Imperatorin Sustained-release Tablets: In Vitro and Pharmacokinetic Studies

Jingjing Pan,Wen Lu,Changhui Li,Sicen Wang,Langchong He 대한약학회 2010 Archives of Pharmacal Research Vol.33 No.8

We prepared and evaluated imperatorin (IMP) sustained-release tablets. IMP is an active compound in Angelica dahuricae, a Chinese herbal medicine. We used different polymers, such as hydroxypropyl methylcellulose (HPMC K4M, K15M, and K100M), carbopol 934P, sodium carboxymethyl cellulose (CMC-Na), and their combinations to prepare the matrix tablets and achieve the desired sustained release profile. The in vitro release profiles of these formulations were examined and fit to various kinetic release models. We also tested the effects of polymer combination ratios on the in vitro release rate. In vivo studies were performed for the optimized formulation in six beagle dogs, and pharmacokinetic parameters were compared with plain IMP tablets. IMP sustained-release tablets exhibited a more sustained plasma concentration than the plain tablets, with a relative bioavailability of 127.25%. The in vitro releases rates and in vivo absorption correlated for the initial 8 hours. These results demonstrate that the sustained-release tablet system can effectively control the release of IMP.

Using digital gene expression profile to detect representational difference of Chlamys farreri genes after laboratory exposure to persistent organic pollutants

Luqing Pan,Jiaying Li,Jingjing Miao 한국유전학회 2016 Genes & Genomics Vol.38 No.3

Chlamys farreri (C. farreri), as an economic species of marine bivalves, can tolerate manifold anthropogenic stressors including persistent organic pollutants (POPs) stress. A systematic study of transcriptome after POPs exposure may provide insights into the mechanism of acquired pollution tolerance. In this study, we compared C. farreri transcriptome by reanalyzing DGE (digital gene expression) data from previous study. Our results revealed a toxicant-dependent pattern of global transcriptional responses, with 108, 126 and 138 DEGs regulated by BaP, TBBPA and PAHs cocktail (mixed by benzo(a)pyrene, benz(a)anthracene, benzo(b)fluoranthene and chrysene with the ratio of 5:3:1:1) exposure, respectively. Among these DEGs, 219 were commonly regulated by all pollutants, whereas the minority of differences were conditionspecific. In addition, we performed the first hierarchical cluster analysis of the common transcripts throughout three types of contaminants stress ulteriorly, leading to new discoveries of genes’ association information with dynamical gene expression data of C. farreri, which goes beyond the focus on individuals. It revealed a cascade of gene expression patterns in the response of C. farreri to different environmental stresses and may be beneficial for further analysis of novel environmental metrics.

Experiment Investigation on Fluid Transportation Performance of Propellant Acquisition Vanes in Microgravity Environment

Baotang Zhuang,Yong Li,Xianwu Luo,Halin Pan,Jingjing Ji 한국유체기계학회 2014 International journal of fluid machinery and syste Vol.7 No.1

The propellant acquisition vane (PAV) is a key part of a vane type surface tension propellant management device (PMD), which can manage the propellant effectively. In the present paper, the fluid transportation behaviors for five PAVs with different sections were investigated by using microgravity drop tower test. Further, numerical simulation for the propellant flow in a PMD under microgravity condition was also carried out based on VOF model, and showed the similar flow pattern for PAVs to the experiment. It is noted that the section geometry of PAVs is one of the main factors affecting the fluid transportation behavior of PMD. PAVs with bottom length ratio of 5/6 and 1/2 have larger propellant transportation velocity. Based on the experiments, there were two stages during the process of propellant transportation under microgravity environment: liquid relocation and steady transportation stage. It is also recognized that there is a linear correlation between liquid transportation velocity and relative time's square root. Those results can not only provide a guideline for optimization of new vane type PMDs, but also are helpful for fluid control applications in space environment.

Study on Microbial Community Succession and Protein Hydrolysis of Donkey Meat during Refrigerated Storage Based on Illumina NOVA Sequencing Technology

Zixiang Wei,Ruidong Chu,Lanjie Li,Jingjing Zhang,Huachen Zhang,Xiaohong Pan,Yifan Dong,Guiqin Liu 한국축산식품학회 2021 한국축산식품학회지 Vol.41 No.4

In this study, the microbial community succession and the protein hydrolysis of donkey meat during refrigerated (4℃) storage were investigated. 16S rDNA sequencing method was used to analyze the bacteria community structure and succession in the level of genome. Meanwhile, the volatile base nitrogen (TVB-N) was measured to evaluate the degradation level of protein. After sorting out the sequencing results, 1,274,604 clean data were obtained, which were clustered into 2,064 into operational taxonomic units (OTUs), annotated to 32 phyla and 527 genus. With the prolonging of storage time, the composition of microorganism changed greatly. At the same time, the diversity and richness of microorganism decreased and then increased. During the whole storage period, Proteobacteria was the dominant phyla, and the Photobacterium, Pseudompnas, and Acinetobacter were the dominant genus. According to correlation analysis, it was found that the abundance of these dominant bacteria was significantly positively correlated with the variation of TVB-N. And Pseudomonas might play an important role in the production of TVB-N during refrigerated storage of donkey meat. The predicted metabolic pathways, based on PICRUSt analysis, indicated that amino metabolism in refrigerated donkey meat was the main metabolic pathways. This study provides insight into the process involved in refrigerated donkey meat spoilage, which provides a foundation for the development of antibacterial preservative for donkey meat.

Experiment Investigation on Fluid Transportation Performance of Propellant Acquisition Vanes in Microgravity Environment

Zhuang, Baotang,Li, Yong,Luo, Xianwu,Pan, Halin,Ji, Jingjing Korean Society for Fluid machinery 2014 International journal of fluid machinery and syste Vol.7 No.1

The propellant acquisition vane (PAV) is a key part of a vane type surface tension propellant management device (PMD), which can manage the propellant effectively. In the present paper, the fluid transportation behaviors for five PAVs with different sections were investigated by using microgravity drop tower test. Further, numerical simulation for the propellant flow in a PMD under microgravity condition was also carried out based on VOF model, and showed the similar flow pattern for PAVs to the experiment. It is noted that the section geometry of PAVs is one of the main factors affecting the fluid transportation behavior of PMD. PAVs with bottom length ratio of 5/6 and 1/2 have larger propellant transportation velocity. Based on the experiments, there were two stages during the process of propellant transportation under microgravity environment: liquid relocation and steady transportation stage. It is also recognized that there is a linear correlation between liquid transportation velocity and relative time's square root. Those results can not only provide a guideline for optimization of new vane type PMDs, but also are helpful for fluid control applications in space environment.

In Vitro and In Vivo Study on the Effect of Lysosome-associated Protein Transmembrane 4 Beta on the Progression of Breast Cancer

Deyou Tao,Junqing Liang,Yihong Pan,Yanting Zhou,Ying Feng,Lin Zhang,Jingjing Xu,Hui Wang,Ping He,Jie Yao,Yang Zhao,Qinjie Ning,Wen Wang,Wei Jiang,Jing Zheng,Xia Wu 한국유방암학회 2019 Journal of breast cancer Vol.22 No.3

Purpose: Although the effect of lysosome-associated protein transmembrane 4 beta (LAPTM4B) on the proliferation, migration, and invasion of breast cancer (BC) cells has already been studied, its specific role in BC progression is still elusive. Here, we evaluated the effect of different levels of LAPTM4B expression on the proliferation, invasion, adhesion, and tumor formation abilities of BC cells in vitro, as well as on breast tumor progression in vivo. Methods: We investigated the influence of LAPTM4B expression on MCF-7 cell proliferation, invasion, adhesion, and tube formation abilities in vitro through its overexpression or knockdown and on breast tumor progression in vivo. Results: Cell growth curves and colony formation assays showed that LAPTM4B promoted the proliferation of breast tumor cells. Cell cycle analysis results revealed that LAPTM4B promoted the entry of cells from the G1 into the S phase. Transwell invasion and cell extracellular matrix adhesion assays showed that LAPTM4B overexpression increased the invasion and adhesion capabilities of MCF-7 cells. More branches were observed in MCF-7 cells overexpressing LAPTM4B under an electron microscope. In comparison with LAPTM4B overexpression, LAPTM4B knockdown decreased the expression of vascular endothelial growth factor-A and significantly inhibited the vasculogenic tube formation ability of tumors. These results were also verified with western blot analysis. Conclusion: LAPTM4B promoted the proliferation of MCF-7 cells through the downregulation of p21 (WAF1/CIP1) and caspase-3, and induced cell invasion, adhesion, and angiogenesis through the upregulation of hypoxia-inducible factor 1 alpha, matrix metalloproteinase 2 (MMP2), and MMP9 expression. This specific role deems LAPTM4B as a potential therapeutic target for BC treatment.