.91(0.43∼2.64)이었으며 年令의 增加에 따라서 漸漸 증가하는 傾向이 있었다. 5. 平均血淸 cholesterol量은 179.38(46.4∼336.0)g/100㎖이었으며 年令의 增加에 따라 增加하여 4年牛를 頂点으로 다시 下向의 傾向을 나타내고 있었다. 6. 平均血淸 glucose量은 39.38(27.0∼57.3)㎎/100㎖로 낮았으며 年令別로 特徵的인 것은 發見할수 없었다. 7. 平均血淸 magnesium量은 2.72(0.20∼8.46)㎎/100㎖이었으며 老令牛에서 magnesium의 減少가 顯著하였다. 8. 平均血淸 calcium量은 8.69(6.34∼16.83)㎎/100㎖로 全般的으로 낮은 량이었으며 年令別 差異는 認定할수 없었다. 9. 平均血淸無機燐量은 5.88(3.50∼9.76)㎎/100㎖이었으며 年令別로 特徵的인 것은 發見할수 없었다. 10. 平均血淸Ca/P比는 1.48(0.68∼2.64)이었으며 年令別로 特徵的인 것은 發見할수 없었다. 11. 平均血淸 potassium量은 18.95(13.69∼24.24)㎎/100㎖이었으며 年令別 差異는 認定할수 없었다. 12. 平均血淸 sodium量은 315.90(282.86∼361.05)10.20)㎎/100㎖이었으며 年令別로 特徵的인 것은 發見할수 없었다. 13. 平均血淸 chloride量은 354.64(299.97∼361.05)㎎/100㎖이었으며 年令別 差異点은 發見할수 없었다. The present study was conducted to determine the blood chemical values of adult Holstein cows in Korea. Samples of blood were taken from 385 healthy cows in Chonnam province, and the results obtained are summarized as follows. 1. Mean serum totalprotein content was 7.35(5.10∼10.20)g/100㎖;it in creased with age. 2. Mean serum albumin content was 3.51(1.40∼4.40)g/100㎖;no differences were found between age groups in the serum albumin content. 3. Mean serum globulin content was 3.84(1.40∼7.20)g/100㎖; it increased with age. 4. Mean serum albumin/globulin ratio was 0.91(0.43∼2.64);it decreased with age. 5. Mean serum glucose concentration was 179.38(46.4∼336.0)g/100㎖;it increased up to 4 years of age and thereafter it decreased with age. 6. Mean serum glucose concentration was 39.38(27.0∼57.3)㎎/100㎖, which was low compared with other results. No differences were found in the glucose concentration between age groups. 7. Mean serum magnesium concentration was 2.72(0.20∼8.46)㎎/100㎖;it decreased significantly in older cows. 8. Mean serum calcium concentration was 8.69(6.34∼16.83)㎎/100㎖, which was low compared with other results. No differences were found in the calcium concentrati on between age groups. 9. Mean serum inorganic phosphate concentration was 5.88(3.50∼9.76)㎎/100㎖;no differences were found in the inorganic phosphate concentration between age groups. 10. Mean serum calcium/phosphate(Ca/P) ratio was 1.48(0.68∼2.64);no differences were found in the Ca/P ratio between age groups. 11. Mean serum potassium concentration was 18.95(13.69∼24.24)㎎/100㎖;no differences were found in the potassium concentration between age groups. 12. Mean serum sodium concentration was 315.90(282.86∼361.05)10.20)㎎/100㎖;no differences were found in the sodium concentration between age groups. 13. Mean serum chloride concentration was 354.64(299.97∼361.05)㎎/100㎖;no differences were found in the cholride concentration between age groups.

Honeycomb 觸媒에 의한 窒素酸化物과 2-CP의 同時除去에 관한 硏究

李宇根 江原大學校 附設 環境硏究所 1993 環境硏究 Vol.10 No.-

도시폐기물 소각로에서 배출되는 질소산화물과 Dioxin類를 동시에 제거하기 위해서 honeycomb型 觸媒를 이용하여 반응온도 200-400℃의 범위에서 질소산화물과 Dioxin類의 前?物質인 2-CP의 同時除去 실험을 수행하였다. 실험결과에 따르면 space velocity 6000 hr^(-1)과 NH₃/NO_(x)比 0.5에서 90%이상의 NO_(x)제거율이 얻어졌으며 space velocity 1200 hr^(-1)에 2-CP가 거의 완전히 분해됨을 알 수 있었다. 동시제거 실험에서는 공존물질에 의한 제거율의 저하는 없었고 2-CP의 경우 단독 제거보다 동시제거 실험에서의 제거율이 더 높음을 알 수 있었다. The removal of NO_(x) and 2-CP was investigated over the temperature range of 200-400℃ by using a honeycomb catalyst, for the purpose of finding the condition to decompose simultaneously NO_(x) and dioxin. The removal efficiency of NO_(x) was 90% at the space velocity of 6,000 hr^(-1) and NH₃/NO_(x) ratio of 0.5. And the removal efficiency of 2-DP was 99% at the space velocity of 1200 hr^(-1). There was no effect in the removal efficiency of NO_(x) and 2-CP by co-existing materials(NO_(x), NH₃, 2-CP etc.) at the simultaneous decomposition. The removal efficiency of 2-CP in the simultaneous decomposition was higher than in the only decomposition of 2-CP.

체육교사의 정신지체학생 지도유형에 대한 하위구조의 신뢰성과 타당성 검증

이현수,이근모,손승우 釜山大學校 附設 體育科學硏究所 2005 體育科學硏究所 論文集 Vol.21 No.2

The purpose of this study was to reliability and validity of the substructure about physical education teachers teaching types to Students with Mental Retradation. The study used to 241 middle school students(male:118, female:123) as subjects for actual analysis with the purposive sampling, except insincere 11 samples......

인체 Calbindin-D9k 항체 제작과 종특이적 활성

이근우, 홍의주, 이근식, Vu-Hoang Dang, 정의배 충북대학교 동물의학연구소 2006 Journal of Biomedical and Translational Research Vol.7 No.2

본문참고

비산재 중에 함유된 중금속의 용출특성에 관한 연구(1)

이우근,김진범,김은미 江原大學校 附設 環境硏究所 1997 環境硏究 Vol.14 No.-

The secondary environmental contamination is now feared by the land-fill treatment os fly ash from MSWI. It is urgently necessary to study the development of a proper technology for fly ash. considering the governmental plan for enhancing the rate of incineration. The leaching characteristics of MSWI ash were analyzed for pH, L/S ratio and leaching time in our study. According to the result, pH was decreased as the leaching time elapsed. The amounts of leached heavy metal were affected by L/S ratio. pH is the most important among the factors for affecting the amounts. Therefore, the leaching experiment must be excuted under the pseudo-actual condition(actual pH at the land-fill site)evaluating the risk.