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Wang, Yong,He, Jianzhong,Yang, Wenxuan,Muhantay, Gemenggul,Chen, Ying,Xing, Jinming,Liu, Jianzhu Asian Australasian Association of Animal Productio 2015 Animal Bioscience Vol.28 No.10
This study aims to determine the polymorphism and mRNA expression pattern of the heart-type fatty acid-binding protein (H-FABP) gene and their association with intramuscular fat (IMF) content in the breast and leg muscles of Baicheng oil chicken (BOC). A total of 720 chickens, including 240 black Baicheng oil chicken (BBOC), 240 silky Baicheng oil chicken (SBOC), and 240 white Baicheng oil chicken (WBOC) were raised. Three genotypes of H-FABP gene second extron following AA, AB, and BB were detected by polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) strategy. The G939A site created AA genotype and G956A site created BB genotype. The content of IMF in AA genotype in breast muscle of BBOC was significantly higher than that of AB (p = 0.0176) and the genotype in leg muscle of WBOC was significantly higher than that of AB (p = 0.0145). The G939A site could be taken as genetic marker for higher IMF content selecting for breast muscle of BBOC and leg muscle of WBOC. The relative mRNA expression of H-FABP was measured by real-time PCR at 30, 60, 90, and 120 d. The IMF content significantly increased with age in both muscles. The mRNA expression level of H-FABP significantly decreased with age in both muscles of the three types of chickens. Moreover, a significant negative correlation between H-FABP abundance and IMF content in the leg muscles of WBOC (p = 0.035) was observed. The mRNA expression of H-FABP negatively correlated with the IMF content in both breast and leg muscles of BOC sat slaughter time.
Yong Wang,Jianzhong He,Wenxuan Yang,Gemenggul Muhantay,Ying Chen,Jinming Xing,Jianzhu Liu 아세아·태평양축산학회 2015 Animal Bioscience Vol.28 No.10
This study aims to determine the polymorphism and mRNA expression pattern of the heart-type fatty acid-binding protein (H-FABP) gene and their association with intramuscular fat (IMF) content in the breast and leg muscles of Baicheng oil chicken (BOC). A total of 720 chickens, including 240 black Baicheng oil chicken (BBOC), 240 silky Baicheng oil chicken (SBOC), and 240 white Baicheng oil chicken (WBOC) were raised. Three genotypes of H-FABP gene second extron following AA, AB, and BB were detected by polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) strategy. The G939A site created AA genotype and G956A site created BB genotype. The content of IMF in AA genotype in breast muscle of BBOC was significantly higher than that of AB (p = 0.0176) and the genotype in leg muscle of WBOC was significantly higher than that of AB (p = 0.0145). The G939A site could be taken as genetic marker for higher IMF content selecting for breast muscle of BBOC and leg muscle of WBOC. The relative mRNA expression of H-FABP was measured by real-time PCR at 30, 60, 90, and 120 d. The IMF content significantly increased with age in both muscles. The mRNA expression level of H-FABP significantly decreased with age in both muscles of the three types of chickens. Moreover, a significant negative correlation between H-FABP abundance and IMF content in the leg muscles of WBOC (p = 0.035) was observed. The mRNA expression of H-FABP negatively correlated with the IMF content in both breast and leg muscles of BOC sat slaughter time.
The Research of Intelligent Irrigation Control System of Rice in Cold Region
Na Wang,Jinming Liu 보안공학연구지원센터 2015 International Journal of Smart Home Vol.9 No.9
In traditional way of rice irrigation in the Heilongjiang region,there are many problems such as huge consumption of water, complex wiring and cold water harm. To improve these problems, the intelligent irrigation control system based on PLC and configuration software is designed. The data acquisition and monitoring of water level and water temperature and other parameters are carried through the wireless transmission with PLC as the core, to decide whether irrigation and drainage. In order to solve the problem of cold water harm of rice,the water of Sun pool is heated by solar heating system. The use of solar panels and batteries for power supply of each part, solve the problem of large power loss. The application proves that the system has a positive effect on water—saving and improving the quality and yield of rice, improving the intelligent of irrigation system.
Hui Chen,Jinming Liu,Bin Wang,Yongjun Li 대한약학회 2020 Archives of Pharmacal Research Vol.43 No.12
Long non-coding RNAs (lncRNAs) are boundup with the regulation of various diseases. Here, we probedinto the effect of lncRNA colorectal neoplasia differentiallyexpressed (CRNDE) on heart failure (HF). The pathologicalalterations and cell apoptosis of heart tissues were observedby hematoxylin–eosin and TUNEL staining. The viabilityor apoptosis of mouse myocardial cells HL-1 was tested byXTT or flow cytometry. The interaction between lncRNACRNDE and poly-ADP-ribose polymerase 1 (PARP-1) wasverified by RNA immunoprecipitation and RNA pull-down. The stability of the PARP-1 protein and the acetylation levelof high mobility group box-1 (HMGB1) were determined bycycloheximide-chase and immunoprecipitation, respectively. LncRNA CRNDE expression was decreased in HF micetissues and doxorubicin (Dox)-treated HL-1 cells, whereasPARP-1 and HMGB1 were increased. The overexpressionof lncRNA CRNDE restrained HL-1 cell apoptosis inducedby Dox. Moreover, the interaction between CRNDE andPARP-1 was corroborated, CRNDE negatively regulatedPARP-1 expression, and the overexpression of CRNDEreduced PARP-1 protein stability. In HL-1 cells, PARP-1positively regulated the acetylation level and cytoplasmtranslocation of HMGB1. CRNDE restrained Dox-inducedapoptosis in mouse myocardial cells via the PARP-1/HMGB1 pathway.
Babeisa duncani infection alters gut microbiota profile in hamsters
Shangdi Zhang,Jinming Wang,Xiaoyun Li,Yanbo Wang,Yueli Nian,Chongge You,Dekui Zhang,Guiquan Guan 대한기생충학ㆍ열대의학회 2023 The Korean Journal of Parasitology Vol.61 No.1
The genus Babesia includes parasites that can induce human and animal babesiosis, which are common in tropical and subtropical regions of the world. The gut microbiota has not been examined in hamsters infected by Babesia duncani. Red blood cells infected with B. duncani were injected into hamsters through intraperitoneal route. To evaluate the changes in gut microbiota, DNAs were extracted from small intestinal contents, acquired from hamsters during disease development. Then, the V4 region of the 16S rRNA gene of bacteria was sequenced using the Illumina sequencing platform. Gut microbiota alternation and composition were assessed according to the sequencing data, which were clustered with >97.0% sequence similarity to create amplicon sequence variants (ASVs). Bacteroidetes and Firmicutes were made up of the major components of the gut microbiota in all samples. The abundance of Bacteroidetes elevated after B. duncani infection than the B. duncani-free group, while Firmicutes and Desulfobacterota declined. Alpha diversity analysis demonstrated that the shown ASVs were substantially decreased in the highest parasitemia group than B. duncani-free and lower parasitemia groups. Potential biomarkers were discovered by Linear discriminant analysis Effect Size (LEfSe) analysis, which demonstrated that several bacterial families (including Muribaculaceae, Desulfovibrionaceae, Oscillospiraceae, Helicobacteraceae, Clostridia UGG014, Desulfovibrionaceae, and Lachnospiraceae) were potential biomarkers in B. duncani-infected hamsters. This research demonstrated that B. duncani infectious can modify the gut microbiota of hamsters.
AUTOMORPHISMS OF THE ZERO-DIVISOR GRAPH OVER 2 × 2 MATRICES
Ma, Xiaobin,Wang, Dengyin,Zhou, Jinming Korean Mathematical Society 2016 대한수학회지 Vol.53 No.3
The zero-divisor graph of a noncommutative ring R, denoted by ${\Gamma}(R)$, is a graph whose vertices are nonzero zero-divisors of R, and there is a directed edge from a vertex x to a distinct vertex y if and only if xy = 0. Let $R=M_2(F_q)$ be the $2{\times}2$ matrix ring over a finite field $F_q$. In this article, we investigate the automorphism group of ${\Gamma}(R)$.
Automorphisms of the zero-divisor graph over $2\times 2$ matrices
Xiaobin Ma,Dengyin Wang,Jinming Zhou 대한수학회 2016 대한수학회지 Vol.53 No.3
The zero-divisor graph of a noncommutative ring $R$, denoted by $\Gamma(R)$, is a graph whose vertices are nonzero zero-divisors of $R$, and there is a directed edge from a vertex $x$ to a distinct vertex $y$ if and only if $xy=0$. Let $R=M_{2}(F_q)$ be the $2\times 2$ matrix ring over a finite field $F_q$. In this article, we investigate the automorphism group of $\Gamma(R)$.
Design and Application of Self-Lifting & Slewing Multi-Cranes Platform
Kun Zhang,Kaiqiang Wang,Bo Chen,Qing Sun,Hui Yang,Xin Ba,Jinming Zhao Council on Tall Building and Urban Habitat Korea 2022 International journal of high-rise buildings Vol.11 No.2
Tower crane is of great importance in the construction of high rise buildings. A self-lifting & slewing multi-cranes platform (referred to as crane slewing platform) was developed to optimize the configuration of tower cranes, as well as solve the problems of cooperative operation conflict between multiple cranes and other construction equipment and their respective climbing and occupying of construction period. The design and test of the slewing platform was introduced. By applying the slewing platform in the construction of Chengdu Greenland Center super high rise building project, some key technologies such as the configuration of cranes, the installation, construction and lifting of the slewing platform are implemented and validated. Up to now, the slewing platform has been safely lifted up 98 times in Chengdu Greenland Center project construction, and achieved good social and economic benefits.