Production of Mycelium and Monacolin K from Monascus sp. on Rice Solid Culture

Hyuck-Jun Jung(정혁준),Chi Duck Park(박치덕),Sung Min Park(박성민),Hyun Soo Kim(김현수),Tae Shick Yu(유대식) 한국생물공학회 2004 한국생물공학회 학술대회 Vol.2004 No.4

NTG에 의한 Amylase활성이 높은 누룩사상균의 변이주의 분리

정혁준(Hyuck-Jun Jung),김영숙(Young-Sook Kim),유대식(Tae-Shick Yu) 한국식품영양과학회 2000 한국식품영양과학회지 Vol.29 No.6

한국 전통누룩으로부터 분리ㆍ동정된 Asp. coreanus NR 15-1, Asp. oryzae NR 15-3, 그리고 Asp. oryzae NR 2-5로부터 amylase활성이 높고 세포융합에 사용될 변이주를 분리하고 그의 특성을 검토했다. Aspergillus속 사상균을 변이처리하기 위한 변이원으로는 NTG를 사용하였으며 Asp. coreanus NR 15-1로부터 총 15종의 변이주중 8종의 영양요구변이주, Asp. oryzae NR 15-3으로부터 총 5종중 3종의 영양요구변이주를, Asp. oryzae NR 2-5로부터 총 6종중 2종의 영양요구변이주를 분리하였다. 이들 영양요구변이주는 최소배지에서는 생육하지 않았으며 특정 아미노 산과 핵산염기 등을 배지에 첨가했을 경우에만 생육하였다. 분리된 변이주의 당화력, 액화력 및 산 생성능 등을 검토한 결과, 산 생성능이 우수한 균주인 Asp. coreanus NR 15-1로부터 glucoamylase 활성이 높은 X11(Arg.-) 변이주와 Asp. oryzae NR 2-5로부터 α-amylase 활성이 우수한 영양요구변이주인 Z6(Ade.-) 변이주를 세포융합에 사용이 가능한 변이주라 사료되었다. 앞으로 이들의 변이주를 대상으로 세포융합을 통해 우수한 사상균주를 획득할 수 있는 기초적 자료가 됨으로서 전통누룩의 과학화에 기여할 뿐만 아니라 외국 주류와 경쟁력이 있고 과학화된 전통주류의 개발이 가능하리라 사료된다. Aspergillus coreanus NR 15-1, Asp. oryzae NR 15-3 and Asp. oryzae NR 2-5 isolated from traditional Korean nuruk were screened as parental strains producing starch hydrolyzing enzymes. They were mutagenized by N-methyl-N'-nitro-N-nitrosoguanidine (NTG) and mutants were isolated for analysis of various amylase activities and the ability of acid production. Among them, the mutants harboring high saccharogenic activity, dextrinogenic activity, and the ability of acid production were selected. Fifteen, six, and five strains of mutants were isolated from Asp. coreanus NR 15-1, Asp. oryzae NR 2-5, and Asp. oryzae NR 15-3, respectively followed by NTG mutagenesis. Among these mutants, thirteen strains were identified as auxotrophic mutants. X11 (Arg.-) mutant from Asp. coreanus NR 15-1 showed high glucoamylase activity and total acid productivity. Z6 (Ade.-) mutant from Asp. oryzae NR 2-5 showed the highest α-amylase activity, therefore X11 and Z6 mutant were selected.

생활체육 검도수련이 정신건강에 미치는 영향

정혁준(Hyuck-Jun Jung),김종언(Jong-Eon Kim) 대한검도학회 2014 대한검도학회지 Vol.25 No.2

본 연구는 생활체육 검도수련이 정신건강에 미치는 영향을 조사하고 분석 하는 것을 목적으로 경기도 수원, 분당 지역 검도장의 수련자와 비수련자 300명을 대상으로 설문지를 배포한 후 자기평가 기입법(Self-administration method)으로 작성하게 하였다. 본 연구에서 사용한 자료분석 기법은 빈도분석(Frequency Analysis), 독립표본 T-test(Independent Group t-test)와 일원변량분석(one way-anova), 이원변량분석(Two way-anova)이었다. 위의 연구방법을 통하여 자료를 분석하고 다음과 같은 결론을 도출하였다. 첫째, 생활체육 검도수련자와 비수련자의 정신건강의 차이에서는 수련자보다 비수련자의 정신건강이 높게 나왔고 통계적으로 유의미한 차이를 보였다. 둘째, “수련 횟수에 따라 수련자의 정신건강은 차이가 있을 것이다.”는 우울증상에서만 수련 횟수 1회인 경우가 주 2회~3회인 경우보다 높게 나왔지만 전체적으로 수련 횟수가 증가함에 따라 우울, 불안, 정신증, 신체화, 전체 부정적 정신건강도 함께 증가한다. 셋째, 수련자의 단(급)수에 따른 정신건강의 차이는 없는 것을 알 수 있고, 통계적으로도 유의미한 차이가 없었다. 넷째, 성별에 따라 수련자와 비수련자의 정신건강 차이에서 수련 여부에 의한 정신건강의 차이는 있지만. 성별에 따른 정신건강의 차이는 없다. 다섯째, 연령에 따라 수련자와 비수련자의 정신건강 차이에서 수련 여부에 의한 정신건강의 차이는 있지만 성별에 따라 정신건강의 차이는 없다. 이상 결론을 종합해 보면 검도수련자와 비수련자 정신건강 차이는 비수련자가 더 높게 나와 검도수련이 정신건강에 미치는 영향이 있다고 알 수 있고 검도수련자만을 대상으로 보면 성별, 연령, 수련경력, 단(급)수에 따른 정신건강의 차이는 없고 수련 횟수에 따라서 정신건강의 차이도 높아짐을 알 수 있다. This paper is about Effects of Kumdo training on mental health. A total of 300 questionnaires were sent out for the survey. 300 people of Kumdo practitioners and non practitioners in regions Gyeonggi-do Suwon, Bundang are asked to fill out a questionnaire in the way of Self-administration method. The returned 278 questionnaires are analyzed by using Cronbach"s Alpha Coefficient. Reliability was measured over .6 on all the items which means there are very high internal consistency between each question. Among the returned questionnaires, 22 questionnaires are excepted because of unclear and uncertain attitude in answering. Data analysis techniques used in this study are Frequency Analysis, T-test(Independent Group t-test), One way-anova and Two way-anova. The results of data analysis through those research methods and conclusions are as follows. First, non practitioners are in high levels of mental health than practitioners. Second, Third, although depression symptoms are decreased by increasing the number of training, anxiety, psychoticism, somatization, and all the negative mental health are increased by increasing the number of training. Fourth, by practitioners" rating, there is no difference in levels of mental health. Fifth, there is difference in levels of mental health not by practitioners" gender, but by whether practitioners practice Kumdo or not. Taken together, Kumdo training affects on practitioners" mental health. There are significant differences in levels of mental health not because of gender, age, practitioners" rating but because of how often practicing Kumdo. In conclusion, Kumdo training has positive effect on practitioner"s mental health, if practitioners could see the number of proper times in training Kumdo for themselves.

유전자조작 , 균주분리 ( Aspergillus coreanus NR 15-1 과 Aspergillus oryzae NR 2-5 의 원형질체 형성의 최적조건

정혁준(Hyuck Jun Jung),유대식(Tae Shick Yu) 한국미생물생명공학회 2001 Korean Journal of Applied Microbiology and Biotech Vol.29 No.1

N/A N/A

Kohonen 학습의 입력에 잡음 주입의 효과

정혁준(Hyuck-jun Jung),송근배(Geunbae Song),이행세(Haingsei Lee) 대한전자공학회 2001 대한전자공학회 학술대회 Vol.2001 No.6

Clinicopathologic risk factors for the local recurrence of phyllodes tumors of the breast.

Jang, Jae Hyuck,Choi, Min-Young,Lee, Se Kyung,Kim, Sangmin,Kim, Jiyoung,Lee, Jeonghui,Jung, Seung Pil,Choe, Jun-Ho,Kim, Jung-Han,Kim, Jee Soo,Cho, Eun Yoon,Lee, Jeong Eon,Nam, Seok Jin,Yang, Jung-Hyun Raven Press 2012 Annals of surgical oncology Vol.19 No.8

<P>Phyllodes tumors (PTs) are rare breast tumors that usually occur in middle-aged women. Here we discuss our recent experiences in the diagnosis, surgical management, and clinical follow-up of this disease.</P>

Structural Insights into the Quaternary Catalytic Mechanism of Hexameric Human Ouinolinate Phosphoribosyltransferase, a Key Enzyme in de novo NAD Biosynthesis

( Hyung-seop Youn ),( Tae Gyun Kim ),( Mun-kyoung Kim ),( Gil Bu Kang ),( Jung Youn Kang ),( Jung-gyu Lee ),( Jun Yop An ),( Kyoung Ryoung Park ),( Youngjin Lee ),( Young Jun Im ),( Jun Hyuck Lee ),( 전남대학교 약품개발연구소 2016 약품개발연구지 Vol.25 No.-

Ouinolinate phosphoribosyltransferase (QPRT) catalyses the production of nicotinic acid Mononucleotide, a precursor of de novo biosynthesis of the ubiquitous coenzyme nicotinamide adenine dinucleotide. QPRT is also essential for maintaining the homeostasis of quinolinic acid in the brain, a possible neurptoxin causing various neurodegenerative diseases. Although QPRT has been extensively analysed, the molecular basis of the reaction catalyzed by human QPRT remains unclear. Here, we present the crystal structures of hexameric human QPRT in the apo form and its complexes With reactant or product. We found that the interaction between dimeric subunits was dramatically altered during the reaction process by conformational changes of two flexible loops in the active site at the dimer-dimer interface. In addition, the N-terminal short helix α1 was identified as a critical hexamer stabilizer. The structural features, size distribution, heat aggregation and ITC studies of the full-length enzyme and the enzyme lacking helix α1 stroungly suggest that human QPRT acts as a hexamer for cooperative reactant binding via three dimeric subunits and maintaining stability. Based on our comparison of human QPRT structures in the apo and complex forms, we propose a drug design strategy targeting malignant glioma.

S-131 Incidence of Hepatocellular carcinoma in korean screenee with chronic hepatitis B

( Jung Rock Moon ),( Jae-jun Shim ),( Tae-woong Choi ),( Chi Hyuck Oh ),( Soyung Park ),( Yu Jin Um ),( Byung-ho Kim ) 대한내과학회 2016 대한내과학회 추계학술대회 Vol.2016 No.1

Background: To optimize efficacy of National Liver Cancer Screening Program (NLCSP) for subjects with HBsAg positive, it is crucial to know the incidence of HCC and its predisposing factors in the program. Methods: From January 2010 to December 2014, all the HBsAg positive participants who received at least two or more abdominal ultrasonography under NLCSP were retrospectively enrolled in a single tertiary hospital. Annual incidence of HBV-related HCC was calculated and related clinical factors were investigated. Results: During 5 years, 541 subjects were enrolled. Liver cirrhosis was diagnosed in 212 (39.2%) by ultrasonography or upper endoscopy. Esophageal varices were found in 63 (14.8%). Total bilirubin, albumin, platelets, and aminotransferases were normal in most subjects. HBV DNA were less than 2,000 IU/mL in 356 subjects (79.6%). Mean follow-up time was 2.4 years and 16 new HCCs were diagnosed. Annual incidence of HBV-related HCCs were 980 per 100,000 patient year (1% per year). Subjects more than 60 years old (2.2% per year) had higher risk of HCC development than those under 60 years (0.6% per year, p<0.005). Presence of LC (2.2% per year) also showed higher risk of HCC than LC-free state (0.2% per year, p<0.0001). In cirrhotic patients over 60 years old, the incidence increased up to 3.8% per year.Conclusions: Despite of high rate of antiviral therapy, incidence of HBV-related HCC is not low in participant of NLCSP in Korea. Old age and presence of LC are associated with higher risk of HCC development.

Markov Blanket Feature Selection for Classification

Jung-Hye Lee(이정혜),Chi-Hyuck Jun(전치혁) 대한산업공학회 2013 대한산업공학회 추계학술대회논문집 Vol.2013 No.11

When the dimensionality is very large, exponential growth in the amount of the data is required for a classification task. Thus, reducing the dimensionality of the data is essential, and it can often bring the better performance. As one of dimension reduction techniques, Markov blanket can be considered as a filter method which is the minimal variable set to explain the target variable. We apply Markov blanket feature selection for categorical data, and then compare it with other feature selection methods using well-known classifiers. We successfully show the out performance of Markov blanket feature selection method. In addition, by implementing several Markov blanket induction algorithms also, we point out the performance of each Markov blanket algorithm depending on data.

Evaluation of Ceftriaxone Utilization at Multicenter Study

( Hyuck Lee ),( Dong Sik Jung ),( Joon Sup Yeom ),( Jun Seong Son ),( Sook In Jung ),( Yeon Sook Kim ),( Chun Kwan Kim ),( Hyun Ha Chang ),( Shin Woo Kim ),( Hyun Kyun Ki ),( Chi Sook Moon ),( Doo Rye 대한내과학회 2009 The Korean Journal of Internal Medicine Vol.24 No.4

Background/Aims: As bacterial resistance to antimicrobial agents has grown due to the increasing use of antimicrobial agents, we sought to evaluate the suitability of ceftriaxone usage (representative of third generation cephalosporins) at 10 university hospitals in Korea. Methods: We prospectively evaluated the appropriateness of antibiotic usage in 400 adult patients who received ceftriaxone between February 1, 2006 and June 30, 2006. Drug utilization evaluation (DUE) methods were based on standards set forth by the American Society of Hospital Pharmacists. The DUE criteria used in this study were modified to be more suitable in our hospital setting: justification of drug use, critical and process indications, complications, and outcome measures. Results: The average patient age was 64.4 years. The utilization of ceftriaxone was appropriate in 262 cases (65.5%) for the justification of use, while inappropriate use was observed in 138 cases (34.5%). Common reasons for inappropriate use of ceftriaxone included continued empiric use for presumed infections, prophylactic perioperative injection, and empiric therapy for fever. Most of the critical indications showed a high rate of suitability (66.5-98.5%). Complications occurred in 37 cases (9.3%). With respect to outcome measures, clinical responses were observed in 60.7% of cases, while only 15.7% of cases showed evidence of infection eradication via negative cultures. Conclusions: Appropriate use (65.5%) of ceftriaxone was higher than inappropriate use (34.5%) at university hospitals in Korea. Inappropriate utilization, however, including continued empiric use for presumed infections and prophylactic perioperative injection remained high. Intensification of educational programs and antibiotic control systems for ceftriaxone is needed to improve the suitability of antimicrobial use. (Korean J Intern Med 2009;24:374-380)