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박지수(Jisu Park),소선섭(Sun Sup So),은성배(Seongbae Eun) 한국정보통신학회 2021 한국정보통신학회 종합학술대회 논문집 Vol.25 No.2
비교적 저렴한 가격으로 가까운 거리를 빠르게 이동할 수 있는 공유기반 전동킥보드의 이용률이 크게 향상되고 있다. 문제는 전동킥보드가 적절하지 않은 공간에 주차되어 안전사고를 유발하는 것이다. 본 논문에서는 딥러닝 기반 객체 인식 기술을 적용하여 방치된 전동킥보드의 잘못된 주차를 인식하는 체계를 제안한다. 본 논문에서는 실험 데이터의 특성을 고려하여 CNN과 유사한 모델을 별도로 생성하였으며 실험을 통하여 60%의 인식률을 얻었음을 보였다. The use of shared electric kickboards that can move quickly within a short distance at a relatively low price is increasing significantly. In this paper, we propose a system for recognizing incorrect parking of an abandoned shared kickboard by applying deep learning-based object recognition technology. In this paper, a model similar to CNN was created separately considering the characteristics of the experimental data, and it was shown that a recognition rate of 60% was obtained through the experiment.
국소구간 배출량 산정을 위한 속도 및 부하에 따른 배출계수 개발
박지수(Jisu Park),서지구(Jigu Seo),박준홍(Junhong Park),박성욱(Sungwook Park) 한국자동차공학회 2021 한국자동차공학회 부문종합 학술대회 Vol.2021 No.6
배출계수는 대기환경 분야의 연구 및 정책기반 자료로써 활용 가치가 높으며 차량의 종류 및 연식 규모, 유종에 따라 세분화된 배출계수가 지역 및 국가 배출량 산정에 활용되고 있다. 국내의 환경부는 자동차의 실도로 배출 특성을 파악하고 정량적으로 분석하기 위해 대기오염물질 배출원을 체계적으로 분류하고 배출원별 활동자료와 배출계수를 기초로 하는 인벤토리(CAPSS, Clean Air Policy System)를 구축하여 매년 다양한 배출원의 배출량을 산정하고 있다. CAPSS는 단위 이동거리당 자동차 배출량(g/km)을 차속(km/h)의 함수로 나타내는데, CAPSS에서 활용된 속도와 배출량의 관계는 다수의 차량 시험 데이터를 기반으로 회귀분석을 통해 개발되었다. 속도 기반 배출계수는 사용이 간단하다는 장점이 있으나, 배출량을 산정할 때 차속만을 변수로 고려하기 때문에, 동일한 차속 구간에서는 모두 동일한 배출량이 산출되고 다양한 형태의 가감속 및 주행 특성을 반영할 수 없다는 단점이 있다. 따라서 CAPSS 배출계수는 주로 국가 수준의 대규모 배출량과 같은 Macro-scale 배출량 산정 시에만 유용하나, 차량의 가감속 및 다양한 운전형태의 고려가 필요한 Micro-scale 배출량 산정에는 적용하기 어렵다는 한계점이 있다. 평균속도만의 함수로 배출계수를 산정하는 우리나라의 CAPSS 배출계수 산정방식과 다르게 미국 EPA MOVES(Motor Vehicle Emission Simulator)는 초당 측정된 차량의 속도 및 비출력(VSP, Vehicle Specific Power)을 반영한 배출계수로 차량의 다양한 주행 상태(Operating Mode)를 반영할 수 있도록 하고 있다. 하지만 미국과 국내의 차량 구분법 및 차량 타입, 배출가스규제 등 배출계수 수준이 우리나라와 다르기 때문에 MOVES에서 활용하고 있는 속도-비출력 기반 배출계수 체계가 국내에도 적용 가능한지에 대한 사전 연구가 필요하다. 본 연구에서는 해외 배출계수 산정 사례인 EPA의 MOVES 배출계수 산정 방법을 반영하여 소형자동차의 배출가스규제 및 유종, 배기량에 따른 배출계수를 개발하여 실내 주행시험 및 실도로 주행시험 결과와 비교를 통해 검증한 후 기존의 CAPSS 배출계수 산정방식 대비 배출계수의 정확도를 높이며, 국소구간 배출량 산정에 적용가능한 배출계수를 개발하고자 한다.
Park, Yong Jin,Jeong, Jisu,Kim, Jae Seok,Choi, Dong Soo,Cho, Goang-Won,Park, Jin Seong,Lim, Jong Kuk The Basic Science Institute Chosun University 2019 조선자연과학논문집 Vol.12 No.2
Staphylococcus epidermidis is well-known not only as an innocuous normal flora species commonly isolated from human skin, but also as an important bacterial species to keep skin healthy, because this species can protect the human skin from pathogenic microorganisms. However, S. epidermidis turns into a potential pathogen in damaged skin, because these bacteria can easily form a biofilm on the wound area and provide antimicrobial resistance to other microorganisms embedded in the biofilm. Thus, it is important to kill S. epidermidis in the early stage of wound treatment and block the formation of biofilms in advance. In the present study, hydrogel wound dressings were fabricated using polyvinyl alcohol/polyethylene glycol containing silver citrate nanorods, which have been proven to have strong antimicrobial activity, especially against S. epidermidis, and their wound-healing efficacy was investigated in vivo using a rat experiment.
Substrate Specificity Analysis of Reductases for Hydroxy Acid Biosynthesis
Jisu PARK,Hoe-Suk LEE,Hae Ji JEON,Han Sol KIM,Taejung KIM,Jungyeob HAM,Young Joo YEON,Young-Tae PARK 한국생물공학회 2021 한국생물공학회 학술대회 Vol.2021 No.10
Hydroxy acids are compounds referred to as organic acids with a hydroxyl group, and are widely used in industries such as medicine, food, cosmetics, and chemical products. 3-Hydroxybutyrate dehydrogenase (3HBDH) from Alcaligenes faecalis can catalyze 3-oxobutyrate, also known as acetoacetate. Therefore, using rational design with mutational studies, 3HBDH had been engineered to convert 4-oxovalerate, also known as levulinic acid, into 4-hydroxyvalerate. In this study, activities for keto acid substrates with variable carbon chain length (C3-C8) were measured, and substrate specificity was analyzed through structural analysis. The engineered 3HBDH was able to react with all candidate substrates, while the wildtype 3HBDH reacted with only a few. Analyzing the distance between the substrate and the enzyme by docking simulation, Ser142 and Tyr155 were directly related to the catalytic mechanism. In addition, Gln196 appeared to be involved in the enzyme-substrate complex by binding hydrogen bonds with ketone and carboxyl groups of the substrate. Based on the results of this study, data for engineering strategy of a redox enzyme platform for synthesizing organic acids were obtained.
Park, Hun-Young,Kim, Jisu,Park, Mi-Young,Chung, Nana,Hwang, Hyejung,Nam, Sang-Seok,Lim, Kiwon Korean Society for the Study of Obesity 2018 Journal of obesity & metabolic syndrome Vol.27 No.2
<P>Obesity is an important health problem caused by positive energy balance. Generally, low calorie dietary intake combined with regular exercise is the most common modality to lose bodily fat in obese people. Although this is the first modality of choice for obesity treatment, it needs to be applied to obese patients for at least 12 weeks or more and it does not provide consistent results because it is difficult to suppress increased appetite due to exercise. Recently, many researchers have been applying hypoxic conditions for the treatment of obesity, as many studies show that people residing in high altitudes have a lower percentage of body fat and fewer obesity-related illnesses than people living at sea level. Hypoxic therapy treatment, including hypoxic exposure or hypoxic exercise training, is recommended as a way to treat and prevent obesity by suppression of appetite, increasing basal metabolic rate and fat oxidation, and minimizing side effects. Hypoxic therapy inhibits energy intake and appetite-related hormones, and enhances various cardiovascular and metabolic function parameters. These observations indicate that hypoxic therapy is a new treatment modality for inducing fat reduction and promoting metabolic and cardiovascular health, which may be an important and necessary strategy for the treatment of obesity. As such, hypoxic therapy is now used as a general medical practice for obesity treatment in many developed countries. Therefore, hypoxic therapy could be a new, practical, and useful therapeutic modality for obesity and obesity-related comorbidities.</P>
Park, Jong-il,Lee, Jisu,Kwon, Ju-Lee,Park, Hong-Bum,Lee, Su-Yel,Kim, Ji-Yeon,Sung, Jaekye,Kim, Jin Man,Song, Kyu Sang,Kim, Kyung-Hee Neoplasia Press 2016 Translational oncology Vol.9 No.1
<P>The aim of this study was to form a scaffold-free coculture spheroid model of colonic adenocarcinoma cells (CACs) and normal colonic fibroblasts (NCFs) and to use the spheroids to investigate the role of NCFs in the tumorigenicity of CACs in nude mice. We analysed three-dimensional (3D) scaffold-free coculture spheroids of CACs and NCFs. CAC Matrigel invasion assays and tumorigenicity assays in nude mice were performed to examine the effect of NCFs on CAC invasive behaviour and tumorigenicity in 3D spheroids. We investigated the expression pattern of fibroblast activation protein-α (FAP-α) by immunohistochemical staining. CAC monocultures did not form densely-packed 3D spheroids, whereas cocultured CACs and NCFs formed 3D spheroids. The 3D coculture spheroids seeded on a Matrigel extracellular matrix showed higher CAC invasiveness compared to CACs alone or CACs and NCFs in suspension. 3D spheroids injected into nude mice generated more and faster-growing tumors compared to CACs alone or mixed suspensions consisting of CACs and NCFs. FAP-α was expressed in NCFs-CACs cocultures and xenograft tumors, whereas monocultures of NCFs or CACs were negative for FAP-α expression. Our findings provide evidence that the interaction between CACs and NCFs is essential for the tumorigenicity of cancer cells as well as for tumor propagation.</P>