Differentiation of human labia minora dermis-derived fibroblasts into insulin-producing cells

Kim, Bona,Yoon, Byung Sun,Moon, Jai-Hee,Kim, Jonggun,Jun, Eun Kyoung,Lee, Jung Han,Kim, Jun Sung,Baik, Cheong Soon,Kim, Aeree,Whang, Kwang Youn,You, Seungkwon Korean Society for Biochemistry and Molecular Bion 2012 Experimental and molecular medicine Vol.44 No.1

Recent evidence has suggested that human skin fibroblasts may represent a novel source of therapeutic stem cells. In this study, we report a 3-stage method to induce the differentiation of skin fibroblasts into insulin-producing cells (IPCs). In stage 1, we establish the isolation, expansion and characterization of mesenchymal stem cells from human labia minora dermis-derived fibroblasts (hLMDFs) (stage 1: MSC expansion). hLMDFs express the typical mesenchymal stem cell marker proteins and can differentiate into adipocytes, osteoblasts, chondrocytes or muscle cells. In stage 2, DMEM/F12 serum-free medium with ITS mix (insulin, transferrin, and selenite) is used to induce differentiation of hLMDFs into endoderm-like cells, as determined by the expression of the endoderm markers Sox17, Foxa2, and PDX1 (stage 2: mesenchymal-endoderm transition). In stage 3, cells in the mesenchymal-endoderm transition stage are treated with nicotinamide in order to further differentiate into self-assembled, 3-dimensional islet cell-like clusters that express multiple genes related to pancreatic ${\beta}$-cell development and function (stage 3: IPC). We also found that the transplantation of IPCs can normalize blood glucose levels and rescue glucose homeostasis in streptozotocin-induced diabetic mice. These results indicate that hLMDFs have the capacity to differentiate into functionally competent IPCs and represent a potential cell-based treatment for diabetes mellitus.

Effect of Support Structures on the Deformation of AlSi10Mg Aircraft Parts Made Using DMLS

Jonggun Kim,Jae Hyun Park,Sunghwa Jang,Hyeonghwan Jeong,TaeGyu Kim,Hyung Giun Kim 한국정밀공학회 2023 International Journal of Precision Engineering and Vol.24 No.5

Additive manufacturing (AM or 3D printing) technology creates the same shape to 3D CAD data through an additive process rather than using a conventional manufacturing method. This has made it possible to surpass the limitations of existing processing methods. Because of the many advantages of AM technology, many studies have been conducted to apply AM technology for the manufacture of aircraft structures. In this work, in particular, the influence of build parameters, mechanical properties, and post-processing were reviewed with the aim of determining the effect of support among the build parameters. The tip-cap of an aircraft wing was fabricated using AlSi10Mg powder, and the deformation during post-processing was reviewed according to three types of support. Mechanical properties were verified through specimen testing, and parts were fabricated and post-processing applied, under the same conditions as for the specimens. Based on the existing CAD model, it was discovered that deformation occurred during each type of post-processing and the influence of support on the deformation was reviewed.

항공기 부품개발을 위한 형상관리 기법 소개

김종건(Jonggun Kim),김동석(Dong Seok Kim),신대영(Dae Young Shin),김승철(Seung Chul Kim) 한국항공우주학회 2018 한국항공우주학회 학술발표회 논문집 Vol.2018 No.4

Applying Focused and Radial Shock Wave for Calcific Tendinitis of the Shoulder : Randomized Controlled Study

Jonggun Kim,Changmin Oh,John Yoo,Jong Eun Yim 물리치료재활과학회 2022 Physical therapy rehabilitation science Vol.11 No.3

Objective: Extracorporeal shock wave therapy (ESWT) is a nonsurgical treatment alternative to surgery for various musculoskeletal diseases that have traditionally been difficult to treat conservatively, including calcific tendinitis, tennis elbow, and plantar fasciitis.This study evaluated the effect of focused and radial shock wave therapy for calcific tendinitis of the shoulder. Design: Randomized controlled studyMethods: Forty participants with calcific tendinitis were randomized into focused shock wave therapy (FSWT, n=20) and radial shock wave therapy (RSWT, n=20) groups. Patients were examined before and one week after treatment. Pain intensity was subjectively assessed using the visual analogue scale and function was assessed using theConstant-Murley score (CMS) and range of motion (ROM). Results: The results showed a significant decrease in pain and significant increase in shoulder mobility and function in both groups. However, FSWT was significantly more effective than RSWT, based on CMS and ROM assessment. Conclusions: Although it is possible to raise the energy intensity of RSWT to increase the depth at which the energy becomes dispersed, higher energy intensity is associated with a greater risk of severe neurovascular damage, and that high-intensity stimulation can cause adverse effects such as pain and petechiae . Therefore, FSWT is considered to be a safe and effective method for treating tendinous lesions while minimizing adverse effects. In conclusion, both FSWT and RSWT can reduce pain and increase mobility and function. FSWT can be considered as an alternative for calcific tendinitis of the shoulder.

직접유출량 모의를 위한 ArcGIS 기반의 모형 개발 및 개선

김종건 ( Kim Jonggun ),임경재 ( Lim Kyoung Jae ),( Engel Bernie ),차상선 ( Cha Sang Sun ),박찬기 ( Park Chan-gi ),박윤식 ( Park Youn Shik ) 한국농공학회 2018 한국농공학회논문집 Vol.60 No.6

The Long-Term Hydrologic Impact Assessment (L-THIA) model is a quick and straightforward analysis tool to estimate direct runoff and nonpoint source pollution. L-THIA was originally implemented as a spreadsheet application. GIS-based versions of L-THIA have been developed in ArcView 3 and upgraded to ArcGIS 9. However, a major upgrade was required for L-THIA to operate in the current version of ArcGIS and to provide more options in runoff and NPS estimation. An updated L-THIA interfaced with ArcGIS 10.0 and 10.1 has been developed in the study as an ArcGIS Desktop Tool. The model provides a user-friendly interface, easy access to the model parameters, and an automated watershed delineation process. The model allows use of precipitation data from multiple gauge locations for the watershed when a watershed is large enough to have more than one precipitation gauge station. The model estimated annual direct runoff well for our study area compared to separated direct runoff in the calibration and validation periods of ten and nine years. The ArcL-THIA, with a user-friendly interface and enhanced functions, is expected to be a decision support model requiring less effort for GIS processes or to be a useful educational hydrology model.

농촌비점오염관리를 위한 농업배출수 수량 및 수질관리 방안

김종건 ( Jonggun Kim ),양동석 ( Dongseok Yang ),최완민 ( Wanmin Choi ),최선화 ( Seonhwa Choi ),임경재 ( Kyoung Jae Lim ) 한국농공학회 2020 한국농공학회 학술대회초록집 Vol.2020 No.-

최근 물관리기본법 시행에 따른 통합물관리체제로 전환되면서 농업용수에 대한 가치가 재평가되고 있고 농업용수의 효율적 활용을 위한 다양한 연구가 진행되고 있다. 2019년 기준 우리나라 수자원 총량 대비 총 이용량은 약 28%(372억 ㎥)를 차지하고 있으며, 그 중 농업용수는 총 이용량의 약 61%(152억 ㎥)를 차지하고 있다. 또한 농업용수 이용량의 약 80%가 논에서 사용되고 있다. 우리나라에서는 논에서 사용되고 있는 용수가 상대적으로 많은 부분을 차지하고 있으며, 그에 따라 사용되고 배출되는 수량은 하류 하천의 수량 및 수질, 수생태에 영향을 미치고 있다. 현재 농업용수 배출수의 수량 및 수질에 대해 정량화할 수 있는 정보가 부족하여 농업용수의 효율적인 이용을 저해하고 있다. 농업용수 배출수는 하류 하천 수질에 영향을 미쳐 농촌지역에서의 주요 비점오염원으로 인식되고 있다. 따라서 통합물관리체제에서 농업용수 배출수에 대한 수량 및 수질 관리가 필요한 시점이며, 농업용수의 효율성, 안전성, 안정성을 확보하기 위해 정밀한 농업배출수 산정 및 양질의 농업용수 확보를 위한 다양한 노력이 필요하다. 우선 농업배출수의 수량적인 측면에서 농업용수 수원공에서의 정확한 공급량 산정 기술이 필요하며, 이를 기반으로 농경지 공급 후 하류 하천으로 배출되는 회귀수의 정량적 산정 방법이 필요하다. 이를 통해 농업용수가 하류 하천의 환경유지용수로서의 가치 평가가 가능할 것이다. 농업배출수의 수량확보를 위해서는 용수 재이용, SRI 기법 적용, 효율적 용수공급 등 용수의 수요를 줄이고 환경유지용수를 확보하여야 할 것이다. 농업용수 배출수의 수질측면에서는 농업용수 수원공을 대상으로 상·하류 유역단위모니터링 연구를 통해 기초자료를 수집하고, 이를 통해 농업용수의 맑은 물을 확보할 수 있는 통합관리 체계 구축 방안이 마련되어야 할 것이다. 기존의 단순 수질개선대책 중심에서 수량·수질·수생태 통합물관리 측면 유황별 또는 영농기간별 특성을 고려한 종합적 관리 체계 구축, 그리고 이를 통한 실질적인 농업용수 관리 대책 수립이 필요할 것이다.

수문학적 공간 연결성을 고려한 토양물환경 해석 및 토양수문정보 구축 방안 연구

김종건 ( Kim Jonggun ) 한국농공학회 2018 한국농공학회 학술대회초록집 Vol.2018 No.-

Subsurface flow is an important component to understand soil water environment in the unsaturated zone and plays a significant role in the water and energy balance through affecting various hydrological processes. Land surface models (LSMs) have been developed and extended during the past decades with various enhanced processes to understand and quantify the complex interaction between atmosphere and land surface systems. However, there are still critical deficiencies (e.g., simplified processes and parameterization) remaining in simulating land surface hydrology for land surface modeling. Thus, this study focuses on understanding soil water environment based on hydrologic spatial connectivity and improving land surface processes and parameterization in land surface modeling in the unsaturated zone. The physically-based hydrologic connectivity concept was proposed to characterize the subsurface flow variability based on spatially distributed patterns of wetness condition or physical controls (e.g., soil type, vegetation, topography). Hydrologic connectivity is an important concept for understanding local processes in the context of catchment hydrology and defining flow path continuity in surface and subsurface flows. This approach was applied in land surface modeling and tested in various hydro-climate regions and spatial scales showing significant improvement of modeling predictability. Based on the knowledge and experience, the proposed concept will be useful to improve the hydrological model performance and better understand soil water environment in the unsaturated zone. Furthermore, this study proposed an enhanced soil hydrological monitoring approach based on ICT technologies. Recently, advanced ICT technologies including low-cost sensors based sensing technology have been applied to various fields. Thus, this study suggests efficient monitoring technology using the advanced ICT technology.

머신러닝 기법을 활용한 유황별 LOADEST 모형의 적정 회귀식 선정 연구: 낙동강 수계를 중심으로

김종건 ( Jonggun Kim ),박윤식 ( Youn Shik Park ),이서로 ( Seoro Lee ),신용철 ( Yongchul Shin ),임경재 ( Kyoung Jae Lim ),김기성 ( Ki-sung Kim ) 한국농공학회 2017 한국농공학회 학술대회초록집 Vol.2017 No.-

This study is to determine the coefficients of regression equations and to select the optimal regression equation in the LOADEST model after classifying the whole study period into 5 flow conditions for 16 watersheds located in the Nakdonggang waterbody. The optimized coefficients of regression equations were derived using the gradient descent method as a learning method in Tensorflow which is the engine of machine-learning method. In South Korea, the variability of streamflow is relatively high, and rainfall is concentrated in summer that can significantly affect the characteristic analysis of pollutant loads. Thus, unlike the previous application of the LOADEST model(adjusting whole study period), the study period was classified into 5 flow conditions to estimate the optimized coefficients and regression equations in the LOADEST model. As shown in the results, the equation #9 which has 7 coefficients related to flow and seasonal characteristics was selected for each flow condition in the study watersheds. When compared the simulated load(SS) to observed load, the simulation showed a similar pattern to the observation for the high flow condition due to the flow parameters related to precipitation directly. On the other hand, although the simulated load showed a similar pattern to observation in several watersheds, most of study watersheds showed large differences for the low flow conditions. This is because the pollutant load during low flow conditions might be significantly affected by baseflow or point-source pollutant load. Thus, based on the results of this study, it can be found that to estimate the continuous pollutant load properly the regression equations need to be determined with proper coefficients based on various flow conditions in watersheds. Furthermore, the machine-learning method can be useful to estimate the coefficients of regression equations in the LOADEST model.

SWMM 모형을 이용한 홍수시 바이모달 트램 운행 노선에 대한 침수 면적 산정 모듈 개발

김종건(Jonggun Kim),박영곤(Youngkon Park),윤희택(Heetaek Yoon),박윤식(Younshik Park),장원석(Wonseok Jang),유동선(Dongseon Yoo),임경재(Kyoung Jae Lim) 한국철도학회 2008 한국철도학회 학술발표대회논문집 Vol.- No.-

Urban flooding with surcharges in sewer system was investigated because of unexpected torrential storm events these days, causing significant amounts of human and economic damages. Although there are limitations in forecasting and preventing natural disasters, integrated urban flooding management system using the SWMM engine and Web technology will be an effective tool in securing safety in operating Bi-modal transportation system. In addition, the integrated urban flooding management system can be linked with general and transportation-related disaster management system in the future. In this study, With simulated values by the SWMM, which is a core engine of the Bi-modal disaster management system, flash flooding area estimation module was developed. Thus, the SWMM system codes were modified and new module was developed and integrated with the existing SWMM interface using the Delphi programming language. The flash flooding area estimation module is fully integrated with the SWMM interface, thus the area is estimated on-the-fly inside the system.

Differentiation of human labia minora dermis-derived fibroblasts into insulin-producing cells

Bona Kim,Byung Sun Yoon,Jai-Hee Moon,Jonggun Kim,Eun Kyoung Jun,Jung Han Lee,Jun Sung Kim,Cheong Soon Baik,김애리,황광연,유승권 생화학분자생물학회 2012 Experimental and molecular medicine Vol.44 No.1

Recent evidence has suggested that human skin fibroblasts may represent a novel source of therapeutic stem cells. In this study, we report a 3-stage method to induce the differentiation of skin fibroblasts into insulin-producing cells (IPCs). In stage 1, we establish the isolation, expansion and characterization of mesenchymal stem cells from human labia minora dermis-derived fibroblasts (hLMDFs) (stage 1: MSC expansion). hLMDFs express the typical mesenchymal stem cell marker proteins and can differentiate into adipocytes,osteoblasts, chondrocytes or muscle cells. In stage 2, DMEM/F12 serum-free medium with ITS mix (insulin, transferrin, and selenite) is used to induce differentiation of hLMDFs into endoderm-like cells, as determined by the expression of the endoderm markers Sox17, Foxa2, and PDX1 (stage 2: mesenchymal-endoderm transition). In stage 3, cells in the mesenchymal-endoderm transition stage are treated with nicotinamide in order to further differentiate into self-assembled,3-dimensional islet cell-like clusters that express multiple genes related to pancreatic β-cell development and function (stage 3: IPC). We also found that the transplantation of IPCs can normalize blood glucose levels and rescue glucose homeostasis in streptozotocin-induced diabetic mice. These results indicate that hLMDFs have the capacity to differentiate into functionally competent IPCs and represent a potential cell-based treatment for diabetes mellitus.