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Yonggyun Kim,Yerim Son 한국응용곤충학회 2006 Journal of Asia-Pacific Entomology Vol.9 No.1
solitary endoparsitoid,Cotesia plutellae,has ben regarded as a major biological regulatorto manipulate field population of diamondback moth(DBM),Plutella xylostella. It parasitizes DBM andalters its physiology into a favorable condition forthe parasitoid evelopment. This research has beenfocused on the physiological changes in terms ofinternal morphology and biochemical changes of theparasitized DBM. The parasitized DBM exhibitedsignificantly hypotrophied structures in Malpighianapparent change in the digestive organ structure. Theparasitoid represented almost 70% of total bodyweight of the parasitized DBM. This estimate waswell corresponded to the measurements of the majornutrient amounts in the parasitized DBM. This studyclearly indicates that the major nutrients obtained bythe parasitoid DBM are exploited by the koinobiontparasitoid for its own development.Key words Cotesia plutellae, Internal morphology,Nutrient, Parasitism,Plutella xylostella
Yonggyun Kim,Samyeol Seo,Yerim Son 한국응용곤충학회 2012 한국응용곤충학회 학술대회논문집 Vol.2012 No.05
Sound treatments have been considered as a non-chemical insect pest control technique. Different frequency and intensity sounds were applied to immune and adult stages to screen any stress sounds to alter physiological processes. At 95 dB, 5,000 Hz and 30,000 Hz were selected to be stress sounds in audible and inaudible sound ranges, respectively. Both stress sounds significantly inhibited larval and pupal development. In biochemical analyses, lipid and sugar levels in plasma signigicantly increased in response to the stress sound treatments. Moreover, a digestive phospholipase A2 enzyme activity in midgut was significantly reduced. In adult stage, ultrasound treatment significantly inhibited mating behavior, which resulted in a reduced fecundity. These stress sounds altered gene expressions of stress-related genes, such as heat-shock proteins and apolipophorin III. This study suggests that extreme sounds play a role in physiological stress factors in S. exigua by altering developmental and reproductive processes.
Revision of CpBV polydnaviral viral genome composition and expression profile
Yonggyun Kim 한국응용곤충학회 2016 한국응용곤충학회 학술대회논문집 Vol.2016 No.04
Cotesia plutellae bracovirus (CpBV) is a bracoviral polydnavirus and a parasitic factor of an endoparasitioid wasp, C. plutellae, which parasitizes the diamondback moth (DBM) larvae. CpBV genome was reported to consist of 35 circular segments and encode 157 predicted open reading frames (ORFs). This study revisited and re-annotated the CpBV genome. Total ORFs consist of 15 groups of known gene or gene families as well as hypothetical (HP) group. All 74 HP genes are matched to other PDV genes. RNASeq analysis indicates that 147 genes out of 157 predicted ORFs are expressed in the parasitized DBM at the first day or 7 days after parasitization. At the early parasitic stage, CpBV genes annotated as ELP, BEN, E94K, DUFB, PTP, and HP are highly expressed compared to late parasitic stage. Interestingly, highly expressed genes at the late parasitic stages are HPs.
Yonggyun Kim,Ahmed M. A. Ibrahim,Sungchae Jung,Min Kwoen 한국응용곤충학회 2006 Journal of Asia-Pacific Entomology Vol.9 No.3
Two closely-related endoparasitoids of Cotesia plutellae and C. glomerata parasitize the diamondback moth, Plutella xylostella. The parasitized hosts by either parasitoid species exhibited the extended larval period and died without further metamorphosis to pupal stage. However, two parasitoid species exhibited significantly different parasitic capacity and developmental rate, in which C. plutellae showed higher parasitism and faster development in the parasitized P. xylostella. To discriminate these two similar species, morphological and molecular differences were analyzed. Three dichotomous morphological characters including antennal flagellum, hind-leg femur, and terminal abdominal terga were determined. Based on the presumptive polydnaviral particles found in the ovarian calyx of C. glomerata, three genes similar to C. plutellae bracoviral genes were cloned in the C. glomerata genome and compared in their cDNA and the deduced amino acid sequences. Several polymorphic sites were detected to be applicable to design molecular markers to discriminate these two species.
Yonggyun Park,Daesu Han,Yongsug Suh,Wooyoung Choi 전력전자학회 2013 JOURNAL OF POWER ELECTRONICS Vol.13 No.6
This paper investigates control algorithms for a doubly fed induction generator with a back-to-back three-level neutral-point clamped voltage source converter in medium voltage wind power systems under unbalanced grid conditions. Three different control algorithms to compensate for unbalanced conditions have been investigated with respect to four performance factors; fault ride-through capability, instantaneous active power pulsation, harmonic distortions and torque pulsation. The control algorithm having a zero amplitude of torque ripple shows the most cost-effective performance concerning torque pulsation. The least active power pulsation is produced by the control algorithm that nullifies the oscillating component of the instantaneous stator active and reactive powers. A combination of these two control algorithms depending on the operating requirements and the depth of the grid unbalance presents the most optimized performance factors under generalized unbalanced operating conditions leading to high performance DFIG wind turbine systems.
Yonggyun Kim,Joonik Lee,Sungyoung Kang,Sangchan Han Korean Society of Applied Entomology 1998 Journal of Asia-Pacific Entomology Vol.1 No.1
The susceptibility of Spodoptera exigua(H$\ddot{u}$bner) to bifenthrin and chlorpyrifos-methyl in relation to larval development was investigated. Increased tolerances to these insecticides were associated with increasing larval instars. The insecticide tolerance increased linealy with larval body weights in bifenthrin but did exponentially in chlorpyrifos-methyl. Esterase(EST), acetyl-cholinesterase(AChE), and glutathione S-transferase(GST) of different larval ages were analyzed to elucidate variation of insecticide susceptibilities according to larval development within a population. Total EST activity increased linearly with body weights though the specific activities were not varied among ages. Both total and specific activity changes of GST, however, surpassed the rates of body weight gains as the larvae developed. AChE activities was due to the developmental change of its catalytic function. The fifth instar larvae had the lowest catalytic capacity: the highest $K_{m}$ (187.39$\mu\textrm{M}$) and the lowest $V_{max}$ (0.58nM/min/$\mu\textrm{g}$). Therefore, different insecticide tolerance of S. exigua according to larval ages can be explained by both enhanced detoxification enzymes and altered AchE.