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RISS 인기검색어
- 검색키워드
- 저자 : Jimin Fu (검색결과 3 건)
- 무료
- 기관 내 무료
- 유료
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A SERS-based lateral flow assay biosensor for highly sensitive detection of HIV-1 DNA
Fu, Xiuli,Cheng, Ziyi,Yu, Jimin,Choo, Priscilla,Chen, Lingxin,Choo, Jaebum Elsevier Applied Science 2016 Biosensors & bioelectronics Vol. No.
<P><B>Abstract</B></P> <P>User-friendly lateral flow (LF) strips have been extensively used for point-of-care (POC) self-diagnostics, but they have some limitations in their detection sensitivity and quantitative analysis because they only identify the high cut-off value of a biomarker by utilizing color changes that are detected with the naked eye. To resolve these problems associated with LF strips, we developed a novel surface-enhanced Raman scattering (SERS)-based LF assay for the quantitative analysis of a specific biomarker in the low concentration range. Herein, human immunodeficiency virus type 1 (HIV-1) DNA was chosen as the specific biomarker. Raman reporter-labeled gold nanoparticles (AuNPs) were employed as SERS nano tags for targeting and detecting the HIV-1 DNA marker, as opposed to using bare AuNPs in LF strips. It was possible to quantitatively analyze HIV-1 DNA with high sensitivity by monitoring the characteristic Raman peak intensity of the DNA-conjugated AuNPs. Under optimized conditions, the detection limit of our SERS-based lateral flow assay was 0.24pg/mL, which was at least 1000 times more sensitive compared to colorimetric or fluorescent detection methods. These results demonstrate the potential feasibility of the proposed SERS-based lateral flow assay to quantitatively detect a broad range of genetic diseases with high sensitivity.</P> <P><B>Highlights</B></P> <P> <UL> <LI> We report a SERS-based lateral flow biosensor for the detection of HIV-1 DNA. </LI> <LI> Quantitative analysis of HIV-1 DNA is possible using this SERS platform. </LI> <LI> This approach provides new insights into the early diagnosis of a HIV-1 disease. </LI> </UL> </P>
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Crystal structure of LRG1 and the functional significance of LRG1 glycan for LPHN2 activation
Yang Jimin,Yin Guo Nan,Kim Do-Kyun,Han Ah-reum,Lee Dong Sun,Min Kwang Wook,Fu Yaoyao,Yun Jeongwon,Suh Jun-Kyu,Ryu Ji-Kan,Kim Ho Min 생화학분자생물학회 2023 Experimental and molecular medicine Vol.55 No.-
The serum glycoprotein leucine-rich ɑ-2-glycoprotein 1 (LRG1), primarily produced by hepatocytes and neutrophils, is a multifunctional protein that modulates various signaling cascades, mainly TGFβ signaling. Serum LRG1 and neutrophil-derived LRG1 have different molecular weights due to differences in glycosylation, but the impact of the differential glycan composition in LRG1 on its cellular function is largely unknown. We previously reported that LRG1 can promote both angiogenic and neurotrophic processes under hyperglycemic conditions by interacting with LPHN2. Here, we determined the crystal structure of LRG1, identifying the horseshoe-like solenoid structure of LRG1 and its four N-glycosylation sites. In addition, our biochemical and cell-biological analyses found that the deglycosylation of LRG1, particularly the removal of glycans on N325, is critical for the high-affinity binding of LRG1 to LPHN2 and thus promotes LRG1/LPHN2-mediated angiogenic and neurotrophic processes in mouse tissue explants, even under normal glucose conditions. Moreover, the intracavernous administration of deglycosylated LRG1 in a diabetic mouse model ameliorated vascular and neurological abnormalities and restored erectile function. Collectively, these data indicate a novel role of LRG1 glycans as molecular switches that can tune the range of LRG1’s cellular functions, particularly the LRG1/LPHN2 signaling axis.
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Xiao Tian,Tszyin Poon,Yiyi Yang,Hong Hu,Jimin Fu,Jianhui Li,Ben Niu 한국섬유공학회 2022 Fibers and polymers Vol.23 No.9
Chitosan fiber possesses inherent advantages, such as excellent biocompatibility, nontoxicity as well asantibacterial property. However, there is no systematic study on the chitosan fiber blending effect with other fibers, and onthat basis, there is no suitable fiber blending technology for yarn and fabric production, which results in the incorrectproportion of chitosan fiber blended with other fiber type and thus lowers the fabric functionality as well as increases thematerial cost. In this study, thirteen chitosan blended yarns were designed and developed. Blending yarn structure wasexamined and the effects of blending fiber type and ratio on the antibacterial and physical property were firstly investigated. The experimental results show that the inhibition rate rises with the increase of the chitosan blending ratio. The polyester/cotton/chitosan and tencel/chitosan yarns have a better inhibition rate of Staphylococcus than that of the cotton/chitosan yarn,indicating that the blending fiber type has an impact on antibacterial properties. Moreover, various chitosan blended knittedfabrics were produced by using the chitosan blended yarns developed and their antibacterial, physical properties and comfortas well as anti-mite activity were evaluated. The results illustrate that the knitted fabrics with a small amount of chitosanfibers exhibit excellent antibacterial properties and desirable comfort. Furthermore, the chitosan blended fabrics present theanti-mites effect. This work would contribute to lowering the material cost of functional yarns and promoting thedevelopment of antibacterial textiles.
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