운동 강도의 차이가 흰쥐의 혈액 성분과 적혈구의 형태에 미치는 영향

김부환,김종오,지용석,최대원,윤진환,정일규,김영욱,오봉석 한국스포츠리서치 2004 한국 스포츠 리서치 Vol.15 No.4

This study was to investigate the effects on hematological indices and heinz body formation in various intensity, Male Sprague-Dawley rats were randomly divided into the control group, the low-intensity exercise group, nigh-intensity exercise group (n=7 in each group). Animals of the low-intensity exercise groups were put on exercise 30 min per 1 day, 5 days per week for 6-weeks. Animals of the high-intensity exercise groups were put on exercise 120 min per 1 day, 5 days per week for 6-weeks. Red blood cell count (RBC), hemoglobin (Hgb), hematocrit (Hct), mean ceil volume (MCV), mean hemoglobin corpuscular (MCH), mean hemoglobin corpuscular concentration (MCHC) were analyzed in automated hematology analyzer (ABX Hematology MICROS 60) from 10㎕ of whole blood. Erythrocyte morphology were evaluated in super vital (Brilliant cresyl blue stain) stained blood films. In the present results, Hg, MCHC in the high-intensity exercise group was significantly decreased compared with the different exercise intensity and the control group. MCV in the high-intensity exercise group was significantly increased compared with the different exercise intensity end the control group. Heinz body formation in the high-intensity exercise group was significantly increased compared with the different exercise intensity and the control group. In the present study, it can be suggested that intensity of exercise can influence hematological variables and heinz body formation. These findings suggest that high intensity exercise induces oxidative damage to erythrocyte.

Anti-Inflammatory Effects of Ethyl Acetate Fraction from Cnidium officinale Makino on LPS-Stimulated RAW 264.7 and THP-1 Cells

Jeong, Jin-Boo,Hong, Se-Chul,Jeong, Hyung-Jin,Koo, Jin-Suk The Plant Resources Society of Korea 2012 한국자원식물학회지 Vol.25 No.3

This work aimed to elucidate the anti-inflammatory effects of ethyl acetate fraction from Cnidium officinale Makino with a cellular system of LPS-stimulated RAW 264.7 and THP-1 cells. Some key pro-inflammatory cytokines and mediators including NO, iNOS, $PGE_2$, COX-2, TNF-${\alpha}$, NF-${\kappa}B$ p50 and NF-${\kappa}B$ p65 were studied by sandwich ELISA and western blot analysis. Ethyl acetate fraction could significantly inhibit the production of NO, $PGE_2$, TNF-${\alpha}$, iNOS and COX-2 in LPS-stimulated cell than that of single LPS-stimulated. And ethyl acetate fraction suppresses the activation of NF-${\kappa}B$ p50 and NF-${\kappa}B$ p65. All the results showed that ethyl acetate fraction had a good anti-inflammatory effect on LPS-stimulated RAW264.7 and THP-1 cells. Taken together, the anti-inflammatory actions of ethyl acetate fraction from Cnidium officinale Makino might be due to the down-regulation of NO, $PGE_2$, TNF-${\alpha}$, iNOS and COX-2 via the suppression of NF-${\kappa}B$ activation.

Lunasin is prevalent in barley and is bioavailable and bioactive in in vivo and in vitro studies.

Jeong, Hyung Jin,Jeong, Jin Boo,Hsieh, Chia Chien,Hernandez-Ledesma, Blanca,de Lumen, Ben O Lawrence Erlbaum Associates, Publishers [etc.] 2010 Nutrition and cancer Vol.62 No.8

<P>Lunasin, a unique 43-amino acid peptide found in a number of seeds, has been shown to be chemopreventive in mammalian cells and in a skin cancer mouse model. To elucidate the role of cereals in cancer prevention, we report here the prevalence, bioavailability, and bioactivity of lunasin from barley. Lunasin is present in all cultivars of barley analyzed. The liver and kidney of rats fed with lunasin-enriched barley (LEB) show the presence of lunasin in Western blot. Lunasin extracted from the kidney and liver inhibits the activities of HATs (histone acetyl transferases), yGCN5 by 20% and 18% at 100 nM, and PCAF activity by 25% and 24% at 100 nM, confirming that the peptide is intact and bioactive. Purified barley lunasin localizes in the nuclei of NIH 3T3 cells. Barley lunasin added to NIH 3T3 cells in the presence of the chemical carcinogen MCA activates the expression of tumor suppressors p21 and p15 by 45% and 47%, decreases cyclin D1 by 98%, and inhibits Rb hyperphosphorylation by 45% compared with the MCA treatment alone. We conclude that lunasin is prevalent in barley, bioavailable, and bioactive and that consumption of barley could play an important role of cancer prevention in barley-consuming populations.</P>

Immune-Enhancing Activity of Hydrangea macrophylla subsp. serrata Leaves through Macrophage Activation

Jin Boo Jeong(정진부) 한국자원식물학회 2020 한국자원식물학회 학술심포지엄 Vol.2020 No.8

Schisandra Chinensis Inhibits Oxidative DNA Damage and Lipid Peroxidation Via Antioxidant Activity

Jin Boo Jeong,Hyung Jin Jeong 한국자원식물학회 2009 한국자원식물학회지 Vol.22 No.3

Schisandra chinensis have been traditionally used in Asia for the treatment of dyspnea, cough, mouth dryness, spontaneous diaphoresis, nocturnal diaphoresis, nocturnal emission, dysentery, insomnia and amnesia. The purpose of this study is to evaluate the protective effects of Schisandra chinensis on oxidative DNA damage and lipid peroxidation induced by ROS in non cellular and cellular system. DPPH radical, hydroxyl radical and hydrogen peroxide scavenging assay were used to measure the antioxidant activities. Phi X-174RF I plasmid DNA cleavage assay and intracellular DNA migration assay were used to evaluate the protective effect on oxidative DNA damage. MTT assay and lipid peroxidation assay were used for evaluating the protective effect on oxidative cell damage. It was found to scavenge DPPH radical, hydrogen peroxide and hydroxyl radical and it inhibited oxidative DNA damage, lipid peroxidation and cell death induced by hydroxyl radical. These data indicate that Schisandra chinensis possesses a spectrum of antioxidant and DNA-protective properties

대두 추출물 LES가 DNCB로 유도된 랫드의 알레르기성 접촉피부염에 미치는 영향

Jeong Sook Kim(김정숙),Young Mi Park(박영미),Jin Boo Jeong(정진부),Jae Hwan Lim(임재환),Hyung Jin Jeong(정형진),Eul Won Seo(서을원) 한국생명과학회 2011 생명과학회지 Vol.21 No.5

본 연구에서는 대두 추출물인 LES가 DNCB에 의해 인위적으로 접촉피부염을 유발시킨 랫드의 피부 회복능에 미치는 효과를 조사하였다. 알레르기 접촉피부염이 유발된 피부에 LES 17.6 ㎎/㎖를 3-5회 도포하여 피부 표면을 조직학적 분석하여 본 결과 각질이 제거된 깨끗하고 매끄러운 상태로 되었으며, 비후화된 표피층의 두께도 점차 정상군과 같은 두께로 회복시키는 효과를 나타냈다. 또한 LES는 체내 지질함량을 낮춰 정상수준으로 회복시키는 지질함량의 개선에 효과적으로 작용하고 있으며, 혈장 내 IgE의 수준도 정상군 수준으로 감소시키고 있으며, LES에 의해 염증 반응과 관련된 iNOS와 COX-2의 활성도 감소되어 피부의 과민반응 해소에 관여하는 것으로 생각된다. LES에 포함된 주 성분 중 하나인 lunasin은 세포 침투와 함께 핵 내로 이동하는 것으로 확인되어 전체 유전자 또는 일부 유전자의 발현을 조절하는 데 관여할 것으로 추측된다. 이러한 결과로 보아 LES는 알레르기성 접촉피부염에 매우 효과적인 치료 효과를 나타내고 있기 때문에 기존의 알레르기성 접촉피부염 치료제를 대체할 수 있는 제제로서 응용이 기대된다. In this study, we evaluated whether Lunasin Enriched Soybean (LES) suppressed 1-chloro-2,4-dinitrochlorobenzene (DNCB) induced allergic contact dermatitis in vivo. In the group treated with DNCB alone, erythema and hemorrhage in the skin were observed, while the application of LES after DNCB treatment modulated erythema and hemorrhage similar to the untreated group. In histopathological analysis of the skin, DNCB-treated groups showed increased skin thickness compared to the untreated groups, while the treatment of LES dose-dependently modulated skin thickness. Also, hypertrophy and hyperkeratosis of the epidermis, intracellular edema and infiltration of inflammatory cells were observed in the group treated with DNCB alone, while treatment of LES inhibited these inflammatory changes. DNCB also induced a significant up-regulation of iNOS and COX-2 expression, as compared to the untreated groups. However, the treatment of LES significantly decreased the down-regulation of iNOS and COX-2 expression, as compared to DNCB-treated groups. Also, LES reduced the IgE level in DNCB-stimulated groups. And lunasin from LES translocated the nucleus of the cells in the skin of Sprague-Dawley rats. Taken together, it is thought that LES has the potential to be used as an alternative treatment for allergic contact dermatitis.

Comparative Analysis among Four Citrus Species by DNA Microarray

Jeong Won Park,Seong Beom Jin,Kyung Hwan Boo,Sung Jin Chung,Su Hyun Yun,Adnan Al Bachchu,Jeong Hun Yun,Song I Han,Key Zung Riu,Jae Hoon Kim 한국육종학회 2012 한국육종학회지 Vol.44 No.3

Citrus is one of the major fruits produced in Korea. There are about 20 species mainly grown in Jeju Island, Korea. Four representative species, which are quite different in the shape of leaf and the taste of fruit, were selected and were used to profile the transcriptomes. These species are ‘Miyagawa Wase’ (C. unshiu Marcov.) satsuma mandarin, ‘Kiyomi’ (C. unshiu Marcov. x C. sinensis) mandarin hybrid, ‘Dangyuja’ (C. grandis) and ‘Natsudaidai’ (C. natsudaidai). Classification of the up-regulated and down-regulated genes using the Cluster of Orthologous Groups of proteins (COG) database reveals that the number of genes included in each group differed significantly among the four species. Several genes that showed significant differences in expression on the microarray were selected and their expression patterns were examined by reverse transcription- ploymerase chain reaction. Metabolic genes such as tyrosine decarboxylase and β-glucosidase ligase were found to be highly expressed in Miyagawa Wase, relative to other species. On the other hand, the expression level of mannose phosphate isomerase was lower in Miyagawa Wase. An efflux pump gene was found to be up-regulated in Kiyomi, whereas cinnamyl-alcohol dehydrogenase was down-regulated. β-carotene 15,15’-dioxygenase, which is involved in the vitamin metabolism, was up-regulated in Natsudaidai. Interspecific differentiations of gene expression are analyzed in terms of the metabolic pathways and their possible roles in citrus species.

Production of Recombinant Miraculin Protein Using Transgenic Citrus Cell Suspension Culture System

Jin, Seong Beom,Sun, Hyeon Jin,Bachchu, Md Adnan Al,Chung, Sung Jin,Lee, Jongwoo,Han, Song-I,Yun, Jeong Hun,Boo, Kyung Whan,Lee, Dongsun,Riu, Key Zung,Kim, Jae-Hoon The Korean Society for Applied Biological Chemistr 2013 Applied Biological Chemistry (Appl Biol Chem) Vol.56 No.3

Miraculin gene containing the N-terminal signal peptide was introduced into navel orange (Citrus sinensis Osb. var. brasiliensis Tanaka) callus cells by Agrobacterum-mediated transformation. Transgenic somatic embryos were screened on the shoot induction medium containing 25 mg hygromycin $L^{-1}$. Citrus callus cells were reproduced from the green color somatic embryos on the callus reproduction medium. The obtained transgenic cells were cultured in Murashige and Tucker's liquid medium containing 50 g sucrose $L^{-1}$ in a shaking incubator. Similar to the native miraculin, the secreted recombinant miraculin protein formed a disulfide-linked dimer and retained taste-modifying activity. The stability of recombinant protein expression was confirmed over nine generations of callus. This production system can be an excellent alternative for producing various recombinant proteins as well as miraculin.

플라즈마 처리에 의한 신발용 고무부품의 접착특성 연구

정부영 ( Boo Young Jeong ),천정미 ( Jung Mi Cheon ),이상진 ( Sang Jin Lee ),문진복 ( Jin Bok Moon ),천제환 ( Jae Hwan Chun ) 한국접착및계면학회 2013 접착 및 계면 Vol.14 No.3

본 연구에서는 신발에 사용되는 고무부품의 접착에 있어서 버핑 및 용매 세척 등의 표면처리 공정을 대체하기 위하여, 플라즈마 처리를 통한 표면 및 접착특성을 알아보았다. 플라즈마 처리 시분사노즐과 고무 시편과의 거리가 감소할수록 접촉각은 감소하였으며, 플라즈마 처리속도가 증가함에 따라 접촉각은 증가하였다. 고무 시편의 표면조도 측정결과 플라즈마 처리 후 Ra 값은 20%, Rz 값은 16%가 증가하는 결과를 나타내었다. 플라즈마 발생 노즐과 고무시편과의 거리가 증가함에 따라 접착력은 감소하고, 플라즈마 처리속도가 증가함에 따라 접착력은 감소하였다. In this study, we studied surface and adhesion properties with plasma treatment for substitution of buffing and solvent-cleaning in footwear adhesion process. The distance between nozzle and rubber parts was decreased with decreasing the contact angle. And when a speed of plasma treatment increased, the contact angle increased. The result of surface roughness, Ra and Rz increased in 20% and 16% after the plasma treatment. The distance of between nozzle and rubber parts was decreased with decreasing the peel strength. And the speed of plasma treatment was increased with decreasing the peel strength.

Ophthalmologic manifestations in patients with inflammatory bowel disease

( Hye Jin Lee ),( Hyun Joo Song ),( Jin Ho Jeong ),( Heung Up Kim ),( Sun-jin Boo ),( Soo-young Na ) 대한장연구학회 2017 Intestinal Research Vol.15 No.3

Background/Aims: Inflammatory bowel disease (IBD), including Crohn`s disease (CD) and ulcerative colitis (UC), has been reported to have various ophthalmologic manifestations. The aim of this study was to evaluate the prevalence of ophthalmologic manifestations associated with IBD in Korea. Methods: Sixty-one patients were examined between May 2013 and October 2014. We performed complete ophthalmologic examinations. Results: Findings included 36 patients with CD and 25 with UC. The mean age of the patients was 34±16 years and disease duration was 45.3±23.9 months. Ophthalmologic manifestations were positive in 44 cases. Primary complication was diagnosed in 5 cases, as follows; iritis in 2 cases, episcleritis in one case, iritis with optic neuritis in 1 case, and serous retinal detachment in 1 case, without secondary complications. The most common coincidental complication was dry eye syndrome (DES), in 35 patients (57.4%). The prevalence of DES in the control group was 21.3%. The proportion of DES in patients with IBD was significantly higher than in the control group (P=0.002). Conclusions: Ophthalmologic manifestations were high (72.1%) in IBD patients. Clinically significant primary ocular inflammation occurred in 8.2% of patients. The most common complication was DES. There was a higher rate of DES in patients with IBD compared to the control group. Evaluation of the eye should be a routine component in patients with IBD. (Intest Res 2017;15:380-387)