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Comparative AFLP Profiles among Strains of Korean Races of Xanthomonas oryzae pv. oryzae.
Kang,Mi-Hyung,Lee,Du-Ku,Noh,Tae-Hwan,Shim,Hyeong-Kwon,Na,Seung-Yong,Kim,Jae-Duk 한국자원식물학회 2004 Plant Resources Vol.7 No.1
We used an amplified fragment length polymorphism (AFLP) analysis, a novel PCR-based technique, to differentiate Xanthomonas oryzae pv. oryzae (Xoo) of Korean races. The 6 strains of Xoo K1, K2, K3 races were tested with 81 AFLP primer combinations to identify the best selective primers. The primer combinations were selected according to their reproducibility, number of polymorphic bands and polymorphism detected among Xoo strains. 18 strains of Xoo K1, K2 and K3 races were analyzed with the selected combinations of primer set. Some primer combinations (Eco R I +1 / Mse I+1) could differentiate Xoo of Korean races that were not distinguished by other fingerprinting analysis. Thus AFLP fingerprinting permitted very fine discrimination among different races.
이중혈류유발 심폐소생술이 심정지를 유발한 개의 단기 생존율에 미치는 영향
황성오,조준휘,강구현,김성환,문중범,이강현,이승환,윤정한,최경훈,홍은석 대한응급의학회 2000 대한응급의학회지 Vol.11 No.3
Background and Objectives: We previously reported that, compared with standard cardiopulmonary resuscitation(S-CPR), better hemodynamic effects could be achieved by simultaneous sterno-thoracic cardiopulmonary resuscitation(SST-CPR) in which we compressed the sternum and constricted the thorax circumferentially during the systolic period by using a device. This study was designed to assess whether SST-CPR, compared with S-CPR, improve the survival rate of dogs with cardiac arrest. Subjects and methods: Twenty-five mongrel dogs(19∼31㎏) were enrolled in this study. After four minutes of ventricular fibrillation induced by an AC current, animals were randomized to resuscitate with either S-CPR(n=13) or SST-CPR(n=12). Epinephrine(1 ㎎) was injected into the right atrium every three minutes after the beginning of CPR. Defibrillation was attempted after 6 minutes of CPR. Standard advanced cardiac life support was started if defibrillation was not successful. Results: SST-CPR resulted in significantly(p<0.001) higher systolic arterial pressure(91±47 vs 47±24 ㎜ Hg), diastolic pressure(43±24 vs 17±10 ㎜ Hg), coronary perfusion pressure(35±25 vs 13±9㎜ Hg), and end tidal CO2 tension(9±4 vs 3±2 ㎜ Hg). Two of 13 animals(15 %) resuscitated with S-CPR and six of 12 animals(50%) resuscitated with SST-CPR survived until 12 hours after cardiac arrest(p<0.05). Donclusion: SST-CPR, compared with S-CPR, improves the short-term survival rate in canine cardiac arrest.
Kang, Hyung-Ku,Han, Jin-Woo,Kang, Soo-Hee,Kim, Jong-Hwan,Kim, Oung-Hwan,Hwang, Jeoung-Yeon,Seo, Dae-Shik The Korean Institute of Electrical and Electronic 2005 Transactions on Electrical and Electronic Material Vol.6 No.6
We studied the control of pretilt angles for homeotropic aligned nematic liquid crystal (NLC) on SiOx thin film surface by $45^{\circ}$ evaporation method with electron beam system. The uniform vertical LC alignment on. the SiOx thin film surfaces with electron beam evaporation was achieved. It is considered that the LC alignment on SiOx thin film by $45^{\circ}$ electron beam evaporation is attributed to elastic interaction between LC molecules and micro-grooves at the SiOx thin film surface created by evaporation. The pretilt angles of about $3.5^{\circ}$ in aligned NLC on SiOx thin film surfaces by electron beam evaporation of $45^{\circ}$ were measured. Consequently, the high pretilt angles of the NLC on the SiOx thin film by $45^{\circ}$ oblique electron beam evaporation method can be achieved.
Effect of increasing levels of apparent metabolizable energy on laying hens in barn system
Hwan Ku Kang,Seong Bok Park,전진주,Hyun Soo Kim,박기태,Sang Ho Kim,Eui Chul Hong,Chan Ho Kim 아세아·태평양축산학회 2018 Animal Bioscience Vol.31 No.11
Objective: This experiment was to investigate the effect of increasing levels of apparent metabolizable energy (AMEn) on the laying performance, egg quality, blood parameters, blood biochemistry, intestinal morphology, and apparent total tract digestibility (ATTD) of energy and nutrients in diets fed to laying hens. Methods: A total of three-hundred twenty 33-week-old Hy-Line Brown laying hens (Gallus domesticus) were evenly assigned to four experimental diets of 2,750, 2,850, 2,950, and 3,050 kcal AMEn/kg in pens with floors covered with deep litter of rice hulls. There were four replicates of each treatment, each consisting of 20 birds in a pen. Results: AMEn intake was increased (linear, p<0.05) with inclusion level of AMEn in diets increased. Feed intake and feed conversion ratio were improved (linear, p<0.01), but hen-day egg production tended to be increased with an increasing level of AMEn in diets. During the experiment, leukocyte concentration and blood biochemistry (total cholesterol, triglyceride, glucose, total protein, calcium, asparate aminotransferase, and alanine transferase were not influenced by increasing level of AMEn in diets. Gross energy and ether extract were increased (linear, p<0.01) as the inclusion level of AMEn in diets increased. Conclusion: Laying hens fed high AMEn diet (i.e., 3,050 kcal/kg in the current experiment) tended to overconsume energy with a positive effect on feed intake, feed conversion ratio, nutrient digestibility, and intestinal morphology but not on egg production and egg mass.
( Hwan Ku Kang ),( Chan Ho Kim ) 한국동물자원과학회(구 한국축산학회) 2016 한국축산학회지 Vol.58 No.3
Background: The objective of this experiment was to investigate the effects of dietary supplementation of rice bran oil (RBO) on growth performance, blood parameter, and immune response in broiler chickens. Methods: A total of 240 1-d-old ROSS 308 male broilers were randomly allotted to 4 dietary treatments with six replicated pens consisting of ten chicks. The basal diet was formulated to be adequate in energy and nutrients. Three additional diets were prepared by adding 5, 10 or 20 g/kg of RBO to the basal diet. The experimental diets were fed on an ad libitum basis to the birds during 35 d. Results: Results indicated that increasing inclusion level of RBO in diets improved BW gain (linear and quadratic, P < 0.01), improve feed conversion ratio (linear, P < 0.05) of birds during 0 to 35 d. There was no effect of inclusion level of RBO in diets on feed intake of birds. There was no effect of inclusion level of RBO in diets on erythrocytes of birds. However, heterophil, lymphocyte, and monocytes increased (linear and quadratic, P < 0.01) with inclusion level of RBO in diets increased. Feeding the diets containing increasing amount of RBO to birds increased (linear, P < 0.01) the concentrations of total cholesterol. Increasing inclusion level of RBO in diets increased concentrations of IgG (linear, P < 0.01). There was no effect of inclusion level of RBO in diets on concentrations of IgM. Conclusions: These results suggest that dietary RBO may be used functional ingredient to improve growth performance, total cholesterol in serum, and immune response of birds.
산란계 사료 내 사과박 발효물 및 계피의 첨가 · 급여가 생산성 및 계란 품질에 미치는 영향
강환구(Hwan Ku Kang),서옥석(Ok Suk Seo),최희철(Hee Chul Choi),채현석(Hyun Seok Chae),나재천(Jae Cheon Na),유동조(Dong Jo Yu),강근호(Guen Ho Kang),방한태(Han Tae Bang),박성복(Sung Bok Park),김민지(Min Ji Kim),이지은(Ji Eun Lee),김동욱(Do 韓國家禽學會 2010 韓國家禽學會誌 Vol.37 No.1
본 시험은 산란계 사료 내 사과박 발효물의 첨가가 산란계 생산성, 계란 품질 및 장내 미생물 변화에 미치는 영향을 조사하기 위하여 57주령 Hy-Line을 200수를 공시하여 5처리, 4반복, 반복당 10수씩 배치하여 10주간 사양 시험을 실시하였다. 시험구는 무첨가구(Control, C)와 산란계 사료 내 생균제 0.1% 첨가구(T1), 사과박 발효물 1.0% 첨가구(T2), 계피 0.1%첨가구(T3) 및 계피를 첨가한 사과박 발효물 1.0% 첨가구(T4)로 처리구를 나누어 시험을 실시하였다. 시험 전기간 동안 총 산란율에서는 각 처리구간 유의적인 차이는 인정되지 않았으나 대조구와 비교하였을 때 생균제 및 발효 사과부산물 첨가구에서 6.1% 개선되는 결과를 나타내었다. 계피 0.1% 첨가구에서는 무첨가구 대비 4.5% 개선되는 결과를 나타내었다. 시험 기간 동안 계란 품질 조사 결과 전체 처리구에서 유의적인 차이는 없었으나, 신선도의 경우 무첨가구와 비교 시 발효 사과 부산물 처리구에서 1.2%의 개선 효과를 나타내어 안전 양계 산물 생산에 대한 가능성을 나타내었다. 시험 전 기간 동안 혈액 생화학 및 혈구에 대해 처리구간 유의적인 차이는 나타나지 않았다. 결과적으로 산란계 사료 내 발효 사과 부산물의 첨가 · 급여는 산란계에 있어 생산성에 대해 개선 효과를 가지며, 향후 곡물 수급의 불안정 및 양계 분야에 대체 자원의 필요성을 고려할 때 농산부산물을 활용한다는 점에서 충분한 가능성을 시사한 결과라 할 수 있다. This experiment was conducted to investigate the effects of dietary supplementation of fermented apple pomace (FAP) and cinnamon on laying performance, egg quality and blood characteristics in laying hens. A total of two hundred 57-wk-old Hy-Line laying hens were divided into five groups with 4 replicates of 10 birds each; control, 0.1% probiotics, 1.0% fermented apple pomace 1.0%, 0.1% cinnamon and 1.0% fermented apple pomace with 1.0% cinnamon. Chickens fed the diets containing 1.0% of fermented apple pomace showed higher egg production than the chickens fed the other diets. No synergic effect on laying performance was found when fermented apple pomace and cinnamon were added to the diets in laying hens. There was no significant major differences in egg quality and serum blood biochemical profiles. These results suggest the possibility that fermentation of apple pomace could be used as the alternative of antibiotic growth promoters.