KBTBD7, a novel human BTB-kelch protein, activates transcriptional activities of SRE and AP-1

( Jun Jian Hu ),( Wu Zhou Yuan ),( Ming Tang ),( Yue Qun Wang ),( Xiong Wei Fan ),( Xiao Yang Mo ),( Yong Qing Li ),( Zao Chu Ying ),( Yong Qi Wan ),( Karen Ocorr ),( Rolf Bodmer ),( Yun Deng ),( Xiu 생화학분자생물학회 2010 BMB Reports Vol.43 No.1

Edge-thionic acid-functionalized graphene nanoplatelets as anode materials for high-rate lithium ion batteries

Fan, Qinghua,Noh, Hyuk-Jun,Wei, Zengxi,Zhang, Jiakui,Lian, Xin,Ma, Jianmin,Jung, Sun-Min,Jeon, In-Yup,Xu, Jiantie,Baek, Jong-Beom Elsevier 2019 Nano energy Vol.62 No.-

<P><B>Abstract</B></P> <P>Although lithium ion batteries (LIBs) hold great promise as a next generation power supply, the poor rate capability of the graphite that is mainly used as the battery anode limits high-performance LIBs. Compared to other reported carbon-based materials, however, its relatively low average working voltage still makes it attractive. Herein, we were able to introduce carbon disulfide (CS<SUB>2</SUB>) at the edges of graphene nanoplatelets (GnPs) with rich –C=S/-C-S bonds <I>via</I> ball-milling graphite in the presence of CS<SUB>2</SUB>. The resultant edge-thionic acid-functionalized GnPs (TAGnPs) exhibited a larger accessible surface area and smaller particle size than pristine graphite. Importantly, the TAGnPs retained a long-range-ordered layered structure similar to pristine graphite. When the TAGnPs were used as anode materials for LIBs, they displayed superior rate capability (<I>e.g.</I>, high average reversible capacities of 228.3, 208.1, 141.0 and 80.6 mAh g<SUP>−1</SUP> at 0.5, 1, 2 and 5 A g<SUP>−1</SUP>, respectively) compared to pristine graphite and the reference edge-hydrogenated GnPs (HGnPs), which mainly have -C-H bonds at their edges. Theoretical calculations also indicated that the presence of –C=S/-C-S bonds at the edges of TAGnPs enabled stronger Li<SUP>+</SUP> adsorption capability.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Edge-thioated graphene nanoplatelets (TAGnPs) prepared by ball-milling graphite in the presence of carbon disulfide (CS<SUB>2</SUB>). </LI> <LI> TAGnPs have long-range-ordered structure similar to graphite. </LI> <LI> The TAGnPs have a larger accessible surface area than graphite. </LI> <LI> TAGnPs exhibit superior rate capability (>0.5 A g<SUP>−1</SUP>). </LI> <LI> TAGnPs reveal high average reversible capacities of 228.3, 208.1, 141.0 and 80.6 mAh g<SUP>−1</SUP>. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

Hepatic Angiomyolipoma: Contrast Patterns with SonoVue-enhanced Real-time Gray-scale Ultrasonography

Wei, Rui-Xue,Wang, Wen-Ping,Ding, Hong,Huang, Bei-Jian,Li, Chao-Lun,Fan, Pei-Li,Hou, Jun,He, Nian-An Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.2

This study was conducted to retrospectively evaluate the pattern of contrast enhancement with SonoVue on gray-scale ultrasonography of hepatic angiomyolipoma (HAML). Imaging features of 33 pathologically proven HAML lesions in 33 patients who underwent baseline ultrasound and contrast-enhanced ultrasonography (CEUS) were assessed retrospectively. All lesions were enhanced in the arterial phase and showed whole-tumor filling in. Thirty-two of 33 (97%) lesions showed early positive enhancement in the arterial phase. Twenty-three of these exhibited isoechoic or hyperechoic features in the portal phase. HAML demonstrate characteristic manifestations with SonoVue-enhanced real-time gray-scale ultrasonography.

Protectin DX increases alveolar fluid clearance in rats with lipopolysaccharideinduced acute lung injury

Xiao-Jun Zhuo,Yu Hao,Fei Cao,Song-Fan Yan,Hui Li,Qian Wang,Bi-Huan Cheng,Bin-Yu Ying,Bin-Yu Ying,Sheng-Wei Jin 생화학분자생물학회 2018 Experimental and molecular medicine Vol.50 No.-

Acute respiratory distress syndrome is a life-threatening critical syndrome resulting largely from the accumulation of and the inability to clear pulmonary edema. Protectin DX, an endogenously produced lipid mediator, is believed to exert anti-inflammatory and pro-resolution effects. Protectin DX (5 μg/kg) was injected i.v. 8 h after LPS (14 mg/kg) administration, and alveolar fluid clearance was measured in live rats (n = 8). In primary rat ATII epithelial cells, protectin DX (3.605 × 10−3 mg/l) was added to the culture medium with LPS for 6 h. Protectin DX improved alveolar fluid clearance (9.65 ± 1.60 vs. 15.85 ± 1.49, p < 0.0001) and decreased pulmonary edema and lung injury in LPSinduced lung injury in rats. Protectin DX markedly regulated alveolar fluid clearance by upregulating sodium channel and Na, K-ATPase protein expression levels in vivo and in vitro. Protectin DX also increased the activity of Na, K-ATPase and upregulated P-Akt via inhibiting Nedd4–2 in vivo. In addition, protectin DX enhanced the subcellular distribution of sodium channels and Na, K-ATPase, which were specifically localized to the apical and basal membranes of primary rat ATII cells. Furthermore, BOC-2, Rp-cAMP, and LY294002 blocked the increased alveolar fluid clearance in response to protectin DX. Protectin DX stimulates alveolar fluid clearance through a mechanism partly dependent on alveolar epithelial sodium channel and Na, K-ATPase activation via the ALX/PI3K/Nedd4–2 signaling pathway.

Using multi-type sensor measurements for damage detection of shear connectors in composite bridges under moving loads

Xingyu Fan,Jun LI,Hong Hao,Zhi-Wei Chen 사단법인 한국계산역학회 2017 Computers and Concrete, An International Journal Vol.20 No.5

This paper proposes using the multi-type sensor vibration measurements, such as from a relative displacement sensors and a traditional accelerometer for the damage detection of shear connectors in composite bridge under moving loads. Hilbert-Huang Transform (HHT) spectra of these responses will be fused with a data fusion approach i.e., Dempster-Shafer method, to detect the damage of shear connectors. Experimental studies on a composite bridge model in the laboratory are conducted to demonstrate the effectiveness and performance of using the proposed approach in detecting the damage of shear connectors in composite bridges. Both undamaged and damaged scenarios are considered. The detection results with the data fusion of multi-type sensor measurements show a more reliable and robust performance and accuracy, avoiding the false identifications.

Chalcone suppresses lignin biosynthesis in illuminated soybean cells

Chen, Wei-Jun,Yun, Min-Soo,Deng, Fan,Yogo, Yasuhiro The Korean Society of Weed Science and The Turfgra 2011 Weed Biology and Management Vol.11 No.1

Lignin and its related metabolites play critical roles in plant growth and development. Thus, lignin biosynthesis has attracted interest as a novel target site of plant growth inhibitors. Chalcone has been shown to not only inhibit lignin biosynthesis in plants, but also to suppress the growth of many annual plant species. In order to know the direct effect of chalcone on plant metabolism, the effects of chalcone on the activities of key enzymes in lignin biosynthesis and on the related metabolites were clarified with a time-course study by using light-induced suspension cultures of soybean cells.The fresh weight and packed cell volume of the soybean cells were inhibited after 8 h of chalcone treatment. The activities of phenylalanine ammonia lyase (EC 4.3.1.24) and 4-coumarate: coenzyme A ligase (4CL; EC 6.2.1.12) were largely inhibited 4 h after the treatment with 0.15 $mmol\;L^{-1}$ chalcone. Unlike these two enzymes, the activity of cinnamyl alcohol dehydrogenase (EC 1.1.1.195) was not inhibited until 16 h after the chalcone treatment. The content of the 4CL substrates and lignin in the soybean cells became relatively lower than the control under the light condition within 4 h and 8 h after the chalcone treatment, respectively. These results suggest that the growth suppression of soybean cells is positively associated with the inhibition of lignin biosynthesis by exogenous chalcone.

Influences of stochastic perturbation of parameters on dynamic behavior of gear system

Jian-Wei Lu,Fan-Ling Zeng,Jia-Yun Xin,En-Min Miao,Meng-Jun Liu 대한기계학회 2011 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.25 No.7

Gear systems are commonly used in vehicles, and the vibration of the gear system was paid more attention in recent years. In this paper,the dynamic behavior of gear system with stochastic perturbation of system parameters was analyzed. A stochastic nonlinear dynamic model of gear system, with consideration of the stochastic perturbation of system parameters, was established. The influences of stochastic perturbation of system parameters, such as excitation frequency, damping ratio, and backlash, on the dynamic behavior of the system were discussed. It was found that when the perturbation intensity is weak, the topological structure of the system solutions will not change, and there is no transition of the attractors. But if the perturbation intensity increases further, there will be transition between the attractors. In general, for single-DOF gear system, the multi-periodic attractor will jump to the quasi-period-1 attractor. But the quasiperiod-1 attractor will not jump to other attractors. If the perturbation intensity is considerable great, bi-directional transition will occur. Yet, the probability of transition from multi-periodic attractor to quasi-period-1 attractor is greater than the probability of transition from multi-periodic attractor to other attractors. Which provide theoretical basis for effective vibration control of gear system.

New Model of In-situ Xenograft Lymphangiogenesis by a Human Colonic Adenocarcinoma Cell Line in Nude Mice

Sun, Jian-Jun,Jing, Wei,Ni, Yan-Yan,Yuan, Xiao-Jian,Zhou, Hai-Hua,Fan, Yue-Zu Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.6

Objective: To explore a new model of in-situ xenograft lymphangiogenesis of human colonic adenocarcinomas in nude mice. Method: On the basis of establishing subcutaneous xenograft lymphangiogenesis model of human colonic adenocarcinoms, in-situ xenografts were established through the in situ growth of the HT-29 human colonic adenocarcinoma cell line in nude mice. The numbers of lymphangiogenic microvessels, the expression of lymphatic endothelial cell markers lymphatic vessel endothelial hyaloronic acid receptor-1 (LYVE-1), D2-40 and the lymphatic endothelial growth factors vascular endothelial growth factor-C (VEGF-C), -D (VEGF-D) and receptor-3 (VEGFR-3) were compared by immunohistochemical staining, Western bolt and quantitative RT-PCR in xenograft in-situ models. Results: Some microlymphatics with thin walls, large and irregular or collapsed cavities and increased LMVD, with strong positive of LYVE-1, D2-40 in immunohistochemistry, were observed, identical with the morphological characteristics of lymphatic vessels and capillaries. Expression of LYVE-1 and D2-40 proteins and mRNAs were significantly higher in xenograpfts in-situ than in the negative control group(both P<0.01). Moreover, the expression of VEGF-C, VEGF-D and VEGFR-3 proteins and mRNAs were significantly higher in xenografts in-situ (both P<0.01), in conformity with the signal regulation of the VEGF-C,-D/VEGFR-3 axis of tumor lymphangiogenesis. Conclusions: In-situ xenografts of a human colonic adenocarcinoma cell line demonstrate tumor lymphangiogenesis. This novel in-situ animal model should be useful for further studying mechanisms of lymph node metastasis, drug intervention and anti-metastasis therapy in colorectal cancer.

Next-generation sequencing of the mitochondrial genome of Dolichovespula panda (Hymenoptera: Vespidae) with a phylogenetic analysis of Vespidae

Xu-Lei Fan,Ya-Jun Gong,Peng-Yan Chen,Qing-Qing Tan,Jiang-Li Tan,Shu-Jun Wei 한국응용곤충학회 2017 Journal of Asia-Pacific Entomology Vol.20 No.3

For the first time the mitochondrial genome of a Dolichovespula species, D. panda Archer (Hymenoptera: Vespidae), was sequenced with a next-generation sequencing approach. The sequenced mitochondrial genome is 17137 bp long and consists of 13 protein-coding, 22 tRNA and two rRNA genes, as well as a partial A +T-rich region. Twenty-two of the genes are encoded on the majority strand and 15 genes on the minority strand. All protein-coding genes start with ATN codons and have a TAA termination codon, except for one with a TA codon. Compared with the putative ancestral arrangement of insects, the D. panda mitochondrial genome shows the shuffling of trnN and trnE, and of trnQ and trnM, the translocation of trnY to upstream of trnI, and of trnL1 to the region between trnS2 and nad1 and a reversal of trnS1. A phylogenetic tree within the Vespidae was reconstructed using the 13 protein-coding mitochondrial genes. This shows a sister group relationship between Dolichovespula and a clade formed by Vespa and Vespula. It also corroborated the position of Eumeninae as sister group of the clade Polistinae +Vespinae.

Distinct mutations in MLH1 and MSH2 genes in Hereditary Non-polyposis Colorectal Cancer (HNPCC) families from China

( Wen Qian Wei ),( Fang Qi Liu ),( Lei Liu ),( Zuo Feng Li ),( Xiao Yan Zhang ),( Fan Jiang ),( Qu Shi ),( Xiao Yan Zhou ),( Wei Qi Sheng ),( San Jun Cai ),( Xuan Li ),( Ye Xu ),( Peng Nan ) 생화학분자생물학회(구 한국생화학분자생물학회) 2011 BMB Reports Vol.44 No.5

Hereditary non-polyposis Colorectal Cancer (HNPCC) is an autosomal dominant inheritance syndrome. HNPCC is the most common hereditary variant of colorectal cancer (CRC), which accounts for 2-5% CRCs, mainly due to hMLH1 and hMSH2 mutations that impair DNA repair functions. Our study aimed to identify the patterns of hMSH2 and hMLH1 mutations in Chinese HNPCC patients. Ninety-eight unrelated families from China meeting Amsterdam or Bethesda criteria were included in our study. Germline mutations in MLH1 and MSH2 genes, located in the exons and the splice-site junctions, were screened in the 98 probands by direct sequencing. Eleven mutations were found in ten patients (11%), with six in MLH1 (54.5%) and five in MSH2 (45.5%) genes. One patient had mutations in both MLH1 and MSH2 genes. Three novel mutations in MLH1 gene (c.157_160delGAGG, c.2157dupT and c.-64G>T) were found for the first time, and one suspected hotspot in MSH2 (c.1168C>T) was revealed. [BMB reports 2011; 44(5): 317-322]