마우스 CT26 종양에서 Tc-99m 표지 플루란유도체의 저류 특성

허영준,송호천,범희승,나건,김성민 대한핵의학회 2003 핵의학 분자영상 Vol.37 No.6

배경: 플루니유도체(PD)로 만든 나노입자는 이온강도에 다라 뭉치는 수용성겔이다. 본 연구에는 PD가 방사성핵종을 종양 조직 내에 효과적으로 머무르게 할 수 있는지 알아보고자 하였다. 방법: 네 종류의 PD 즉, pullulan acetate (PA), succinylated PA(SPA), PA-DTPA와 SPA-DTPA 결합체를 합성한 후 테크네슘-99m(Tc-99m)으로 표지하고 표지효율을 측정하였다. Balb/c 생쥐에 CT26 대장암세포를 피하주사하고 2주 후에 Tc-99m 표지 PD(Tc-PD)를 종양 내에 주사하였다. 주사직후와 30분, 1, 2, 4, 12시간 후에 감마카메라로 생쥐를 촬영하여 종양 내의 Tc-PD의 저류율을 측정하였다. 종양크기에 따라 각각의 암의 직경이 5mm와 10 mm일대 Tc-99mper-technctate와 Tc-99m 표지 PA를 종양 내 주사하여, 주사 직후 1시간 동적영상을 얻고, 1시간, 2시간, 3시간 그리고 4시간 후에 감마카메라로 생쥐를 촬영하여 종양 내의 저류율을 측정하였다. 결과: PA, SPA, PA-DTPA 및 SPA-DTPA의 Tc-99m 표지율은 각각 94.5±5.9%, 97.8±3.5% 94.2±3.8%, 92.5±62.%로 서로간에 유의한 차이가 없었다 (p>0.05). Tc-99m-PA와 Tc-99m-PA-DTPA의 %RR은 대조군에 비해 의의있게 높았으나(p<0.05), SPA는 4시간까지 그리고 SP-DTPA는 2시간가지만 대조군보다 %RR이 높았을 뿐 그 이후에는 대조군과 유사한 %RR을 보였다. 종양의 크기에 따라 PA군에서 측정한 저류율은 대조군보다는 의의있게 높았으나, 직경이 5 mm와 10 mm일 때의 저류율 간에는 차이가 없었다. 결론: PD가 종양 조직 내에 저류될 수 있음을 알았으며, 종양 크기에 따른 저류율의 차이는 없었다. 향후 PD와 치료용 방사성핵종을 이용한 종양치료에 활용할 가능성을 기대할 수 있을 것으로 사료되었다. Objective: Pullulan derivatives (PD) can be used to make self-assembled hydrogel nanoparticles which are responsive to ionic strength. The aim of this study is to evaluate the potential of PD as a retaining carrier of radioisotope inside tumors. Materials and Methods: Four types of PD were evaluated which included pullulan acetate (PA), succinylated PA (SPA), PA-DTPA and SPA-DTPA conjugates. They were radiolabeled with Tc-99m. Labelling efficiencies were determined at 30 min, 1, 2, 4 and 12 hours after radiolabeling. CT-26 colon cancer cells were subcutaneously injected into Balb/c mice. After 2 weeks of subcutaneous injection, Tc-99m-labelled PD (Tc-99m-PD)were injected into the tumors. Whole body images of mice were obtained at 30 min, 1, 2, and 12 hr after intratumoral injection. All twenty mice were grouped into four groups by largest diameter: control A (largest diameter = 5 mm, n = 5), control B (largest diameter = 10 mm, n = 5), pullulan A (largest diameter = 5 mm, n = 5), pllulan B (largest diameter = 10 diameter = 10 mm, n = 5), pullulan A (largest diameter = 5 mm, n = 5), pllulan B (largest diameter = 10mm, n = 51. Dynamic images were obtained for 1 hour after intratumoral injection. Static images were obtained at 1 hr, 2 hr, 3 hr and 4 hr after intratumoral injection with Tc-99m pertechnetate and Tc-99m-PA. Target-to-background ratios and retention rates were calculated. Results: Labeling efficiencies of PA, SPA, PA-DTPA and SPA-DTPA were 94.5 ±5.9%, 97.8±3.5% 94.2±3.8%, and 92.5±6.2%, respectively (p>0.05). Percent retention rates (%RR) of PA and PA-DTPA were significantly higher than those of control, however, those of SP-DTPA and SPA became similar to control at 4 and 12 hr, respectively. %RR of pullulan A and pullulan B at 1, 4 and 8 hr is significantly higher than that of control (p<0.051. However, %RR between pullulan A and pullulan B were similar. Conclusion: The ionic strength dependent PD-nanoparticles are retained in the tumor. No difference of %RR according to tumor size was noted. Therapeutic application of PD labelled with beta- or alpha- emitting radionuclides can be expected.

3차 병원의 병동에서 시행된 심폐소생술의 분석

류진호,정경운,위준선,문정미,전병조,문원식,김용권,소정일,허탁,민용일 대한응급의학회 2001 대한응급의학회지 Vol.12 No.4

Background: Although cardiopulmonary resuscitation(CPR) is a very effective therapy in cardiac arrest, it is hard to prove the true effectiveness of CPR. Several studies about out-of-hospital and emergency department CPR exist, but only a few reports about in-hospital CPR are available. This study was designed to investigate in-hospital cardiac arrest, to analyze the result of CPR, and to evaluate the problems associated with in-hospital CPR. Methods: A clinical analysis of 71 cases of in-hospital CPR announcement from January 2000 to August 2000 was performed. The initial rhythm on cardiac arrest, return of spontaneous circulation(ROSC), and the survivals were analyzed in the case of the 46 true cardiac arrest patients. Results: During 8 months, there were 71 cases of in-hospital CPR announcement. Among them, there were 46 cases of true cardiac arrest and 25 cases of non-cardiac arrest. Of the 46 true cardiac-arrest cases, 27(58.7%) experienced ROSC, 15(32.6) survived for over 24 hours, and 7(15.2%) survived to be discharged. The initial rhythms on cardiac arrest were 30 cases(65.2%) of asystole, 14(30.4%) of PEA(pulseless electrical activity), and 2(4.3%) of ventricular fibrillation, with ROSC being 17 cases(56.7%), 9(64.3%) and 1(50.0%) cases and discharged survivors being 4 cases(13.3%), 3(21.4%) and 0(0.0%) cases, respectively. Conclusion: Extraordinarily high proportions of asystole and PEA were seen in the initial rhythm of cardiac arrest, and those were associated with high survival rates. Although further study is needed to evaluate the course leading to this high proportion of asystole and PEA, this result suggests that if the EMS system in the hospital is activated promptly and systematically, a better outcome will be achieved in case of cardiac arrest with asystole and PEA.

하악골 골절 환자에 있어 수술후 합병증으로 발생한 골수염의 치험례

윤규호,전인성,신용길,박준호,허남오,방석준 大韓顎顔面成形再建外科學會 1996 Maxillofacial Plastic Reconstructive Surgery Vol.18 No.4

The term osteomyelitis literally means inflammation of bone marrow. It is described as an inflammatory condition of bone primarily engaging the medullary Following circulatory collapse and ischemia, the involved portions of the bone become necrotic. Cellulitis and osteomyelitis are the most frequent complications of fractures of the mandible and maxilla, because there is an abscessed tooth in the line of fracture. Since most fractures are compound fractures, infection may develop because of contact with oral secretions or outside air. The treatment of chronic osteomyelitis is still under debate. Some authors rely on antibiotics alone, while others advocate combination with surgery. But when infection occurs, establish proper drainage and administer antibiotics. Author treated a case of infected mandibular fracture with mandibular osteotomy, sequestrectomy, iliac bone graft and hyperbaric oxygen therapy, who had suffered suppurative osteomyelitis and mandibular nonunion following the open reduction of the mandibular fracture.

치과용 아말감의 열반응에 관한 연구

최선진,허준호,김철위 大韓齒科器材學會 1990 대한치과재료학회지 Vol.17 No.1

The aim of the present investigation was assess the thermal reaction during hardening of representative fourteen commercially available conventional dental amalgam alloys: DA HA HV KA AC CA FC IH MM OT SS SCA SA UT and six high-copper dental amalgam alloys: A-21 KS LA OP PM PA used in Korea. These tests were performed with a thermistor probe (YSI Model 42 SC) using physiograph(MK-IV, Narco, Biosystems, U.S.A.). The peak temperature of the thermal reactions of dental amalgams were determined by the mean value of three measurements. From the experiments, the results were as follows: 1. The results indicate that the peak temperature of exothermic reactions of the conventional amalgams was attained during 7.67 ±1.76℃ and 15.50 ±0.50℃, respectively. 2. The results suggest that the exothermic peak temperature for the high-copper amalgams was considerably higher than those of the conventional amalgams. 3. The results for the recommended ratio revealed significant increases in exothermic reactions and the (+) modified ratio showed significantly less exothermic reactions than the (-) modified ratio. 4. The results show that the peak temperature rise of dental amalgams was attained during 7.17 ±0.29℃ and 24.17 ±0.29℃ according to the type of amalgamators, respectively.

치과용 앨지네이트의 국산화를 위한 규격 및 안전성에 관한 연구

김철위,허준호 大韓齒科器材學會 1990 대한치과재료학회지 Vol.17 No.1

The purpose of this study was to investigate the setting time, viscosity, surface detail reproduction, working time, permanent deformation, strain in compression, compressive strength, temperature rise during gelation, differential thermal analysis, acidity and toxicological properties of representative fourteen commercially available dental alginate impression materials (AG, AP, AR, CC, ID-Ⅰ, ID-Ⅱ, JT, KG, PG, PC-I, PC-Ⅱ, SM-I, SM-Ⅱ and ZG) and three experimental dental alginate impression materials(Exp A, Exp B and Exp C). These tests were performed by the methods of KDA specification No. 8, ISO specification No. 1563 and ANSI/ADA specification No. 18. Form the experiments, the following results can be drawn: 1. The setting time of the fast setting types showed considerable differences according to the products, but there was no significant difference in setting time of the normal setting types. The significantly difference of viscosity values for the normal setting types was observed with various commercial products, but there was no significant difference for the fast setting types. 2. The reproduction of surface detail of the normal setting types was higher than that of the fast setting types, and the working time of the alginate impression materials showed considerable difference according to the products. 3. The permanent deformation of the fast setting types was lower than that of the normal setting types, and the strain in compression, the compressive strength, and the temperature rise during gelation of alginate impression materials showed considerable differences according to the products. 4. The rapid endothermic temperature curves of the alginate impression materials proceed at 127.0℃∼134.7℃, and the exothermic temperature curves showed at 327.7℃∼367.0℃ 5. The acidity of the alginate impression materials showed considerable differences according to the products, and the fast setting types was lower than that of the normal setting types. 6. The results indicated that the dental alginate ingredients did not increased the frequencies of micronucleated polychromatic erythrocytes when compared with negative controls.

응급실에 내원한 환자에서 어지럼증의 원인과 임상양상의 분석

문원식,정경운,위준선,문정미,전병조,김용권,소정일,류진호,허탁,민용일 대한응급의학회 2001 대한응급의학회지 Vol.12 No.3

Background: Recently, The number of patients who have been complaining of the vertigo or dizziness has been increasing due to rapid growth of elderly population and senile disease. The differentiation of dizzy patients is not familiar to most emergency physicians. This study was designed to differentiate true vertigo and to investigate the clinical difference among central vertigo, peripheral vertigo, and other causes of dizziness. Methods: The authors analyzed the cases of 237 dizzy patients who visited the emergency department of Chonnam University Hospital during the recent 2 years. For the base of dizziness, associated past illnesses, severity, nystagmus type, and the causes of central and peripheral vertigo. Results: Female patients were 142(59.9%) and male patients were 95(40.1%). The most common age group was the 6th decade. The origin of dizziness was classified as peripheral cause(25.3%), central cause(32.9%), and others(41.8%). As to nature of the dizziness, the rotatory sense was dominant in peripheral vertigo and the floating sense and blurred vision in central vertigo. Peripheral vertigo was triggered by position change of head and body, and central vertigo by the eye movement. Audiograms I showed that most patients with central vertigo had normal hearing, but 46.7% of thoswith peripheral vertigo had an abnormal hearing disturbance. Nystagmus was more prevalent in peripheral vertigo Conclusion: History taking and physical examination played an important role in the diagnosis of dizzy patients. An important part of the diagnosis of a dizzy patient could be to evaluate the peripheral origin, the central origin and others causes. Because central vertigo may be associated with a significant neurological pathology, spectral radiological studies, including MRI and CT, are mandatory to rule out a devastating brain lesion.

급성 및 만성 간질환에서 C형 간염바이러스 항체(anti-HCV)양성률

김삼용,성자원,김병호,이기천,허승식,길준영,정현용,이헌영,김영건 충남대학교 의과대학 지역사회의학연구소 1992 충남의대잡지 Vol.19 No.2

The prevalence of antibody against hepatitis C virus (anti-HCV) was investigated in 331 patients with various liver diseases from April 1992 to September 1992. The presence of anti-HCV was detected by Lucky HCD EIA test in 331 cases and by (Ortho HCV Antibody ELISA test 148 patients) The results are summarized as follows: 1. Overall, antibody to HCV(anti-HCV) was positive in 37(11%) of 331 patients with various liver diseases. 2. 16(11%) of 144 patients with chronic hepatitis, 2(5%) of 37 patients with alcoholic liver disease, 17(22%) of 76 patients with liver cirrhosis and 2(5%) of 38 HBsAg carriers were positive for anti-HCV.None of 12 patients with acute hepatitis, 11 patients with drug induced hepatitis and 13 patients with hepatocellular carcinoma was anti-HCV positive. 3. HBsAg positivity in patients who had anti-HCV was 24%(9/37). 4. A positive correlation was found between Lucky HCD and Ortho HCV test. Of 128 Lucky HCD negative cases, 124 cases were negative for Ortho HCV ELISA and 4 cases were positive for Ortho HCV ELISA Of 20 Lucky HCD positive cases, 11 cases were positive for Ortho HCV ELISA and 9 cases were negative for Ortho HCV ELISA. These results suggest that hepatitis C virus has an important etiologic role in HBsAg negative chronic hepatitis and liver cirrhosis in Korea. The diagnostic value of Lucky HCD EIA test may be slightly better or equivalent to Ortho HCV ELISA test in the diagnosis of Hepatitis C virus infection.

Development of New Efficient Synthetic Methods for Docetaxel

Heo, Jae-Ho,Park, Sung-Jun,Kang, Joo-Hi,Lee, In-Suk,Lee, Jin-Seo,Park, Young-Jun,Kim, Kyoung-Soo,Lee, Jae-Yeol Korean Chemical Society 2009 Bulletin of the Korean Chemical Society Vol.30 No.1

Purification and characterization of sulfated polysaccharides from Sargassum binderi with inhibitory mechanism for iNOS and COX-2 pathway interaction

Jun-Ho Heo,Jun-Geon Je,Hyo-Geun Lee,Jimin Hyun,Sang-Woon Lee,So-Yeon Park,You-Jin Jeon,BoMi Ryu 한국수산과학회 양식분과 2022 한국수산과학회 양식분과 학술대회 Vol.2022 No.5

Utilization of Pectinase Cocktail and High Hydrostatic Pressure for the Production of Aged Black Garlic Juice with Improved Nutritional Value

Jun Ho Kim,Jin Woo Kim,Su Hyun Yu,Jonghwa Lee,Hyung Taek Cho,Wan Heo,Sung Jin Park,Jin Hyup Lee,Young Jun Kim 한국식품영양과학회 2019 Preventive Nutrition and Food Science Vol.24 No.3

In comparison with raw garlic, aged black garlic has been shown to display multiple pharmacological activities. We recently reported that pretreatment of pectinase cocktail with high hydrostatic pressure (HHP) before the process of aging garlic juice improves its antidiabetic activity and increases S-allylcysteine (SAC) content. Thus, this study was designed to investigate the influence of pectinase cocktail with HHP on the quality of aged black garlic juice formation and to identify the optimal manufacturing conditions. In the pretreatment step, garlic juice is heated at 55℃ for 24 h. The contents of SAC and total polyphenols were increased with treatment of pectinase cocktail; this increase was greater under HHP processing. In contrast, the total flavonoid content was decreased in all pretreatment conditions. Garlic juice pretreated with pectinase cocktail and HHP had a significantly higher content of SAC in the early phase of aging than raw garlic juice, and the SAC was increased over time in both treatment groups. The total polyphenol content of garlic juice was significantly higher in the pretreatment group during the aging period, and the antioxidant activity of garlic juice showed a positive correlation with polyphenol content. Interestingly, HHP increased the enzymatic activity of the pectinase cocktail.