Rapid quantification of viable spore used in healing concrete cracks by a simple spectrophotometric method

Jinlong Zhang,Jingkun Lu,Bing Liu,Qiuyue Liu,Fan Jin,Miaojun Zhang,Yerong Liu,Yujun Song,Chenhui Dong,Wanyi Zhang,Ningxu Han,Xu Deng,Feng Xing 한양대학교 세라믹연구소 2019 Journal of Ceramic Processing Research Vol.20 No.S1

Quantification of viable spores is a time taking task due to the lack of rapid, efficient and accurate methods. This studypresented a simple spectrophotometric method for the detection of viable spores based on spore’s property of losing refractivityduring the germination process. By comparison of the results obtained by both spectrophotometric method and colonycounting method, a good linear correlation (R2 = 0.99) was achieved between viable spore concentration and OD loss underappropriate conditions. To avoid interference from ungerminable spores and vegetative cells, a turbidity complementationstrategy of keeping the initial concentration of spore suspensions at the same and relatively lower level was required. Thecalibration equation developed could be used to predict the viable spore yield produced in a series of fermentation experiments. The experimental results proved that this novel spectrophotometric method was sensitive, rapid, and easy to performcompared to conventional colony counting method.

Novel Mutations in CYP51B from Penicillium digitatum Involved in Prochloraz Resistance

Jinlong Wang,Jinhui Yu,Jing Liu,Yongze Yuan,Na Li,Muqing He,Ting Qi,Geng Hui,Li Xiong,Deli Liu 한국미생물학회 2014 The journal of microbiology Vol.52 No.9

Green mold caused by Penicillium digitatum is one of themost serious postharvest diseases of citrus fruit, and it isubiquitous in all citrus growing regions in the world. Sterol14α-demethylase (CYP51) is one of the key enzymes of sterolbiosynthesis in the biological kingdom and a prime targetof antifungal drugs. Mutations in CYP51s have been foundto be correlated with resistance to azole fungicides in manyfungal species. To investigate the mechanism of resistance toprochloraz (PRC) in P. digitatum, the PRC sensitivity wasdetermined in vitro in this study to assess the sensitivity of78 P. digitatum isolates collected in Hubei province. Theresults showed that 25 isolates were prochloraz-resistant(PRC-R), including six high-resistant (HR) strains, twelvemedium-resistant (MR) and seven low-resistant (LR) strains. A sequence analysis showed no consistent point mutationsof PdCYP51A in the PRC-R strains, but four substitutionsof CYP51B were found, Q309H in LR strains, Y136H andQ309H in HR strains, and G459S and F506I in MR strains,which corresponded to the four sensitivity levels. Based onthe sequence alignment analysis and homology modelingfollowed by the molecular docking of the PdCYP51B protein,the potential correlation between the mutations and PRC resistance is proposed.

Evaluation of sex attractants for monitoring of Illiberis ulmivora Graeser (Lepidoptera: Zygaenidae: Procridinae): Height, distance, placement, climatic conditions

Liu Hongxia,Dou Rui,Wang Jifei,Zhu Yachao,Li Jingyao,Liu Jinlong,Wang Yang,Zhang Jintong,Jing Xiaoyuan 한국곤충학회 2022 Entomological Research Vol.52 No.2

The phytophagous caterpillar Illiberis ulmivora is a serious pest of Ulmus glaucescens Franch (Ulmaceae, Ulmus). In order to test potent sex attractant, field trails of synthetic candidate attractants were conducted in China. In this study, baits with ternary blends, (2S)-butyl (7Z)-dodecenoate, (2S)-butyl (9Z)-tetradecenoate, and (2S)-butyl (5Z)-decenoate (8:4:1 ratio) resulted in a higher number of male moths capture than the rest of the baits that were offered. Traps positioned at a height of 1.2 m attracted more males than those installed at heights of 2.2 m and 3.2 m. The slope aspect, temperature and humility had a significant effect on the trap catches. More catches occurred in traps placed on semi-shady and semi-sunny slopes than those placed on sunny and shady slopes. The number of pheromones in the septa decreased with age. The residual amounts of pheromones in the lures combined with 2,6-di-tert - butyl-p-cresol (BHT) were higher than those in the control. However, there were no differences in the male captures between components and components combined with BHT during the first 8 d. These findings on monitoring and trap positions can be used to predict the occurrence and population dynamics of I. ulmivora, to develop ecological management of I. ulmivora.

Classroom Roll-Call System Based on ResNet Networks

Jinlong Zhu,Fanhua Yu,Guangjie Liu,Mingyu Sun,Dong Zhao,Qingtian Geng,Jinbo Su 한국정보처리학회 2020 Journal of information processing systems Vol.16 No.5

A convolution neural networks (CNNs) has demonstrated outstanding performance compared to otheralgorithms in the field of face recognition. Regarding the over-fitting problem of CNN, researchers haveproposed a residual network to ease the training for recognition accuracy improvement. In this study, a novelface recognition model based on game theory for call-over in the classroom was proposed. In the proposedscheme, an image with multiple faces was used as input, and the residual network identified each face with aconfidence score to form a list of student identities. Face tracking of the same identity or low confidence weredetermined to be the optimisation objective, with the game participants set formed from the student identitylist. Game theory optimises the authentication strategy according to the confidence value and identity set toimprove recognition accuracy. We observed that there exists an optimal mapping relation between face andidentity to avoid multiple faces associated with one identity in the proposed scheme and that the proposedgame-based scheme can reduce the error rate, as compared to the existing schemes with deeper neural network.

Sa-tree culture and tradition in the Central Plain of China

이정호,Jinlong Liu 한국산림과학회 2014 Forest Science And Technology Vol.10 No.3

Sa-tree sylvanism has led to the Earth Ritual and Sajik Altar tradition. The Sa-institution had been in the Yu and Xiaancient states of the later Neolithic and early Bronze-Age, and in the Shang and Zhou kingdoms of the Bronze Age. Lu andSong, the two feudal states of the Zhou kingdom, had two types of Sa-precincts. Pine, cypress, chestnut, catalpa, scholartree, and elms were Sa-tree species. Ancient Sa-precincts functioned as a place of the Earth Ritual, juridical court, militarygatherings, and communitarian public meetings. One of the highest six ministers (Earth official) who was in charge of Sainstitutionwas appointed with the practical officials who had to take care of the Sa-precinct, mountains, forests, rivers,marshes, and hunting. Sa-institution was practiced for all the people, not only kings and dukes, but also for commonpeople. Inhabitants at villages in Zhou, Qin and Han were grouped into a Sa unit, which has also been used as anadministrative unit. The ancient Sa-tree culture has common ground with the sylvanistic culture of current minoritiesin mountainous areas of China. Sa-tree culture and tradition can be brought to modern consciousness, and its implicationsin culture, forestry and social organization should be harnessed by members of modern society in Northeast Asia.

Route Optimization Algorithm Based on Game Theory for Tourism Routes at Pseudo-Imperial Palace

( Guangjie Liu ),( Jinlong Zhu ),( Qiucheng Sun ),( Jiaze Hu ),( Hao Yu ) 한국정보처리학회 2021 Journal of information processing systems Vol.17 No.5

With improvements in living conditions, an increasing number of people are choosing to spend their time traveling. Comfortable tour routes are affected by the season, time, and other local factors. In this paper, the influencing factors and principles of scenic spots are analyzed, a model used to find the available routes is built, and a multi-route choice model based on a game theory utilizing a path recommendation weight is developed. A Monte Carlo analysis of a tourist route subjected to fixed access point conditions is applied to account for uncertainties such as the season, start time, end time, stay time, number of scenic spots, destination, and start point. We use the Dijkstra method to obtain multiple path plans and calculate the path evaluation score using the Monte Carlo method. Finally, according to the user preference in the input path, game theory generates path ordering for user choice. The proposed approach achieves a state-of-the-art performance at the pseudo-imperial palace. Compared with other methods, the proposed method can avoid congestion and reduce the time cost.

Experimental study of internal solitary wave loads on the semi-submersible platform

Zhang Jingjing,Liu Yi,Chen Ke,You Yunxiang,Duan Jinlong 대한조선학회 2021 International Journal of Naval Architecture and Oc Vol.13 No.1

A prediction method, based on the Morison equation as well as Froude-Krylov formula, is presented to simulate the loads acting on the columns and caissons of the semi-submersible platform induced by Internal SolitaryWave (ISW) respectively. Combined with the experimental results, empirical formulas of the drag and inertia coefficients in Morison equation can be determined as a function of the Keulegan eCarpenter (KC) number, Reynolds number (Re) and upper layer depth h1=h respectively. The experimental and calculated results are compared. And a good agreement is observed, which proves that the present prediction method can be used for analyzing the ISW-forces on the semi-submersible platform. Moreover, the results also demonstrate the layer thickness ratio has a significant effect upon the maximum horizontal forces on the columns and caissons, but both minimum horizontal and vertical forces are scarcely affected. In addition, the incoming wave directions may also contribute greatly to the values of horizontal forces exerted on the caissons, which can be ignored in the vertical force analysis.

Characterization of genes coding for galacturonosyltransferaselike (GATL) proteins in rice

Shaobo Li,Jinlong Liu,Mansi Luo,Xin Yan,Chao Yu 한국유전학회 2016 Genes & Genomics Vol.38 No.10

In the present study, seven galacturonosyltransferase- like (GATL) genes (OsGATLs) in rice (Oryza sativa L.) were genome-widely identified and the chromosomal locations and the gene structures of which were characterized. Under normal condition, OsGATL2 and OsGATL3 are highly expressed in root, while OsGATL4 is highly expressed in stem and leaf. Many cis-elements related to stress response and plant hormone were found in the promoter sequence of each OsGATL. The expression patterns of these OsGATL genes under treatment with abscisic acid (ABA), drought and low temperature were assessed by qRT-PCR. The expression levels of most OsGATLs significantly increased following the treatments with drought or low temperature. In addition, physicochemical properties of OsGATLs and phylogenetic analysis with GATL from rice and several other species were performed. 3D structures and protein–protein interaction (PPI) network of OsGATLs were further predicted by Swiss-model and STRING 9.0 database, respectively. The identification and bioinformatic analysis of GATL family in rice could provide reference data for further study on their biological functions, especially in the responsiveness to hormones and stress signaling.

Hyper-heuristic algorithm for traffic flow-based vehicle routing problem with simultaneous delivery and pickup

Wang Zhengwei,Liu Jinlong,Zhang Jingling 한국CDE학회 2023 Journal of computational design and engineering Vol.10 No.6

To address the realistic problem of seriously reducing distribution efficiency and increasing distribution cost caused by road traffic congestion, this paper constructs a time-dependent speed describing vehicle travel speed and road traffic flow by simulating the change of urban traffic flow, to establish a vehicle route problem model considering traffic flow with distribution cost and customer satisfaction as optimization objectives. To solve this problem, a hyper-heuristic algorithm based on tabu search is designed in this paper, in which the underlying search operator is selected more efficiently by a high-level heuristic strategy. In addition, the correctness of the model and the effectiveness of the algorithm are verified by conducting simulation experiments on several benchmark sets. Experiment results are shown as the travel speed of the vehicle increases, the average customer satisfaction in lc1-type instances increases to 0.94. And the impact of urban traffic changes on logistics costs and customer satisfaction is further analyzed.

SNPs in the coding region of bovine MGAT2 gene are associated with body weight and weight gain

Lian Qu,Mingjuan Yang,Jinlong Zhu,Junxia Liu,Mijie Li,Liangzhi Zhang,Xianyong Lan,Chuzhao Lei,Chunlei Zhang,Hong Chen 한국유전학회 2011 Genes & Genomics Vol.33 No.3

Monoacylglycerol acyltransferase 2 (MGAT2), as a candidate gene for quantitative traits, relates to dietary fat uptake, lipids synthesis and storage, which plays a major role in the absorption of dietary fat by catalyzing the resynthesis of triacylglycerol in enterocytes. In this study, based on DNA pool sequencing and PCR‐RFLP methods, polymorphisms of the MGAT2 gene were detected in 1145 Chinese indigenous cattle. The results revealed two novel mutations located on exon 1and exon 5 (NM_001099136.1:m.84G>T and 756A>G). Hence, we described the HaeIII forced PCR–RFLP method in exon1 and a MluI PCR–RFLP method in exon5 to detect them. In addition, the associations of these polymorphisms with growth traits were evaluated in Nanyang cattle. The results showed that only HaeIII locus was associated with body weight and average daily gain aged 6 months, and individuals with genotype TT showed significantly higher body weight and average daily gain than those with genotype GG.