급성 백혈병 환자에서 감염증의 양상 및 예후인자에 관한 연구 : 1988년∼1995년까지

정희진,김병수,신상원,김열홍,김우주,김민자,김준석,박승철,김권범 대한감염학회 1998 감염 Vol.30 No.3

목적 : 급성 백혈병의 경우 골수기능이 억제되고 항암화학요법에 따른 골수기능 저하로 감염의 위험이 증가하며 감염의 치료가 환자의 생존에 큰 영향을 미치게 된다. 이에 저자들은 1988년부터 1995년까지 고대 구로병원에 급성 백혈병으로 입원했던 환자들 중 입원 기간 내 감염이 있었던 경우를 대상으로 하여 감염의 위험인자 및 감염증의 예후를 후향적으로 분석함으로써 향후 급성 백혈병환자의 감염의 치료에 대한 지침을 얻고자 하였다. 방법 : 급성 백혈병으로 확진된 환자들 중 감염병에 이환된 경우를 대상으로 하여 각각을 미생물학적 확인감염, 임상적 확인감염, 그리고 설명 불가열로 구분하였다. 각각의 경우에서 감염 당시의 호중구 수, 호중구 감소 기간, 항암화학요법과의 연관성, 감염 병소, 배양된 검체 및 원인균, 선택적 소화관 살균법의 사용여부 및 골수회복 여부 등을 조사한 후 환자의 예후와 어떤 관계가 있는 지 조사하였다. 결과 : 연구 대상은 총 113예로 남녀비는 46:67 이었고 평균연령은 34±13세, 진단은 급성 골수성 백혈병 (AML)이 84예, 급성 림프구성 백혈병(ALL)이 29예였으며, 감염시 평균 호중구수는 663±1678/㎣이었고, 호중구 감소 기간은 평균 18±13일이었다. 감염양상은 항암화학요법과 연관된 감염이 84예로 대부분이었고 미생물학적 확인 감염이 40예로서 35%였으며, 폐렴과 원발성 패혈증이 각각 20예와 19예로 가장 많았고, 카테터 연관 감염이 7예로 나타났다. 배양된 균주는 E. coli가 10예(25%)로서 가장 많았으며, 전체적으로 E. coli, P. aeruginosa, K. pneumoniae 등의 그람음성균이 63%로 대부분을 차지하였으나 최근 그람양성구군 및 진균의 분리율이 증가하는 양상을 보였다. 감염증의 예후를 분석한 결과 진균 감염인 경우나 호중구 감소 기간이 긴 경우 사망률이 높았으며 그 중에서도 골수 기능이 회복 여부가 환자들의 생존과 통계적으로 유의한 연관이 있었다.(P=0.01). 결론 : 급성 백혈병 환자들에서의 감염은 관해유도화학요법 후 초래되는 호중구 감소와 유의한 관계가 있으며, 폐렴 및 패혈증의 임상양상을 보이는 경우가 흔하고, 원인균별로는 그람 음성균이 주류를 이루나 최근 그람 양성균 감염이 증가하는 추세를 보였다. 감염의 예후 인자로는 진균 감염인 경우나 호중구 감소 기간이 긴 경우 사망률이 높았으나 골수 기능의 회복여부가 결정적인 역할을 하는 것으로 나타나, 향후 급성 백혈병 환자의 치료에 있어 감염 빈도의 감소와 감염에서의 조속한 회복을 위해서는 골수 기능의 회복에 중점을 두어야 할 것으로 사료된다. Background : Infection is one of the most important and fatal complications in patients with acute leukemia. The characteristics of infection in acute leukemic patients are different from those in other diseases by the lack of normal inflammatory responses or distinct clinical evidence except fever. To improve the outcome of acute leukemia, it is very important to recognize the risk factors, patterns and prognostic factors of acute leukemia. Methods : We analyzed retrospectively the patterns of infection from 113 febrile patients with acute leukemia from January, 1988 to December, 1995. To determine the prognostic factors and the outcome of infection, the following variables were analyzed: the presence of neutropenia, use of chemotherapeutic agents, type and site of infections, isolated organisms, gastrointestinal decontamination, duration of neutropenia, and bone marrow recovery. Results : Out of 113 febrile patients with acute leukemia, 84 infection episodes(74%) occurred after chemotherapy. The mean duration of neutropenia was 18±13 days. The incidence of microbiologically-documented infection(MDI) was 35%(40/113). Pneumonia was the most common infection(26%), followed by primary sepsis(24%), catheter-related infection(9%). In cases of MDI, 63% were caused by gram-negative bacteria, followed by gram-positive bacteria(28%), and fungi(10%). Escherichia coli(25%) was the most common isolated in MDI. Regarding the prognostic factors in cases with infections, the recovery of bone marrow function was the only statistically significant factor(P=0.01). Conclusion : Infection has been a major cause of morbidity and mortality in acute in acute leukemic patients. To prevent infection and thereby improve the prognosis of acute leukemia, restoration of bone marrow function at early stage is important.

Vibrio parahemolyticus에 대한 한방처방 [方藥合編] 및 그 단미제의 항균활성에 관한 연구

마진열,김진숙,신순식,정규용,박갑주 한국한의학연구원 1999 한국한의학연구원논문집 Vol.5 No.1

Vibrio are become prevailing if superficial temperature of ocean is raised and their activities of area are expanded and most of ocean creatures (fishes, oysters etc) are polluted with vibrio. The one who has taken these polluted fishes and aysters uncooked caused foodpoisoning and diarrhea from Vibrio. Frequencise of these diseases breakout is disposed in westsea shore of Korea. According to ancient and traditional Korean medical book -『Bangyak Happyeon』(Collection of Local Medicines, 1884) - and their single prescibes, we carried out experiment check the activities of natural medicinal effects on Vibrio parahemolyticus. The prescriptions of trial materials are processed from extraction boiling water and 80% methanol and followed freeze dried and adsorbed to every discs in dosage of 10mg. Gentamycin of 10mg were used for control. The result of compound prescription displayed special diseases in antimicrobial activities of boiling water and MeOH extraction compared with control. In compound prescription, extraction MeOH of Sashinhwan(clear zone : 17mm) presented extraordinaire antimicrobial activity. In single prescription, extraction of boiling water (clear zone : 16mm) and MeOH(clear zone : 18mm) of Fructus Chebulae presented extraordinaire antimicrobial activity. The MBC of Fructus Chebulae extracts was expressed in boiling water (1.28mg/ml) and MeOH(0.64mg/ml).

Monoamine Oxidase 의 활성 변화에 의한 기미론 연구

黃今熙,마진열,金仁洛 대한본초학회 1999 大韓本草學會誌 Vol.14 No.1

To explain the theory of KIMI which is the theory of therapeutics in oriental medicine, the monoamine oxidase(MAO) activities were determined in the brain and liver of mouse which was orally adminstered cold and hot drugs, and forced swimming in cold and hot water. The MAO plays a central role in the metabolism of many amines including the neurotransmetter monoamines. MAO is a flavoprotein found exclusively in the mitochondrial outer membrane, occuring in the MAO-A and MAO-B subtypes. MAO-A deaminates serotonin and noradrenaline, whereas MAO-B prefers penylethylamine and benzylamine as substrates. Serotonin is important neurotransmetter for the control of body temperature. Coptis japonica Makino was selected as the cold drug, and Radix Aconiti lateralis preparata was as the hot drug. Coptis japonica Makino elevated the MAO-A activity which was increased by cold stress, whereas it inhibited the MAO-B activity which was increased by cold stress. Coptis japonica Makino elevated the MAO-A activity which was decreased by heat stress, whereas it inhibited the MAO-B activity which was increased by heat stress. Radix Aconiti lateralis preparata inhibited the MAO-A activity which was increased by cold stress, whereas it inhibited the MAO-B activity which was increased by heat stress.

백반증의 PUVA치료반응에 관한 연구(제1보)

박장규,성열훈,성범진,김영호,이증훈 충남대학교 의과대학 지역사회의학연구소 1990 충남의대잡지 Vol.17 No.2

The authors evaluated response to PUVA therapy in 44 vitiligo petients who did'nt show the spontaneous re-pigmentation. In the responded group to PUVA therapy after systemic administration of 8-methoxy psoralen, average MPD of female patients was 3.4 times higher than males, interestingly. Number of irradiation of UVA was similar between male and female, but total doses of female patients group were about 2 times than males till the first re-pigmented spots were noted. The response to PUVA therapy was quite different according to duration of disease and site of lesion. Therefore, we suppose that it is necessary to consider sex of patient, duration of disease, location of vitiligo before making a plan of PUVA therapy.

多樣한 黃芩藥鍼製劑의 安全性 및 效能에 關한 硏究

김호경,마진열,전원경,윤수영,강은정,주혜정,고병섭 한국한의학연구원 1997 한국한의학연구원논문집 Vol.3 No.1

In order to detect the safety and effect of various aqua-acupunctures from Scutellariae Radix, the modifications of boiling, filtration and dilution were employed for the manufacture of aqua-acupunctures. We injected 0.2cc of aqua-acupunctures into Joksamri(足三里) of rat, repeatedly. we compared subacute toxicity of them with saline group, distilled water(D.W.) group, acupuncture group and control group. The results were summarized as follows: 1. The groups were all healthy and alive, and there was no special abnormality in physical condition and autopsy. And there were not any toxic symptoms in repeating application of aqua-acupunctures to the rat, including changes of body weight, organ weight, haematological examination and serum biochemical test. 2. There was slight change of body weight in acupuncture group : We could see significance after 3 days (p<0.05) and after 7 days (p<0.001) in body weight loss. After 9 days, all tested groups were suppressed in body weight increment. 3. Result of organ weight: In Palking aqua-acupuncture(D-2 group), saline group and acupuncture group there were some statistical significance. Especially, acupuncture group revealed significant result in liver and spleen than aqua-acupunctures. From this result, we could suggest that the efficacy of acupuncture was preceded herbal medicine. 4. In serum biochemical test, we examined glucose(GLU), triglyceride(TG) and cholesterol(CHOL). In comparison with control group, the diluted 10 times of hwanggum aqua-acupuncture (× 10 group) was recognized significant decrease of glucose, but the diluted 100 times of Hwanggum aqua-acupuncture (× 100 group), D-2 group, saline group were confirmed significant increment. There was not any meaningful change of CHOL in all of tested group, excepting the acupuncture group was exhibited statistically significant decrease(p<0.05). In TG level all tested group except complex injection of standard compound (CPA group) and HG, there were significant value in statistically. The diluted solution was more significant decrease than Hwanggaum aqua-acupuncture(HG). The mutual relationship of components of aqua-acupuncture tended to decrease level of TG, regardless of its concentration. In acupuncture group, we gained some interesting result in meaningful decrease in TG. 5. Haematological examination showed significant increment of gramulocytes(GR) in all tested groups except Hwanggum aqua-acupuncture. And the diluted solutions of HG expressed very high increment of them(p<0.001). The GR and Mean Corpuscular Volume(MCV) of acupuncture group showed statistical significance.

접촉성 피부염 환자와 정상인의 첩포검사에 관한 연구

박장규,성열훈,성범진 충남대학교 의과대학 지역사회의학연구소 1989 충남의대잡지 Vol.16 No.2

Numerous studies on epidemiological aspects of contact dermatitis due to various causative antigens have been reported n recent year in Korea. However, degree of sensitization in healthy persons was not reported till now in Korea. We have performed the patch test on patients with contact dermatitis and healthy persons. The study results were as follows; 1. Percent of positive reaction in patients with contact dermatitis was 45.9%. The common allergens of positive reactions were nickel sulfate(21.6%), potassium dichromate (10.8%), neomycin sulfate (8.1%), Balsam of Peru (8.1%), mercuric chloride, ammoniated (5.4%), p-phenylenediamine(5.4%), cinnamic alcohol(5.4%), ethylenediamine dihydrochloride (2.7%), formaldehyde(2.7%), wool wax alcohol(2.7%), thimerosal (2.7%), mercaptobenzothiazole(2.7%), imidazolidinyl urea(2.7%) in order of frequency. 2. Percent of positive reaction in healthy persons was 10.3% and the common allergens were mercuric chloride, ammoniated(5.2%), nickel sulfate(3.1%), cinnamic alcohol(2.1%), ethylenediamine dihydrochloride(1.0%), formaldehyde(1.0%), epoxy resin(1.0%), in order of frequency. We conclude that allergens associated with metals and drugs seem to be the dominant causes of contact dermatitis and healthy persons.

GMAHN에서의 네트워크 성능 향상에 관한 연구

金在浩,李光培,金玄郁,劉忠烈,河載承,裵振勝 明知大學校 産業技術硏究所 2008 産業技術硏究所論文集 Vol.27 No.-

Recently, Wire-rireless combination study is being studied very actively with the advent of Ubiquitous environment, using wireless device such as PDA, Cellular Phone loading multi-hop communication that connects wired internet network very easily and controls the system and shares the information in anyplace. The past algorithm, wired and wireless combination that connect between Ad Hoc Network and wired network is being focused only on the study of heterogeneous network interface. The proposed algorithm established independent multi-path between source node and destination node to prevent data loss when errors happen in route. The proposed algorithm established independent multi-path between source node and destination node to prevent data loss, and trasmitted data packet to destination node very quickly and promoted network reliabiliy when errors happen in route.

煎湯에 의한 黃芩 成分 移行率에 관한 硏究I

고병섭,주혜정,마진열,박갑주,안상우 한국한의학연구원 1996 한국한의학연구원논문집 Vol.2 No.1

In order to improve the qualities of boiling extract the availability of standard compounds was investigated in extract of Scutellariae Radix. The standard compounds used baicalin(1), baicalein(2), and wogonin(3). The availabilities were analyzed by thin layer chromatography(TLC) and high performance liquid chromatography(HPLC) on C18 column. The extract of RS-4(W/V=10g/100ml) showed the highest availabilities as 7.95% of baicalin(1), 1.04% of baicalein(2), and 0.31% of wogonin(3).

피리딘 치환제와 2-Bromoacetophenone과의 반응에 대한 속도론적 연구

조영재,김용건,김장열,경진범 한양대학교 이학기술연구소 2000 이학기술연구지 Vol.2 No.

메탄을 용매내에서 2-bromoacetophenone과 피리딘 치환체(3-CH3, 3,5-(CH3)2, H, 3-Cl)와의 반응속도를 여러 온도와 농도 조건하에서 전도도법으로 측정하였다. 이 때 반응속도상수는 온도와 피리딘 농도가 증가함에 따라 증가하였으며, 피리딘 치환체에서 치환기의 전자주게 능력이 클수록 반응속도는 증가하였다. 이들 반응속도상수로부터 활성화 엔탈피, ?H≠, 활성화 엔트로피, ?S≠, Hammett 반응상수, p 및 Bsonsted 상관계수, B를 결정하였다. 이 때 ?H≠는 상대적으로 낮은 양의 값을 갖는 반면, ?S≠는 큰 음의 값을 얻었으며, 친핵체의 치환기 효과인 p와 Bronsted 상관계수인 B는 40℃에서 각각 -2.49 및 0.38의 값을 나타내었다. 이러한 결과로부터 본 반응은 dissociative 이분자성 반응으로 진행됨을 알 수 있었다. Kinetics of 2-bromoacetophenone with substituted pyridines (3-CH3, 3,5-(CH3)2, H, 3-Cl) in methanol have been also studied using conductometric method at various temperatures and concentrations. Rate constants are increased with the increment of both temperature and concentration. They are also increased with more electron-donating ability of substituent in nucleophile. The activation enthalpy, ?H≠, the activation entropy, ?S≠, Hammett reaction constant, p and Bronsted coefficient, B are evaluated from the rate constants. The ?H≠ values are positive relatively small while the ??S≠?values are relatively large, and the corresponding coefficients, p and B are -2.49 and 0.38 at 40℃, respectively. From these results, it could be concluded that this reaction proceeds with the dissociative bimolecular pathway.

Oral Administration of Novel Oriental Medicine, KIOM-C, Protect against Influenza Virus

Jin Yeul Ma, Eun Ha Kim, Jun Han Lee, Min-Suk Song, Yun Hee Baek, Young Ki Choi 충북대학교 동물의학연구소 2012 Journal of Biomedical and Translational Research Vol.13 No.2

The influenza virus is an important respiratory risk affecting humans, and effective treatments are needed. Some oriental medicines are currently applied for treatment of common colds as well as influenza infection. Previous studies have reported that the therapeutic properties of MA-128 are effective for treatment of psoriasis antiasthmatic and atopic dermatitis. In this study, we investigated the therapeutic properties of the novel herbal medicine, MA-128, for treatment of influenza virus infection by oral administration. MA-128 is an active natural biological compound from herbal-marine origin. The results showed that oral administration of MA-128 in mice could confer a survival benefit against Type A influenza virus infection. Daily oral administration of MA- 128 resulted in delayed death in infected mice for three days against mouse adapted H3N2 (A/Philippines/2/82). However, it protected more than 60% of mice from lethal infection of 2009 pandemic H1N1 (A/Korea/CJ01/2009) influenza virus. In addition, lung viral titers were significantly reduced at seven days post infection (~100 times) compared with mock-treated mice and viruses were cleared at 9 dpi only in the MA-128 treated groups. This study demonstrated the potential of the novel herbal medicine, MA-128, as an herbal remedy against influenza A viruses.