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Park, Junghee,Han, Ji-Hye,Myung, Seung-Hyun,Seo, Young-Woo,Kim, Tae-Hyoung Elsevier 2018 Biochemical and biophysical research communication Vol.495 No.2
<P><B>Abstract</B></P> <P>The mitochondrial targeting domain (MTD) of Noxa has necrosis-inducing activity when conjugated with cell-penetrating peptide (CPP). In this study, we report another MTD-like motif, B1MLM, found in BNIP1, a pro-apoptotic BH3-only protein found in the endoplasmic reticulum membrane. The B1MLM peptide, conjugated with CPP, induced necrosis in a way similar to that of R8:MTD. R8:B1MLM caused an intracellular calcium spike, mitochondrial reactive oxygen species generation, and mitochondrial fragmentation. The cytosolic calcium spike was likely due to the opening of the mitochondrial permeability transition pore.</P>
Park, Junghee,Han, Ji-Hye,Myung, Seung-Hyun,Kang, Hyuno,Cho, Ju-Yeon,Kim, Tae-Hyoung Academic Press 2019 Biochemical and biophysical research communication Vol. No.
<P><B>Abstract</B></P> <P>Noxa is a weak apoptosis activator consisting of a BH3 domain and a mitochondrial-targeting domain (MTD). BH3 binds Mcl-1 and Bcl2A1 and inactivates their anti-apoptotic activities, while MTD delivers BH3 to mitochondria. Previously we revealed that MTD may also function as an inducer of necrosis via conjugation with octa-arginine, which induces cytosolic Ca<SUP>2+</SUP> influx from mitochondria. However, the mechanism(s) underlying this process has not been elucidated yet. Here, we show that calcium influx induced by an MTD peptide fused with octa-arginine residue (R8:MTD) originates not only from mitochondria but also from the extracellular space. However, calcium spikes were not sufficient for necrosis. R8:MTD induced mitochondrial permeability transition pore opening, fragmentation, and swelling. These mitochondrial events induced by MTD appeared to be necessary for necrosis induction, since DIDS, a VDAC inhibitor, inhibited the mitochondrial swelling and cell death induced by MTD. We show that R8:MTD disrupted endoplasmic reticulum (ER) structures but not peroxisomes or Golgi, indicating that R8:MTD causes necrosis by inducing ER events as well.</P> <P><B>Highlights</B></P> <P> <UL> <LI> MTD peptide fused with 8-arginine (R8:MTD) induced necrotic cell death. </LI> <LI> R8:MTD induced Ca<SUP>2+</SUP> influx from mitochondria and extracellular space. </LI> <LI> MTD interacted with VDAC, and induced mitochondrial catastrophe. </LI> <LI> VDAC inhibitor DIDS inhibited cell death and mitochondrial disruption. </LI> <LI> R8:MTD disrupted ER where VDAC was expressed. </LI> </UL> </P>
( Junghee Park ),( Ji-hye Han ),( Seung-hyun Myung ),( Hea-jong Chung ),( Jae-il Park ),( Ju-yeon Cho ),( Tae-hyoung Kim ) 한국미생물생명공학회(구 한국산업미생물학회) 2021 Journal of microbiology and biotechnology Vol.31 No.6
The mitochondrial targeting domain (MTD) of Noxa contributes to its mitochondrial localization and to apoptosis induction. As a peptide, MTD fused with octa-arginine (R8), a CPP, induces necrosis related to intracellular calcium influx and destruction of mitochondria and endoplasmic reticulum. We searched for homologs of MTD, and compared their cell killing capability when fused with R8. Three of the seven peptides triggered cell death with similar mechanisms. The comparative analysis of peptide sequences showed that four amino acid sites of MTD are critical in regulating necrosis, suggesting the potential to generate artificial, adjustable cytotoxic peptides, which could be effective medicines for many diseases. Thus, homologs functionality could hint to the functions of their belonging proteins.
Park, Yujin,Yun, Junghee,Park, Dongchul,Kim, Sangwoo,Kim, Suhwan IEEE 2018 IEEE transactions on very large scale integration Vol.26 No.1
<P>The size and power dissipation of an infrared imaging system can be reduced by the use of uncooled microbolometers; but the nonuniformity of the microbolometer makes such imaging systems heavily reliant on complicated calibration techniques, incurring an overhead which is particularly significant in low-cost, compact devices. We therefore propose a shutter-based successive-approximation calibration loop, which avoids the need to implement correction tables in software on an external processor. Prototype imager, consisting of an <TEX>$80 \times 82$</TEX> pixel infrared focal-plane array and readout circuitry, has been implemented, and the experimental results confirm that our on-chip autocalibration approach compensates effectively for fixed pattern noise caused by the nonuniformity of the microbolometers.</P>
Literature Review about Virtual Reality-Based Education for Healthcare Students
Junghee Park,Byoung-Gil Yoon,Yong-Seok Kim,Hyunjung Lee 국제문화기술진흥원 2022 International Journal of Advanced Culture Technolo Vol.10 No.4
The purpose of this study was to analyzed the current status of the of the Virtual Reality-based education for healthcare students in Korea. Five databases (KoreaMed, KMbase, NDSL, KISS, KiSTi) were searched prior to July 2022. A total 10 articles met the inclusion criteria. 5 articles developed VR-based education contents and the other 5 articles were using vSim already developed as a VR education program and evaluated the effectiveness. As a result of this literature review, VR-based education program was found to be effective in improving the healthcare students’ competency. VR-based education program could be considered to improve the healthcare students’ clinical practice ability.