Sequence analysis and expression profiles of TaABI5, a pre-harvest sprouting resistance gene in wheat

Ke Zhou,Jian Yang,Zao-Xia Wang,Ji-Rui Wang 한국유전학회 2017 Genes & Genomics Vol.39 No.2

Pre-harvest sprouting (PHS) is the germination of grains in the spike under wet conditions before harvest, which causes a severe reduction in crop yield and flour quality. The phytohormone abscisic acid plays a key role in regulating seed dormancy, which inhibits pre-harvest spouting or vivipary for many important crops. TaABI5, belonging to a family of basic leucine zipper transcription factors could regulate a subset of late embryogenesis abundant genes during seed development, was isolated and characterized. Phylogenetic analysis revealed that TaABI5 homologous gene belonged to the ABI5 subgroup of the ABF/AREB/ABI5 subfamily. TaABI5 consist of four conserved domains and one bZip domain. The expression pattern of TaABI5 indicated that it was seed-specific and accumulated at late stages of seed development. Accumulation of TaABI5 in synthetic hexaploid wheat SHW-L1 (PHS resistance) was 8.93-fold higher than that in wheat cultivar Chuanmai 32 (PHS susceptible) at 25 days post anthesis. Four expression quantitative trait loci (eQTL) of TaABI5 on chromosome 2DS, 4DS, 6DS, and 7DL were characterized from SHW-L1/Chuanmai 32 derived recombinant inbred line population.

Energy factor of high-strength-steel frames with energy dissipation bays under repeated near-field earthquakes

Ke Ke,Michael C.H. Yam,Xu-Hong Zhou,Fuming Wang,Fei Xu 국제구조공학회 2021 Steel and Composite Structures, An International J Vol.40 No.3

This investigation contributes to quantification of the inelastic seismic demands for high strength steel moment resisting frames equipped with energy dissipation bays (HSSF-EDBs) subjected to seismic sequences composed of repeated near-field ground motions. The emphasis is placed on the energy factor demand. A statistical examination of a database with more than eighty million energy factor demands of single-degree-of-freedom (SDOF) oscillators representing HSSF-EDBs responding in different yielding stages is conducted. The research findings show that in the damage-control stage, the energy factor which quantifies the peak seismic demand of a HSSF-EDB structure is insensitive to the repeated near-field earthquake motions. In contrast, a remarkable elevation of the energy factor is observed when oscillators characterising HSSF-EDBs progress into the ultimate stage. In addition, an increasing post-yielding stiffness ratio of the nonlinear force-displacement response in the damage-control stage may produce a detrimental effect on HSSF-EDBs progressing into the ultimate stage under repeated near-field earthquakes due to the corresponding evident increase of seismic demands. A nonlinear regression model quantifying the mean energy factor demand of the system under repeated near-field earthquake motions is proposed to facilitate performance-based seismic design of HSSF-EDBs.

Intraperitoneal Incubation of Bladder Acellular Matrix Grafts Improves Bladder Smooth Muscle Regeneration via Neovascularization

Zhou Zhe,Zhang Ming,Xu Mingxi,Zhang Ke,Zhao Yang,Zhou Juan,Zhu Yingjian,Wang Zhong,Lu Mujun 한국생물공학회 2015 Biotechnology and Bioprocess Engineering Vol.20 No.3

The objective of this study was to investigate whether intraperitoneal incubation improves the regenerative capacity of bladder acellular matrix grafts (BAMGs) in a rat model of bladder augmentation. After 2 weeks of incubation in the peritoneum of male rats, BAMG flaps with vascular pedicles were harvested for autologous bladder augmentation. As the control, BAMGs were directly used for bladder augmentation without intraperitoneal incubation. Histological analyses of the incubated BAMGs demonstrated extensive cell growth and vasculature in homogeneous collagen bundles. The cells were positive for vimentin and negative for α-smooth muscle actin and pan-cytokeratin AE1/AE3. Cystography revealed smoother contours of the augmented bladders in the incubated group at 4 and 12 weeks postoperatively. However, the bladder capacity was not significantly different between the two groups. In both groups, the entire urothelium regenerated well without obvious differences. At both time points, compared with the control group, increased numbers of smooth muscle cells (SMCs) and blood vessels were found in the incubated group. At 12 weeks, the SMCs in the incubated group were more similar to those in the native smooth muscle fiber bundles of the bladder. Taken together, our results demonstrated that BAMGs preincubated in the peritoneum promote the regeneration of bladder smooth muscle via neovascularization in a rat bladder augmentation model.

Flavonoid Glycosides and Potential Antivirus Activity of Isolated Compounds from the Leaves of Eucalyptus citriodora

Zhou, Zhong-Liu,Yin, Wen-Qing,Zou, Xiao-Peng,Huang, Dan-Ying,Zhou, Cui-Liu,Li, Lian-Mei,Chen, Ke-Cheng,Guo, Zi-Ying,Lin, San-Qing 한국응용생명화학회 2014 Applied Biological Chemistry (Appl Biol Chem) Vol.57 No.6

The extraction and solvent partition of the leaves of Eucalyptus citriodora, and repeated column chromatography for n-BuOH fraction yielded a new flavonoid glycoside, citrioside C (1), along with three known flavonoid glycosides (2-4). The latter were identified with kaempferol-3-O-${\beta}$-$\small{D}$-glucopyranosyl (12)-${\alpha}$-$\small{L}$-rhamnoside (2), kaempferol-3-O-${\alpha}$-$\small{L}$-rhamnoside (3), and quercetin-3-O-${\alpha}$-$\small{L}$-rhamnoside (4). Their chemical structures were identified on the basis of spectroscopic data analyses including NMR, MS, UV, and IR. All constitutents were isolated for the first time from the leaves of Eucalyptus citriodora. The potential antivirus activity of all the isolated compounds was evaluated. Compound 4 showed potent antiviral activity against respiratory syncytial virus with 50% inhibition concentration ($IC_{50}$) value of $1.9{\mu}g/mL$ and selective index value of 9.8.

Establishing a Nomogram for Stage IA-IIB Cervical Cancer Patients after Complete Resection

Zhou, Hang,Li, Xiong,Zhang, Yuan,Jia, Yao,Hu, Ting,Yang, Ru,Huang, Ke-Cheng,Chen, Zhi-Lan,Wang, Shao-Shuai,Tang, Fang-Xu,Zhou, Jin,Chen, Yi-Le,Wu, Li,Han, Xiao-Bing,Lin, Zhong-Qiu,Lu, Xiao-Mei,Xing, H Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.9

Background: This study aimed to establish a nomogram by combining clinicopathologic factors with overall survival of stage IA-IIB cervical cancer patients after complete resection with pelvic lymphadenectomy. Materials and Methods: This nomogram was based on a retrospective study on 1,563 stage IA-IIB cervical cancer patients who underwent complete resection and lymphadenectomy from 2002 to 2008. The nomogram was constructed based on multivariate analysis using Cox proportional hazard regression. The accuracy and discriminative ability of the nomogram were measured by concordance index (C-index) and calibration curve. Results: Multivariate analysis identified lymph node metastasis (LNM), lymph-vascular space invasion (LVSI), stromal invasion, parametrial invasion, tumor diameter and histology as independent prognostic factors associated with cervical cancer survival. These factors were selected for construction of the nomogram. The C-index of the nomogram was 0.71 (95% CI, 0.65 to 0.77), and calibration of the nomogram showed good agreement between the 5-year predicted survival and the actual observation. Conclusions: We developed a nomogram predicting 5-year overall survival of surgically treated stage IA-IIB cervical cancer patients. More comprehensive information that is provided by this nomogram could provide further insight into personalized therapy selection.

A novel prismatic-shaped isolation platform with tunable negative stiffness and enhanced quasi-zero stiffness effect

Ke Ke,Jing Bian,Xu-Hong Zhou,Michael C.H. Yam,Yu-Hang Wang,Zi Gu,Miaojun Sun 국제구조공학회 2023 Smart Structures and Systems, An International Jou Vol.31 No.3

A passive prismatic-shaped isolation platform (PIP) is proposed to realize enhanced quasi-zero stiffness (QZS) effect. The design concept uses a horizontal spring to produce a tunable negative stiffness and installs oblique springs inside the cells of the prismatic structure to provide a tunable positive stiffness. Therefore, the QZS effect can be achieved by combining the negative stiffness and the positive stiffness. To this aim, firstly, the mathematical modeling and the static analysis are conducted to demonstrate this idea and provide the design basis. Further, with the parametric study and the optimal design of the PIP, the enhanced QZS effect is achieved with widened QZS range and stable property. Moreover, the dynamic analysis is conducted to investigate the vibration isolation performance of the proposed PIP. The analysis results show that the widened QZS property can be achieved with the optimal designed structural parameters, and the proposed PIP has an excellent vibration isolation performance in the ultra-low frequency due to the enlarged QZS range. Compared with the traditional QZS isolator, the PIP shows better performance with a broader isolation frequency range and stable property under the large excitation amplitude.

중국 무역보험제도 및 Sinosure 개선 방안

주령커(ZHOU KING-KE),장동한(CHANG DONG-HAN) 한국무역학회 2016 무역학자 전국대회 발표논문집 Vol.2016 No.6

무역거래에서 발생하는 리스크는 일반적으로 부보가능한 리스크가 아니다. 무역이 국민경제에서 차지하는 중요성을 고려하여 각국은 WTO의 허락 하에서 독자적인 무역보험제도를 운영하고 있다. 중국도 국영기업인 Sinosure를 중심으로 무역보험제도를 운영하고 있다. 중국은 세계 최대 무역대국 중 하나임에도 불구하고 무역보험의 역사가 상대적으로 짧아 여러 문제점들을 안고 있다. 본 연구는 중국의 무역보험제도 및 운영 주체인 Sinosure의 문제점을 분석하고, 한국의 무역보험제도를 벤치마킹해서 중국 무역보험제도의 개선방안을 제시한다. Risks in international transaction usually cannot be covered by private insurance companies. Since international trade is a important part of national economy, WTO allows member state’s governments to operate the trade insurance system for the promotion of international trade, especially export. In China, state-owned insurance company, named Sinosure, is operating trade insurance system. Although china is a trade giant in the world, its history of trade insurance is rather short and there exist a number of problems in the trade insurance system of China. This study examines the problems in China’s trade insurance system and Sinosure, and then proposes the way to improve it based on the experience of Korea’s trade insurance system and Ksure.

Molecular Analyses of the Metallothionein Gene Family in Rice (Oryza sativa L.)

( Gong Ke Zhou ),( Yu Feng Xu ),( Ji Li ),( Ling Yan Yang ),( Jin Yuan Liu ) 생화학분자생물학회 2006 BMB Reports Vol.39 No.5

Probability Weighting Effect in Vertex Cover of Networks Via Prospect-Theoretic Learning

Xu, Ke,Wu, Ke,Zhou, Rongpei 한국통신학회 2023 Journal of communications and networks Vol.25 No.2

Game-theoretic learning methods for the vertexcover problem have been investigated in this paper. In thetraditional game theory, the establishment of the game modelis based on the complete objectivity of the players, and theexisting game models describe the vertex cover problem mainlyalong this path. In contrast, this paper considers the impactof players’ subjectivity on decision-making results. First, wepresent a covering game model, where the utility function ofthe player is established under the prospect theory. Then, bypresenting a rounding function, the states of all vertices underNash equilibrium satisfies vertex cover state of a general network. After then, we present a fictitious play distributed algorithm,which can guarantee that the states of all vertices converge aNash equilibrium. Finally, the simulation results are presentedto assess the impact of players’ subjectivity on the overall coverresults of networks.

Versicolols A and B, two new prenylated isocoumarins from endophytic fungus Aspergillus versicolor and their cytotoxic activity

Min Zhou,Jie Lou,Yin-Ke Li,Yue-De Wang,Kun Zhou,Bing-Kun Ji,Wei Dong,Xue-Mei Gao,Gang Du,Qiu-Fen Hu 대한약학회 2017 Archives of Pharmacal Research Vol.40 No.1

Versicolols A and B (1 and 2), two rareprenylated isocoumarin derivatives, along with five knownisocoumarins (3–7) were isolated from the fermentationproducts of an endophytic fungus Aspergillus versicolor. Their structures were elucidated on the basis of extensivespectroscopic analysis, including 1D- and 2D-NMR techniques. Compounds 1 and 2 were evaluated for their cytotoxicityagainst five human tumor cell lines. The resultsshowed that compounds 1 exhibited weak cytotoxicityagainst A549 and MCF7 cells with IC50 values of 9.4 and8.8 lm, and compound 2 exhibited weak cytotoxicityagainst SHSY5Y and MCF7 cells with IC50 values of 8.2and 6.8 lm, respectively.