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Prediction of Bull Fertility by Capacitation Status
Yoo-Jin Park,Woo-Sung Kwon,Sung-Jae Yoon,Kyu-Hyun Jeong,Kamla Kant Shukla,El-sayed A. Mohamed,Myung-Geol Pang 한국동물생명공학회(구 한국동물번식학회) 2011 발생공학 국제심포지엄 및 학술대회 Vol.2011 No.1
The prediction of male fertility is of paramount importance for breeding animal herds when artificial insemination is applied. While the male fertility assays provide valuable quantitative data, they yield limited information concerning the functional competence of the spermatozoa. The objective of this study was to standardize a method for predicting in vivo fertility in bulls using the capacitation status that was assessed by chlortetracycline (CTC) staining. To optimize the capacitation process, sperm were treated with various concentrations of heparin (0, 10, 20, 50, and 100 μg/mL) and incubated for 10, 20, and 30 min each at 39℃ in 5% CO2. We found that maximum capacitation condition obtained from 10 μg /mL heparin treated sperm cells for 20 min (p<0.05). Optimized methods were used to determine the fertility of 17 batches of frozen bull semen representing a wide range of field fertility levels as indicated by non-return rates (NRR) (35.29% 93.18%). There was no significant correlation between NRR and the percentage of capacitated spermatozoa (B type) and non-capacitated spermatozoa (F type). However, acrosome reacted spermatozoa (AR type) was significantly correlated with NRR (p<0.01). To determine the normal range for the AR type, lower limits of the AR (%) were established as 23% for low fertility (NRR < 75%) using receiver operating characteristic curve. The overall accuracy of the assay was 88.24% for low fertility, sensitivity and specificity were 81.82 and 100%, respectively. These results indicate that capacitation status as measure by CTC staining is a useful predictor of male fertility. Therefore, low and high fertility bulls can be identified primarily by the functional capacitation status.
예비교사의 개인 혁신성, 자기효능감, 교수효능감이 교육 현장의 기술수용에 미치는 영향 - Covid-19 유행 전후의 차이를 중심으로 -
진현정(Hyun-Jeong Jin),방희석(Hee-Suk Pang) 한국산학기술학회 2023 한국산학기술학회논문지 Vol.24 No.2
본 연구는 초등 예비교사의 Covid-19 유행 이전과 이후의 개인 혁신성, 자기효능감, 교수효능감(교과지도 효능감, 정보화기자재 활용 효능감)이 기술수용 의도에 미치는 영향을 파악하고자 하였다. 초등 교원양성대학에 재학 중인 3학년 학생을 대상으로 2018년과 2022년, 4년 간격으로 2차례의 설문이 진행되었고, 358부의 응답을 최종 분석에 사용하였다. 코로나 이전과 이후의 예비교사의 개인 혁신성, 자기효능감, 교수효능감(교과지도 효능감, 정보화 기자재 활용 효능감), 기술수용 의도의 차이를 분석한 결과 Covid-19 유행 후에 정보화 기자재 활용 효능감과 기술수용 의도가 높은 것으로 나타났다. 개인 혁신성, 자기효능감, 정보화기자재 활용 효능감, 교과지도 효능감이 예비교사의 기술수용 의도에 미치는 영향을 알아보기 위하여 다중회귀 분석을 실시한 결과 정보화기자재 활용 효능감, 자기효능감, 개인 혁신성은 기술수용 의도에 정적인 영향을 미쳤고, 교과지도 효능감은 부적인 영향을 미치는 것으로 확인되었다. 본 연구의 결과는 정보화기자재 활용 효능감, 자기효능감이 교육 분야의 기술 혁신 수용에서 두드러진 예측 요인이며, 교과 지도에 자신있는 예비교사는 정보화기자재 도입에 소극적임을 확인할 수 있었다. This study aimed to determine the effects of personal innovativeness, self-efficacy, and teaching efficacy of pre-service elementary teachers on technology adoption before and after the Covid-19 pandemic. Two surveys were conducted at a four-year interval, in 2018 and 2022, targeting third-year students attending elementary teacher training colleges. A number of 358 responses were included in the analysis. We established that pre-service teachers had higher post-Covid-19 pandemic innovativeness, self-efficacy, teaching efficacy (subject teaching efficacy, efficacy in using informatization equipment), intention to accept technology, efficacy of using informatization equipment, and intention to adopt technology. Multiple regression analysis was conducted to examine the effects of innovativeness, self-efficacy, efficacy in using informatization equipment, and subject teaching efficacy on pre-service teachers technology adoption. It was confirmed that the subject teaching efficacy had a negative effect. The results of this study confirm that self-efficacy and the efficacy of using informatization equipment are prominent predictive factors in the adoption of technological innovation in the field of education, and pre-service teachers who are confident in subject guidance are passive in accepting informatization equipment.
모체 혈액을 이용한 태아 유핵 적혈구 분리기법에 따른 산전 유전진단
전여진 ( Yeo Jin Jeon ),권경훈 ( Kyung Hun Kwon ),황효성 ( Hyo Sung Hwang ),이소현 ( So Hyun Lee ),방명걸 ( Myung Geol Pang ),안정자 ( Jung Ja Ann ),전선희 ( Sun Hee Chun ),김영주 ( Young Ju Kim ) 대한산부인과학회 2007 Obstetrics & Gynecology Science Vol.50 No.6
목적: 태아 유핵 적혈구의 분리를 하여 FISH에 의해 산전 태아의 성과 염색체 이수성을 확인하기 위해 본 연구를 시행하였다. 연구방법: 말초혈액샘플은 임신 11주에서 24주 사이의 37명의 여성으로부터 수집되었다. 모체혈액으로부터 double density gradient와 magnetic activating cell sorting방법을 시행한 후 Kleihaur-Betke 염색을 통해 태아 유핵 적혈구를 형태학적으로 관찰하였다. K-B positive한 유핵 적혈구에 CEP X와 CEP Y probe를 이용한 FISH를 시행하여 유핵 적혈구의 성별 확인을 통해서 태아 기원 유무를 확인하였다. 결과: 10 ml의 혈액에서 분리한 K-B positive한 유핵 적혈구는 평균 17.3 (±17.2)개였으며, 최대 54개의 유핵 적혈구가 관찰되었다. 이 중 양수검사를 확인한 총 18명의 산모에서 분리한 유핵 적혈구의 67.3%에서 CEP Y probe의 signal을 확인하였다. 결론: 우리는 분리된 태아 유핵 적혈구를 이용해서 FISH 기법을 통해 태아 성별을 판정할 수 있을 뿐만 아니라 태아의 염색체의 이수성을 진단하는데 있어서의 활용 가능성을 확인할 수 있었다. 분리한 유핵 적혈구 중 모체 기원의 유핵적혈구가 포함되어 있어서 추후 이를 선별해 내는 방법에 대한 연구가 필요할 것으로 사료된다. Objective: To identify prenatal fetal sex and chromosomal aneuploidies by FISH using isolation of fetal nucleated RBCs. Methods: peripheral blood samples was collected from 37 women between 11 and 24 weeks of gestation. we tried to enrich nucleated RBCs morphologically by Kleihaur-Betke staining after double gradient centrifugation and magnetic activating cell sorting (MACS) from maternal blood. Fluorescence in situ hybridization (FISH) analyses with CEP X and CEP Y probes for K-B positive nucleated RBCs were performed to detect whether fetal cells were existed among nucleated RBCs by observation of sex chromosomes. Results: The average number of K-B positive nucleated RBCs separated from 10ml of maternal blood was 17.3 (±17.2) and the maximum number of nucleated RBCs was 54. We observed FISH signals in nucleated RBCs separated from 18 pregnant women, and Y probe signals were observed in 67.3% of nucleated RBCs separated from 10 pregnant women. Conclusion: We confirmed that separated nucleated fetal RBCs can be used to identify fetal sex and chromosomal aneuploidies by FISH. Since nucleated RBCs from maternal origin were not excluded, further studies are needed to overcome this limitation.
방만식(Man-Sik Pang),최재형(Jae-Hyeong Choi),김우진(Woo-Jin Kim),김상현(Sang-Hyun Kim) 한국조명·전기설비학회 2011 한국조명·전기설비학회 학술대회논문집 Vol.2011 No.5월
Recently, UV camera have attracted a great deal of interest from the view point of easy judgement. UV camera is used corona discharge. One of the most important and difficult problems to be solved filer design, materials and corona discharge. This paper is studied on the UV generation and shape analysis and corona pulse count according to the electrode distance and applied voltage. Also, Corona discharge characteristics in air are analyzed using prototype UV camera of Korea. UV generation due to surface discharge of AC is higher than that of DC.
방만식(Man-Sik Pang),최재형(Jae-Hyeong Choi),김우진(Woo-Jin Kim),김영석(Young-Seok Kim),김상현(Sang-Hyun Kim) 한국조명·전기설비학회 2011 조명·전기설비학회논문지 Vol.25 No.5
Recently, UV(ultra-violet rays) camera has attracted a great deal of interest from the view point of easy judgement in inspections and diagnoses of the safety of power equipment. Especilly, UV camera is applied the inspection of UV with corona discharge. One of the most important and difficult problems is the basic research of filter design, materials and corona discharge. In this paper, we will report on the UV generation, corona pulse count and the polarity effect of positive and negative DC surface discharge in air. Also, corona discharge characteristics are analyzed using prototype UV camera of Korea. UV generation and corona pulse count due to surface discharge in positive needle is higher than that of negative needle.
오염물질에 따른 변압기부싱 모델의 기중 코로나 방전 특성
방만식(Man-Sik Pang),김우진(Woo-Jin Kim),김영석(Young-Seok Kim),김상현(Sang-Hyun Kim) 한국조명·전기설비학회 2012 조명·전기설비학회논문지 Vol.26 No.5
The surface of bushing is contaminated with rain, dust, salt and others. A bushing with contaminations in air is serious problem in insulation. Therefore, it is important to understand the inspection and diagnoses of the safety. The ultra-violet rays(UV) camera has attracted interest from the view point of easy judgement. In this paper, we will report on the corona discharge characteristics of surface flashover model with contaminations in air. Also, UV images of discharge and corona pulse count in air are analyzed using prototype UV camera of Korea and a UV sensor with an optic lens. These results are studied at both AC and DC voltage under a non-uniform field.
Sung-Jae Yoon,Yoo-Jin Park,Woo-Sung Kwon,Kyu-Hyun Jeong,Sae-Mi Yoo,El-Sayed A. Mohamed,Myung-Geol Pang 한국동물생명공학회(구 한국동물번식학회) 2011 발생공학 국제심포지엄 및 학술대회 Vol.2011 No.1
The objective of this study was to examine the effect of various discontinuous Percoll washing conditions on sperm capacitation status and sperm survival. Frozen epididymal sperm samples from 3 bulls (0.5 ml plastic straws, 6% glycerol in egg yolk- Tris-glycerol extender) were thawed in 37℃ water bath for 1 min. To rule out individual variation, 3 sperm samples were mixed after thawing. The mixed samples then were randomly allocated to 12 treatment groups. Briefly, the spermatozoa were centrifuged for three different time lengths (10, 20, and 30 min) at two gravities (300 X g and 700 X g) through two concentrations of discontinuous Percoll density gradient of 1 ml 90%: 1 ml 45% Percoll and 2 ml 90%: 2 ml 45% Percoll to remove extender, debris, and dead spermatozoa. Sperm capacitation status and sperm survival were evaluated using combined Hoechst 33258 and chlortertracycline fluorescence staining assay. The acrosome reacted spermatozoa (AR pattern), uncapaciated spermatozoa (F pattern) and sperm survival were significantly correlated with centrifugation time (p< 0.01). Significantly decreased F pattern observed as centrifugal time increased. As centrifugal time increased, spermatozoa with F pattern decreased and spermatozoa showing AR pattern increased. Moreover, the dead spermatozoa were significantly stimulated in time-dependent manner. However, there were no significant differences in various force of centrifugation and Percoll volume. These results suggest that only centrifugation time significantly affects sperm capacitation status and sperm survival.
전력설비의 열화진단을 위한 코로나 방전의 자외선 이미지와 전기적 특성
최재형(Choi, Jae-Hyeeong),방만식(Pang, Man-Sik),김우진(Kim, Woo-Jin),김영석(Kim, Young-Seok),김상현(Kim, Sang-Hyun) 한국화재소방학회 2011 한국화재소방학회 학술대회 논문집 Vol.2011 No.추계
최근, 전력설비의 열화진단 및 안전검사에 있어 편의성과 활용성의 측면에서 자외선 카메라가 많은 관심을 받고 있다. 자외선 카메라는 전력설비의 코로나 방전에 의한 자외선 검출을 응용한 기기로서 열화지점과 열화정도를 이미지 형태로 직관적으로 나타낼 수 있다. 따라서 전력설비의 정확한 열화진단을 위해서는 코로나 방전에 대한 전기적 특성과 자외선 특성에 대한 이해가 중요하다. 본 논문에서는 전력설비의 열화진단을 위한 코로나 방전을 모의하고, 그에 따른 자외선 이미지와 전기적 특성에 관해 연구하였다.