관동맥질환에서 저밀도 지단백 입자 크기의 분포

임효영,김인숙,승기배,강동헌,전두수,장기육,박준철,이수영,홍순조,최규보 대한순환기학회 1998 Korean Circulation Journal Vol.28 No.8

입력성형기법을 이용한 변위증폭형 마이크로스테이지 잔류진동제어

배규현,송은혜,강중옥,홍성욱 한국공작기계학회 2009 한국공작기계학회 춘계학술대회논문집 Vol.2009 No.-

This paper presents a result of application of input shaping to a micro-stage with a displacement amplification mechanism. The stage considered in this study is subjected to severe residual vibration in its lever mechanism to amplify displacement. The system dynamics exhibit some nonlinear nature due to the hysteresis of the PZT actuator. Input shaping method is successfully applied to the system so as to eliminate residual vibration from the system. The experimental study also shows that staircase input is useful to avoid the performance degrade by the nonlinear characteristics.

대농갱이 암,수의 성장 차이

강언종,이배익,조미영,손상규,김광석,김이청,방인철 한국어류학회 2003 韓國魚類學會誌 Vol.15 No.4

대농갱이는 동자개과의 담수어로 고부가가치를 창출 할 수 있는 새로운 담수 양식 대상종으로 주목받고 있으나 현재까지 성장도 등 양식에 대한 기초정보가 밝혀지지 않았으며, 본 연구에서는 인공으로 생산한 자 ? 치어를 사육하면서 암 ? 수의 성장 차이를 비교 ? 조사하였다. 암수의 정확한 성장 차이를 구명하기 위해 본 실험은 자어 초기 조직검사에 의한 조사와 치어기부터 표지법에 의한 조사로 구분하여 실시하였다. 조직학적 판별에 의한 암수간 성장비교 시험 결과 부화 후 50일까지는 수컷과 암컷의 성장차이는 없는 것으로 나타났으나, 부화 80일 후에는 수컷(체장 72.0±6.7㎜, 체중 3.71±6.7g이 암컷(체장 64.3±6.7㎜, 체중 3.71±0.99g)보다 성장이 빠른 것으로 나타나 대농갱이는 부화 후 50일에서 80일 사이에 암 ? 수의 성장차이가 시작되는 것으로 사료되었다. 부화 80일 이후 조사는 표지법이 개체 성장에 미치는 영향을 조사한 후 본 실험에 착수하였으며 표지구는 대조구와 사이에 유으적인 성장의 차이를 보이지 않았다. 개체간 성장차이는 조직학적 판별 결과와 동일한 경향으로 수컷이 암컷보다 성장이 유의적으로 빨랐으며(P<0.05), 시험 종료 후 암컷과 수컷의 크기는 수컷이 평균 체장 17.57㎝, 체중 52.19g으로 암컷의 평균 체장 11.02㎝, 체중 16.26g보다 체장은 약 1.5배, 체중은 약 3배이상 큰 것으로 나타났다(p=0.05). 체중으로 본 성장곡선은 지수성장을 보였으며, 수컷은 Y=0.0024X^(1.738)(R^(2)=0.985), 암컷은 Y=0.0405X^(1.0316)(R^(2)=0.983)의 지수식에 밀접하게 수렵되었고 성장의 차이는 사육기간이 길어질수록 크게 나타났다. The Ussurien bullhead, Leiocassis ussuriensis, of the family Bagridae has recently been targeted for freshwater culture in Korea. In this paper we investigated sexual dimorphism in body size in rearing condition using specimens produced artificially. Tissue sections of gonads were made between 20 and 80 days after hatching to identify sex of the specimens investigated. A difference in body size between sexes was not observed in fry until they were 50 days old. Male fry 80 days old had attained 81.62f7.21 ㎜ total length, and females 72.84±8.62 ㎜ total length. After 80 days the difference in body size was analysed by tagging. Applying the tag by cutting fin tips had no affect on the growth of the fishes (P< 0.05). The growth in body size of males was significantly faster (P< 0.05) than in females, and males attained about 1.5 times more in standard length and about 3 times more in body weight than females at the end of 320 days. The growth curve of body weight was exponential and the body weight was expressed by Y = 0.0024X^(1.738) (R² = 0.985) and Y = 0.0405X^(1.0316)(R² = 0.983) in male and female, respectively, observed at monthly intervals.

표고 버섯 중의 Branched chain amino acid Aminotransferase 의 분리정제 및 그 성질에 관한 연구

배강규,민태진,이수용 동국대학교 자연과학연구소 1987 자연과학연구 논문집 Vol.7 No.-

표고버섯중의 branched chain amino acid aminotransferase〔BCAT(EC 2,6,1,42)〕를(NH₄)₂SO₄ 분별침전, DEAE-cellulose column chromatography 및 sephades G-150겔 여과로 정제하여 그 특성을 조사하였다. 이 효소의 최적 pH는 8.5, 최적 온도는 36℃였고, 20℃이하에서 30분동안 열에 안정하였다. 이효소의 분자량은 69,000dalton이었으며, 산 가수분해하여 아미노산을 분석한 결과 18종의 서로 다른 아미노산들로 구성되어 있었다. 이 효소는 가지달린 아니노산인 L-valine, L-leucine 및 L-isoleucine에 대해서 특이성을 보였으며 phenylamine, potassium cyanide, phenylhydrazine, N-ethylmaleimide 및 p-chloromercuribenzoate에의하여 효소활성이 억제되었다. 또 한, L-leucine, a-ketoglutarate 및pyridoxal-5-phosphate에대한 이 효소의 Km값은 각 각 2.40mM, 2.98mM 및 1.74uM이었다. Branched cnain amino acid aminotransferaase 〔BCAT (EC 2.6.1.42)〕was purified by ammonium sulfate saturation. DEAE-cellulose column chromatography and sephadex G-150 gel filtration in Lentinus edodes(Berk.) Sing. Optimum pH and temperature were found to be 8.5 and 36℃, respectively. Apparent molecular weights of this enzyme was estimated 69.000 dalton by HPLC and SDS-PAGE. It showed activity toward L-leucine, L-isoleucine and L-valine as a substrate. It was ingibited by hydroxylamine, potassium cyanide, phenylgydrazine. N-ehylmaleimide, p-chloromercuribenzoate (CMB). cupric acetate and mercuric chloride. The Km values of this enzyme were determined to be 2.40mM for leucine, 2.98mM for a-ketoglutarate and 1.74μM for pyridexal-5- phospate, respectively.

다리뇌 경색의 단독증상으로 발생한 핵간 눈근육 마비 1예

박강민,배종석,김상진,김응규 인제대학교 2008 仁濟醫學 Vol.29 No.-

Internuclear ophthalmoplegia (INO) is caused by a lesion involving the medial longitudinal fasciculus (MLF) that interconnects the ocular motor nuclei. A 65-year-old woman with diabetes for twenty years developed diplopia, nausea, and vomiting one day before the presentation to the emergency room. Examination revealed adduction impairment of the right eye, combined with dissociated abducting nystagmus in the left eye. Other findings of the neuro-logical examination were normal. T2-weighted brain magnetic resonance imaging showed a hyperintensity lesion in right medial pons where the MLF locates. INO may be an isolated manifestation of pontine infarction.

버섯중 철이온에 활성화된 광감응성 Mitochondrial ATPase에 관한 연구

민태진,이미애,배강규 동국대학교 자연과학연구소 1993 자연과학연구 논문집 Vol.13 No.-

표고버섯중 광감응성 mitochondrial F?-ATPase는 Fe?, Fe? 및 M? 이온에 의하여 각각 활성화 되었으며 5.0mM Fe? 이온에 의한 상대활성도는 대조구에 비하여 107% 증가되었다. Mg? 존재하에서 Fe? 및 Fe? 각 이온 농도효과는 모두 효소의 활성을 증가 시켰으나 0.1mM Mg?과 5.0mM Fe? 이온의 공존하에서 170%를 증가시켜 Mg? 이온에 의한 상승작용을 보였다. 0.1mM Mg?과 0.1mM Fe? 존재하에서 Fe? 이온 농도효과는 그 농도가 5.0mM일 때 168%의 활성도 증가를 보여 Fe? 이온 공존 효과는 없었다. 이 효소는 Mg? 및 Fe? 이온에 으하여 활성화되는 특성을 가지고 있으며 활성 금속이온 존재하에서 측정한 최적 pH 및 온도는 각 각 7.5 및 66℃였다. The effects of the iron on the light-induced mitochondrial F?-ATPase in Lentinus edodes was studied. This enzyme activity was stimulated by each of the ferric, ferrous and magnesium ion. Especially, the activity of the enzyme by 5.0mF ferric ion increased up to 107% in comparision with control group(100%). In the presence of magnesium ion, each of ferric and ferrous ion increased the activity of the enzyme, particulary, coexistence of 0.1mM magnesium and 5.0mM ferric ion increased the activity up to 270% with magnesium ion dependence. The activity of the enzyme was stimulated up to 268% by 5.0mM ferric ionin the presence of 0.1mM magnesium and 0.1mM ferrous ion. Therefore, the coexistence of ferrous ion did not affect the activity. From the above, we propose that the light-induced mitochondrial F?-ATPase in L. edodes is a Mg?·Fe? f?-ATPase. The optimal pH and temperature for the enzyme were 7.5 and 66℃, respectively.

석이로부터 분리한 페놀성 화합물의 phospholipase A_2 저해활성

김진우,송경식,유익동,장현욱,유승현,배강규,민태진 충남대학교 생물공학연구소 1997 생물공학연구지 Vol.5 No.-

호알카리성 진균 Cephalosporium sp. RYM-202가 생산하는 alkaline xylanase (CX-III)의 작용에 의해 xylan 기질로부터 생성되는 주요 가수분해 산물은 xylobiose와 중합도가 4 이상인 xylooligosaccharides이었다. 이 효소는 xylobiose에 대한 분해능을 가지고 있지 않지만 xylotriose로부터는 xylobiose를, xylotetraose로부터는 xylobiose와 xylotriose를 주산물로 형성하였다. 이러한 결과들은 CX-III가 transglycosidase 활성을 소유하는 전형적인 endo-type xylanase임을 보여준다. N-bromosuccinimide에 의한 CX-III의 화학적 변화 실험 결과 효소 1분자 당 2개의 tryptophan 잔기가 활성에 관여하는 것으로 나타났다. 그러나 iodoacetamide 및 diethylpyrocarbonate에 의한 효소활성의 저해효과는 나타나지 않음으로써 이 효소의 활성부위에 cysteine과 histidine 잔기가 필수적이지 않음이 확인되었다. The hydrolysis products formed from birchwood xylan by the action of an alkaline xylanase (CX-III) from alkalophilic Cephalosporium sp. RYM-202 were xylobiose and xylooligosaccharides polymerized with more than 4 sugar molecules. This enzyme was not active on xylobiose but readily attacked xylotriose accumulating xylobiose as a major product. The predominant end-products from xylotetraose by CX-III were xylobiose and xylotriose. These results indicate that the enzyme is typically endo-type xylanase possessing transglycosidase activity. Chemaical modification of CX-III with N-bromosuccinimide revealed that two tryptophan residues per molecule of CX-III were essential for its catalytic activity on xylan. On the other hand, iodoacetamide and diethylpyrocarbonate did not influence the activity of the enzyme, suggesting that cysteine and histidine residues are not involved in the active site of this alkaline xylanase.

Metabolic Drug-Drug Interaction Potential of Macrolactin A and 7-<i>O</i>-Succinyl Macrolactin A Assessed by Evaluating Cytochrome P450 Inhibition and Induction and UDP-Glucuronosyltransferase Inhibition <i>In Vitro</i>

Bae, Soo Hyeon,Kwon, Min Jo,Park, Jung Bae,Kim, Doyun,Kim, Dong-Hee,Kang, Jae-Seon,Kim, Chun-Gyu,Oh, Euichaul,Bae, Soo Kyung American Society for Microbiology 2014 Antimicrobial Agents and Chemotherapy Vol.58 No.9

<P>Macrolactin A (MA) and 7-<I>O</I>-succinyl macrolactin A (SMA), polyene macrolides containing a 24-membered lactone ring, show antibiotic effects superior to those of teicoplanin against vancomycin-resistant enterococci and methicillin-resistant <I>Staphylococcus aureus</I>. MA and SMA are currently being evaluated as antitumor agents in preclinical studies in Korea. We evaluated the potential of MA and SMA for the inhibition or induction of human liver cytochrome P450 (CYP) enzymes and UDP-glucuronosyltransferases (UGTs) <I>in vitro</I> to assess their safety as new molecular entities. We demonstrated that MA and SMA are potent competitive inhibitors of CYP2C9, with <I>K<SUB>i</SUB></I> values of 4.06 μM and 10.6 μM, respectively. MA and SMA also weakly inhibited UGT1A1 activity, with <I>K<SUB>i</SUB></I> values of 40.1 μM and 65.3 μM, respectively. However, these macrolactins showed no time-dependent inactivation of the nine CYPs studied. In addition, MA and SMA did not induce CYP1A2, CYP2B6, or CYP3A4/5. On the basis of an <I>in vitro-in vivo</I> extrapolation, our data strongly suggested that MA and SMA are unlikely to cause clinically significant drug-drug interactions mediated via inhibition or induction of most of the CYPs involved in drug metabolism <I>in vivo</I>, except for the inhibition of CYP2C9 by MA. Similarly, MA and SMA are unlikely to inhibit the activity of UGT1A1, UGT1A4, UGT1A6, UGT1A9, and UGT2B7 enzymes <I>in vivo</I>. Although further investigations will be required to clarify the <I>in vivo</I> interactions of MA with CYP2C9-targeted drugs, our findings offer a clearer understanding and prediction of drug-drug interactions for the safe use of MA and SMA in clinical practice.</P>

Effect of anodic oxidation and precalcification treatments on Ti-6Al-4V alloy orthodontic miniscrew

Kang-Gyu Lee,Je-Hyeok Park,Jae Yoen Kang,Jin Jun,Cheol Soo Park,Byoung Gu Im,Tae-Sung Bae 대한치과재료학회 2019 대한치과기재학회 학술대회 Vol.2019 No.11

Concurrent chemoradiotherapy for elderly patients with stage III non-small cell lung cancer

Kang, Ki Mun,Jeong, Bae Kwon,Ha, In Bong,Chai, Gyu Young,Lee, Gyeong Won,Kim, Hoon Gu,Kang, Jung Hoon,Lee, Won Seob,Kang, Myoung Hee The Korean Society for Radiation Oncology 2012 Radiation Oncology Journal Vol.30 No.3

Purpose: Combined chemoradiotherapy is standard management for locally advanced non-small cell lung cancer (LA-NSCLC), but standard treatment for elderly patients with LA-NSCLC has not been confirmed yet. We evaluated the feasibility and efficacy of concurrent chemoradiotherapy (CCRT) for elderly patients with LA-NSCLC. Materials and Methods: Among patients older than 65 years with LA-NSCLC, 36 patients, who underwent CCRT were retrospectively analyzed. Chemotherapy was administered 3-5 times with 4 weeks interval during radiotherapy. Thoracic radiotherapy was delivered to the primary mass and regional lymph nodes. Total dose of 54-59.4 Gy (median, 59.4 Gy) in daily 1.8 Gy fractions and 5 fractions per week. Results: Regarding the response to treatment, complete response, partial response, and no response were shown in 16.7%, 66.7%, and 13.9%, respectively. The 1- and 2-year overall survival (OS) rates were 58.2% and 31.2%, respectively, and the median survival was 15 months. The 1- and 2-year progression-free survivals (PFS) were 41.2% and 19.5%, respectively, and the median PFS was 10 months. Regarding to the toxicity developed after CCRT, pneumonitis and esophagitis with grade 3 or higher were observed in 13.9% (5 patients) and 11.1% (4 patients), respectively. Treatment-related death was not observed. Conclusion: The treatment-related toxicity as esophagitis and pneumonitis were noticeably lower when was compared with the previously reported results, and the survival rate was higher than radiotherapy alone. The results indicate that CCRT is an effective in terms of survival and treatment related toxicity for elderly patients over 65 years old with LA-NSCLC.