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( Jennifer Jooyoun Kim ),( Young Kyung Kwon ),( Ji Hyung Kim ),( Soo Jin Heo ),( Young Deuk Lee ),( Su Jin Lee ),( Won Bo Shim ),( Won Kyo Jung ),( Jung Ho Hyun ),( Kae Kyoung Kwon ),( Do Hyung Kang ) 한국미생물 · 생명공학회 2014 Journal of microbiology and biotechnology Vol.24 No.11
Cellulase and xylanase are main hydrolysis enzymes for the degradation of cellulosic and hemicellulosic biomass, respectively. In this study, our aim was to develop and test the efficacy of a rapid, high-throughput method to screen hydrolytic-enzyme-producing microbes. To accomplish this, we modified the 3,5-dinitrosalicylic acid (DNS) method for microwell plate-based screening. Targeted microbial samples were initially cultured on agar plates with both cellulose and xylan as substrates. Then, isolated colonies were subcultured in broth media containing yeast extract and either cellulose or xylan. The supernatants of the culture broth were tested with our modified DNS screening method in a 96-microwell plate, with a 200 μl total reaction volume. In addition, the stability and reliability of glucose and xylose standards, which were used to determine the enzymatic activity, were studied at 100ºC for different time intervals in a dry oven. It was concluded that the minimum incubation time required for stable color development of the standard solution is 20 min. With this technique, we successfully screened 21 and 31 cellulase- and xylanase-producing strains, respectively, in a single experimental trial. Among the identified strains, 19 showed both cellulose and xylan hydrolyzing activities. These microbes can be applied to bioethanol production from cellulosic and hemicellulosic biomass.
( Jennifer Ann Clarke ),( Hyun-chul Jung ),( Hyo-jung Kang ),( Myong-won Seo ),( Jong-kook Song ) 국제태권도학회 2016 Journal of the International Association for Taekw Vol.3 No.2
This study examined the long-term effects of Taekwondo training on bone mineral density (BMD), body composition, and physical fitness in middle-aged men. Thirty men (43 ± 2 yr) were divided into three groups based on Taekwondo experience and current training volume. The currently practicing Taekwondo group (TKDC: n = 10, training career: 34.1 yr) practiced Taekwondo ≥ 2 times per week. The former Taekwondo group (TKDS: n = 10, training career: 19.2 yr) trained during childhood and adolescence, but ceased training before age 30. The control group (CON: n = 10) received no regular physical training during youth. The results showed that both Taekwondo groups had a significantly higher BMD, (p < 0.004) and Z-scores (p < 0.008) for femoral neck and L4 BMD (p < 0.023). For physical fitness, both groups showed significantly better results for 50m shuttle run (p < 0.001) and sit-ups (p < 0.0004) than the control group. No significant difference was found in body composition among the groups. In conclusion, TKDC and TKDS may have had site-specific benefits for BMD. As there was no significant difference in these variables between the two Taekwondo groups, the benefits were most likely gained during childhood and adolescence, and further training did not appear to increase these gains. More research is needed with a larger sample size to confirm these findings.
The New Woman and New-Style Weddings in Colonial Korea
Jennifer Jung-Kim 한국학중앙연구원 한국학중앙연구원 2008 THE REVIEW OF KOREAN STUDIES Vol.11 No.4
Wedding ceremonies of the early modern period became newly invented wedding “traditions” in a nascent form of transculturalism as Christian, Buddhist, and other “modern” wedding ceremonies, collectively called sinsik gyeolhon (new-style weddings), first emerged in the 1890s and became commonplace by the early 1920s. Some of the most noticeable changes in wedding ceremonies were the ways in which they became hybridized invented traditions, selectively choosing aspects of both “old” and “new” weddings. Weddings also became commercialized affairs, an emblem of urban middle- and upperclass culture in colonial Korea. When we examine all these aspects, we can see that weddings reflected not only social trends, but also the anxieties of the times. In addition to more recent works on weddings, I rely on primary sources such as newspaper and magazine articles as well as photographs from the colonial period to see how Koreans negotiated transcultural influences to produce weddings as invented traditions and how wedding practices became commercialized. By looking at hybridity and commercialization as closely related processes, this paper examines ways in which wedding ceremonies transformed in form and symbolism from the late nineteenth century through the colonial period. Wedding ceremonies of the early modern period became newly invented wedding “traditions” in a nascent form of transculturalism as Christian, Buddhist, and other “modern” wedding ceremonies, collectively called sinsik gyeolhon (new-style weddings), first emerged in the 1890s and became commonplace by the early 1920s. Some of the most noticeable changes in wedding ceremonies were the ways in which they became hybridized invented traditions, selectively choosing aspects of both “old” and “new” weddings. Weddings also became commercialized affairs, an emblem of urban middle- and upperclass culture in colonial Korea. When we examine all these aspects, we can see that weddings reflected not only social trends, but also the anxieties of the times. In addition to more recent works on weddings, I rely on primary sources such as newspaper and magazine articles as well as photographs from the colonial period to see how Koreans negotiated transcultural influences to produce weddings as invented traditions and how wedding practices became commercialized. By looking at hybridity and commercialization as closely related processes, this paper examines ways in which wedding ceremonies transformed in form and symbolism from the late nineteenth century through the colonial period.
세포 용해를 위한 in-situ 합성 및 배열 나노와이어 미세유체 소자
김정(Jung Kim),홍정우(Jung Woo Hong),Zhiyong Li,신현정(Jennifer H. Shin),박인규(Inkyu Park) 대한기계학회 2011 대한기계학회 춘추학술대회 Vol.2011 No.10
Cell lysis is an essential step for intracellular analysis. Among many chemical and physical methods, mechanical means of cell lysis have recently been in spotlight due to its efficiency. In this study, we introduce a novel microfluidic platform for cell lysis consisting of an in-situ patterned synthesized array of ZnO nanowires. Nanowires were grown in the microchannel by seed-mediated hydrothermal reaction under low-temperature, which allows extremely convenient route for the patterned synthesis and direct integration of ZnO nanowires within a microfluidic channel. Our preliminary results in testing nanowires for cell lysis have revealed a good lysis performance, showing instantaneous staining of intracellular structures due to cell rupture and high protein levels on the resultant cell lysate. We expect that this novel method will be utilized in easy and cost-effective fabrication of microfluidic cell analysis device with less denaturing of intracellular molecules.
Lee, Jennifer,Lee, Ji-Yeon,Lee, Jae-Ho,Jung, Seung-Min,Suh, Young Sun,Koh, Jung-Hee,Kwok, Seung-Ki,Ju, Ji Hyeon,Park, Kyung-Su,Park, Sung-Hwan BioMed Central 2015 ARTHRITIS RESEARCH AND THERAPY Vol.17 No.-
<P><B>Introduction</B></P><P>Gout is a chronic inflammatory disease the development of which is associated with obesity-induced metabolic abnormalities. However, a substantial number of non-obese patients (body mass index [BMI] <25 kg/m<SUP>2</SUP>) also develop gout in Korea. It was suggested that accumulation of visceral fat rather than subcutaneous fat is associated with metabolic abnormalities and hyperuricemia in patients with gout; therefore, we hypothesized that visceral fat accumulation was increased in non-obese gout patients.</P><P><B>Methods</B></P><P>One hundred and three male patients with primary gout and 204 age-matched healthy controls who attended a health check-up examination were recruited after the review of medical charts. The visceral fat area (VFA) was measured using the bioelectrical impedance analysis (BIA) method, and a VFA >100 cm<SUP>2</SUP> was defined as visceral fat obesity (VFO). The frequency of VFO was compared in patients and control groups. The frequencies of metabolic syndrome and related parameters were also investigated.</P><P><B>Results</B></P><P>BMI, waist circumference, total fat mass, serum triglycerides, and serum glucose levels were significantly greater in patients compared with controls. VFA and the prevalence of VFO was increased in gout patients compared with controls. There were positive correlations between VFA and serum triglyceride levels and serum glucose levels. Multivariate regression analysis revealed that VFO is an independent risk factor for gout (odds ratio 2.488, 95% confidence interval 1.041–4.435). In non-obese subgroup analyses (gout patients, <I>n</I> = 38; healthy controls, <I>n</I> = 150), VFA (98.7 ± 19.3 vs. 91.0 ± 16.7, <I>P</I> = 0.016) and the frequency of VFO (47.4 vs. 27.3%, <I>P</I> = 0.017) remained significantly higher in gout patients. There was no difference in either BMI or total fat mass between patients and controls in the non-obese subgroup. The prevalence of metabolic syndrome in patients with gout was 31.7% (33/104), compared with 13.2% (5/38) in the non-obese subgroup according to modified ATP III criteria.</P><P><B>Conclusion</B></P><P>VFO, measured using BIA, is observed more frequently in patients with primary gout compared with healthy controls, even in non-obese individuals. Therefore, VFO might more properly represent metabolic derangements in patients with gout than general obesity.</P>