Effectiveness of the Endplate Reduction Technique Combined With Bone Grafting for the Treatment of Thoracolumbar Fractures by Using Posterior Short-Segment Fixation

Jiguang Hou,Dong Ren,Yufeng Chen,Lindan Geng,Shuangquan Yao,Haotian Wu,Pengcheng Wang 대한척추신경외과학회 2023 Neurospine Vol.20 No.1

Objective: This study aimed to examine the effect of the endplate reduction (EPR) technique combined with bone grafting for treating thoracolumbar burst fractures using posterior shortsegmental fixation. Methods: Patients with thoracolumbar fractures admitted between January 2018 and October 2021 were retrospectively analyzed, and those meeting the criteria were assigned to the EPR group and the intermediate screws (IS) group. The vertebral wedge angle (VWA), Cobb angle (CA), anterior vertebral body height (AVBH), middle vertebral body height (MVBH), upper endplate line (UEPL), upper intervertebral angle (UIVA), and upper intervertebral disc height (UIDH) indices were examined and compared preoperatively, first day postoperatively, as well as at 12 months postoperatively. Results: The result indicated that the EPR group achieved better MVBH reduction (p < 0.001), UEPL reduction (p < 0.001), vertebral body fracture healing (p = 0.006), as well as implant breakage (p = 0.04) than the IS group; VWA (p < 0.001), CA (p = 0.005), AVBH (p < 0.001), MVBH (p < 0.001), UEPL (p < 0.001), and UIDH (p < 0.001) were lost after reduction less than those in the IS group. There was no significant difference in operative time (p = 0.315) and intraoperative bleeding (p = 0.274) between the 2 groups. Conclusion: The EPR group achieved better results in repositioning and maintaining MVBH and endplate morphology, with less correction loss after the reduction of the VWA, CA, AVBH, and endplate morphology. The EPR group exhibited a better healing pattern after vertebral fracture and disc degeneration was better relieved.

Spatial Distribution and Influencing Factor of Soil Moisture in Typical Depression Area of Karst Region

Jiguang Zhang,Yirong Su,Hongsong Chen,Xiangli Kong,Wei Zhang,Jiuquan Zhang,Hongbo Liang 한국토양비료학회 2014 한국토양비료학회 학술발표회 초록집 Vol.2014 No.6

Growth and Triterpenic Acid Accumulation of Cyclocarya paliurus Cell Suspension Cultures

Zhongping Yin,Xinchen Shangguan,Jiguang Chen,Qiang Zhao,Dongming Li 한국생물공학회 2013 Biotechnology and Bioprocess Engineering Vol.18 No.3

Cyclocarya paliurus is a unique plant growing in central China with hypoglycaemic and hypolipaemia effects. To make better use of this functional food resource,cell suspension cultures and triterpenic acid accumulation were studied. Stable and uniform cell suspension cultures were established in liquid basal Murashige and Skoog medium supplemented with 2,4-dichlorophenoxy acetic acid (0.5 mg/L), naphthalene acetic acid (0.3 mg/L) and cytokinin (1.0 mg/L). According to the growth curve and triterpenic acid accumulation curve, the 8 ~ 10th day postinoculation was the optimum time for subculture, and the 14th day was the optimum time for harvest. Murashige and Skoog medium and woody plant medium were suitable for both cell growth and triterpenic acid accumulation. 3%sucrose (w/v), 60 mM total nitrogen (NO3-/NH4+ = 2/1),1.25 mM KH2PO4, 2 mM CaCl2, and 2 mM MgSO4 were all found to be fit for cell growth and triterpenic acid accumulation in a cell suspension culture of Cyclocarya paliurus. Total triterpenic acid, ursolic acid and oleanolic acid content in suspended cultured cells were all significantly higher than that of leaves and calluses (P 0.01), with levels up to 6.24, 2.28, and 0.94% (of dry weight), respectively. The betulinic acid content of suspended cultured cells also reached 0.82%, which was significantly higher than that of calluses. These results suggest that suspended cultured cells of Cyclocarya paliurus were rich in triterpenic acids and could be used for the production of total triterpenic acid, ursolic acid, oleanolic acid and betulinic acid.

Smooth Path Planning Method for Unmanned Surface Vessels Considering Environmental Disturbance

Jiabin Yu,Zhihao Chen,Zhiyao Zhao,Xiaoyi Wang,Yuting Bai,Jiguang Wu,Jiping Xu 제어·로봇·시스템학회 2023 International Journal of Control, Automation, and Vol.21 No.10

To solve the problems of unsmooth path planning, insufficient dynamic obstacle avoidance ability, and environmental disturbance effect on the path planning result, this paper proposes a smooth path planning method for unmanned surface vessels (USVs) considering environmental disturbance. First, an improved A* algorithm, which uses the path smoothing method based on the minimum turning radius of a USV, is proposed for global path planning. The binary tree method is used instead of the enumeration method to select a relatively optimal path in the current situation to improve algorithm efficiency. In addition, the dynamic window approach (DWA) with the Convention on the International Regulation for Preventing Collision at Sea (COLREGs) constraints is used for local path planning. The dist function in the DWA algorithm is improved to enhance the DWA algorithm’s ability to avoid dynamic obstacles. Finally, the environmental disturbance function is derived and added to the A* and DWA algorithms to handle the effect of environmental disturbances, such as water flow, on the path planning result, which can significantly improve the path-planning ability of the algorithm in the presence of environmental disturbances. Simulation experiments are performed in three scenarios to verify the proposed algorithm. The experimental results show that compared with the other algorithms, the proposed algorithm can effectively avoid dynamic obstacles and reduce the impact of environmental disturbance on the path planning result. At the same time, the proposed algorithm has high efficiency and strong robustness.

Processing, digestion property and structure characterization of slowly digestible gorgon nut starch

Jia-cheng Zeng,Pin-Jian Xiao,Li-Juan Ling,Li Zhang,Dao-Bang Tang,Qing-Feng Zhang,Jiguang Chen,Jing-En Li,Zhongping Yin 한국식품과학회 2022 Food Science and Biotechnology Vol.31 No.1

Slowly digestible gorgon nut starch (GN-SDS) was prepared by heating–cooling treatment (HCT), meanwhile its morphological and structural features were characterized in detail by SEM, DSC, XRD and IR detection. The optimized parameters of GN-SDS processing were as following: starch milk (20%) was heated at 100 °C for 20 min, and then cooled under 4 °C for 24 h. Under the optimized parameters, the SDS content increased from 20.49 to 61.74%. GN-SDS showed typical SDS characteristics in in vivo digestion with a low postprandial blood glucose. SEM images suggested that GN-S particles changed from uniform regular polyhedron with smooth surface to irregular gravel-like particles with coarse surface and obvious layered structure inside after HCT. The results of SEM, DSC, XRD and IR determination indicated that HCT changed the granule morphology, interior structure, gelatinization temperature and crystal type (A to B-type) of GN-S, and therefore made it hard to be digested accordingly.

Development of Origanum vulgare Cell Suspension Culture to Produce Polyphenols and the Stimulation Effect of Salicylic Acid Elicitation and Phenylalanine Feeding

Yan-Ping Li,Dao-Bang Tang,Xiao-Qiang Wang,Meng Wang,Qing-Feng Zhang,Yuan Liu,Bei-Yun Shen,Jiguang Chen,Zhongping Yin 한국생물공학회 2021 Biotechnology and Bioprocess Engineering Vol.26 No.3

Three types of calli were induced from Origanum vulgare (O. vulgare) aseptic seedlings, and the friable calli with white appearance and high growth rate were further screened and used to develop cell suspension culture to produce polyphenols. Murashige and Skoog (MS) medium with 3.0 mg/L Kinetin (KT) and 0.5 mg/L 2,4-dichlorophenoxy acetic acid (2,4-D) was suitable for both O. vulgare cells growth and polyphenols accumulation. To further enhance the polyphenols accumulation, O. vulgare cells were treated by phenylalanine (Phe) feeding and salicylic acid (SA) elicitation. Compared with the individual Phe feeding and SA elicitation, SA elicitation combined with Phe feeding showed a much better promotion effect on the polyphenols synthesis in O. vulgare cells, especially rosmarinic acid (RosA) accumulation. With the combined treatment of 200 μM SA and 100 μM Phe, total polyphenols content and yield were 41.36 mg/g and 752.93 mg/L, respectively. RosA content and yield reached 31.25 mg/g and 570.37 mg/L, which were 5.44 and 5.47 times that of the control. Furthermore, the total polyphenols extracted from the cultured cells treated by SA elicitation combined with Phe feeding displayed a much higher antioxidant capacity than that of untreated cells, meanwhile its 1,1-diphenyl-2- trinitrophenyl hydrazine (DPPH) and superoxide anion radical-scavenging activity were much stronger than that of vitamin C. What’s more, our results also showed that RosA was the principal contributor to the fine antioxidant capacity of the total polyphenols extracted from the SA and Phe treated cells. Our research indicated that SA elicitation combined with Phe feeding significantly improved the polyphenols yield and antioxidant capacity of the cultured O. vulgare cells, and therefore has a promising application prospect in natural polyphenols production.