Phanerochaete chrysosporium의 액체 배양 및 Lignin Peroxidase 생산

박세근,정명선,김영관 江原大學校 産業技術硏究所 2001 産業技術硏究 Vol.21 No.A

This study characterizes the growth of white rot fungi Phanerochaete chrysosporium IFO 31249 and lignin peroxidase(LiP) activity in different submerged culture media. P. chrysosporium was grown in the form of pellet of various sizes from a spore inoculum under shaking liquid culture condition. While the growth of mycelia was higher under the nitrogen-sufficient culture than under the nitrogen-limited culture, ligninase activity was relatively lower. The lignin peroxidase appeared in nitrogen-limited culture and was suppressed by excess nitrogen. High level(40U/l) of lignin peroxidase activity was obtained in the growth medium containing 1.5mM veratryl alcohol, a secondary metabolite of P. chrysosporium. Lignin peroxidase production was not observed under conditions of nitrogen sufficiency or in balanced media, suggesting that control parameters could increase the activity by manipulating the secondary metabolism.

수도관의 생물막 형성에 미치는 잔류염소와 파이프 재질의 영향

박세근,박재우,성권식,최성찬,김영관 江原大學校 産業技術硏究所 2001 産業技術硏究 Vol.21 No.B

This laboratory study examined the impact of free chlorine residual and pipe material on the formation of biofilm in drinking water distribution pipe surfaces. Result of heterotrophic plate counts(HPC) of the biofilm in the tap water- supplied reactor averaged 2.17×105CFU/cm2 on PVC and 2.43×105CFU/cm2 on STS 316, respectively. HPCs on the surface exposed to the tap water containing 0.2mg/L of free chlorinne residual averaged 4.24×104CFU/cm2 on PVC and 6.54×104CFU/cm2 on STS 316, respectively Average of HPC/Total direct counts in the tap water-supplied reactor ranged from 1.08%(PVC) to 1.26%(STS 316) and from 0.38%(PVC) to 0.65%(STS 316) in the reactor supplemented with disinfectant, resfectively. No correlation was observed between disinfectant addition and biofilm density. With regard to the biofilm formation, little difference existed between PVC and STS 316. Yellow and red pigmented bacteria were the dominant expressions in bulk fluid, whereas non-pigmented bacteria were found dominant in the biofilm. Pink/red pigmented bacteria were found to be facultative anaerobic, while yellow pigmented bacteria and non-pigmented bacteria were found to be obligate aerobic.

원발성 조기 위 소세포암

송근영,강길,박정희,김경종,민영돈,박치영,임성철 朝鮮大學校 附設 醫學硏究所 2002 The Medical Journal of Chosun University Vol.27 No.2

Gastric small cell carcinoma(SCC) is defined as poorly-differentiated neuroendocrine tumor of the stomach, and their morphologic and biologic features arc similar to those of the lung. It is known that this tumor has poor prognosis even in early pathologic stage, It acounts for only 3% of all gastrointestinal SCC. that has been reported only 33 cases outside and 6 cases inside of the comitiy. Most of these reports are related to advanced gastric SCC. as pathologic stage IIIb or IV. This report describes a case of early gastric SCC. pathologic stage Ib.

하수슬러지의 유동층 소각 특성

한근희,박영성,류정인 대전대학교 산업기술연구소 1999 산업기술연구소 論文集 Vol.10 No.2

The incineration characteristics of sludge sample were investigated in a fluidized bed incinerator(φ 0.2m, 3.0m height). The sewage sludge with heating value of 2480kcal/kg, and 22% moisture content was used as sludge sample. The characteristics of sludge incineration in terms of combustion efficiency, axial temperature profile and heavy metal analysis of sludge and ash were studied. The combustion efficiencies were found to be no less than 99%, which indicates that the fluidized bed incinerator can be very effectively applied to sewage sludges. It was also found that the heavy metal components were remained in the sludge ash after incineration due to the relatively low combustion temperature of the fluidized bed incinerator.

SO₂와 NOx 동시제거를 위한 흡수제의 반응특성

이형근,최원길,박영성 大田大學校 環境問題硏究所 1997 환경문제연구소 논문집 Vol.2 No.-

Recently the more stringent emission standards of government promulgate the incentive for developing the technology capable of simultaneous removal of SO₂ and NOx from flue gas. This study was to develope an enhanced absorbent in reactivity and adsorption ability for removing SO₂and NOx from flue gas. The characteristics of spray drying absorption(SDA) process and status of SDA process were studied, as well as analysis of properties of absorbent and its manufacturing method. CaO, MgO, and NaHCO₃were selected as a alkali reagent, and they were mixed with fly ash(FA) or activated carbon powder(AC) and their the reactivity and adsorption ability toward SO₂and NOx were greatly enhanced through got water curing process. Among the highest. The surface area of MgO mixed with FA(30%) was greater than NaHCO₃, but the SO₂and NOx removal performance of MgO mixed with FA(30%) was much lower than that of NaHCO₃. The removal quantity of SO₂and NOx per mole of NaHCO₃mixed with FA(30%) was 2.7 mole, this meant that the effect of physical adsorption was greater than chemical reaction between absorbent and acid gases.

방사선 조사후 아크릴릭 레진의 물성변화

이용근,이건일,박영준,변호영 원광대학교 생체재료·매식연구소 1995 원광생체재료·매식 Vol.4 No.2

To investigate the effect of high dose irradiation on the mechanical properties. dental acrylic resin specimens were irradiated and the Vickers hardness number(VHN) and the 3-point flexure strength(FS) were measured before and after irradiation. Five kinds of acrylic resins were studied. Two(VRS. PS2) were heat cured denture base resins. one(VSC) was chemically cured denture base resin and the other two(ORJ, VOP) were orthodontic resins. The acrylic resin specimens were made according to the manufacturer's instructions and were finally polished with Microcloth (Buehler). Among ten specimens of each material. five specimens were irraditated with 100 Gy using a linear accelerator (Mitsubishi 6 MV), and the others were not. The specimens were immersed in 37℃ D.W. for 48 hours before testing. Each specimens the VHN was measured 10 times. Their FS was measured with an universal testing machine (Instron 4302, England), with a cross-head speed of 5 mm/min. The VHNs of heat cured resins were 21.52∼25.55 kg/㎟ before irradiation and 19.93∼26.58 kg/㎟ after irradiation. Those of chemically cured resins were 11.28∼16.6kg/㎟ before irradiation and 11.3∼14.3 kg/㎟ after irradiation. The VHNs of heat cured acrylic resins were significantly higher than those of chemically cured resins were significantly higher than those of chemically cured resins before and after irradiation(p<0.05). VHN of VRS was significantly increased after irradiation, and those of VOP and PS2 were significantly decreased after irradiation (p<0.05). The FS of resins studied were 6.7∼8.0 kg/㎟ before irradiation and 6.5∼8.4 kg/㎟ after irradiation. The FS of VSC and VRS increased after irradiation and those of VOP, ORJ and PS2 decreased after irradiation, but the results were not statistically significant. Before irradiation, the FS of heat cured resins were somewhat higher than those of chemically cured resin, but not statistically significant, and after irradiation the FS of heat cured resins were significantly higher than those of orthodontic resins. In conclusion. high dose irradiation did not result in consistent changes in mechanical properties.

유동층내 설치된 수평관의 마모 특성

한근희,박영성,류정인 대전대학교 산업기술연구소 1998 산업기술연구소 論文集 Vol.9 No.2

유동층내에 설치된 수평관에 대한 마모실험을 수행하였다. 유동층 연소로의 크기는 단면적 0.15×0.2m이며 높이는 1m에 해당된다. 유동층내 유동물질로는 모래와 무연탄회재를 사용하였으며 충진층의 높이는 0.45m를 적용하였다. 마모실험용 시편은 아크릴 관이 사용되었으며 위치는 수직방향으로 15, 30, 45cm 위치에 세 개씩 그리고 60cm 위치에 1개를 설치하였다. 유동화속도, 수직높이, 층물질 종류에 따른 마모속도와 관의 원주방향에 대한 마모특성을 고찰하였다. 실험결과 마모속도는 유동화속도와 관의 수직높이가 증가할수록 늘어남을 보였으며, 시계방향으로 4∼8시간, 특히 5∼7시간 방향으로 커다란 마모율을 나타냈다. The erosion characteristics of horizontal tubes immersed in a fluidized bed cold mode combustor were investigated. The combustor has a cross sectional area of 0.15×0.2m and a height of 1m. The anthracite ash and river sand were used as a bed material. The static bed height was 0.45m. The wear specimens were made of acrylic tube. Test tubes were located inside the bed with three tubes in a low at 15, 30, 45cm and with a single tube at 60cm above distributor plate, respectively. The test was performed to investigate the effect of fluidization velocity, distance above distributor plate on the erosion rate, and the erosion trend of tube surface with radial direction. The result shows that the erosion rate increases with fluidization velocity, with distance above the distributor plate. The trend with radial direction shows severe erosion at the 4-8 o'clock clockwise compare to other area, especially 5 and 7 o'clock clockwise.

인슐린 비의존성 당뇨병에 동반된 심상성 천포창 1 례

이상준,박진용,임태형,윤영호,권순대,남창욱,허규찬,박근용,구본식,김상표,장종억 啓明大學校 醫科大學 1997 계명의대학술지 Vol.16 No.3

저자들은 인슐린 비의존형 당뇨병 환자에서 통증성 구강내 미란 및 전신성 수포성 미란을 동반한 심상성 천포창 1예를 경험하였기에 문헌고찰과 함께 보고하는 바이다. Various skin conditions such as diabetic dermopathy, necrobiosis lipoidica diabeticorum, bullosis diabeticorum, granuloma annulare occur frequently in diabetes, although common lesions may be associated by chance. Pemphigus vulgaris(PV) is a blistering skin disease seen predominantly in elderly patients. This disorder is characterized by the loss of cohesion between epidermal cells with the resultant formation of intraepidermal blisters. These blisters rupture easily, leaving denuded areases that may crust and enlarge peripherally. In half or more of patients, lesions begin in the mouth: approximately 90% of patients have oromucosal involvement at some time during the course of their disease. But the relationship of this disorder with diabetes is not clear. We experienced a case of pemphigus vulgaris associated with NIDDM in 67 years old woman who was admitted due to painful oral ulcer and control of blood glucose, so we present this case with a review of literatures.

교정용 선재와 브라켓 사이의 마찰력에 관한 연구

김철위,박영준,이용근 大韓齒科器材學會 1995 대한치과재료학회지 Vol.22 No.2

Mechanical friction makes the orthodontic tooth movement require greater force than the biological tooth movement. Friction is affected by the size, shape, and material of both the bracket and the wire. This study was designed to investigate the effect of material or size of orthodontic brackets and orthodontic wires on the amount of frictional forces generated during simulated tooth movement. A testing apparatus was designed to simulate the clinical situation in which the center of resistance of a tooth is not on the same plane as that of bracket, thereby resulting in some tipping of the bracket slot relative to orthodontic wire (100 gram weight was suspended). In angulated and non-angulated experimental condition, the orthodontic wires were drawn through the testing apparatus with the speed of 2mm/minute. The results were as follows : 1. In both of the cases of the metal bracket and ceramic bracket, the frictional forces were greater in angulated experimental group than non-angulated group. In both of the non-angulated and angulated experimental group, the frictional forces of ceramic brackets were greater than those of metal brackets. In most of cases, these differences were statistically significant(p<0.05) with a few exceptions. 2. In general, the frictional forces of orthodontic wires with circular cross-cut surface were lower than those wires with rectangular cross-cut surfaces. But there was no statistically significant correlation between the size of orthodontic wire and frictional forces. 3. There was no difference between the frictional of the stainless-steel orthodontic wires and those of the cobalt-chromium orthodontic wires, but the frictional forces varied depending on the measuring condition or brand of the wires. 4. The frictional forces of β-titanium orthodontic wires were significantly higher than those of stainless-steel orthodontic wires were cobalt-chromium orthodontic wires.

소나무(Pinus densiflora) 목질을 이용한 수용액 중의 Cu(II)흡착

김하나,박세근,양경민,김영관 강원대학교 산업기술연구소 2007 産業技術硏究 Vol.27 No.A

Milled Korean pine (Pinus densiflora) wood was used to evaluate its adsorption capacity of Cu(Ⅱ) ions from aqueous solution by running a series of batch experiments. Prior to the tests, the milled woods were pretreated with 1N NaOH, 1N HNO_(3), and distilled water, respectively, to examine the effect of pretreatment. Within the tested pH range between 3 and 6, copper adsorption efficiency of NaOH-treated wood(96~99%) was superior to the HNO_(3)-treated wood(19~31%) and distilled water-treated wood(18~35%). The efficiency of copper removal by wood enhanced with increasing solution pH and reached a maximum copper ion uptake at pH 5~6. Adsorption behavior of copper onto both raw and HNO_(3)-treated woods was mainly attributed to interaction with carboxylic acid group. For NaOH-treated wood, carboxylate ion produced by hydrolysis or saponification was a major functional group responsible for Cu sorption. NaOH treatment of wood changed the ester and carboxylic acid groups into carboxylate group, whereas HNO_(3) treatment did not affect the production of functional groups which could bind copper. A pseudo second-order kinetic model fitted well for the sorption of copper ion onto NaOH-treated wood. A batch isotherm test using NaOH-treated wood showed that equilibrium sorption data were better represented by the Langmuir model than the Freundlich model.