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강희신,이성실 한국낙농학회 1992 韓國酪農學會誌 Vol.14 No.2
收穫 時期別 아카시아 分劃物이 反芻胃內 分解 特性에 어떻게 작용하는가를 叫明하고자, 3×3要因實驗法에 의하여, 主要因(3 收穫時期 ; J=6月, A=8月 및 0=10月)과 副要因(3 採取 部位 ; L+P=葉+葉柄, S=줄기 및 WPM=葉, 줄기 및 葉柄의 혼합물)을 설정하고 반추위 주관이 施術된 緬羊(Corriedale×Polworth)交雜種 수컷(♂) 3두를 反覆으로 하여, Nylon bag內의 試料가 反芻胃에 懸垂 醱酵되는 동안(즉, 0, 6, 12, 24, 48 및 72시간)의 試料中 DM 및 OM 分解 特性이 調査 되었는바, 그 結果를 要約하면 다음과 같다. 1. 乾物 消失率(Dry Matter Disappearance Rate, DMDR, %) 및 그 分解度(Dry Matter Degradability, DMD, %) 1) 試料의 DMDR(%)을 部位別(L+P,S 및 WPM)로 볼 때, 全 醱酵 期間中 有意差(p<0.01)가 認定되며, 各各 13.2∼55.1, 8.1∼31.2 및 10.3∼43.0%로서 L+P試料는 餘他 두 試料 보다 越等히 높게 나타났고, S試料는 가장낮은 消失率을 나타내었다. 2) 各 試料의 經時的(t) 分解度 推定値(P%)는 다음의 各 函數式으로 誘導되었다. P( %, L+P) = 14.56+40.92(1-e^(-0.06268t)) P( %, S) = 8.05+25.75(1-e^(-0.04027t)) P( %,WPM) = 10.91+33.32(1-e^(-0.05406t)) 3) 部位別 試料 乾物의 有效分解度(Effective Degradability, DEG %)는 試料 : L+P, S 및 WPM이, 各各 39.54, 20.97 및 30.06% 이었으며, 收穫時期別 試料 : J, A 및 O는 各各 35.55, 29.89 및 26.89%로 算出되었다. 4) 試料의 DMDR(%)을 收穫 時期別(J, A 및 O)로 볼 때, 全 醱酵 期間에 걸쳐서, 有意差(p<0.01)가 認定되었으며, 그 값은, 各各 11.5∼48.4, 12.0∼38.7 및 8.1∼42.2%의 範圍로서, 全醱酵期間 中 平均 31.3%가 消失되었다. 한편, J試料의 平均値 31.3%는 가장 높았고, A試料의 平均値 24.7%는 相互間 有意差(p<0.01)가 認定된다. 5) 收穫 時期別 試料 乾物의 經時的(t) 分解度 推定値(P, %)는 다음 各各와 函數式으로 誘導되었다. P(%, J)=10.82+39.54(1-e^(-0.05406t)) P(%, A)=11.31+31.05(1-e^(-0.06268t)) P(%, O)=11.91+29.45(1-e^(-0.04027t)) 2. 有機物의 消失率(Organic Matter Disappearance Rate, OMDR %)과 그 分解度(Organic Matter Degradability, OMD %) 1) 試料 L+P, S 및 WPM의 有機物 消失率(OMDR, %)은 各各 11.8∼54.0%, 7.6∼30.9% 및 11.5∼54.0%의 範團內에 있었으며, L+P의 試料가 가장 높은 消失率을 나타내어 S와 WPM 試料 보다 各各 1.85倍 및 1.37倍가 더 消失되었다. P(%, L+P)=12.11+41.67(1-e^(-0.06599t)) P(%, S) =7.05+26.18(1-e^(-0.04017t)) P(%, WPM)=11.57+32.02(1-e^(-0.04027t)) 2) 分劃物別 試料의 經時的(t) 有機物 分解度 推定値(P, %)는 各各 다음과 같은 函數式으로 誘導되었다. P(%, L+P)=12.11+41.67(1-e^(-0.06599t)) P(%,S) =7.05+26.18(1-e^(-0.04017t)) P(%, WPM)=11.57+32.02(1-e^(-0.04027t)) 3) 分劃物 別(L+P, S 및 WPM)試料의 有機物 有效 分解度(DEG, %)는 各各 38.05, 20.31 및 27.63%로 測定 되었으며, 收穫 時期別 試料 : 卽, J, A 및 O 試料의 것은 各各 26.92, 24.25 및 32.24%로 測定되었다. 4) 收穫 時期別(J, A, O.)試料의 有機物 消朱率은 各各 7.2∼55.2, 3.6∼52.0 및 10.5∼49.3%의 範圍로서, 그 平均 값은 J 및 A 試料가 25%로서 비슷하였으나, O 誠料는 30%로서 여름철의 것(J 및 A試料)보다 20%程度 더 分解 되어 高度의 有意差(p<0.01)가 認定된다. 5) 收穫 時期別(J, A 및 O)試料의 經時的(t) 有機物 分解度 測定値(P,%)는 各各 다음과 같은 函數式으로 誘導되었다. P(%, J)=8.35+32.38(1-e^(-0.0523t)) P(%, A)=10.68+31.70(1-e^(-0.04027t)) P(%, O)=12.62% +34.69(1-e^(-0.05203t)) A 3 × 3 factorial experiment with three parts of acacia fractionates : L+P(leaf+petiloe), S (stem alone) and WPM(Whole plant mixtures : L+P+S) and three harvesting periods : J(July), A(August) and O(October) were performed to examine the effects of harvesting periods on the degradation characteristics of acacia fractionates by nylon bag techniques in the rumen of the sheep fed grass hay. Three Corriedale X Polworth cross breed male sheep equipped with the rumen fistulae weighting average 40.2kg were employed to measure the DMDR(Dry matter disappearance rates, %) and OMDR(Organic matter disappearance rates, % ) with respective nine replications according to the hours of suspension for 0, 6, 12, 24, 48 and 72 hrs in the rumen. Results obtained were summarized as the followings ; 1. The DMDR( % ) of acacia fractionates ranged from 13.2 to 55.1% for L+P, from 8.1 to 31.2% for S, and from 10.3 to 43.0% for WPM during the hours of suspension in the rumen. The mean DMDR( % ) of 3 fractionates was shown to have significant ( P<0.01) difference among them, and that of L+P showed the highest DMDR of 36.6 % and 28.0% and 19.7% for S and WPM, respectively. These values were shown to be increased significantly(P<0.01) by extending the hours of suspension. The estimated potential degradation rates(P) of DMD(%) for L+P, S and WPM were subjected to derive a single exponential equations by an iterative least square procedures, and after the lack of fit test, the following equations were obtained. P(L+P)=14.56+40.92(1-e^(-0.06268t)), P( S )= 8.05+25.75(1-e^(-0.04027t)), P( WPM )=10.91+33.32(1-e^(-0.05406t)). And, the effective degradabilities(DEG, %) of DM were 39.54, 20.97 and 30.06% for L+ P, S and WPM of acacia fractionates, respectively, with the assumed "k" value of 0.04h^(-1). 2. The effect of harvesting periods on the DMDR(%) of acacia fractionates was ranged from 11.5 to 48.4%, 12.0 to 38.7% and 8.1 to 42.2% for J, A and O, respectively. The DMDR(%) in J was the highest value of 31.1 %, and was declined to the lowest value of 24.7%, and thereafter regained to 28.2% in A and O test feeds. And, the equations obtained were also presented as follows ; P(J)=10.82+39.54(1-e^(-0.05406t)), P(A)=11.31+31.05(1-e^(-0.06268t)), P(O)=11.91+29.45(1-e^(-0.04027t)). The DEG(%) of DM was calculated to be of 35.55, 29.89 and 26.89% for acacia harvested in J, A, and O, with the assumed "k" value of 0.04h^(-1). 3. The OMDR(%) of acacia fractionates varied between 11.8-54.0%, 7.6-30.9% and 11.5-54.0% for L+P, S and WPM, respectively. The highest OMDR of L+P was 1.85 and 1.37 times more disappearable than that of S and WPM, respectively. The equations obtained were also presented as follows. P(L+P)=12.11+41.67(1-e^(-0.06599t)), P( S )= 7.05+26.18(1-e^(-0.04027t)), P(WPM)=11.57+32.02(1-e^(-0.04027t)). The DEG (%) of OM was 38.05, 20.31 and 27.63% for L+P, S and WPM of acacia fractionates, respectively, with the assumed "k" value of 0.04h^(-1). 4. The OMDR(%) of acacia fractionates according to the stage of harvesting varied between 10.1-39.6%, 11.6-40.1% and 9.1-46.7% for J, A and O, respectively. The OMDR of acacia harvested in J and A showed similar value of, however, that in O, did show as much as 30% which was about 20% higher than that of A. The OMDR of test feeds harvested in different stages of maturity was allowed to be expressed as the following equation : P(J)= 8.05+32.48(1-e^(-0.05230t)), P(A)=10.68+31.70(1-e^(-0.04027t)), P(O)=12.62+34.69(1-e^(-0.04027t)). The DEG(%) of OM was 26.92, 24.25 and 32.23% for acacia harvested in J, A and O, respectively, with the assumed "k" value of 0.04h^(-1).