Rosenvingea orientalis (Scytosiphonaceae, Phaeophyceae) from Chiapas, Mexico: life history in culture and molecular phylogeny

John A. West,Giuseppe C. Zuccarello,Francisco F. Pedroche,Susan Loiseaux de Goër 한국조류학회I 2010 ALGAE Vol.25 No.4

The genus Rosenvingea is well known in the tropics. Four species have been reported from Pacific Mexico: R. floridana,R. antillarum, R. intricata and R. sanctae-crucis. We collected a plant (Boca del Cielo, Chiapas) that we identified as Rosenvingea orientalis, a species not previously reported from Pacific Mexico. We were able to characterize the life cycle of this species for the first time in laboratory culture. It reproduced exclusively by plurilocular sporangia (plurangia). The mature plants were up to 6 cm long with cylindrical to compressed fronds (to 2 mm wide) with dichotomous branches in the upper half of the thallus. The medulla was hollow with 2-3 layers of large inflated colourless cells at the periphery. The cortex was comprised of 1 layer of small cells, each with a single chloroplast and pyrenoid. Linear plurangial sori with phaeophycean hairs formed along the mature fronds. Zoospore germlings developed into prostrate filamentous systems, each with a single phaeophycean hair that gave rise to a single erect shoot with multiple hairs arising near the tip. Molecular phylogeny using the psaA gene placed this isolate within the Scytosiphonaceae. It does not confirm the exact identification of R. orientalis, although its placement close to other Rosenvingea sequences was confirmed and morphological evidence supports its placement in R. orientalis. Our culture investigations indicated that it has an asexual life cycle. Further collections are needed to resolve the full generic and specific relationships of Rosenvingea and related taxa, and their reproductive patterns.

In vitro 방법에 의한 Alfalfa 엽 분미의 HCHO 처리 효과 실험

강희신,John R. Ashes 한국낙농학회 1985 韓國酪農學會誌 Vol.7 No.2

三年餘 貯藏되었던 Alfalfa 葉 粉末에 ^(14)C-HCHO를 其 合有 蛋白質의 0, 0.5, 1.0 및 2.0% 水準으로 處理하였을 때의 in vitro 減成化度를 測定하였는 바 다음과 같은 結果를 얻었다. (1) In vitro 蛋白質 減成化度는 12.7∼1.1%의 範圍에 있었으며 HCHO處理 水準 0, 0.5, 1.0, 및 2.0% 水準에 따라 각각 12.7, 12.0, 7.9 및 1.1%의 減成化度를 나타내었다. (2) HCHO의 處理는 醱酵 Tube內 溶液中의 揮發性 脂肪酸 濃度에 影響을 미치지 않았다. (3) ^(14)C-HCHO의 回收率은 平均 72.3%로서 基質의 蛋白質과 HCHO가 잘 結合되고 있음을 알게 되었다. In order to test the efficacy of HCHO treatment on alfalfa leaf meal for protecting its protein from degradation in in vitro fermentation, aged alfalfa leaf meal for about three years was treated by ^(14)C-HCHO at the level of 0, 0.5, 1.0 and 2.0% of protein content of the raw material and the protein degradability was measured with the amount of NH₄-N (㎎) over the total nitrogen presented per test tube after 24 hours incubation by using and incoculum collected from the sheep fed alfalfa pellet. The results obtained were summarized as follows; 1. The protein degradabilities measured were 12.7, 12.0, 7.9 and 1.1% for the ^(14)C-HCHO levels of 0, 0.5, 1.0 and 2,0% respectively. 2. No differences between treatments were found in the VFA concentration of the fermentation fluids in the tubes. 3. The recovery rates of protein bound formaldehyde from the treated alfalfa leaf meals were averaged 72.3%.

<i>Olpidiopsis</i> sp., an oomycete from Madagascar that infects <i>Bostrychia</i> and other red algae: Host species susceptibility

West, John A.,Klochkova, Tatyana A.,Kim, Gwang Hoon,Loiseaux-de Goë,r, Susan Blackwell Publishing Asia 2006 Phycological research Vol.54 No.1

<P>SUMMARY</P><P><I>Olpidiopsis</I> sp. (Oomycota) was cultured with its original host <I>Bostrychia moritziana</I> (Sonder ex Kützing) J. Agardh from Madagascar. Bean-shaped zoospores with two heterokont flagella attached to the host cell wall surface and in 2 days host cells began collapsing and one or more syncytia developed in each infected cell. Zoospores were cleaved and an exit tube with a small plug was formed. Complete development and zoospore discharge occurred in 3 days. Infection occurred in cells of polysiphonous branches, monosiphonous branches, rhizoids and reproductive stichidia. Dead cells of plants treated with microwave were not infected. Susceptibility was variable in other <I>Bostrychia</I> species from different countries. <I>Bostrychia moritziana</I> (Sonder ex Kützing) J. Agardh<I>,</I> and <I>Bostrychia radicans</I> (Montagne) Montagne from Madagascar were susceptible but one <I>Bostrychia tenella</I> (J. V. Lamouroux) J. Agardh isolate from Madagascar was susceptible and two were not. <I>B. radicosa</I> (Itono) J. A. West, G. C. Zuccarello et M. Hommersand isolates from Madagascar, Thailand, Australia and New Caledonia were susceptible but an isolate from Malaysia was not. <I>B. radicans</I> isolates from Mexico and Brazil were non-susceptible as were <I>Bostrychia flagellifera</I> Post, <I>Bostrychia harveyi</I> Montagne, <I>Bostrychia montagnei</I> Harvey, <I>Bostrychia simpliciuscula</I> Harvey ex J. Agardh<I>, Bostrychia tenuissima</I> R. J. King et Puttock<I>, Stictosiphonia intricata</I>(Bory de Saint-Vincent) P. C. Silva, <I>Stictosiphonia kelanensis</I> (Grunow) R. J. King et Puttock and <I>Stictosiphonia tangatensis</I> (Post) R. J. King et Puttock, <I>Lophosiphonia</I> sp., <I>Neosiphonia</I> sp. and <I>Polysiphonia</I> spp. isolates were also non-susceptible. Many non-susceptible strains showed initial cell-collapse followed by rapid wound-repair cell formation without syncytia or sporangia developing. <I>Caloglossa leprieurii</I> (Montagne) G. Martens from Madagascar showed cell-collapse and wound-repair in periaxial cells, but wing cells died and became purple without wound-repair. <I>Caloglossa ogasawaraensis</I> Okamura and <I>Caloglossa postiae</I> M. Kamiya et R. J. King had no symptoms of infection. <I>Dasysiphonia chejuensis</I> I. K. Lee et J. A. West was not infected. Surprisingly, the conchocelis phase but not the blade phase of <I>Porphyra pulchella</I> J. A.West, G. C. Zuccarello and <I>Porphyra suborbiculata</I> Kjellman was infected. The conchocelis of <I>Porphyra tenera</I> Kjellman and <I>Porphyra linearis</I> Greville were infected but no blade stages were tested. <I>Porphyra miniata</I> (C. Agardh) C. Agardh and <I>Porphyra dentata</I> Kjellman conchocelis were not infected. <I>Bangia atropurpurea</I> (Roth) C. Agardh gametophyte filaments were not infected. Other red, brown and green algae were not infected. Time lapse videomicroscopy of development and spore release was done.</P>

Heat Transfer and Pressure Loss for R407C

Xaiver Boissieux,R. Alan Johns,Morgan R. Heikal 대한기계학회 1998 Heat transter conference Vol.6 No.1

Experimental study on strengthening of R.C beam using glass fibre reinforced composite

Mini, K.M.,Alapatt, Rini John,David, Anjana Elizabeth,Radhakrishnan, Aswathy,Cyriac, Minu Maria,Ramakrishnan, R. Techno-Press 2014 Structural Engineering and Mechanics, An Int'l Jou Vol.50 No.3

This paper reports the influence of number of layers and length of GFRP sheets wrapped onto RCC beams for strengthening. Twelve beams of size $700mm{\times}150mm{\times}150mm$ were cast and tested. Two beams without GFRP and ten beams wrapped in different lay-up patterns with one and two layers of GFRP sheets was subjected to three point loading test and ultrasonic pulse velocity test. Initial crack load, ultimate failure load and types of failure have been observed and noted. Experimental results indicate a significant increase in initial and ultimate load carrying capacity of GFRP wrapped beams compared to unwrapped beams. The failed control specimen was retrofitted using U wrap scheme and tested under three point loading.

Experimental study on strengthening of R.C beam using glass fibre reinforced composite

K.M. Mini,Rini John Alapatt,Anjana Elizabeth David,Aswathy Radhakrishnan,Minu Maria Cyriac,R. Ramakrishnan 국제구조공학회 2014 Structural Engineering and Mechanics, An Int'l Jou Vol.50 No.3

This paper reports the influence of number of layers and length of GFRP sheets wrapped onto RCC beams for strengthening. Twelve beams of size 700mm × 150mm × 150mm were cast and tested. Two beams without GFRP and ten beams wrapped in different lay-up patterns with one and two layers of GFRP sheets was subjected to three point loading test and ultrasonic pulse velocity test. Initial crack load, ultimate failure load and types of failure have been observed and noted. Experimental results indicate a significantincrease in initial and ultimate load carrying capacity of GFRP wrapped beams compared to unwrapped beams. The failed control specimen was retrofitted using U wrap scheme and tested under three point loading.

On the genus Rhodella, the emended orders Dixoniellales and Rhodellales with a new order Glaucosphaerales (Rhodellophyceae, Rhodophyta)

Joe Scott,Eun Chan Yang,John A. West,Akiko Yokoyama,Hee Jeong Kim,Susan Loiseaux de Goër,Charles J. O’Kelly,Evguenia Orlova,김수연,Jeong Kwang Park,윤환수 한국조류학회I 2011 ALGAE Vol.26 No.4

The marine unicellular red algal genus Rhodella was established in 1970 by L. V. Evans with a single species R. maculata based on nuclear projections into the pyrenoid. Porphyridium violaceum was described by P. Kornmann in 1965 and transferred to Rhodella by W. Wehrmeyer in 1971 based on plastid features and the non-parietal position of the nucleus. Molecular and fine structural evidences have now revealed that Rhodella maculata and R. violacea are one species, so R. violacea has nomenclatural priority and the correct name is Rhodella violacea (Kornmann) Wehrmeyer. The status of families within Rhodellophyceae was examined. The order Dixoniellales and family Dixoniellaceae are emended to include only Dixoniella and Neorhodella. The order Rhodellales and family Rhodellaceae are emended to include Rhodella and Corynoplastis. Glaucosphaera vacuolata Korshikov and the Glaucosphaeraceae Skuja (1954) with an emended description are transferred to the Glaucosphaerales ord. nov.

The Millimeter Continuum Size-Frequency Relationship in the UZ Tau E Disk

Tripathi, Anjali,Andrews, Sean M.,Birnstiel, Tilman,Chandler, Claire J.,Isella, Andrea,Pé,rez, Laura M.,Harris, R. J.,Ricci, Luca,Wilner, David J.,Carpenter, John M.,Calvet, N.,Corder, S. A.,Del American Astronomical Society 2018 The Astrophysical journal Vol.861 No.1

<P>We present high spatial resolution observations of the continuum emission from the young multiple star system UZ Tau at frequencies from 6 to 340 GHz. To quantify the spatial variation of dust emission in the UZ Tau E circumbinary disk, the observed interferometric visibilities are modeled with a simple parametric prescription for the radial surface brightnesses at each frequency. We find evidence that the spectrum steepens with radius in the disk, manifested as a positive correlation between the observing frequency and the radius that encircles a fixed fraction of the emission (R-eff proportional to nu(0.34 +/- 0.08)). The origins of this size-frequency relation are explored in the context of a theoretical framework for the growth and migration of disk solids. While that framework can reproduce a similar size-frequency relation, it predicts a steeper spectrum than that observed. Moreover, it comes closest to matching the data only on timescales much shorter (<= 1 Myr) than the putative UZ Tau age (similar to 2-3 Myr). These discrepancies are direct consequences of the rapid radial drift rates predicted by models of dust evolution in a smooth gas disk. One way to mitigate that efficiency problem is to invoke small-scale gas pressure modulations that locally concentrate drifting solids. If such particle traps reach high-continuum optical depths at 30-340 GHz with a similar to 30%-60%. filling fraction in the inner disk (r less than or similar to 20 au), they can also explain the observed spatial gradient in the UZ Tau E disk spectrum.</P>

Potent in vitro and in vivo activity of an Fc-engineered humanized anti-HM1.24 antibody against multiple myeloma via augmented effector function

Tai, Yu-Tzu,Horton, Holly M.,Kong, Sun-Young,Pong, Erik,Chen, Hsing,Cemerski, Saso,Bernett, Matthew J.,Nguyen, Duc-Hanh T.,Karki, Sher,Chu, Seung Y.,Lazar, Greg A.,Munshi, Nikhil C.,Desjarlais, John R American Society of Hematology 2012 Blood Vol.119 No.9

<B>Abstract</B><P>HM1.24, an immunologic target for multiple myeloma (MM) cells, has not been effectively targeted with therapeutic monoclonal antibodies (mAbs). In this study, we investigated in vitro and in vivo anti-MM activities of XmAb5592, a humanized anti-HM1.24 mAb with Fc-domain engineered to significantly enhance FcγR binding and associated immune effector functions. XmAb5592 increased antibody-dependent cellular cytotoxicity (ADCC) several fold relative to the anti-HM1.24 IgG1 analog against both MM cell lines and primary patient myeloma cells. XmAb5592 also augmented antibody dependent cellular phagocytosis (ADCP) by macrophages. Natural killer (NK) cells became more activated by XmAb5592 than the IgG1 analog, evidenced by increased cell surface expression of granzyme B-dependent CD107a and MM cell lysis, even in the presence of bone marrow stromal cells. XmAb5592 potently inhibited tumor growth in mice bearing human MM xenografts via FcγR-dependent mechanisms, and was significantly more effective than the IgG1 analog. Lenalidomide synergistically enhanced in vitro ADCC against MM cells and in vivo tumor inhibition induced by XmAb5592. A single dose of 20 mg/kg XmAb5592 effectively depleted both blood and bone marrow plasma cells in cynomolgus monkeys. These results support clinical development of XmAb5592, both as a monotherapy and in combination with lenalidomide, to improve patient outcome of MM.</P>

Variations in the origin of the anterior spinal artery in a Colombian population sample

Jorge Eduardo Duque Parra,John Barco Ríos,Juan Fernando Vélez García 대한해부학회 2022 Anatomy & Cell Biology Vol.55 No.4

Based on most neuroanatomical descriptions, the anterior spinal artery (ASA) originates from two small vessels of the vertebral arteries, which are anastomosed just before forming the basilar artery. This study aimed to determine and quantify the possible variants of the origin of the ASA and its trajectory in samples of human brain stems. Male brain stems with the superior portion of the spinal cord until myelomera C3 of 23 adult human, and no evident morphological alterations were selected. The brain stems were collected for three years and fixed in a 10% formalin solution at the Anatomy Laboratory of the Universidad de Caldas (Colombia). Five samples (21.7%) had variations in the origin and trajectory of the anterior spinal artery. The variations in the origin of the ASA could generate morphofunctional advantages instead of leading to complications. That is the case when there are two anterior spinal arteries since it would increase tissue perfusion, thus protecting part of the spinal cord from ischemic pathologies. It is essential to consider the variations that may exist in the supply of the anterior region of the spinal cord for clinical and surgical assessments due to variations in its supplied territory.