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Sung Hee Lee,Keon-Sang Chae,Dong Hwan Sohn 대한약학회 2004 Archives of Pharmacal Research Vol.27 No.4
Expressed sequence tags (ESTs) were generated from two 3'-directed cDNA libraries constructed from quiescent and activated rat hepatic stellate cell (HSC) to analyze the expression profiles of active genes in both cells. From quiescent and activated HSC, 694 ESTs and 779 ESTs, respectively, were obtained after excluding those having shorter than 30 bp. Among ESTs obtained from quiescent and activated HSC, 68 and 73 kinds of ESTs (186 clones and 236 clones), respectively, appeared more than once, implying that their genes are expressed highly in each cell type. 52 among 73 ESTs appeared only in the activated HSC, 47 among 68 ESTs only in the normal HSC, and 21 in both cells. The genes of these 52 ESTs were assumed to be expressed more highly in the activated HSC. To confirm the high expression of genes of which the ESTs appeared more than twice in the activated HSC, northern hybridization was carried out with RNAs derived from rat normal and fibrotic liver using each of 18 EST DNAs as probe. 13 ESTs showed more intense bands with RNA isolated from the fibrotic liver than normal liver. From these results, we confirm the positive correlation between abundance of transcript in activated HSCs and the expression level in fibrotic liver. The expression profile of the transcripts serves as an important tool in understanding the biological properties of HSC.
Sang-Chae Jeon,Keon Sik Kim,Dong-Joo Kim,Dong Seok Kim,Jong Hun Kim,Jihae Yoon,Jae Ho Yang 한국방사성폐기물학회 2019 방사성폐기물학회지 Vol.17 No.1
사고저항성 핵연료의 일환으로 UO2 입자가 세라믹 셀 벽으로 둘러싸인 미세구조를 갖는 세라믹 미소셀 UO2 소결체를 개발 중이다. 이는 핵분열생성물들을 UO2 펠렛 내에 포집하여 펠렛 외부로의 방출을 저감함으로써 봉내압 상승을 완화하고 응력부식균열 발생률을 낮춘다. 생성량이나 방사능 측면에서 위험한 핵분열생성물 중 하나로 여겨지는 세슘은 세라믹 미소셀 소결체 내에서 셀 물질과 화학반응 하여 포집될 수 있다. 따라서, 세슘 포집능은 해당 화학반응의 열역학적, 속도론적 특성에 의해 결정된다. 역으로, 미소셀 소결체의 조성설계 시 해당 반응에 대한 열역학적 예측이 필수적이다. 본 연구는 세라믹 현재 개발 중인 여러 미소셀 조성(Si-Ti-O, Si-Cr-O, Si-Al-O)에 대해 세슘의 포집능을 평가하는 열역학적 계산을 다룬다. 계 산에 앞서 먼저 HSC Chemistry를 이용해 세슘과 셀 물질의 물리/화학적 상태를 정의한 후, LWR 정상운전 모사환경에서 계 산된 세슘포텐셜(ΔGCs)과 산소포텐셜(ΔG02)에 근거하여 세슘포집 반응성을 평가하였다. 계산 결과에 근거하면, 세슘 포집 반응은 상기 모든 조성에서 자발적일 것으로 예상되며 이로써 조성설계의 근거를 제시함과 동시에 세슘의 포집능을 평가하는 효과적인 방법을 제공한다. As candidates for accident-tolerant fuels, ceramic microcell fuels, which are distinguished by their peculiar microstructures, are being developed; these fuels have UO2 grains surrounded by cell walls. They contribute to nuclear fuel safety by retention of fission products within the UO2 pellet, reducing rod pressure and incidence of SCC failure. Cesium, a hazardous fission product in terms of amount and radioactivity, can be captured by chemical reactions with ceramic cell materials. The capture-ability of cesium therefore depends on the thermodynamics of the capturing reaction. Conversely, compositional design of cell materials should be based on thermodynamic predictions. This study proposes thermodynamic calculations to evaluate the cesium capture-ability of three ceramic microcell compositions: Si-Ti-O, Si-Cr-O and Si-Al-O. Prior to the calculations, the chemical and physical states of the cesium and the cell materials were defined. Then, the reactivity was evaluated by calculating the cesium potential (ΔGCs) and oxygen potential (ΔG02) under simulated LWR circumstances of normal operation. Based on the results, cesium capture is expected to be spontaneous in all cell compositions, providing a basis for the compositional design of ceramic microcell fuels as well as a facile way for evaluating cesium capture.
Chromosomal Assignment of Short cDNA Sequences by PCR Using Overlapping and Tailed Short Primers
CHAE, Keon-Sang,MURAKAWA, Katsuji,OKUBO, Kousaku,MATSUBARA, Kenichi 全北大學校 基礎科學硏究所 1994 基礎科學 Vol.17 No.-
Overlapping primers and tailed short primers are effective agents for mapping very short cDNA sequences. By using such primers, human cDNAs as short as 32 nucleotides in length can produce PCR bands. Using these and other primers of ordinary size, 44 cDNAs were assigned to chromosomes, of which 24 were assigned to single chromosomes, and 2 were assigned to two chromosomes and two were assigned to three chromosomes, respectively. Among the 24 cDNAs, all of which matched GenBank entries, 6 cDNAs were observed to map to the same chromosomes as reported previously.
Chae, Keon-Sang,Katsuji Murakawa,Kousaku Okubo,Kenichi Mastsubara 全北大學校 基礎科學硏究所 1994 基礎科學 Vol.17 No.-
For the PCR-based chromosomal assignment of very short cDNA fragments specifically designed primers are required. We tested primers with very short core sequences that are identical or complementary to known cDNA sequences, with or without tails at the 5' ends. The lower limit of the core length for PCR using human chromosome templates was 14 nucleotides (nt) when they have tails. The minimal length of the tail was 2 nt when it was attached to the 5' end of a 14-nt core. In the absence of a tail. 15 nt are needed for the core to act properly. The overall size of the short cDNA fragments that could be assigned was further reduced by using a pair of primers that overlap at the 3' ends. The limits of the free energy of overlap were about -1.9 kcal mol at 45 C, -2.9 ㎉/mol at 50 C and -4.5 ㎉/mol at 55 C. A combination of these features in a primer pair allowed cDNA fragments as short as 30 nt to be assigned.
퍼지추론시스템 기반 지중송전계통 보호용 거리계전 알고리즘 개발
鄭彩均(Chae-Kyun Jung),李鍾範(Jong-Beom Lee),吳聖權(Sung-Kwun Oh),朴建俊(Keon-Jun Park),李在奎(Jae-Kyu Lee) 대한전기학회 2008 전기학회논문지 Vol.57 No.2
If the fault occurs on the underground power cable systems, the fault current on the sheath has an influence on all sections of cable because it's returned through earth at the directly grounded point and operation point of SVL(Sheath Voltage Limiter) on each insulated joint box. Therefore, the earth resistance and the operation of SVL have an effect on the zero-sequence current, and then the impedance between relaying point and fault point is increased. That causes the overreach of distance relay. For these reasons, the distance relay algorithm for protecting an underground power cable systems hasn't been developed till now. In this paper, new distance relay algorithm is developed for protecting a underground power cable system using fuzzy inference system which is the one of ACI(Advanced Computational Intelligence) techniques. This algorithm is verified by EMTP simulation of real power cable system, and proves to effectively advance the errors
정채균(Chae-Kyun Jung),박흥석(Hung-Sok Park),강지원(Ji-Won Kang),장태인(Tai-in Jang),윤종건(Jong-Keon Yoon),김양상(Yang-Sang Kim),석광현(Kwang-Hyun Suk),김형호(Hyung-Ho Kim) 대한전기학회 2009 대한전기학회 학술대회 논문집 Vol.2009 No.7
This paper describes surge characteristics on 345㎸ combined transmission system mixed overhead line and GIL(Gas Insulated Transmission Line). Then, it is compared with the results of the system mixed overhead line and XLPE power cables. Finally the lightning overvoltages are also discussed according to surge arrester installation in two kinds of combined transmission systems when the lighting strikes on the phase conductor.
정채균(Chae-Kyun Jung),강지원(Ji-Won Kang),윤종건(Jong-Keon Yoon),김양상(Yang-Sang Kim) 대한전기학회 2009 전기학회논문지 Vol.58 No.9
In a previous paper, the characteristics of ECC (earth continuity conductor) have been analysed for reducing the level of induced sheath voltage considering the dimension and position of ECC, the spacing between ECC and three phase cables, and the use of two ECC conductors at the single point boned section of underground power cable system. From these results, the study conditions for optimal installation has been selected such as installation section, conductor size and etc. In this paper, 5 cases which are set by possible installation conditions are tested based on previous research results. Finally, the optimal installation method of ECC is selected on underground power cable systems.