http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
- 中文 을 입력하시려면 zhongwen을 입력하시고 space를누르시면됩니다.
- 北京 을 입력하시려면 beijing을 입력하시고 space를 누르시면 됩니다.
RISS 인기검색어
- 검색키워드
- 저자 : Keith W. Singletary (검색결과 2 건)
- 무료
- 기관 내 무료
- 유료
-
Anthocyanin-Rich Grape Extract Blocks Breast Cell DNA Damage
Keith W. Singletary,Kwan-Jae Jung,Monica Giusti 한국식품영양과학회 2007 Journal of medicinal food Vol.10 No.2
Anthocyanins, belonging to the flavonoid family of phytochemicals, have received attention as agents that mayhave potential in preventing chronic diseases such as cardiovascular diseases and certain cancers. In the present study, an an-thocyanin-rich extract from Concord grapes [referred to as Concord grape extract (CGE)] and the anthocyanin delphinidinwere evaluated for their capacity to inhibit DNA adduct formation due to the environmental carcinogen benzo[a]pyrene (BP)in MCF-10F cells, a noncancerous, immortalized human breast epithelial cell line. CGE at 10 and 20 .g/mL and delphinidinat 0.6 .M concentrations significantly inhibited BPDNA adduct formation. This was associated with a significant increasein activities of the phase II detoxification enzymes glutathione S-transferase and NAD(P)H:quinone reductase 1. In addition,these grape components also suppressed reactive oxygen species (ROS) formation, but did not induce antioxidant responseelement-dependent transcription. Taken together, these data suggest that CGE and a component grape anthocyanin have breastcancer chemopreventive potential due in part to their capacity to block carcinogenDNA adduct formation, modulate activi-ties of carcinogen-metabolizing enzymes, and suppress ROS in these noncancerous human breast cells.
-
Steven J.T. Jackson,Keith W. Singletary,Laura L. Murphy,Richard C. Venema,Andrew J. Young 한국식품영양과학회 2016 Journal of medicinal food Vol.19 No.1
Phytonutrients have rapidly emerged as natural food chemicals possessing multifaceted biological actions thatmay support beneficial health outcomes. Among the vast array of phytonutrients currently being studied, sulforaphane, curcumin, quercetin, and resveratrol have been frequently reported to stimulate the expression of endogenous detoxification enzymes and may thereby facilitate the neutralization of otherwise harmful environmental agents. Some of these same phytonutrients, however, have also been implicated in disrupting normal cell proliferation and hence may possess toxic properties in and of themselves. In this study, we characterize the respective minimum threshold concentrations of the aforementioned phytonutrients in Hepa1c1c7 cells that stimulate NAD(P)H:quinone oxidoreductase (NQO1), a key enzyme in the hepatic neutralization of menadione, other biological oxidants, and some environmental carcinogens. Moreover, our findings demonstrate that relatively low concentrations of either sulforaphane or curcumin significantly (P < .05) increase NQO1 protein expression and activity without triggering G2/M cell cycle arrest or mitotic catastrophe. The minimal quercetin concentration inducing NQO1, however, was 100-fold higher than that which disrupted mitosis. Also, while resveratrol modestly stimulated NQO1, the minimally effective resveratrol concentration concomitantly induced evidence of cellular apoptosis. Taken together, these findings indicate that only particular phytonutrients are likely efficacious in upregulating NQO1 activity without also leading to hepatic cytotoxicity.
연관 검색어 추천
이 검색어로 많이 본 자료
활용도 높은 자료
