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      • Synthesis of Maltoheptaose Fatty Ester by Yeast Lipase and Their Emulsion Functions

        Phu Cuong Nguyen,Thi My Tuyen Nguyen,Chang-KyuLee,Il-NamOh,Min-Jee Yoo,Kyu-BeenPark,Jae-HanKim,Hyun-JooAn,Jong-TaePark 한국당과학회 2018 한국당과학회 학술대회 Vol.2018 No.07

        Enzymatic syntheses of sugar fatty acid (FA) esters are important because of their wide range of applications in the food, cosmetic, and pharmaceutical industries. These molecules are commonly synthesized from F As and mono-, or di-saccharides by chemical or enzymatic esterification. Previously, sugar esters were produced using oligosaccharide, and interfacial tension values and inhibitory effects towards tumor cell lines were improved when higher DP saccharides were used. In this study, the direct esterification of maltoheptaose (G7) with vanous length of F As was investigated by the catalytic action of Candida antartica lipase B. Among the F As palmitic acid (PA) was the best substrate for the G7 ester formation. Optimal reaction conditions including solvent composition and molar ratio of G 7: P A were established for the G7-PA. The conversion yield and sugar ester structure were analyzed using various instrumental analyses. The results showed that G7-PA monoester was successfully synthesized with a molar ratio of I :20 (G7: PA) in I 0% DMSO of t-butanol at 60°C, and the conversion yield was over than 21%. The position of ester bond formation was identified that PA was attached to C-6 of the reducing end glucosyl moiety. The melting temperature of G7- PA monoester was reached at 56 °C, lower than that of pure G7 and PA. In addition, the G7- p A esters and commercial sucrose esters were compared as emulsifiers. Both type of sugar esters prevented coalescence during 7 days and showed good emulsifying properties in 0/W emulsions since the droplets diameter did not have significant change during the storage.

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        꽃노랑총채벌레와 대만총채벌레의 내한성과 월동처 비교 연구

        김철영,최두열,팔구니칸,엠디따핌호사인흐리틱,홍주안,김용균,Chulyoung, Kim,Du-yeol, Choi,Falguni, Khan,Md Tafim Hossain, Hrithik,Jooan, Hong,Yonggyun, Kim 한국응용곤충학회 2022 한국응용곤충학회지 Vol.61 No.3

        Two dominant thrips in hot pepper (Capsicum annuum) cultivating in greenhouses are Frankliniella occidentalis and F. intonsa in Korea. This study investigated their overwintering physiology. These two thrips were freeze-susceptible and suppressed the body freezing temperature by lowering supercooling point (SCP) down to -15~-27℃. However, these SCPs varied among species and developmental stages. SCPs of F. occidentalis were -25.7±0.5℃ for adults, -17.2±0.3℃ for pupae, and -15.0±0.4℃ for larvae. SCPs of F. intonsa were -24.0±1.0℃ for adults, -27.0±0.5℃ for pupae, -17.2±0.8℃ for larvae. Cold injuries of both species occurred at low temperature treatments above SCPs. Thrips mortality increased as the treatment temperature decreased and its exposure period increased. F. occidentalis exhibited higher cold tolerance than F. intonsa. In both species, adults were more cold-tolerant than larvae. Two thrips species exhibited a rapid cold hardening because a pre-exposure to 0℃ for 2 h significantly enhanced the cold tolerance to a lethal cold temperature treatment at -10℃ for 2 h. In addition, a sequential exposure of the thrips to decreasing temperatures made them to be acclimated to low temperatures. To investigate the overwintering sites of the two species, winter monitoring of the thrips was performed at the greenhouses. During winter season (November~February), adults of the two species were not captured in outside of the greenhouses. However, F. occidentalis adults were captured to the traps and observed in weeds within the greenhouses. F. occidentalis adults were also emerged from soil samples obtained from the greenhouses during the winter season. F. intonsa adults did not come out from the soil samples at November and December, but emerged from the soil samples obtained after January. To determine the adult emergence due to diapause development, two thrips species were reared under different photoperiods. Adult development occurred in all photoperiod treatments in F. occidentalis, but did not in F. intonsa especially under short periods. Tomato spotted wilt virus, which is transmitted by these two species, was detected in the weeds infested by the thrips during the winter season. These results suggest that F. occidentalis develops on weeds in the greenhouses while F. intonsa undergoes a diapause in the soil during winter.

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