Odorant Binding Protein-based Biosensor for Real-time Assessment of Salmonella Contamination in Packaged Food

Jinkyung KANG,jong Hyun LIM,Manki SON,Daesan KIM,Seunghun HONG,Tai Hyun PARK 한국생물공학회 2014 한국생물공학회 학술대회 Vol.2014 No.10

Single atomic spin sensing of magnetic interactions in a tunnel junction

Jinkyung Kim,Won-jun Jang,Thi Hong Bui,Deung-Jang Choi,Christoph Wolf,Fernando Delgado,Yi Chen,Denis Krylov,Soonhyeong Lee,Sangwon Yoon,Christopher P. Lutz,Andreas J. Heinrich,Yujeong Bae 한국자기학회 2021 한국자기학회 학술연구발표회 논문개요집 Vol.31 No.1

Single spins are widely regarded as a leading candidate for realizing next-generation quantum devices for sensing and quantum information processing. Detection and coherent control of single spins require to localize single spins and characterize its magnetic surroundings. Scanning tunneling microscopy in combination with electron spin resonance (ESR-STM) technique [1] enables a direct access to the quantum states of single magnetic atoms or molecules on surfaces. Using ESR-STM, we investigated spin resonance of hydrogenated Ti (TiH) atoms adsorbed on bridge binding site of MgO in a two-dimensional vector magnetic field. Here, the spin 1/2 TiH atom with no magnetic anisotropy was employed as a probe of magnetic environments at the tunnel junction. We found both ESR frequency and amplitude change as a function of the angle of vector magnetic fields. The resonance frequency varied by different vector magnetic fields indicates an anisotropy of the g-factor, resulting from the variation of angular momentum contributions due to the crystal fields. We developed a stereoscopic way to unravel the g-factor along the three principal axes. Moreover, ESR amplitude dependence on the direction of magnetic fields provides the further understanding of ESR mechanisms, which results from two factors, tunneling magnetoresistance (TMR) effect at the spin-polarized STM junction and the transverse magnetic field to drive ESR. Our results will enable to predict ESR active spin centers on different substrates as well as in other quantum-nanoscience platforms.

Insulin Resistance and Its Association with Metabolic Syndrome in Korean Children

Cho, Jinkyung,Hong, Haeryun,Park, Soohyun,Kim, Shinuk,Kang, Hyunsik Hindawi 2017 BioMed research international Vol.2017 No.-

<P><B>Background</B></P><P> This study investigated the association between insulin resistance (IR) and metabolic syndrome (MetS) in children.</P><P><B> Methods</B></P><P> A cross-sectional study involving 1036 healthy children aged between 7 and 13 years was conducted. Homeostasis model assessment of insulin resistance (HOMA-IR) was calculated as an index of IR. Participants were classified according to the HOMA-IR quartiles.</P><P><B> Results</B></P><P> Incremental, linear trends were found in age (<I>p</I> < 0.001), body mass index (BMI) (<I>p</I> < 0.001), body fat (<I>p</I> < 0.001), waist circumference (<I>p</I> < 0.001), resting blood pressures (BP) (<I>p</I> < 0.001), triglycerides (TG) (<I>p</I> < 0.001), total cholesterol (TC) (<I>p</I> < 0.001), high density lipoprotein-cholesterol (HDL-C) (<I>p</I> < 0.001), FBG (<I>p</I> < 0.001), and insulin (<0.001) according to incremental HOMA-IR categories (from the 1st to 4th quartile). Compared with children in the 1st HOMA-IR quartile, children in the 4th HOMA-IR quartile had significantly higher odd ratios (ORs) of abnormalities in systolic (<I>p</I> = 0.051) and diastolic BP (<I>p</I> = 0.005), FBG (<I>p</I> < 0.001), TG (<I>p</I> < 0.001), TC (<I>p</I> = 0.016), and HDL-C (<I>p</I> = 0.006) even after adjustments for age, gender, BMI, and body fat percentage. Children in the 3rd HOMA-IR quartile had significant abnormalities in FBG (<I>p</I> < 0.001), TG (<I>p</I> = 0.001), and HDL-C (<I>p</I> = 0.010) even after adjustments for the covariates.</P><P><B> Conclusion</B></P><P> The current findings suggest that IR is significantly associated with the clustering of MetS risk factors in children in Korea. </P>

운동선수의 비타민D에 대한 최신지견

조진경 ( Jinkyung Cho ),박수현 ( Soo-hyun Park ),송홍선 ( Hong-sun Song ) 한국운동생리학회 2021 운동과학 Vol.30 No.3

PURPOSE: Vitamin D plays important roles in calcium homeostasis and bone metabolism. Since vitamin D receptors (VDRs) are located in a variety of organs, including skeletal muscle, vitamin D has potentially widespread effects. The purpose of this review was to summarize the current understanding of the effects of vitamin D on muscle function and exercise performance in athletes. METHODS: In this narrative review, we summarized previous studies by searching the literature in the PubMed, Google Scholar, and Science Direct databases. RESULTS: Vitamin D has been shown to regulate multiple actions in skeletal muscle tissue, such as myocyte proliferation and growth via genomic and non-genomic molecular pathways. Higher levels of vitamin D are associated with improved skeletal muscle function and exercise performance. Moreover, in some studies, vitamin D supplementation has beneficial effects on muscle strength in athletes, especially those who are vitamin D-deficient. CONCLUSIONS: Vitamin D appears to have beneficial effects on muscle and exercise performance in athletes. However, more studies are needed to clarify the action and dosage of vitamin D in athletes.

Early Growth Response 1-Dependent Downregulation of Matrix Metalloproteinase 9 and Mouse Double Minute 2 Attenuates Head and Neck Squamous Cell Carcinoma Metastasis

Kim, Jinkyung,Kang, Sung-Min,Oh, Su Young,Kang, Soo Hyun,Lee, Inhan,Hwang, Jae Chan,Lee, Heon-Jin,Choi, So-Young,Hong, Su-Hyung S. Karger AG 2018 CELLULAR PHYSIOLOGY AND BIOCHEMISTRY Vol.50 No.5

<P><B><I>Background/Aims:</I></B> The functional relevance of early growth response-1 (EGR1) on cancer invasion remains controversial. The effect of EGR1 on the expression of MMP9, which is important for HNSCC invasion, is still disputed. There is no previous data showing the effect of EGR1 on mouse double minute 2 (MDM2), an enhancer of matrix metalloproteinase 9 (MMP9) expression. Our aim is to clarify the negative correlation between EGR1 expression and head and neck squamous cell carcinoma (HNSCC) metastasis. <B><I>Methods:</I></B> EGR1 mRNA and protein expressions were compared in normal and HNSCC tissues using The Cancer Genome Atlas (TCGA) dataset analysis or immunohistochemistry (IHC), respectively. <I>In vitro</I> cell invasion was evaluated Matrigel invasion assay. EGR1-dependent inhibition of MDM2 transcription was assessed by promoter–luciferase assay and chromatin immunoprecipitation (ChIP). <B><I>Results:</I></B> TCGA data showed that EGR1 mRNA levels are significantly higher in normal oral tissues as compared with HNSCC tumor tissues (adjusted P = 1.64x10<SUP>-16</SUP>). In addition, nonmetastatic HNSCC tissues showed significantly higher EGR1 mRNA levels as compared with metastatic tissues (adjusted P = 0.023). IHC analysis showed that primary tumor tissues expressed significantly higher levels of nuclear EGR1 compared with paired metastatic lymph node tissues (P < 0.05). EGR1 overexpression downregulated MMP9 and MDM2 protein expression. Consistent with these observations, TCGA data analysis found significantly fewer metastatic patients among a subgroup of population presenting higher EGR1 expressions with lower MMP9 and/or MDM2. <B><I>Conclusion:</I></B> Our data suggests that EGR1 prevents HNSCC metastasis through downregulation of MMP9 and MDM2. EGR1 might be a potential candidate to attenuate HNSCC metastasis.</P>

대전지역 성인의 커뮤니티 키친에 대한 인식 및 요구도

김진경(Kim, Jinkyung),이다혜(Lee, Dahye),육홍선(Yook, Hong-Sun),전민선(Jeon, Min-Sun) 한국생활과학회 2022 한국생활과학회 학술대회 Vol.2022 No.5

저온기 송풍덕트 적용 저절위 착과와 재사용 배지 참외의 초기생육 특성

홍영신 ( Youngsin Hong ),박소현 ( Sohyun Park ),장세훈 ( Saehoon Jang ),윤성욱 ( Sungwook Yun ),권진경 ( Jinkyung Kwon ),장재경 ( Jaekyung Jang ),문종필 ( Jongpil Moon ),이시영 ( Siyoung Lee ),이상규 ( Sanggyu Lee ),배효준 ( Hyoju 한국농업기계학회 2023 한국농업기계학회 학술발표논문집 Vol.28 No.1

참외는 재배 시 노동강도가 높은 포복재배로 작업자의 근골격계 질환 발생 위험이 커서 이를 줄일 수 있는 수경ㆍ수직재배가 시도되고 있다. 본 연구는 저온기 송풍덕트 난방으로 수직ㆍ수경재배 참외의 저절위 착과와 재활용배지에 대한 초기생육특성을 분석하여 새로운 재배법 개발에 활용하고자 하였다. 참외의 과실 착과는 아들덩굴 5마디 이하에서 발생하는 손자덩굴을 모두 제거하는 처리가 일반적이나, 아들덩굴 1마디에서 발생하는 손자덩굴에 착과 처리하는 저절위착과 하였다. 수직재배 시 줄기 유인을 아래에서 위로 유인하는 상향처리, 위에서 아래로 유인하는 하향처리로 하였다. 재사용배지 적용을 위하여 각 처리구에 재사용배지와 일반배지를 배치하였다. 저온기 난방을 위해 재배베드 아래에 두께 0.15mm, 길이 30m, 지름 30cm, 천공열 1줄, 천공지름 1.6과 1.8cm로 각 처리구에 설치하였다. 길이 30m 덕트의 1m, 15m, 29m부근에 온ㆍ습도센서를 24개 설치하여 비교하였다. 처리구는 일반배지(상향), 일반배지(하향), 재사용배지(상향), 재사용배지(하향) 총 4처리구였다. 양액은 야마자키 멜론 배양액을 사용하여 양액EC 0.8-2.0dSㆍm^-1, pH 6.0으로 관리하였다. 양액공급은 0.8-2.5L/일/주로 생육단계에 맞게 공급하였다. 온실 내부 일평균온도는 16-21°C, 상대습도는 53-86%, CO₂농도는 429-534ppm, 일적산광량은 0.13-1.04MJㆍm^-2였다. 초기생육조사는 정식후 7주가 되는 2023년 1월 9일에 아들줄기 12마디 적심전에 실시하였다. 송풍덕트 천공크기에 대한 내부 온도 차이는 1,6cm가 1.8cm보다 상향은 -0.88-0.34°C(1M), -0.88-0.34°C(15M), -0.57-0.29°C(29M)로 높게 나타났고, 하향은 -0.93-0.36°C(1M), -1.66-0.96°C(15M), -0.47-0.29°C(29M)로 높게 나타났다. 따라서, 송풍덕트 천공넓이는 1.6cm가 1.8cm보다 난방 효과가 있은 것으로 나타났다. 재사용배지(하향)과 일반배지(하향)처리구의 초장이 146cm로 재사용배지(상향)과 일반 배지(상향)보다 7-22cm 더 컸다. 마디수와 아들줄기의 엽수는 하향재배가 상향재배보다 마디수는 1-2개와 엽수 4-6개 더 많았다. 엽록소(SPAD)는 하향재배가 상향재배보다 39.3-43.2로 높았다(p<0.0001). 광합성은 하향재배가 14.3 μmolㆍm^-2ㆍs^-1로 상향재배 11.8 μmolㆍm^-2ㆍs^-1보다 높게 나타났다(p<0.0001). 재사용배지와 일반배지에 대한 유의성은 없었으며, 상향재배보다 하향재배가 초장, 엽폭, 절간장, 엽록소가 높게 나타났다. 그러나, 엽수는 일반배지가 재사용 배지보다 많게 나타났다. 따라서, 참외 생육은 배지에 상관없이 하향재배가 좋은 것으로 나타났다. 본연구결과는 참외의 저온기 수직ㆍ수경재배법 개발에 저절위 착과와 재사용배지에 활용할 수 있을 것으로 판단된다.

실어증 환자들을 위한 CILT 중재 효과에 대한 메타분석

홍새미(SaeMi Hong),강진경(JinKyung Kang),엄보라(Bora Eom),김영태(YoungTae Kim),성지은(JeeEun Sung),심현섭(HyunSub Sim),정필연(PilYeon Jeong) 한국언어청각임상학회 2016 Communication Sciences and Disorders Vol.21 No.1

Constraint-Induced Language Therapy (CILT) 중재는 집중적인 일정 동안 구어를 극대화하여 사용할 수 있게 구조화한 실어증 중재방법이다. 여러 연구들이 CILT 중재의 효과를 검증하였지만 상이한 연구결과가 혼재하였다. 이에 본 연구에서는 메타 분석을 통해 실어증 환자 대상으로 CILT 중재의 효과성을 보고한 개별 연구들의 결과를 종합하여 증거기반 실제를 구축하고자 하였다. 방법: 총 5개의 국내외 데이터베이스를 이용하여 실어증 환자를 대상으로 CILT 중재를 실시한 연구들 중 선정기준에 부합한 총 9편의 문헌을 수집하였다. 그리고 중재에 따른 언어검사 결과를 종합하여 언어의 하위 영역 및 실어증 발병기간에 따른 효과크기를 각각 살펴보았다. 결과: CILT 중재는 실어증 환자의 전반적인 언어능력을 비롯하여 청각적 이해력, 따라 말하기, 이름대기 능력 향상에 유의한 효과가 있는 것으로 나타났다. 또한 CILT 중재는 급성실어증과 만성실어증 환자의 전반적인 언어능력 및 모든 언어 하위 영역 향상에서도 유의한 효과가 있는 것으로 나타났다. 논의 및 결론: 본 연구는 CILT 중재의 종합적인 효과 검증을 통해 CILT 중재가 발병기간과 무관하게 실어증 환자의 전반적인 언어능력과 언어의 각 하위 영역을 향상시키는 데 효과적이라는 결과를 도출하였다. 이를 통해 CILT 중재에 대한 기존 연구들의 상반된 결과들을 통일하여 임상적 유용성을 제고하는 근거를 마련하였다. Objectives: Constraint-Induced Language Therapy (CILT) is an aphasia treatment program which comprises forced use of verbal language and massed practice. There are many stud¬ies about the efficacy of CILT on aphasia, but the results are controversial. Thus, the pur¬pose of the present meta-analysis study was to systematically evaluate the effects of CILT for aphasia and to establish the evidence-based practice of CILT. Methods: Data collection was performed from 5 databases (DBPIA, EBSCOhost, ProQuest, PubMed, RISS) which were about CILT for aphasia. Nine studies which met the inclusive criteria were entered into the meta-analysis. Effect sizes for each study outcome (standardized language test outcome) were calculated. Results: There were two main findings from the current study. First, the meta-analysis results indicated that CILT is an effective treatment method for improving both overall language ability and each sub-language area. Second, CILT was effective for both chronic aphasia and acute aphasia—overall and in each sub-language area. Conclu¬sion: The present study suggests the comprehensive effectiveness of CILT for aphasia in each sub-language area including auditory comprehension, repetition, and naming, and its treatment effectiveness for both chronic aphasia and acute aphasia regardless of post onset time. This research unifies the complicated results of previous studies and lays the foundation for clinical implications.

소결온도 변화에 따른 텅스텐 재료의 강도 특성

홍희성(Huiseong Hong),이상필(Sangpill Lee),이진경(Jinkyung Lee),이문희(Moonhee Lee),김성원(Sungwon Kim),서병석(Seobyeong Seok),이서희(Seohee Lee) 대한기계학회 2020 대한기계학회 춘추학술대회 Vol.2020 No.12

In this study, the strength characteristics and microstructure of tungsten materials were investigated. Tungsten materials were fabricated using the combination of solid state sintering process and applied pressure. Tungsten powder was sintered at the sintering temperature of 1700 ℃ or less. Tungsten materials showed good flexural strength at ths sintering temperature of 1500 ℃, accompanying the creation of fine grain and the micro pore. However, tungsten materials fabricated at the sintering temperature above 1600 ℃ showed coarsening of tungsten grains and reduce flexural strength.

Sarcopenia Is Associated with Cognitive Impairment and Depression in Elderly Korean Women

LEE, Inhwan,CHO, Jinkyung,HONG, Haeryun,JIN, Youngyun,KIM, Donghyun,KANG, Hyunsik Tehran University of Medical Sciences 2018 Iranian journal of public health Vol.47 No.3

<P><B>Background:</B></P><P>Sarcopenia has been implicated in the increased risk for cognitive impairment and depression associated with aging. In this cross-sectional study, we investigated the relationship of sarcopenia with cognitive impairment (MCI) and depression in a sample of 201 community-dwelling Korean women (mean age of 74.0±6.8 yr) between 2014 and 2015.</P><P><B>Methods:</B></P><P>The Korean version of mini-mental state examination and the center for epidemiologic studies depression scale was used to assess cognitive performance and depression, respectively. Grp strength was measured with a dynamometer. Demographics, body composition, education, alcohol consumption, and history of cardiovascular diseases were assessed as covariates.</P><P><B>Results:</B></P><P>Odds ratio (OR) and 95% confidence interval (95% CI) of MCI and depression were calculated according to sarcopenia status. Compared to non-sarcopenic, pre-sarcopenic and sarcopenic women had the ORs of 2.160 (95% CI of 0.840 – 5.554, <I>P</I>=0.030) and of 5.493 (95% CI of 1.854 – 16.270, <I>P</I>=0.002) for MCI. The OR of pre-sarcopenia for MCI remained significant (<I>P</I>=0.030) even after adjustments for age, body mass index (BMI), lean body mass, and education, while the OR of sarcopenia for MCI was no longer significant (<I>P</I>=0.084) when adjusted for the covariates. Compared to non-sarcopenic, pre-sarcopenic and sarcopenic women had the ORs of 3.750 (95% CI of 1.137 – 12.370, <I>P</I>=0.030) and of 4.687 (95% CI of 1.127–19.505, <I>P</I>=0.034) for depression. The ORs of pre-sarcopenia and sarcopenia for depression remained statistically significant (<I>P</I>=0.020 and <I>P</I>=0.042, respectively) even after adjusted for the covariates</P><P><B>Conclusion:</B></P><P>Sarcopenia was significantly associated with MCI and depression in otherwise healthy community dwelling elderly Korean women.</P>