Methylobacterium terrae sp. nov., a radiation-resistant bacterium isolated from gamma ray-irradiated soil

Jiyoun Kim,Geeta Chhetri,Inhyup Kim,Hyungdong Kim,Myung Kyum Kim,Taegun Seo 한국미생물학회 2019 The journal of microbiology Vol.57 No.11

A Gram-stain-negative, asporogenous, aerobic rods, motile by means of a single polar flagellum, catalase- and oxidase-positive, methylotrophic bacterium, designated 17Sr1-28T, was isolated from gamma ray-irradiated soil. The 16S rRNA gene sequence analysis showed that strain 17Sr1-28T was phylogenetically related to Methylobacterium currus PR1016AT (96.8%), Methylobacterium platani PMB02T (96.2%), Methylobacterium aquaticum DSM 16371T (96.3%), Methylobacterium tarhaniae N4211T (96.4%), Methylobacterium frigidaeris IER25-16T (95.8%), and Methylobacterium organophilum JCM 2833T (92.7%). The G+C content calculated based on genome sequence was 71.6%. The average nucleotide identity and in silico DNA-DNA hybridization values between strain 17Sr1- 28T and M. currus, M. platani, M. aquaticum, M. tarhaniae, M. frigidaeris, and M. organophilum were 77.7–90.4% and 22–39.6%, respectively. The major fatty acids of strain 17Sr1- 28T were summed feature 8 (C18:1 ω7c and/or C18:1 ω6c), and summed feature 3 (C16:1 ω7c and/or C16:1 ω6c). The predominant quinone was ubiquinone 10 and the major polar lipids were diphosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, and phosphatidylglycerol. On the basis of the data from phenotypic tests and genotypic differences between strain 17Sr1-28T and its close phylogenetic relatives, strain 17Sr1-28T represents a new species belonging to the genus Methylobacterium, for which the name Methylobacterium terrae sp. nov. (= KCTC 52904T = NBRC 112873T) is proposed.

A Case Study on Physical Characteristics of High School Male Elite Athletes according to Sport Type: Focusing on the Target Elite Athletes of Seoul Sports Science Research Institute

( Jiyoun Kim ),( Eunsurk Yi ),( Munhee Kim ),( Ahra Oh ),( Jongseob Shin ),( Namyoung Kim ) 융합연구학회 2020 융합연구학회지 Vol.4 No.2

Since 2015, Korea has been operating regional sports science centers in 10 cities across the country, and is pursuing a plan to systematically link athletes’ physical fitness through physical fitness measurements according to the athlete’s life cycle. For 243 elite athletes whose physical strength was measured by the Seoul Sports Science Center, the development direction of each sport type of the elite athletes in Seoul is analyzed through classification and grade. As a result of the study, in terms of basic physical strength, the difference in physical strength by sport type was significant in all items of muscle strength, muscular endurance, muscle power, cardiopulmonary endurance, agility, balance, and coordination except flexibility. As a result of analyzing the difference in the level of the physical strength factor according to the application of the fitness grade criteria for each sport type, the A type showed significantly higher differences in grip strength and back strength than the B and D types. It was significantly higher in sit-up than type A. Type B showed significantly higher physical endurance, muscle power, cardiopulmonary endurance, agility, coordination, and equilibrium compared to the A and D types, and D type showed higher physical strength level than the A type in cardiopulmonary endurance, agility, and balance. Based on the results of this study, it is considered necessary to compare the performance and physical strength of male high school students in Seoul and the characteristics of athletes by sport type to review the training method and improvement of the performance.

전경골건 부분 이식을 이용한 장족무지신건 퇴행성 파열의 치료 사례

Kim Jiyoun,Kim Gab-Lae,Kim Taeho 대한족부족관절학회 2022 대한족부족관절학회지 Vol.26 No.4

Chronic extensor hallucis longus (EHL) tendon rupture is relatively rare, but in such cases, surgical repair is necessary to prevent hallux dysfunction. To the best of our knowledge, reconstruction of chronic EHL rupture using a split tibialis anterior tendon autograft has not been previously reported. Here we present a case of spontaneous EHL tendon rupture with a 5 cm gap in a healthy 57-year-old woman. At the 1-year follow-up evaluation, hallux function was restored, and the patient was well satisfied with results.

Preparation and Polymerization Behavior of Polymeric Dental Restorative Materials Containing High Molecular Weight Diluent System

Kim, Ohyoung,Chun, Jiyoun,Kim, Yongwoon,Shim, W. Jaewoo 한국공업화학회 2003 Journal of Industrial and Engineering Chemistry Vol.9 No.6

Preparation of polymeric dental restorative materials (PDRM) showing a lower polymerization shrinkage was carried out using the hybrid-filler and 2,2'-bis-[4-(3-methacryloxy-2-hydroxypropoxy) phenyl]propane resin matrix that is diluted with a high molecular weight dimethacrylate monomer. A visible light system was utilized to activate the resin matrix for the polymerization. In order to enhance the miscibility of the inorganic hybrid-filler with organic resin matrix and to conduct the homogeneity of the filler in the PDRM, its surface was hydrophobically treated with y -methacryloxypropyltrimethoxysilane. The degree of conversion (DC), depth of cure, and polymerization shrinkage of the PDRM were investigated. The results revealed that PDRM prepared herein showed markedly lower polymerization shrinkage values. Besides, regardless of the filler loading, both DC and depth of cure values decreased with an increase in the amount of less flexible diluent in the resin matrix.

Binary Power Amplifier with 2-Bit Sigma-Delta Modulation Method for EER Transmitter

Jiyoun Lim,Sanghoon Cheon,Kyeong-Hak Kim,홍성철,Dong-Wook Kim 한국전자통신연구원 2008 ETRI Journal Vol.30 No.3

A novel power amplifier for a polar transmitter is proposed to achieve better spectral performance for a wideband envelope signal. In the proposed scheme, 2-bit sigma-delta (ΣΔ) modulation of the envelope signal is introduced, and the power amplifier configuration is modified in a binary form to accommodate the 2-bit digitized envelope signals. The 2-bit ΣΔ modulator lowers the noise of the envelope signal by fine quantization and thus enhances the spectral property of the RF signal. The Ptolemy simulation results of the proposed structure show that the spectral noise is reduced by 10 dB in a full transmit band of the EDGE system. The dynamic range is also enhanced. Since the performance is improved without increasing the over-sampling ratio, this technique is best suited for wireless communication with high data rates.

Synthesis and biological evaluation of 3-(2-aminoethyl) uracil derivatives as gonadotropin-releasing hormone (GnRH) receptor antagonists

Kim, Seon-Mi,Lee, Minhee,Lee, So Young,Lee, Soo-Min,Kim, Eun Jeong,Kim, Jae Sun,Ann, Jihyae,Lee, Jiyoun,Lee, Jeewoo Elsevier 2018 European journal of medicinal chemistry Vol.145 No.-

<P><B>Abstract</B></P> <P>We investigated a series of uracil analogues by introducing various substituents on the phenyl ring of the <I>N</I>-3 aminoethyl side chain and evaluated their antagonistic activity against human gonadotropin-releasing hormone (GnRH) receptors. Analogues with substituents at the ortho or meta position demonstrated potent in vitro antagonistic activity. Specifically, the introduction of a 2-OMe group enhanced nuclear factor of activated T-cells (NFAT) inhibition up to 6-fold compared to the unsubstituted analogue. We identified compound <B>12c</B> as a highly potent GnRH antagonist with moderate CYP inhibition. Compound <B>12c</B> showed potent and prolonged LH suppression after a single dose was orally administered in castrated monkeys compared to a known antagonist, Elagolix. We believe that our SAR study offers useful insights to design GnRH antagonists as a potential treatment option for endometriosis.</P> <P><B>Highlights</B></P> <P> <UL> <LI> A series of 3-(2-aminoethyl) uracil analogues were synthesized as GnRH antagonists. </LI> <LI> Compound <B>12c</B> showed highly potent GnRH antagonism with moderate CYP inhibition. </LI> <LI> Compound <B>12c</B> exhibited potent and prolonged LH suppression in castrated monkeys. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

Discovery of an Orally Bioavailable Gonadotropin-Releasing Hormone Receptor Antagonist

Kim, Seon-Mi,Lee, Minhee,Lee, So Young,Park, Euisun,Lee, Soo-Min,Kim, Eun Jeong,Han, Min Young,Yoo, Taekyung,Ann, Jihyae,Yoon, Suyoung,Lee, Jiyoun,Lee, Jeewoo American Chemical Society 2016 Journal of medicinal chemistry Vol.59 No.19

<P>We developed a compound library for orally available gonadotropin-releasing hormone (GnRH) receptor antagonists that were based on a uracil scaffold. On the basis of in vitro activity and CYP inhibition profile, we selected 18a (SKI2496) for further in vivo studies. Compound 18a exhibited more selective antagonistic activity toward the human GnRH receptors over the GnRHRs in monkeys and rats, and this compound also showed inhibitory effects on GnRH-mediated signaling pathways. Pharmacokinetic and pharmacodynamic evaluations of 18a revealed improved bioavailability and superior gonadotropic suppression activity compared with Elagolix, the most clinically advanced compound. Considering that 18a exhibited highly potent and selective antagonistic activity toward the hGnRHRs along with favorable pharmacokinetic profiles, we believe that 18a may represent a promising candidate for an orally available hormonal therapy.</P>

Isolation of Angiotensin Converting Enzyme Inhibitors from Compositae Plants

Jiyoun Kim,Hyeyoung Jung,Kyung Bin Song 한국식품영양과학회 2002 Preventive Nutrition and Food Science Vol.7 No.2

Thirty plants of the Compositae family were screened for inhibitory activity of angiotensin converting enzyme (ACE). Among them, Chrysanthemum boreale Makino and Ixeris dentate Nakai were selected for further investigation since they had the highest inhibitory activity. Crude water extracts of the flowers of Chrysanthemum boreale Makino and the roots of Ixeris dentate Nakai were prepared by heating at 95℃ and 60℃ for 2 hr, respectively, followed by centrifugation at 8000×g for 30 min. Crude extracts were then filtered using YM-3 and YM-1 membranes. The ACE inhibitors were isolated using consecutive chromatographic methods including: Sephadex G-15, ion exchange, FPLC, and reverse phase HPLC. The inhibitors were identified to have molecular masses of 204 and 283 daltons, respectively, by mass spectrometry. These results demonstrate that crude extracts of Compositae plants may be useful as functional food ingredients with anti-hypertensive properties.

족부족관절 분야의 환자 자가 보고 결과 평가법

Kim Jiyoun,Kim Bom Soo 대한족부족관절학회 2022 대한족부족관절학회지 Vol.26 No.1

The patient-reported outcome measures (PROMs) are important in assessing the patient’s overall health, lesion-specific outcomes, and condition-specific outcomes. PROMs are also known as “scoring systems” and are usually in questionnaires. There are almost a hundred different PROMs available in foot and ankle surgery. Each PROM has its merits, demerits, and validity. Selecting an appropriate PROM is important for adequately evaluating a patient’s health status. This article summarizes the most frequently used PROMs in the literature on foot and ankle surgery and presents the authors’ recommendations.

Cellular phosphatase activity of C1-Ten/Tensin2 is controlled by Phosphatidylinositol-3,4,5-triphosphate binding through the C1-Ten/Tensin2 SH2 domain

Kim, Eui,Kim, Do-Hyeon,Singaram, Indira,Jeong, Heeyoon,Koh, Ara,Lee, Jiyoun,Cho, Wonhwa,Ryu, Sung Ho Elsevier 2018 Cellular signalling Vol.51 No.-

<P><B>Abstract</B></P> <P>Regulation of tyrosine phosphorylation on insulin receptor substrate-1 (IRS-1) is essential for insulin signaling. The protein tyrosine phosphatase (PTP) C1-Ten/Tensin2 has been implicated in the regulation of IRS-1, but the molecular basis of this dephosphorylation is not fully understood. Here, we demonstrate that the cellular phosphatase activity of C1-Ten/Tensin2 on IRS-1 is mediated by the binding of the C1-Ten/Tensin2 Src-homology 2 (SH2) domain to phosphatidylinositol-3,4,5-trisphosphate (PtdIns(3,4,5)P<SUB>3</SUB>). We show that the role of C1-Ten/Tensin2 is dependent on insulin-induced phosphoinositide 3-kinase activity. The C1-Ten/Tensin2 SH2 domain showed strong preference and high affinity for PtdIns(3,4,5)P<SUB>3</SUB>. Using site-directed mutagenesis, we identified three basic residues in the C1-Ten/Tensin2 SH2 domain that were critical for PtdIns(3,4,5)P<SUB>3</SUB> binding but were not involved in phosphotyrosine binding and PTP activity. Using a PtdIns(3,4,5)P<SUB>3</SUB> binding-deficient mutant, we showed that the specific binding of the C1-Ten/Tensin2 SH2 domain to PtdIns(3,4,5)P<SUB>3</SUB> allowed C1-Ten/Tensin2 to function as a PTP in cells. Collectively, our findings suggest that the interaction between the C1-Ten/Tensin2 SH2 domain and PtdIns(3,4,5)P<SUB>3</SUB> produces a negative feedback loop of insulin signaling through IRS-1.</P> <P><B>Highlights</B></P> <P> <UL> <LI> C1-Ten's function as a PTP for IRS-1 in insulin signaling is controlled by PI3K. </LI> <LI> The C1-Ten SH2 domain binds to PtdIns(3,4,5)P<SUB>3</SUB> with high affinity. </LI> <LI> The PtdIns(3,4,5)P<SUB>3</SUB> binding of C1-Ten is necessary for its function as PTP. </LI> </UL> </P>