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A Nanofilter Array Chip for Fast Gel-Free Biomolecule Separation.
Fu, Jianping,Mao, Pan,Han, Jongyoon American Institute of Physics 2005 APPLIED PHYSICS LETTERS Vol.87 No.26
<P>We report here a microfabricated nanofilter array chip that can size-fractionate SDS-protein complexes and small DNA molecules based on the Ogston sieving mechanism. Nanofilter arrays with a gap size of 40-180nm were fabricated and characterized. Complete separation of SDS-protein complexes and small DNA molecules were achieved in several minutes with a separation length of 5mm. The fabrication strategy for the nanofilter array chip allows further increasing of the nanofilter density and decreasing of the nanofilter gap size, leading, in principle, to even faster separation.</P>
Jixiang Xu,Jianjun Wang,Jianping Han,Zhaolong Han,Jiahuang Tu,Zhanjie Li 대한토목학회 2022 KSCE JOURNAL OF CIVIL ENGINEERING Vol.26 No.4
The aim of this paper is to investigate the bolted end-plate beam to column (BEBC) joints with different strengthening strategies under fire through a parametric study by using a validated computational model. The current studies are mainly more focused on the mechanical behavior of a certain kind of reinforced or unreinforced BEBC joints at either room or fire alone, limiting investigations are available for a comprehensive stiffening strategy study of the BEBC joints under fire. Therefore, there is a need in improving the performance of BEBC joints with different stiffening strategies, the fire resistance comparison of these BEBC joints with different strengthening strategies under fire is of great interest. The analyses of the fire resistance behavior of the stiffened or unstiffened BEBC joints under fire were conducted by using a developed model that was established and validated by using the existing test results. A parametric study was conducted for BEBC joints with web stiffeners, end-plate stiffeners and web and end-plate stiffeners together under fire using the validated computational model. In order to better compare the fire resistance of BEBC joints with different strengthening strategies under a fire, 24 finite element models were established by considering different strengthening strategies and end-plate thicknesses. The results on different strengthening strategies and the end-plate thicknesses revealed the major impact of web stiffeners and end-plate web stiffener together, and the sensitivities of end-plate thickness for unreinforced BEBC joints. For the fire resistance of unreinforced and stiffened BEBC joints, the findings herein can provide a valuable reference for potential design recommendations.
Yingran Liang,Yuanyuan Zhang,Miaomiao Liu,Xue Han,Jianping Zhang,Xuan Zhang,Li Chu 대한약학회 2020 Archives of Pharmacal Research Vol.43 No.8
Many studies describe the stimulating eff ect ofquercetin on Ca 2+ channels and the treatment of cardiovasculardiseases such as myocardial ischemia and hypertension. However, these studies are scattered and contradictory. The aim of this study is to elucidate the protective eff ects ofquercetin against isoproterenol (ISO)-induced myocardialischemia and verify the cellular mechanisms based on theL-type Ca 2+ channel (LTCC), Ca 2+ transients, and myocardialcontractility. An animal model of myocardial ischemiawas established by subcutaneous injection of ISO for 2 days. Quercetin significantly reduced J-point elevation, heartrate, reactive oxygen species, serum levels of myocardialenzymes, superoxide dismutase, catalase, glutathione, glutathioneperoxidase, glutathione S-transferase and improvedheart pathologic morphology. L-type Ca 2+ current (I Ca-L )was tested in an experiment with isolated rat myocardialcells by using the whole-cell patch-clamp recording techniqueand IonOptix Myocam detection system. Quercetin reduced I Ca-L in a concentration-dependent fashion with ahalf-maximal inhibitory concentration of 4.67 × 10 –4 M. Quercetin also shifted the current–voltage curve upwards,moved the activation and inactivation curves to the left andinhibited the amplitude of the cell shortening and Ca 2+ transients. The results showed that quercetin acts as a LTCCinhibitor and exerts a cardioprotective eff ect by inhibitingCa 2+ infl ux and contractility in rats.
Yue Lin,Yuanyuan Zhang,Qiongtao Song,Tao Song,Xue Han,Ying Zhang,Xuan Zhang,Xi Chu,Fenghua Zhang,Li Chu,Jianping Zhang 대한약학회 2017 Archives of Pharmacal Research Vol.40 No.10
To explore the cardiovascular protective effectsof Magnesium isoglycyrrhizinate (MI), especially theunderlying cellular mechanisms related to L-type calciumchannels and myocardial contractility, and to examine theeffects of MI on hERG K? current expressed in HEK293cells. We used the whole-cell patch clamp technique,video-based edge detection and dual excitation fluorescencephotomultiplier systems to explore the effect of MIon L-type Ca2? currents (ICa-L) and cell contraction in ratcardiomyocytes. We also examined the rapidly activatingdelayed rectifier potassium current (IKr) expressed inHEK293 cells using a perforated patch clamp. MI inhibitedICa-L in a dose-dependent manner, with a half-maximalinhibitory concentration (IC50) of 0.22 mg/ml, and themaximal inhibitory effect was 61.10 ± 0.59%. MI at aconcentration of 0.3 mg/ml reduced cell shortening by24.12 ± 3.97% and the peak value of the Ca2? transient by36.54 ± 4.96%. MI had no significant influence on hERGK? channels expressed in HEK293 cells at all testpotentials. MI exerts protective effects on the heart via theinhibition of ICa-L and cell shortening in rat cardiomyocytes. However, MI had no significant influence on IKr;thus, MI may exert cardioprotective effects without causingdrug-induced long QT syndrome.