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Jinlong Zhang,Jingkun Lu,Bing Liu,Qiuyue Liu,Fan Jin,Miaojun Zhang,Yerong Liu,Yujun Song,Chenhui Dong,Wanyi Zhang,Ningxu Han,Xu Deng,Feng Xing 한양대학교 세라믹연구소 2019 Journal of Ceramic Processing Research Vol.20 No.S1
Quantification of viable spores is a time taking task due to the lack of rapid, efficient and accurate methods. This studypresented a simple spectrophotometric method for the detection of viable spores based on spore’s property of losing refractivityduring the germination process. By comparison of the results obtained by both spectrophotometric method and colonycounting method, a good linear correlation (R2 = 0.99) was achieved between viable spore concentration and OD loss underappropriate conditions. To avoid interference from ungerminable spores and vegetative cells, a turbidity complementationstrategy of keeping the initial concentration of spore suspensions at the same and relatively lower level was required. Thecalibration equation developed could be used to predict the viable spore yield produced in a series of fermentation experiments. The experimental results proved that this novel spectrophotometric method was sensitive, rapid, and easy to performcompared to conventional colony counting method.
Jun-Ying Zhang,Hyeon-Jin Sun,In-Ja Song,Hong-Gyu Kang,Suk-Min Ko,Yong-Ik Kwon,Il-Woung Kim,Jae-Hoon Kim,Hyo-Yeon Lee 한국육종학회 2014 한국육종학회 심포지엄 Vol.2014 No.07
With the purpose of improving ginsenoside production in Korean wild ginseng (Panax ginseng Meyer) mutant adventitious root lines, a synthetic gene encoding squalene synthase (PgSS2) was placed under the control of 35S promoter and transferred to Panax ginseng. Embryogenic callus obtained from ginseng adventitious root lines were transformed by infection with A. tumefaciens strain EHA105 containing the PgSS2 gene. Ten phosphinothricin-resistant plants were generated on selection medium, and the transgene integration and expression in these plants were confirmed by PAT test strip, RT-PCR and Southern hybridization. Ginsenoside analysis by HPLC revealed that the total contents of the 8 ginsenoside types (Rg1, Re, Rf, Rh1, Rb1, Rc, Rb2, Rd) in transgenic adventitious root lines were about 1.6-fold higher than that of the mutant control line (MCL1). This transformation method may facilitate the improvement of Panax ginseng in terms of the accumulation levels of ginsenoside.
Shuang Wang,Qian Yang,Zhi-Hua Liu,Lei Sun,Dan Wei,Jun-Zheng Zhang,Jin-Zhu Song,Yun Wang,Jia Song,Jin-Xia Fan,Xian-Xin Meng,Wei Zhang 한국미생물학회 2011 The journal of microbiology Vol.49 No.1
A moderately halophilic bacterial strain 15-13^T, which was isolated from soda meadow saline soil in Daqing City, Heilongjiang Province, China, was subjected to a polyphasic taxonomic study. The cells of strain 15-13^T were found to be Gram-negative, rod-shaped, and motile. The required growth conditions for strain 15-13^T were: 1-23% NaCl (optimum, 7%), 10-50°C (optimum, 35°C), and pH 7.0-11.0 (optimum, pH 9.5). The predominant cellular fatty acids were C18:1 ω7c (60.48%) and C16:0 (13.96%). The DNA G+C content was 67.6 mol%. Phylogenetic analysis based on 16S rRNA gene sequence comparisons indicated that strain 15-13^T clustered within a branch comprising species of the genus Halomonas. The closest phylogenetic neighbor of strain 15-13^T was Halomonas pantelleriensis DSM 9661^T (98.9% 16S rRNA gene sequence similarity). The level of DNA-DNA relatedness between the novel isolated strain and H. pantelleriensis DSM 9661^T was 33.8%. On the basis of the phenotypic and phylogenetic data, strain 15-13^T represents a novel species of the genus Halomonas, for which the name Halomonas alkalitolerans sp. nov. is proposed. The type strain for this novel species is 15-13^T (=CGMCC 1.9129^T =NBRC 106539^T).
Studies on the Small Body Size Mouse Developed by Mutagen N-Ethyl-N-nitrosourea
Zhang, Qian-Kun,Cho, Kyu-Hyuk,Cho, Jae-Woo,Cha, Dal-Sun,Park, Han-Jin,Yoon, Seok-Joo,Zhang, ShouFa,Song, Chang-Woo Korean Society of ToxicologyKorea Environmental Mu 2008 Toxicological Research Vol. No.
Mutant mouse which show dwarfism has been developed by N-ethyl-N-nitrosourea (ENU) mutagenesis using BALB/c mice. The mutant mouse was inherited as autosomal recessive trait and named Small Body Size (SBS) mouse. The phenotype of SBS mouse was not apparent at birth, but it was possible to distinguish mutant phenotype from normal mice 1 week after birth. In this study, we examined body weight changes and bone mineral density (BMD), and we also carried out genetic linkage analysis to map the causative gene(s) of SBS mouse. Body weight changes were observed from birth to 14 weeks of age in both affected (n = 30) and normal mice (n = 24). BMD was examined in each five SBS and normal mice between 3 and 6 weeks of age, respectively. For the linkage analysis, we produced backcross progeny [(SBS${\times}$C57BL/6J) $F_1{\times}$ SBS] $N_2$ mice (n = 142), and seventy-four microsatellite markers were used for primary linkage analysis. Body weight of affected mice was consistently lower than that of the normal mice, and was 43.7% less than that of normal mice at 3 weeks of age (P < 0.001). As compared with normal mice at 3 and 6 weeks of age, BMD of the SBS mice was significantly low. The results showed 15.5% and 14.1 % lower in total body BMD, 15.3% and 8.7% lower in forearm BMD, and 29.7% and 20.1% lower in femur BMD, respectively. The causative gene was mapped on chromosome 10. The map order and the distance between markers were D10Mit248 - 2.1 cM - D10Mit51 - 4.2 cM - sbs - 0.7 cM - D10Mit283 - 1.4cM - D10Mit106 - 11.2cM - D10Mit170.
Zhang, Kan,Kim, Hwan-Jin,Lee, Jeong-Taik,Chang, Gee-Woo,Shi, Xinjian,Kim, Wanjung,Ma, Ming,Kong, Ki-jeong,Choi, Jae-Man,Song, Min-Sang,Park, Jong Hyeok Wiley-VCH 2014 CHEM SUS CHEM Vol.7 No.9
<P>A 2H-MoS2 (H=hexagonal) ultrathin nanomesh with high defect generation and large porosity is demonstrated to improving electrochemical performance, including in lithium-ion batteries (LIBs) and the hydrogen evolution reaction (HER), with the aid of a 3D reduced graphene oxide (RGO) scaffold as fast electron and ion channels. The 3D defect-rich MoS2 nanomesh/RGO foam (Dr-MoS2 Nm/RGO) can be easily obtained through a one-pot cobalt acetate/graphene oxide (GO) co-assisted hydrothermal reaction, in which GO, cobalt and acetate ions are co-morphology-controlling agents and defect inducers. As an anode material for LIBs, Dr-MoS2 Nm/RGO has only a 9% capacity decay at a 10?C discharge rate versus 0.2?C with stable cyclability at the optimized composition (5?wt% RGO to MoS2 and 2?mol% Co to Mo), and significantly achieves 810?mA?h?g(-1) at a high current density of 9.46?A?g(-1) over at least 150?cycles. Moreover, Dr-MoS2 Nm/RGO exhibits superior activity for the HER with an overpotential as low as 80?mV and a Tafel slope of about 36?mV per decade. In contrast to the MoS2 nanosheet/RGO (MoS2 Ns/RGO), which is synthesized in the absence of cobalt ions, Dr-MoS2 Nm/RGO provides high interconnectivity for efficient lithium-ion transport, and rich defects as electrochemically active sites. DFT is used to prove the existence of rich defects due to anion replacement to become a Co-Mo-S atomic structure, releasing inert basal planes to active sites.</P>
Bioinspired Hierarchical Tin Oxide Scaffolds for Enhanced Gas Sensing Properties
Song, Fang,Su, Huilan,Han, Jie,Lau, Woon Ming,Moon, Won-Jin,Zhang, Di American Chemical Society 2012 The Journal of Physical Chemistry Part C Vol.116 No.18
<P>Nature is greatly capable of providing inspiration for the novel design of functional materials. Herein, the efficient bioreactors’ construction of pollen grains inspires us to mimic them for superior gas sensing application. By developing a facile two-step soakage process and subsequent calcinations, the bioreactors are mimicked fully: (I) biosensitive pollen coats on pollen grains are replaced by gas sensitive tin oxide (SnO<SUB>2</SUB>) coats, and (II) the fine hierarchical scaffolds are maintained by the self-support of newly formed SnO<SUB>2</SUB> coats. For gas sensing application, as-fabricated SnO<SUB>2</SUB> microreactors exhibit high and fast responses to nitrogen dioxide (219.5 to NO<SUB>2</SUB> of 50 ppm) and other gases. The good sensing properties should be indeed ascribed to the specific construction of microreactors, which shows elaborate hierarchical porous structures and large accessible space/surface area favorable for both gas molecule transports and sensing reactions. This present strategy provides us with new insight on the exploring of effective and low-cost gas sensors, and it could further extend to other pollen grains of numerous different morphologies and other types of bioreactors that are abundant in nature.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jpccck/2012/jpccck.2012.116.issue-18/jp2118136/production/images/medium/jp-2011-118136_0010.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/jp2118136'>ACS Electronic Supporting Info</A></P>
Song, Xu-Ping,Tian, Jin-Hui,Cui, Qi,Zhang, Ting-Ting,Yang, Ke-Hu,Ding, Guo-Wu Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.19
This meta-analysis was performed to assess the implementation effects of clinical pathways in patients with gastrointestinal cancer. A comprehensive search was conducted in the Cochrane Library, PubMed, EMBASE, Web of Science and Chinese Biomedical Literature Database (from inception to May 2014). Selection of studies, assessing risk of bias and extracting data were performed by two reviewers independently. Outcomes were analyzed by fixed-effects and random-effects model meta-analysis and reported as mean difference (MD), standardized mean difference (SMD) and odds ratio (OR) with 95% confidence intervals (CI). The Jadad methodological approach was used to assess the quality of included studies and the meta-analysis was conducted with RevMan 5.1 software. Nine citations (eight trials) involving 642 patients were included. The aggregate results showed that a shorter average length of stay [MD = -4.0; 95% CI (-5.1, -2.8); P < 0.00001] was observed with the clinical pathways as compared with the usual care. A reduction in inpatient expenditure [SMD = -1.5; 95% CI (-2.3, -0.7); P = 0.0001] was also associated with clinical pathways, along with higher patient satisfaction [OR = 4.9; 95% CI (2.2, 10.6); P < 0.0001]. Clinical pathways could improve the quality of care in patients with gastrointestinal cancer, as evidenced by a significant reduction in average length of stay, a decrease in inpatient expenditure and an improvement in patient satisfaction. Therefore, indicators and mechanisms within clinical pathways should be a focus in the future.
Zhang, Kan,Kim, Hwan-Jin,Shi, Xinjian,Lee, Jeong-Taik,Choi, Jae-Man,Song, Min-Sang,Park, Jong Hyeok American Chemical Society 2013 Inorganic chemistry Vol.52 No.17
<P>Morphology-controlled MoS<SUB>2</SUB> nanosheets were successfully synthesized with the aid of graphene/acid coexistence by a one-pot hydrothermal method. The ultrathin MoS<SUB>2</SUB> nanosheets were self-assembled into a cockscomb-like structure with an exposed (100) facet on graphene sheets, which is in strong contrast to large aggregate MoS<SUB>2</SUB> plates grown freely on graphene sheets without acetic acid. The ultrathin MoS<SUB>2</SUB> nanosheets displayed excellent rate performance for Li storage (709 mAh·g<SUP>–1</SUP> capacity at 8320 mA·g<SUP>–1</SUP> discharging rate) and superior charge/discharge cyclability.</P><P>Ultrathin MoS<SUB>2</SUB> nanosheets displayed excellent rate performance for Li storage (709 mAh·g<SUP>−1</SUP> capacity at 8320 mA·g<SUP>−1</SUP> discharging rate) and superior charge/discharge cyclability.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/inocaj/2013/inocaj.2013.52.issue-17/ic400735f/production/images/medium/ic-2013-00735f_0005.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/ic400735f'>ACS Electronic Supporting Info</A></P>