Streptococcus mutans와 Lactobacillus acidophilus에 대한 Polylysine의 항균활성

전주연,이갑상,신용서 한국식품영양학회 1999 韓國食品營養學會誌 Vol.12 No.2

단백질성의 치아우식 예방제를 개발하기 위해 polylysine을 선정하고 Streptococcus mutans와 Lactobacillus acidophilus의 생육과 산생성량에 미치는 영향을 anaerobic broth system에서 미생물학적인 방법에 준하여 조사하였으며, polylysine의 항균활성에 대한 pH와 열 안정성을 조사하였다. Streptococcus mutnas와 Lactobacillus acidophilus의 생육은 polylysine의 첨가농도가 증가할수록 감소하였으며 1.0㎎/ml 이상의 첨가농도에서는 두 시험균의 lag time이 약 1시간 정도 연장되는 효과가 있었다. 시험균의 산 생성량도 polylysine에 의해 감소되었으며 특히 1.0㎎/ml의 첨가농도에서 그 효과가 뚜렷하였다. Polylysine은 pH 5.0∼8.0에서도 그 항균활성이 유지되었으며, 60℃에서 30분간, 120℃에서 15분간의 열 처리에도 그 항균활성이 소실되지 않았다. To evaluate antidental caries activity of polylysine, cell growth and acid production of Streptococcus mutans and Lactobacillus acidophilus were microbiologically monitored in anaerobic broth system containing various concentration of polylysine. The pH and heat stability of polylysine having antimicrobial activity were also examined. Two tested microbes were fairly well grown in broth containing polylysine 0.1㎎/ml, however, inhibited at 1 and 2 ㎎/ml of polylysine concentration. Especially, lag times of Streptococcus mutans and Lactobacillus acidophilus were prolonged to about hour at 1.0 and 2.0 ㎎/ml of polylysine. Acid production of Streptococcus mutans and Lactobacillus acidophilus was also decreased by polylysine. Antimicrobial activity of polylysine was not affected by the change of pH and the heat treatment.

분산 컴퓨팅 환경과 미들웨어

조성연,장주만,전병태 高麗大學校附設 컴퓨터科學技術硏究所 1999 JOURNAL OF COMPUTER SCIENCE & ENGINEERING TECHNOLO Vol.1 No.-

미들웨어라는 개념은 클라이언트/서버 환경에서 클라이언트와 서버를 연결해 주는 역할을 하는 모든 어플리케이션들을 포함한다. 그러나 단순히 클라이언트와 서버를 연결시켜 주는 것만으로 미들웨어라고 할 수 있는 것은 아니다. 클라이언트의 서비스 요구를 서버에게 전달하고 요구 사항을 처리한 서버의 결과를 클라이언트에게 전달하는 과정의 수행을 책임진다. 그 과정에서 실제 서비스의 처리나 결과를 사용자에게 보여주는 과정은 서버와 클라이언트의 몫으로 제외된다. 미들웨어는 분산 환경에서 확장성과 개방성을 제공하는 핵심 부분이다. 미들웨어의 기본 기능인 투명성이외의 통신 기능, 스케줄링 기능, 트랜잭션의 기능 등등의 많은 서비스를 제공한다. 현재 미들웨어는 제공하는 기능의 특징에 따라서 종류가 다양하다. 본 논문에서는 미들웨어의 범위를 정의하고 현재 개발되고 있는 미들웨어들인 RPC, ORB, MOM을 분석하고 비교한다. Middleware covers all softwares that connect client and server. but middleware not only do connection. it takes responsible all through process from that client requests service to that server returns result except real processing of service. middleware is essential of distributed environment. middleware provides communication function, thread scheduling function, transaction function etc. there are different characteristic middleware according to characteristics. this article defines what is middleware and compares different middlewares with another especially PRC, ORB, MOM.

20대 여성의 치아우식활성검사 결과와 치아우식경험도와의 상관성에 관한 연구

양정승,전주연,이현옥 광주보건대학 1994 論文集 Vol.19 No.-

The purpose of this study was as follows: This study was to examine caries activity test and survey caries experience of 120 girl students in college. We examined correlation the results of caries activity test and caries experience. First of all, examined Snyder test, stimulated saliva flow rate test, unstimulated saliva flow rate test, salivary viscosity test, patient oral hygiene performance test. We surveyed cries eperience as caries experienced permanent tooth index and caries experience permanent tooth rate etc, calculated the simple correlation index, examined statistical significant. The results of this study showed that stimulated saliva flow rate test was the highest of caries activity test measurement. In caries activity test statistical significant correlation. It was the correlation index- 0.2136 and p=valuable level 0.010. Snyder Test had correlation, was the correlation index 0.1779, and p-value level 0.026. There was no significant correlation unstimulated saliva flow rate test and patient oral hygiene performance test.

노인들의 구강건강 관리실태에 관한 연구

박정순,박인숙,전주연 韓國保健敎育 ·健康增進學會 1999 보건교육건강증진학회지 Vol.16 No.2

For studying on the actual condition of the oral health control of the old there were invastigations on the questions with the college of the old in Chonbuk as the central figure from June 1 to June 30, 1998 and the analyses about the frequency and the mutual relation through χ²-test. The results are as follow: 1. According to the result of the investigation into the actual condition about the oral health of the old, two times a day in the frequency of tooth brush per day was the highest at the rate of 50.35%, the use of dentifrice was 45.4%, and the exchanging time of tooth-brush after six months was the highest at the rate of 19.7%. And 96.1% didn't use dental floss and 73.6% didn't do scaling. It is thought that since 50% used tooth sticks and match sticks we have to inform the old of the necessity of dental plague and period oral examination. 2. In oral health situation, it showed that the number of people who were very week was the highest at the rate of 37% and there was the mutual relation between the use of tooth sticks and match sticks and present situation of oral health. And 54.93% visited the dental clinic wethin a year, and 22.3% among them made full denture. There was the mutual relation between the visit of dental clinic within a year and the consultation content and the number of people who teld that the consultation expense was very expensive was the higest at the rate of 27.8% and 87% didn't experience the oral education. So it is thought that we have to make an systematic education about the oral to the old and inform them of the necessity of oral health control. 3. We think it needs the establishment and plane of the systematic basis material and the oral education which is necessary in oral public industry for the dental preventive suitable to the characteristic of the old.

Antimicrobial Activity of Polylysine against Oral Bacteria

Chun, Ju Yean,Park, Chung Soon,Shin, Yong Seo 대한구강보건학회 1997 大韓口腔保健學會誌 Vol.21 No.5

Anticaries Activity of Antimicrobial Material from Bacillus alkalophilshaggy JY-827

(Ju Yean Chun),(Il Hwan Ryu),(Jung Sun Park),(Kap Sang Lee) 한국미생물생명공학회 2002 Journal of microbiology and biotechnology Vol.12 No.1

N/A N/A

치위생과 학생의 자기표현이 임상실습 스트레스에 미치는 영향

전주연 ( Ju Yean Chun ),이현옥 ( Hyun Ok Lee ),김진 ( Jin Kim ) 한국치위생과학회 2007 치위생과학회지 Vol.7 No.2

Bacillus alkalophilshaggy JY-827가 생산하는 항우식균물질의 생산조건과 분리, 정제 및 특성

전주연,류일환,이상욱,이갑상 한국산업미생물학회 2000 한국미생물·생명공학회지 Vol.28 No.5

분리균주 Bacillus alkalophilshaggy JY-827가 생산하는 항균물질의 생산을 최적 배양 조건 및 배지조성은 각각 25℃, pH 11, glucose 1.5%(w/v), pepton 1.0%(w/v), K_2HPO_4 0.2%(w/v)였으며, 기본배지와 최적배지에서의 항균물질 생산은 피검균 S. mutans에 대하여 18.0과 24.3mm로 나타나 항균물질의 생산은 기본배지에 비해 최적배지에서 생산시 약 1.5배 정도 증가되었다. 또한 균주 배양 상등액에 ehylacetate(EtOAc) 추출, silica gel column chromatography, sephadex LH-20 column chromatography, MeOH 재결정후 융점 152-154℃이었고, rm [α]^20_D은 +55˚의 환상의 결정을 갖는 백색분말을 얻었다. IR, ^1H-NMR, ^13C-NMR을 통하여 분석하여 본 결과 amino group을 갖는 glucoside로 판단되었다. 분리정제된 aminoglycoside antibiotic은 물이나 methanol과 같은 극성 용매에 잘 용해되는 반면, ehter, chloroform과 같은 비극성 용매에 잘 용해되지 않는 결과를 보였다. S. mutans JC-2에 대한 항균력(MIC value)은 2.5×10 exp (-7) M이었다. 온도 및 pH 안정성은 각각 70℃까지 안정하였으며, pH 6-10의 알칼리영역에서 비교적 안정한 반면, pH 5.0이하의 산성영역에서는 잔존활성이 급격히 감소하는 결과를 나타내었다. The study was performed to investigate the excellent microbial anticaries substance which is more effective than the chlorhexidine in the dental caries treatment. A typical strain which produced the most excellent antimicrobial substance was selected. and identified novel alkalophillic Bacillus alkalophilshaggy JY-827. For the maximal production of the microbial antibiotic against Streptococcus mutants from B. alkalophilshaggy JY-827, the optimal culture condition was in the medium containing glucose 15 g/L, peptron 10 g/L and K_2HPO_4 2 g/L the highest production of antibiotic against S. mutans was obtained at 25℃ and pH 11.0 for 5 days. The antibiotic from B. alkalophilshaggy JY-827 was purified by organic solvent extraction, silica gel and sephadex LH-20 column chromatograpies, and then crystallized with methanol. The crystallin compomation of this antibiotic was as a curcular shape. The melting point and rm[α]^20_D were 152-154℃ and +55˚, respectively. Based on Instrumental anylyses such as FT-IR, ^1H-NMR, ^13C-NMR and GC-mass, the antibiotic was identified as aminoglycoside. It was obtained as amorphous white powder, and soluble in water, methanol but insoluble in ether, chroloform. This antibiotic inhibited the growth of S. mutans to about 3 day at the concentration of 2.5×10 exp (-7) M. It was stable at the alkalli condition but unstable within the acid condition. It was also stable up to 70℃.

치아 우식 미생물 Streptococcus mutans에 대해 항균활성을 갖는 신규 호알칼리성 Bacillus alkalophilshaggy JY-827의 분리 및 동정

전주연,류일환,이상욱,이갑상 한국산업미생물학회 2000 한국미생물·생명공학회지 Vol.28 No.5

Streptococcus mutans 생육 저해 물질 생산균주를 선별하기 위하여 갯벌 토양으로부터 S. mutans에 대하여 저지환을 나타내는 1,150 균주 중 1차 선별하였고, 이중 가장 큰 저지환의 크기를 나타낸 JY-827 균주를 선별하여 제 특성을 확인하여 동정한 결과, 분리균주 JY-827의 colony 형태는 원형이었고, 색깔은 유백색이었으며 표면은 볼록하고 매끄러운 상태였다. 호기성이였으며, 균체의 침전현상은 보이지 않았다. 이 균주의 형태학적 특성은 구균으로서 그 크기는 200×250㎛으로 비교적 size가 큰편이였으며, 편모는 수많은 편모가 균체 전체를 덮고 있는 형태였으며, 운동성이 관찰되고, Gram 양성이였다. 생육범위는 pH 9.0~12.0이었고, 생육온도 범위는 15~45℃이었다. 분리균주 JY-827의 세포 지방산 성분을 검토한 결과, 주성분은 iso-15:0과 anteiso-15:0인 branched type으로 각각의 함량은 40.09%와 28.92%를 나타내었다. G+C mole의 함량은 약 55%로 나타났으며, 16S rDNA sequencing을 행한 결과 14.92 bp.의 염기서열을 결정하고, referance와 비교하여 % simmilarity를 구한 결과 이 균주는 Bacillus속에 속하는 세균으로 판명되었으며, 새로운 종이라 판단하여 Bacillus alkalophilshaggy JY-827균이라 명명하였다. The study was performed to investigate the excellent microbial anticaries substance which is more effective than the chlorohexidine in the dental caries treatment. For the screening of alkaliphilic microorganism, more than 1200 bacterial strains were isolated from sea soil sample. A typical strain which produced the most excellent antimicrobial substance was selected. The strain was identified novel alkalophillic Bacillus sp. through the results of morphological, biochemical and chemotaxonomical characteristics and 16S rDNA sequencing and designated as Bacillus alkalophilshaggy JY-827.

전국 보건(지)소 치과위생사 업무실태 및 의식구조에 관한 조사연구

전주연,이성림 원광보건전문대 1995 원광보건전문대학논문집 Vol.18 No.-