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유승권,Yu, Seung-Gwon 한국기술사회 2007 技術士 Vol.40 No.6
The government announced to launch tours to the Mt.Baekdu that use a direct air route through the 2007 South-North Korean leader joint declaration. The Mt.Baekdu tourism is large symbolism to the Korean people. Government and Hyundai Asan speak that propel Mt.Baekdu tourism using existent Samjiyeon airport in early time. But, air traffic and airport operation must consider safety preferentially. Present condition investigation and facility improvement of the Samjlyeon airport should be enforced first. And need facility expansion by increased demand hereafter. Compose South-North Air traffic network system to this opportunity and basis research and phase investment may have to be propelled.
Cellular characteristics of primary and immortal canine embryonic fibroblast cells
유승권,문재희,김태경,김성찬,김재우,윤두학,곽성욱,홍기창,최윤재,김형기 생화학분자생물학회 2004 Experimental and molecular medicine Vol.36 No.4
Using normal canine embryonic fibroblasts (CaEF) that were shown to be senescent at passages 7th-9th, we established two spontaneously immortalized CaEF cell lines (designated CGFR-Ca-1 and -2) from normal senescent CaEF cells, and an immortal CaEF cell line by exogenous introduction of a catalytic telomerase subunit (designated CGFR-Ca-3). Immortal CGFRCa- 1, -2 and -3 cell lines grew faster than primary CaEF counterpart in the presence of either 0.1% or 10% FBS. Cell cycle analysis demonstrated that all three immortal CaEF cell lines contained a significantly high proportion of S-phase cells compared to primary CaEF cells. CGFR-Ca-1 and -3 cell lines showed a loss of p53 mRNA and protein expression leading to inactivation of p53 regulatory function, while the CGFR-Ca-2 cell line was found to have the inactive mutant p53. Unlike the CGFR-Ca-3 cell line that down-regulated p16INK4a mRNA due to its promoter methylation but had an intact p16INK4a regulatory function, CGFR-C a-1 and -2 cell lines expressed p16INK4a mRNA but had a functionally inactive p16INK4a regulatory pathway as judged by the lack of obvious differences in cell growth and phenotype when reconstituted with wild-type p16INK4a. All CGFR-C a-1, -2 and -3 cell lines were shown to be untransformed but immortal as determined by anchorage-dependent assay, w hile these cell lines were fully transformed when overexpressed oncogenic H-rasG12V. Taken together, similar to the nature of murine embryo fibroblasts, the present study suggests that normal primary CaEF cells have relatively short in vitro lifespans and should be spontaneously immortalized at high frequency.