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      • KCI등재

        Two New Phenolic Glycosides from Curculigo orchioides

        Ai-Xue Zuo,Yong Shen,Zhi-Yong Jiang,Xue-Mei Zhang,Jun Zhou,Jun ,Ji-Jun Chen 대한화학회 2011 Bulletin of the Korean Chemical Society Vol.32 No.3

        Two new phenolic glycosides were isolated from the rhizomes of Curculigo orchioides Gaertn.. Based on comprehensive spectroscopic analyses including IR, MS, 1D- and 2D NMR (COSY, HSQC, and HMBC), their structures were elucidated as 3-hydroxyl-5-methyphenol-1-O-[β-D-glucopyranosyl-(1→3)-β-D-glucopyranoside (1) and 1',3'-dimethoxyl-4-hydroxyalangifolioside (2).

      • KCI등재

        An Analysis of Trace Information of Different-shaped Fracture Networks Having a Same Fracture Intensity (P32)

        Jichao Guo,Jun Zheng,Qing ,Zhipeng Xiao,Tiexin Liu 대한토목학회 2022 KSCE JOURNAL OF CIVIL ENGINEERING Vol.26 No.10

        Discrete fracture network (DFN) simulation is the basis of studying the properties of a rock mass. An important premise of constructing three-dimensional (3-D) DFNs is to ensure that the fracture intensity (P32) of the simulated rock mass equals to that of the real one. In fact, the natural fracture shapes are various, and we can only obtain one-dimensional (1-D) and two-dimensional (2-D) measurements of a rock mass and usually infer 3-D DFNs based on 1-D and 2-D measurements. The following question would be induced: whether the DFNs with the same P32 and different shapes have similar 2-D trace information. Hence, a series of hypothetical 3-D elliptical DFNs with different values of long-short axis length ratio ke are constructed, and disc models are used to represent them. The algorithms of obtaining traces on sampling windows concerning circular and elliptical fractures are developed. The results show that as the values of ke increase, the error rate of the trace numbers and lengths between the elliptical fractures and simulated disc models on the same sampling windows increases. Moreover, because of the significant impact of fracture shape, the Accuracy representation Index is proposed to better evaluate which model is more appropriate for constructing DFNs.

      • KCI등재

        Preparation of HMX by Catalytic Nitrolysis of DPT in AIL-N_2O_5-HNO_3 System

        Zhi-yong He,Jun Luo,Chun-xu ,Ping Wang,Rong Xu,Jin-shan Li 대한화학회 2011 Bulletin of the Korean Chemical Society Vol.32 No.8

        Direct nitrolysis of 3,7-dinitro-1,3,5,7-tetraazabicyclo[3,3,1]nonane (DPT) is a feasible way to synthesize HMX, and it has multiple practical applications. In this paper, a new nitrolysis process involving the use of an N_2O_5-HNO_3 system catalyzed by acidic ionic liquids (AILs) was developed. The results show that [Et_3NH]TsO was the best catalyst among the 28 AILs used and that HMX was formed at a higher yield of 61%, compared to 45% without any AIL. Moreover, with the addition of N_2O_5, the yield was further increased to a maximum value of 69%. The AILs were also efficiently recovered by simple extractions without any apparent loss of catalytic activity, even after five runs.

      • KCI등재

        Generation of Selenoprotein with Glutathione Peroxidase Activity by Chemical Modification of the Single-chain Variable Fragment Expressed in a Single-protein Production System and Its Antioxidant Ability

        Cheng Wang,Gang-Lin Yan,Shao-Wu ,Chun-Hong Sui,Yang Zhao,Ya-Wei Xu,Gang Zhao,Jun-jie Xu,Ping-Sheng Gong,Gui-Min Luo,Ying Mu 한국생물공학회 2013 Biotechnology and Bioprocess Engineering Vol.18 No.1

        Glutathione peroxidase (GPX) is one of the important members of the antioxidant enzyme family. It can catalyze the reduction of hydroperoxides with glutathione to protect cells against oxidative damage. Single-chain variable fragment (scFv) can be converted into seleniumcontaining single-chain variable fragment (Se-scFv) by chemical modification of the hydroxyl groups in scFv, thus Se-scFv possesses GPX activity and becomes a prodrug. To improve the expression of scFv and simplify its purification steps, Single-protein production (SPP) system was used to express scFv and chemical modification was used to synthesize Se-scFv. Therefore, we must construct a new scFv-WCD1-lessACA gene, which can express its mRNA not containing any ACA sequences and express its amino acid sequence of target protein (scFv) being same to scFv-WCD1. In this way, the scFv-WCD1-lessACA can be only expressed in SPP system and no other background proteins in the cells could be expressed. The expression results showed that high level of scFv-WCD1-lessACA synthesis was at least sustained for 96 h in the virtual absence of background protein synthesis. Then, selenocysteine (Sec) was incorporated into the scFv-WCD1-lessACA by chemical modification and resulted in Se-scFv-WCD1-lessACA. The enzymatic characteristics of Se-scFv-WCD1-lessACA were determined. GPX activity was 2,563 U/μmol,its binding constant for GSH was 0.687 ×105/mol. Moreover,Se-scFv-WCD1-lessACA was confirmed to have a strong antioxidant ability to protect mitochondria against oxidative damage induced by Vc/Fe2+ (mitochondrial damage model),suggesting that Se-scFv-WCD1-lessACA has potential application for protection of mitochondrial damage induced by reactive oxygen species (ROS).

      • KCI등재

        Molecular characterization and ligand-binding properties of six odorantbinding proteins (OBPs) from Aphis gossypii

        Xue-Ke Gao,Shuai Zhang,Jun-Yu Luo,Chun-Yi Wang,Li-Min ,Li-Juan Zhang,Xiang-Zhen Zhu,Li Wang,Jin-Jie Cui 한국응용곤충학회 2018 Journal of Asia-Pacific Entomology Vol.21 No.3

        Odorant-binding proteins (OBPs) play a significant role in the olfactory signal transduction of insects and help them locate hosts, oviposition sites, and mating partners. The sap-sucking insect, Aphis gossypii Glover (Homoptera: Aphididae), is a destructive cosmopolitan pest and yet the molecular mechanisms by which A. gossypii perceives pheromones and host volatiles remain unknown. In this study, we identified and characterized 6 OBPs, using the A. gossypii RNA-seq transcriptome dataset previously constructed in our laboratory. Real-time PCR indicated specific expression patterns of the 6 genes, which had different levels of expression based on development stage, tissue, morph, and life cycle. In addition, binding specificities of the 6 proteins investigated using the ligand-binding assays showed that all 6 OBPs exhibited high binding affinities towards Phlorizin dehydrate while AgosOBP3 and AgosOBP4 had strong affinity to beta-ionone and AgosOBP8 displayed higher binding affinities for Nerolidol and Cis-3-hexenyl acetate compared to other OBPs.

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