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Beneficial Effects of Cynaroside on Cisplatin-Induced Kidney Injury In Vitro and In Vivo
Jong-Hyun Nho,Ho-Kyung Jung,Mu-Jin Lee,Ji-Hun Jang,Mi-Ok Sim,Da-Eun Jeong,Hyun-Woo Cho,Jong-Choon Kim 한국독성학회 2018 Toxicological Research Vol.34 No.2
Anti-cancer drugs such as cisplatin and doxorubicin are effectively used more than radiotherapy. Cisplatin is a chemotherapeutic drug, used for treatment of various forms of cancer. However, it has side effects such as ototoxicity and nephrotoxicity. Cisplatin-induced nephrotoxicity increases tubular damage and renal dysfunction. Consequently, we investigated the beneficial effect of cynaroside on cisplatin-induced kidney injury using HK-2 cell (human proximal tubule cell line) and an animal model. Results indicated that 10 μM cynaroside diminished cisplatin-induced apoptosis, mitochondrial dysfunction and caspase-3 activation, cisplatin-induced upregulation of caspase-3/MST-1 pathway decreased by treatment of cynaroside in HK-2 cells. To confirm the effect of cynaroside on cisplatin-induced kidney injury in vivo, we used cisplatin exposure animal model (20 mg/kg, balb/c mice, i.p., once a day for 3 days). Renal dysfunction, tubular damage and neutrophilia induced by cisplatin injection were decreased by cynaroside (10 mg/kg, i.p., once a day for 3 days). Results indicated that cynaroside decreased cisplatin-induced kidney injury in vitro and in vivo, and it could be used for improving cisplatin-induced side effects. However, further experiments are required regarding toxicity by high dose cynaroside and caspase-3/MST-1-linked signal transduction in the animal model.
Beneficial Effects of Cynaroside on Cisplatin-Induced Kidney Injury In Vitro and In Vivo
Nho, Jong-Hyun,Jung, Ho-Kyung,Lee, Mu-Jin,Jang, Ji-Hun,Sim, Mi-Ok,Jeong, Da-Eun,Cho, Hyun-Woo,Kim, Jong-Choon Korean Society of ToxicologyKorea Environmental Mu 2018 Toxicological Research Vol.34 No.2
Anti-cancer drugs such as cisplatin and doxorubicin are effectively used more than radiotherapy. Cisplatin is a chemotherapeutic drug, used for treatment of various forms of cancer. However, it has side effects such as ototoxicity and nephrotoxicity. Cisplatin-induced nephrotoxicity increases tubular damage and renal dysfunction. Consequently, we investigated the beneficial effect of cynaroside on cisplatin-induced kidney injury using HK-2 cell (human proximal tubule cell line) and an animal model. Results indicated that $10{\mu}M$ cynaroside diminished cisplatin-induced apoptosis, mitochondrial dysfunction and caspase-3 activation, cisplatin-induced upregulation of caspase-3/MST-1 pathway decreased by treatment of cynaroside in HK-2 cells. To confirm the effect of cynaroside on cisplatin-induced kidney injury in vivo, we used cisplatin exposure animal model (20 mg/kg, balb/c mice, i.p., once a day for 3 days). Renal dysfunction, tubular damage and neutrophilia induced by cisplatin injection were decreased by cynaroside (10 mg/kg, i.p., once a day for 3 days). Results indicated that cynaroside decreased cisplatin-induced kidney injury in vitro and in vivo, and it could be used for improving cisplatin-induced side effects. However, further experiments are required regarding toxicity by high dose cynaroside and caspase-3/MST-1-linked signal transduction in the animal model.
엉겅퀴 뿌리 물 추출물의 류마티스 관절염 동물 모델에 대한 개선 효과
노종현(Jong Hyun Nho),이현주(Hyeun Joo Lee),이에나(E Na Lee),우경완(Kyeong Wan Woo),장지훈(Ji Hun Jang),김선라(Sun Ra Kim),조현우(Hyun Woo Cho),노세응(Se Eung Noh),정호경(Ho Kyung Jung) 한국약용작물학회 2020 한국약용작물학회지 Vol.28 No.6
Background: The roots of Cirsium japonicum var. ussuriense (RCJ) have been used as traditional medicine in Korea for hematuria and hematemesis. These extracts exert anti-oxidative and anti-inflammatory effects by scavenging for free radical and regulating the inflammatory response. However, the effect of RCJ on rheumatoid arthritis (RA) has not been elucidated. Thus, we evaluated the water extract of RCJ (WRCJ) using type II collagen-induced RA models. Methods and Results: RA was induced by immunization with type II collagen. All experimental materials were orally administered daily for three weeks. The positive control group was administered with 0.2 ㎎/㎏ methotrexate (n = 7), while the experimental group was administered with WRCJ (100 or 500 ㎎/㎏, n = 7). Serum levels of TNF-alpha, Interleukin 6 (IL-6), and type II collagen IgG (CII) were measured using ELISA. Administration of 500 ㎎/㎏ WRCJ decreased the levels of TNF-alpha, IL-6, and CII. Moreover, WRCJ treatment diminished swelling of hind legs and infiltration of inflammatory cells in RA models’ synovial membrane. Conclusions: These results indicate that WRCJ could improve RA, reduce inflammatory indicators and synovial inflammation. However, further experiments are required to determine how WRCJ can influence the signal transduction pathway in RA.
노종현(Jong Hyun Nho),장지훈(Ji Hun Jang),이무진(Mu Jin Lee),양버들(Beodul Yang),우경완(Kyeong Wan Woo),이현주(Hyun Joo Lee),김아현(A Hyeon Kim),심미옥(Mi Ok Sim),조현우(Hyun Woo Cho),정호경(Ho Kyung Jung) 한국약용작물학회 2019 한국약용작물학회지 Vol.27 No.3
Background: Galgeun-tang used in traditional Korean medicine, is a mixture of the medicinal plants Cinnamomi Ramulus, Ephedrae Herba and Puerariae Radix, and has been prescribed for the treatment of various ailments, including fever. Although the use of traditional medicinal herbs to treat diseases has recently increased, their safety and toxicity profiles incompletely elucidated. Thus, we evaluated Galgeun-tang’s toxicity in male and female Sprague-Dawley rats. Methods and Results: Galgeun-tang (1,000, 2,000 and 4,000 ㎎/㎏) was orally administered to rats for 13 weeks, and then, they were maintained for 4 weeks without administration (recovery period). Their clinical signs, and hematological and urinary properties, were monitored. The results showed that Galgeun-tang administeration slightly increased serum creatinine, urea nitrogen and, aspartate aminotransferase levels. Additionally, 2,000 and 4,000 ㎎/㎏ Galgeun-tang significantly increased urinary bilirubn and protein levels of male and female rats, which were restored during the recovery period. Conclusions: The no-observed-adverse-effect level of orally administered Galgeun-tang was 4,000 ㎎/㎏ in both female and male rats, and no target organs were identified. In addition, 400 ㎎/㎏ was found to be the no-observed-effect level for toxicity under the study conditions.
Subchronic toxicity evaluation of ethanol extract of <i>Cassia tora</i> L. seeds in rats
Lee, Mu-Jin,Nho, Jong-Hyun,Yang, Beo-Deul,Park, Ho,Lee, Hyun-Joo,Lee, Ki-Ho,Jang, Ji-Hun,Jung, Ho-Kyung,Kim, Sun-Ra,Cho, Hyun-Woo,Park, Hae-Sung,Lim, Je-Oh,Kim, Jong-Choon Elsevier 2019 Regulatory toxicology and pharmacology Vol.109 No.-
<P><B>Abstract</B></P> <P> <I>Cassia tora</I> Linn. is an annual or perennial plant of the Fabaceae/Leguminosae family. It is used in traditional medicine for various biological activities including anti-constipation, anti-inflammatory, visual acuity, and hepato-protective activities. The present study was carried out to investigate the potential toxicity of <I>C. tora</I> L. seed ethanol extract (CTSEE) following a 13-week repeated oral administration to Sprague-Dawley rats. CTSEE was administered orally to male and female rats for 13 weeks at 0 (control), 500, 1000, and 2000 mg/kg/day (<I>n</I> = 10, for male and female rats for each dose). Additional recovery groups from the control group and high dose group were observed for a 4-week recovery period. At the end of the treatment and recovery periods, animals were sacrificed, and their organs were weighed and blood samples collected. There were no treatment-related adverse effects in clinical signs, body weight, food consumption, estrous cycle, sperm parameters, urinalysis, hematology, serum biochemistry, necropsy findings, organ weight, and histopathology at any doses tested. Under the present experimental conditions, the no-observed-adverse-effect level of the CTSEE was >2000 mg/kg/day in both genders, and no target organs were identified.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Subchronic toxicity of <I>Cassia tora</I> L. seed ethanol extract (CTSEE) were investigated. </LI> <LI> No significant adverse effects were observed in the repeated dose toxicity study. </LI> <LI> The oral NOAEL of CTSEE was >2000 mg/kg/day in rats of both genders. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>
( Ji-hun Jang ),( Ki-ho Lee ),( Jong-hyun Nho ),( Hyun-joo Lee ),( Beo-dul Yang ),( Ho Park ),( Hyun-woo Cho ),( Byeong-kwan An ),( Sun-ra Kim ),( Ju-hyun Yong ),( Ro-dong Park ),( Ho-kyung Jung ) 한국응용생명화학회(구 한국농화학회) 2020 Journal of Applied Biological Chemistry (J. Appl. Vol.63 No.4
We previously reported the potential of Senna tora L. seeds fermented by Lactobacillus casei (FSL) as a laxative agent in a loperamide-induced constipation rat model. Here, we examine the mechanism of action of FSL and its bioactive compound, revealed herein, on loperamide-induced constipation Sprague Dawley rat model. We identified the compound aurantio-obtusin (AO) using HPLC quantitative analysis. Rats were randomly assigned to six experimental groups (eight rats each)-normal and constipated groups (loperamide, FSL [100, 300, 500 mg/kg], and AO [1 mg/kg]). The FSL and AO-treated group showed an increase in the frequency, amount, and water content of feces in the constipated rat. Moreover, FSL and AO increased the intestinal transit speed in the constipated rat. Histological analysis revealed that FSL and AO recovered the intestinal mucus, the number of goblet cells, as well as thickness of the mucosa layer and muscle. Furthermore, the protein levels of the muscarinic acetylcholine receptor M3, which is involved in intestine contraction, were recovered in the FSL and AO-treated group. Its downstream signaling pathway (p-protein kinase C) was recovered by FSL and AO treatment. In conclusion, fermentation of S. tora L. seeds increases AO, which improves intestinal function, indicating that FSL is effective for treating constipation.
Effects of Ovarian Cycle on Hemodynamic Responses during Dynamic Exercise in Sedentary Women
Hyun-Min Choi,Charles L. Stebbins,Hosung Nho,Mi-Song Kim,Myoung-Jei Chang,Jong-Kyung Kim 대한생리학회-대한약리학회 2013 The Korean Journal of Physiology & Pharmacology Vol.17 No.6
This study tested the hypothesis that effects of the menstrual cycle on resting blood pressure carry over to dynamic exercise. Eleven healthy females were studied during the early (EP; low estrogen, low progesterone) and late follicular (LP; high estrogen, low progesterone) menstrual phases. Stroke volume (SV), heart rate (HR), cardiac output (CO), systolic blood pressure (SBP), diastolic blood pressure (DBP), and total vascular conductance (TVC) were assessed at rest and in response to mild and moderate cycling exercise during EP and LP. During EP, compared to LP, baseline SBP (111±1 vs. 103±2 mmHg), DBP (71±2 vs. 65±2 mmHg) and mean arterial pressure (MAP) (84±2 vs. 78±1 mmHg) were higher and TVC (47.0±1.5 vs. 54.9±4.2 ml/min/mmHg) was lower (p<0.05). During exercise, absolute values of SBP (Mild: 142±4 vs. 127±5 mmHg; Moderate: 157±4 vs. 144±5 mmHg) and MAP (Mild: 100±3 vs. 91±3 mmHg; Moderate: 110±3 vs. 101±3 mmHg) were also higher, while TVC was lower (Mild: 90.9±5.1 vs. 105.4±5.2 ml/min/mmHg; Moderate: 105.4±5.3 vs. 123.9±8.1 ml/ min/mmHg) during EP (p<0.05). However, exercise-induced increases in SBP, MAP and TVC at both work intensities were similar between the two menstrual phases, even though norepinephrine concentrations were higher during LP. Results indicate that blood pressure during dynamic exercise fluctuates during the menstrual cycle. It is higher during EP than LP and appears to be due to additive effects of simultaneous increases in baseline blood pressure and reductions in baseline TVC.
Hyun-Min Choi,Hosung Nho,In-Tae Ko,Chan-Ho Park,Jong-Kyung Kim 한국생활환경학회 2008 한국생활환경학회지 Vol.15 No.4-2
The renal vasoconstriction serves to shunt blood flow from the kidney toward active skeletal muscles during exercise. However, it is not well known that the stimulation of group Ⅲ muscle afferents reflexly increases sympathoexci-tation in the renal arteries. Therefore, we recorded single renal nerve filament response to tendon stretch before and after injection of gadolinium into the popliteal artery in decerebrate cats. In control experiment, the impulse activity of single renal nerve efferent was significantly increased during tendon stretch, while gadolinium significantly reduced the renal sympathetic impulse activity. The blood pressure responses induced by the mechanoreflex activation were significantly increased in both conditions, but the pressor response was substantially attenuated after injection of gadolinium. We conclude that the mechanical component of the exercise pressor reflex may contribute to evoke the renal vasoconstriction during exercise.
노양수,백종현,최영자,성호석,왕한영 인제대학교 1987 仁濟醫學 Vol.8 No.3
낙엽상 천포창은 천포창의 한 변형으로서 비교적 양성 결과를 취하는 드문 질환이다. 저자들은 유고슬라비아 백인에서 발생한 전형적인 낙엽상 천포창 1예을 경험하였기에 문헌 고찰과 함께 보고한다. We report a case of 34-year-old white man with pemphigus foliaceus, who developed characteristic skin lesions of flaccid bullae, erosions and crusts on the chest and abdomem. Both histopathologic study and direct immunofluorescence test were compatible with pemphigus foliaceus, showing intraepidermal bullae with acantholytic cells and fluorescence of intercelluar spaces of the epidermis, respectively.